BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145222.11 + phase: 0 /pseudo
(394 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB827219 247 3e-68
TC8353 homologue to UP|HS7M_PHAVU (Q01899) Heat shock 70 kDa pro... 95 2e-20
BP040870 89 2e-18
BP041494 65 1e-17
TC9795 homologue to UP|Q9ATB8 (Q9ATB8) BiP-isoform D (Fragment),... 84 5e-17
TC14233 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (67%) 82 2e-16
BP044919 74 4e-14
TC14234 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (66%) 73 9e-14
AW720237 68 2e-12
TC18168 homologue to UP|Q8GSN4 (Q8GSN4) Non-cell-autonomous heat... 68 3e-12
AV769455 65 1e-11
BP064075 60 8e-10
AV409958 42 2e-04
BP083571 37 0.004
BP062486 28 2.0
AV766443 28 2.6
AV769359 28 2.6
TC16989 similar to PIR|S66350|S66350 lycopene beta-cyclase - to... 27 4.5
CB827317 27 4.5
BG662417 27 7.7
>CB827219
Length = 544
Score = 247 bits (631), Expect(2) = 3e-68
Identities = 123/148 (83%), Positives = 133/148 (89%)
Frame = +3
Query: 230 LIFNMGAGYCDVAVTATAGGVSQIKALAGSTIGGEDLLQNMMRHLLPDSENIFKRHGVEE 289
L FNMGAGYCDVAVT TAGGVSQIKALAGSTIGGEDLLQNMM HLLPD ENIFK HGV+E
Sbjct: 45 LYFNMGAGYCDVAVTVTAGGVSQIKALAGSTIGGEDLLQNMMHHLLPDCENIFKNHGVKE 224
Query: 290 IKSMALLRVATQDAIHQLSTQSSVQVDVDLGNSLKICKVVDRAEFEEVNKEVFEKCESLI 349
IKSM LLRVAT DAIH+LS+Q SVQVDVDLG+ LKI KV+D EFEEVNK VFEKCESLI
Sbjct: 225 IKSMGLLRVATLDAIHRLSSQKSVQVDVDLGDGLKINKVIDCEEFEEVNKNVFEKCESLI 404
Query: 350 IQCLHDAKVEVGDINDVILVGGCSYIPQ 377
IQCL D+K+EV D+NDVI+VGGCSYIP+
Sbjct: 405 IQCLQDSKIEVEDVNDVIIVGGCSYIPR 488
Score = 28.1 bits (61), Expect(2) = 3e-68
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +2
Query: 215 KASQETMGSGSEKIAL 230
+AS E MGSGSEKIAL
Sbjct: 2 QASHENMGSGSEKIAL 49
>TC8353 homologue to UP|HS7M_PHAVU (Q01899) Heat shock 70 kDa protein,
mitochondrial precursor, partial (74%)
Length = 1752
Score = 95.1 bits (235), Expect = 2e-20
Identities = 61/237 (25%), Positives = 120/237 (49%), Gaps = 6/237 (2%)
Frame = +3
Query: 158 ETHLKRPIRNVVLTVPVSFSRFQLTRLERACAMAGLHVLRLMPEPTAVALLYGQQQQKAS 217
E +L + + V+TVP F+ + A +AGL V R++ EPTA AL YG
Sbjct: 12 EAYLGKSVSKAVITVPAYFNDAHRQATKDAGRIAGLDVQRIINEPTAAALSYGM------ 173
Query: 218 QETMGSGSEKIALIFNMGAGYCDVAVTATAGGVSQIKALAGST-IGGEDLLQNMMRHLLP 276
+ E + +F++G G DV++ + GV ++KA G T +GGED ++ L+
Sbjct: 174 -----TNKEGLIAVFDLGGGTFDVSILEISNGVFEVKATNGDTFLGGEDFDNALLDFLVD 338
Query: 277 DSENIFKRHGVEEIKSMALLRVATQDAIHQLSTQSSVQVDV-----DLGNSLKICKVVDR 331
+ + ++ ++ LR A + A +LS+ S ++++ D + + + R
