BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145329.1 - phase: 0
(236 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11480 similar to UP|Q8VYW5 (Q8VYW5) AT5g45290/K9E15_7, partial... 32 0.13
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 30 0.37
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 30 0.48
BP048490 29 0.62
BP034907 29 0.62
BP034146 29 0.81
BP043430 28 1.1
AI967639 28 1.1
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 28 1.4
TC7830 UP|Q8H946 (Q8H946) Phosphoenolpyruvate carboxylase , com... 28 1.8
TC8964 weakly similar to UP|Q94F54 (Q94F54) AT3g15070/K15M2_22, ... 27 3.1
TC8679 homologue to UP|Q43466 (Q43466) Protein kinase 3 , partia... 27 3.1
BP048961 27 3.1
BP077937 27 3.1
TC8582 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetyla... 27 4.0
BP036655 27 4.0
AV420361 26 5.3
AV415462 26 5.3
AV427045 26 5.3
TC13834 homologue to UP|Q948R9 (Q948R9) RPS6-like protein (AT3g1... 26 5.3
>TC11480 similar to UP|Q8VYW5 (Q8VYW5) AT5g45290/K9E15_7, partial (8%)
Length = 502
Score = 31.6 bits (70), Expect = 0.13
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +2
Query: 97 FDLNSVWKGEQESGDNDGDECDVCRVDEEVDDE 129
F L S KG+ +GD+D ++C +C + E D+
Sbjct: 62 FPLKSHSKGDAANGDHDAEQCHICLAEYEEGDQ 160
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 30.0 bits (66), Expect = 0.37
Identities = 12/30 (40%), Positives = 22/30 (73%)
Frame = -2
Query: 105 GEQESGDNDGDECDVCRVDEEVDDENEDEE 134
G +E+G+++ DE + D++ DDE++DEE
Sbjct: 449 GPEENGEDEEDEDGEDQEDDDDDDEDDDEE 360
Score = 28.1 bits (61), Expect = 1.4
Identities = 13/29 (44%), Positives = 17/29 (57%)
Frame = -2
Query: 106 EQESGDNDGDECDVCRVDEEVDDENEDEE 134
E E G++ D+ D D+E DD EDEE
Sbjct: 422 EDEDGEDQEDDDDDDEDDDEEDDGGEDEE 336
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 29.6 bits (65), Expect = 0.48
Identities = 20/71 (28%), Positives = 34/71 (47%)
Frame = +3
Query: 106 EQESGDNDGDECDVCRVDEEVDDENEDEEIRFDRESFSRMLRRVTLVEARMYAHMSHLGN 165
E E D+D D+ D +E+ DD++E+EE + E + + + A +G
Sbjct: 288 EDEDDDDDDDDDDDGEEEEDDDDDDEEEEGSEEVEVEGKDGAKGAAAQTTKAAAAMCVGI 467
Query: 166 LAYSIPNIKQG 176
++S PN QG
Sbjct: 468 GSFSDPNEAQG 500
Score = 27.7 bits (60), Expect = 1.8
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +3
Query: 106 EQESGDNDGDECDVCRVDEEVDDENEDEE 134
+ E D+D D+ D +EE DD+++DEE
Sbjct: 282 DDEDEDDDDDDDDDDDGEEEEDDDDDDEE 368
Score = 26.2 bits (56), Expect = 5.3
Identities = 10/29 (34%), Positives = 20/29 (68%)
Frame = +3
Query: 106 EQESGDNDGDECDVCRVDEEVDDENEDEE 134
+++ D+D D+ D +EE DD++++EE
Sbjct: 285 DEDEDDDDDDDDDDDGEEEEDDDDDDEEE 371
>BP048490
Length = 452
Score = 29.3 bits (64), Expect = 0.62
Identities = 22/94 (23%), Positives = 41/94 (43%), Gaps = 16/94 (17%)
Frame = +3
Query: 106 EQESGDNDGD-ECDVCRVDEEVDDENEDEEIRFDRESFSRMLRRVTLVEARMYAHMS--- 161
+++S D+D + + D VDE E+ DEE ++ + ++ V +V+A +
Sbjct: 135 DEDSSDSDSEVKKDKMDVDESDSSEDSDEEDEKSTKTPQKKVKDVEMVDASSGKNAPKTP 314
