TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC145329.2 + phase: 0 /pseudo
         (246 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC8301                                                                 65  1e-11
TC9451 homologue to UP|O24457 (O24457) Pyruvate dehydrogenase E1...    27  2.5
TC19498                                                                25  9.4
NP566648 hypothetical protein [Lotus japonicus]                        25  9.4
CN825203                                                               25  9.4
TC12115                                                                25  9.4

>TC8301 
          Length = 1243

 Score = 64.7 bits (156), Expect = 1e-11
 Identities = 38/103 (36%), Positives = 59/103 (56%)
 Frame = +2

Query: 2   MGSKWDIEKFTGDNDFGLWKVKMEAVLIQQKCEKALKGEGLLPVTMSQVEKTDMVDKVRS 61
           M +K++I KF+G N F LWK+K++A+L +  C  A+ G    P  ++  +  +M D   +
Sbjct: 500 MAAKFEIPKFSGSN-FSLWKLKIKAILRKDNCLPAIDGR---PADITDDKWKEMDDNAVA 667

Query: 62  AIVLCLGDKVLREVAKEPTAASIWAKLESLYMTKSLAHRQFLK 104
            + L + D VL  +A++ TA  IW  L  LY  KSL H + LK
Sbjct: 668 NLHLAVADSVLSSIAEKKTAKEIWDTLIQLYEVKSL-HNRILK 793


>TC9451 homologue to UP|O24457 (O24457) Pyruvate dehydrogenase E1 alpha
           subunit  (At1g01090/T25K16_8) , partial (52%)
          Length = 1082

 Score = 27.3 bits (59), Expect = 2.5
 Identities = 14/27 (51%), Positives = 15/27 (54%)
 Frame = -3

Query: 197 GEGLSVLRGNDGGRGNRRKSGNKSRSE 223
           GE L V+   DGG   RRK G   RSE
Sbjct: 288 GERLVVIGSGDGGIDRRRKVGEFGRSE 208


>TC19498 
          Length = 634

 Score = 25.4 bits (54), Expect = 9.4
 Identities = 12/22 (54%), Positives = 13/22 (58%)
 Frame = +3

Query: 208 GGRGNRRKSGNKSRSECFNCHK 229
           GGR N   SG  S + CFN HK
Sbjct: 93  GGRSNDWLSGICSSAFCFNVHK 158


>NP566648 hypothetical protein [Lotus japonicus]
          Length = 1101

 Score = 25.4 bits (54), Expect = 9.4
 Identities = 19/74 (25%), Positives = 34/74 (45%)
 Frame = +1

Query: 29  IQQKCEKALKGEGLLPVTMSQVEKTDMVDKVRSAIVLCLGDKVLREVAKEPTAASIWAKL 88
           ++ +CE+ALK   LL       E     +KV   I   +   +L  + +   A S++ KL
Sbjct: 736 LKSECEEALKDGDLLMNAGKLKEALPYYEKVMDKITFKISKHILM-IDRSNEAQSMYEKL 912

Query: 89  ESLYMTKSLAHRQF 102
           +S    K++   +F
Sbjct: 913 KSHPNAKAMEMMKF 954


>CN825203 
          Length = 644

 Score = 25.4 bits (54), Expect = 9.4
 Identities = 10/14 (71%), Positives = 12/14 (85%)
 Frame = -1

Query: 195 EHGEGLSVLRGNDG 208
           EHGEG+SVL+G  G
Sbjct: 497 EHGEGISVLQGGFG 456


>TC12115 
          Length = 575

 Score = 25.4 bits (54), Expect = 9.4
 Identities = 10/27 (37%), Positives = 14/27 (51%)
 Frame = -2

Query: 189 KDLRVHEHGEGLSVLRGNDGGRGNRRK 215
           KDL +H    G  V+    GG+GN  +
Sbjct: 394 KDLIIHVRENGAKVIEEGGGGKGNTNR 314


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.323    0.138    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,454,332
Number of Sequences: 28460
Number of extensions: 34387
Number of successful extensions: 194
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of query: 246
length of database: 4,897,600
effective HSP length: 88
effective length of query: 158
effective length of database: 2,393,120
effective search space: 378112960
effective search space used: 378112960
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 54 (25.4 bits)