Sbjct: 339 EFKRTESIDLSKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTR 518
Query: 332 AEFEEVNKEVFEKCESLIIQCLHDAKVEVGDINDVILVGGCSYIPQGWRILLPTYAK 388
++FE + + E+ ++ CL DA + + ++++V+LVGG + +P+ ++ + K
Sbjct: 519 SKFEALVNHLIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVSAIFGK 689
>BP040870
Length = 505
Score = 88.6 bits (218), Expect = 2e-18
Identities = 55/161 (34%), Positives = 92/161 (56%), Gaps = 3/161 (1%)
Frame = +2
Query: 143 EEVLAMFLVELRLMTETHLKRPIRNVVLTVPVSFSRFQLTRLERACAMAGLHVLRLMPEP 202
EE+ +M L++++ + E +L I+N V+TVP F+ Q + A ++GL+V+R++ EP
Sbjct: 41 EEISSMVLMKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEP 220
Query: 203 TAVALLYGQQQQKASQETMGSGSEKIALIFNMGAGYCDVAVTATAGGVSQIKALAGST-I 261
TA A+ YG ++ S EK LIF++G G DV++ G+ ++K+ AG T +
Sbjct: 221 TAAAIAYGLDKKATST------GEKNVLIFDLGGGTFDVSLLTIEEGIFEVKSTAGDTHL 382
Query: 262 GGEDLLQNMMRHLLPDSENIFKRHGVEEI--KSMALLRVAT 300
GGED M+ H + + FKR ++I + AL R+ T
Sbjct: 383 GGEDFDNRMVNHFVQE----FKRKNKKDISGNARALRRLRT 493
>BP041494
Length = 572
Score = 65.1 bits (157), Expect(2) = 1e-17
Identities = 41/109 (37%), Positives = 63/109 (57%), Gaps = 1/109 (0%)
Frame = +3
Query: 96 KRLIGRVDTDPVVHASKNL-PFLVQTLDIGVRPFIAALVNNVWRSTTPEEVLAMFLVELR 154
KRLIGR +D V + L PF V + + RP I N + T EE+ +M L+++R
Sbjct: 249 KRLIGRKFSDDSVESDMKLWPFKVISGPVD-RPMIVVNYKNEEKQFTAEEISSMVLIKMR 425
Query: 155 LMTETHLKRPIRNVVLTVPVSFSRFQLTRLERACAMAGLHVLRLMPEPT 203
+ E +L ++NVV+TVP F+ Q + A +AGL+V+R++ EPT
Sbjct: 426 EIAEAYLGSTVKNVVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPT 572
Score = 40.8 bits (94), Expect(2) = 1e-17
Identities = 23/71 (32%), Positives = 31/71 (43%)
Frame = +1
Query: 29 AIGIDIGTSQCSVAVWNGSEVELLKNKRNQKLMKSFVTFKDEAPSGGVTSQFSHEHEMLF 88
AIGID+GT+ V VW VE++ N + + S+V F D E L
Sbjct: 85 AIGIDLGTTYSCVGVWQNDRVEIIPNDQGNRTTPSYVAFTD--------------FERLI 222
Query: 89 GDTIFNMKRLI 99
GD N R +
Sbjct: 223 GDAAKNQMRSV 255
>TC9795 homologue to UP|Q9ATB8 (Q9ATB8) BiP-isoform D (Fragment), partial
(63%)
Length = 981
Score = 83.6 bits (205), Expect = 5e-17
Identities = 66/201 (32%), Positives = 104/201 (50%), Gaps = 6/201 (2%)
Frame = +1
Query: 19 EEKSSPLPEIAIGIDIGTSQCSVAVWNGSEVELLKNKRNQKLMKSFVTFKDEAPSGG--V 76
+E+ S L + IGID+GT+ V V+ VE++ N + ++ S+V F D G
Sbjct: 124 KEEPSKLGTV-IGIDLGTTYSCVGVYRNGHVEIIANDQGNRITPSWVAFNDGERLIGEAA 300
Query: 77 TSQFSHEHEMLFGDTIFNMKRLIGRVDTDPVVHASKNL-PFLVQTLDIGVRPFIAALVNN 135
+QF+ E TIF++KRLIGR D V L P+ + D +P+I + +
Sbjct: 301 KNQFAVNPER----TIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKD--GKPYIQVKIKD 462