Query: 162 ------------HLGNLAYSIPNIKQGNLLKRCG 183
+GNL++S+ N K CG
Sbjct: 315 AAQTENEGSKTLFVGNLSFSVQRSDVENFFKDCG 416
>BP034907
Length = 448
Score = 29.3 bits (64), Expect = 0.62
Identities = 19/53 (35%), Positives = 29/53 (53%), Gaps = 4/53 (7%)
Frame = +3
Query: 9 SGIPGIVP--LITSISATTTTSRAN--DHVHVHQSHVTTVRRSNKSSMFSRFI 57
SG I P L+T SA TS + DH+ +++H+T+V SN S+ S +
Sbjct: 258 SGASDIRPQELVTFASAEAETSSSTEIDHISSNRNHLTSVESSNTSTSASAHV 416
>BP034146
Length = 465
Score = 28.9 bits (63), Expect = 0.81
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = -3
Query: 15 VPLITSISATTTTSRANDHVHVHQSHVTTVRRSNKSSMFS 54
VPLI + T+T + + + V H++T R KSS+F+
Sbjct: 391 VPLIMKETQTSTELQKREKIQVIPGHLSTHMRLQKSSLFA 272
>BP043430
Length = 493
Score = 28.5 bits (62), Expect = 1.1
Identities = 10/31 (32%), Positives = 21/31 (67%)
Frame = -1
Query: 104 KGEQESGDNDGDECDVCRVDEEVDDENEDEE 134
+G + D++G++ +V DEE +D N+D++
Sbjct: 370 EGSGKDNDDEGNDNEVDEEDEEEEDSNDDDD 278
>AI967639
Length = 335
Score = 28.5 bits (62), Expect = 1.1
Identities = 17/59 (28%), Positives = 35/59 (58%), Gaps = 1/59 (1%)
Frame = +1
Query: 98 DLNSVWKGEQESGDND-GDECDVCRVDEEVDDENEDEEIRFDRESFSRMLRRVTLVEAR 155
D++S +G++E + D GDE ++ +E+ DD+ +D+++ +S S R+V + R
Sbjct: 79 DISSSSEGDEEDEEEDQGDEYNLEDEEEDDDDD-DDDDLSISEDSDSDKPRKVKQLPGR 252
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 28.1 bits (61), Expect = 1.4
Identities = 24/80 (30%), Positives = 37/80 (46%), Gaps = 2/80 (2%)
Frame = +2
Query: 57 IGSSARNNCLAAVNDAFT--AENADRTVKEGDGQNGNWVFKVFDLNSVWKGEQESGDNDG 114
IGS+ R+ + FT AE +D E D ++G+ E E D+D
Sbjct: 686 IGSTIRDKIIPHAVSWFTGEAEQSDFEDIEEDDEDGD--------------EDEDEDDDD 823
Query: 115 DECDVCRVDEEVDDENEDEE 134
DE +E+ DD++ED+E
Sbjct: 824 DE-----EEEDDDDDDEDDE 868
>TC7830 UP|Q8H946 (Q8H946) Phosphoenolpyruvate carboxylase , complete
Length = 3376
Score = 27.7 bits (60), Expect = 1.8
Identities = 20/93 (21%), Positives = 46/93 (48%), Gaps = 8/93 (8%)
Frame = +2
Query: 118 DVCRVDEEVDDENEDEEIRFDRESFSRMLRRVT-------LVEARMYAHMSHLGNLAYSI 170
D+ +EV + + + E + D E + + +T +V A+ ++HM +L NLA +
Sbjct: 251 DLKETVQEVYELSAEYERKHDHEKLEELGKVITSLDAGDSIVVAKSFSHMLNLANLAEEV 430
Query: 171 P-NIKQGNLLKRCGLRFVTSSIEKKELAASIKK 202
+ ++ N LK+ ++ + ++ ++KK
Sbjct: 431 QISRRRRNKLKKGDFADENNATTESDIEETLKK 529
>TC8964 weakly similar to UP|Q94F54 (Q94F54) AT3g15070/K15M2_22, partial
(19%)
Length = 594
Score = 26.9 bits (58), Expect = 3.1
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +1
Query: 101 SVWKGEQESGDNDGDECDVCRVDEEVDDENEDEEIRFDRESFSRMLRRVTLVE 153
SV + E S D + D C +C+ +E ++ E +R + E + LR+ LV+
Sbjct: 148 SVNEEEAASNDQETDSCMICQ--DEFKNQEEIGILRCEHEYHAECLRKWLLVK 300
>TC8679 homologue to UP|Q43466 (Q43466) Protein kinase 3 , partial (70%)
Length = 972
Score = 26.9 bits (58), Expect = 3.1
Identities = 17/37 (45%), Positives = 24/37 (63%)
Frame = +2
Query: 103 WKGEQESGDNDGDECDVCRVDEEVDDENEDEEIRFDR 139
W+GE+E DE DV +EEV++E EDEE +D+
Sbjct: 632 WEGEEE------DEEDV---EEEVEEE-EDEEDEYDK 712
>BP048961
Length = 543
Score = 26.9 bits (58), Expect = 3.1
Identities = 14/32 (43%), Positives = 19/32 (58%)
Frame = -2
Query: 105 GEQESGDNDGDECDVCRVDEEVDDENEDEEIR 136
GE+E +G+E RV E ++E EDEE R
Sbjct: 164 GEEEEEGEEGEEGP*WRVWESGEEEEEDEEGR 69
>BP077937
Length = 469
Score = 26.9 bits (58), Expect = 3.1
Identities = 13/27 (48%), Positives = 14/27 (51%)
Frame = -3
Query: 90 GNWVFKVFDLNSVWKGEQESGDNDGDE 116
G WVF N G E GD+DGDE
Sbjct: 392 GKWVFS----NGACDGGWEDGDDDGDE 324
>TC8582 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetylase
HD2-P39, partial (65%)
Length = 1341
Score = 26.6 bits (57), Expect = 4.0
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +1
Query: 109 SGDNDGDECDVCRVDEEVDDENEDE 133
S D G E D+ DE+ DDE++DE
Sbjct: 568 SPDVSGYEDDLISADEDSDDESDDE 642
>BP036655
Length = 556
Score = 26.6 bits (57), Expect = 4.0
Identities = 14/36 (38%), Positives = 22/36 (60%)
Frame = -3
Query: 106 EQESGDNDGDECDVCRVDEEVDDENEDEEIRFDRES 141
E+E +DGD+ +V +EEV +E D++ D ES
Sbjct: 410 EEEGLSDDGDDDEVVDEEEEVVEEWSDDDDINDDES 303
>AV420361
Length = 250
Score = 26.2 bits (56), Expect = 5.3
Identities = 9/22 (40%), Positives = 13/22 (58%)
Frame = -3
Query: 96 VFDLNSVWKGEQESGDNDGDEC 117
+F N WK E+ G+N+G C
Sbjct: 71 IFPENGKWKREENWGENEGARC 6
>AV415462
Length = 418
Score = 26.2 bits (56), Expect = 5.3
Identities = 18/61 (29%), Positives = 29/61 (47%)
Frame = -3
Query: 68 AVNDAFTAENADRTVKEGDGQNGNWVFKVFDLNSVWKGEQESGDNDGDECDVCRVDEEVD 127
A +D T + A E DG +G V +V W+ + + D+ GD+ +V DE D
Sbjct: 179 AFDDVLTVDTAGH---EDDGLHGAGV-RVLGGADAWRLDDDVVDDAGDDHEVGEEDESED 12
Query: 128 D 128
+
Sbjct: 11 E 9
>AV427045
Length = 414
Score = 26.2 bits (56), Expect = 5.3
Identities = 19/49 (38%), Positives = 24/49 (48%), Gaps = 6/49 (12%)
Frame = -1
Query: 27 TSRANDHVHVHQSHVTTVRRSNKSSMFSRFIGSS------ARNNCLAAV 69
T N HQSH+ ++ N+ S FS F GSS + CLAAV
Sbjct: 339 TQLLNAKTITHQSHIKLLQFLNRCSEFSDF-GSSDPEAAVGKPACLAAV 196
>TC13834 homologue to UP|Q948R9 (Q948R9) RPS6-like protein
(AT3g17170/K14A17_29), partial (13%)
Length = 517
Score = 26.2 bits (56), Expect = 5.3
Identities = 11/37 (29%), Positives = 20/37 (53%)
Frame = +1
Query: 98 DLNSVWKGEQESGDNDGDECDVCRVDEEVDDENEDEE 134
D + W GE+++ DGD + +D++ DD + E
Sbjct: 181 DYDDDWDGEEDADGEDGD-AEFIVIDDDQDDVDSGNE 288
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.129 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,450,433
Number of Sequences: 28460
Number of extensions: 40455
Number of successful extensions: 333
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 278
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 315
length of query: 236
length of database: 4,897,600
effective HSP length: 87
effective length of query: 149
effective length of database: 2,421,580
effective search space: 360815420
effective search space used: 360815420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC145329.1