Query: 136 -VWRSTTPEEVLAMFLVELRLMTETHLKRPIRNVVLTVPVSFSRFQLTRLERACAMAGLH 194
+ +PEEV AM L +++ E L + I + V+TVP F+ Q + A +AGL+
Sbjct: 463 GETKVFSPEEVSAMILTKMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDAGVIAGLN 642
Query: 195 VLRLMPEPTAV--ALLYGQQQ 213
V R++ EPT L GQ++
Sbjct: 643 VARIINEPTCCCHCLWIGQER 705
>TC14233 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (67%)
Length = 1570
Score = 82.0 bits (201), Expect = 2e-16
Identities = 48/148 (32%), Positives = 84/148 (56%), Gaps = 6/148 (4%)
Frame = +1
Query: 241 VAVTATAGGVSQIKALAGST-IGGEDLLQNMMRHLLPDSENIFKRHGVEEI----KSMAL 295
V++ G+ ++KA AG T +GGED M+ H + + FKR ++I +++
Sbjct: 1 VSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQE----FKRKNKKDISGNPRALRR 168
Query: 296 LRVATQDAIHQLSTQSSVQVDVD-LGNSLKICKVVDRAEFEEVNKEVFEKCESLIIQCLH 354
LR A + A LS+ + +++D L + + RA FEE+N ++F KC + +CL
Sbjct: 169 LRTACERAKRTLSSTAQTTIEIDSLYEGIDFYSPITRARFEEMNMDLFRKCMEPVEKCLR 348
Query: 355 DAKVEVGDINDVILVGGCSYIPQGWRIL 382
DAK++ ++DV+LVGG + IP+ ++L
Sbjct: 349 DAKMDKRSVHDVVLVGGSTRIPKVQQLL 432
>BP044919
Length = 519
Score = 73.9 bits (180), Expect = 4e-14
Identities = 52/168 (30%), Positives = 85/168 (49%), Gaps = 2/168 (1%)
Frame = +3
Query: 100 GRVDTDPVVHASKNL-PFLVQTLDIGVRPFIAALVNNVWRSTTPEEVLAMFLVELRLMTE 158
GR +D V+ L PF V +P I + PEE+ +M L +++ + E
Sbjct: 27 GRKYSDSVIQEDVKLWPFKV-IAGTDDKPMIVVKHKGEEKKLVPEEISSMVLSKMKEIAE 203
Query: 159 THLKRPIRNVVLTVPVSFSRFQLTRLERACAMAGLHVLRLMPEPTAVALLYGQQQQKASQ 218
L+ ++N V+TVP F+ Q + A +AGL+V+R++ EPTA AL YG Q++
Sbjct: 204 AFLESSVKNAVVTVPAYFNDAQRKATKDAGTIAGLNVMRIINEPTAAALAYGLQKR---- 371
Query: 219 ETMGSGSEKIALIFNMGAGYCDVAVTATAGGVSQIKALAGST-IGGED 265
E+ IF++G G DV++ ++KA +G T +G +D
Sbjct: 372 --ANLVGERNVFIFDLGGGTFDVSLLTIKDDNFEVKATSGDTHLGXKD 509
>TC14234 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (66%)
Length = 1528
Score = 72.8 bits (177), Expect = 9e-14
Identities = 42/130 (32%), Positives = 72/130 (55%), Gaps = 5/130 (3%)
Frame = +3
Query: 258 GSTIGGEDLLQNMMRHLLPDSENIFKRHGVEEI----KSMALLRVATQDAIHQLSTQSSV 313
G GGED M+ H + + FKR ++I +++ LR A + A LS+ +
Sbjct: 36 GHPSGGEDFDNRMVNHFVQE----FKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQT 203
Query: 314 QVDVD-LGNSLKICKVVDRAEFEEVNKEVFEKCESLIIQCLHDAKVEVGDINDVILVGGC 372
+++D L + + RA FEE+N ++F KC + +CL DAK++ ++DV+LVGG
Sbjct: 204 TIEIDSLYEGIDFYSPITRARFEELNMDLFRKCMEPVEKCLRDAKMDKKSVHDVVLVGGS 383
Query: 373 SYIPQGWRIL 382
+ IP+ ++L
Sbjct: 384 TRIPKVQQLL 413
>AW720237
Length = 459
Score = 68.2 bits (165), Expect = 2e-12
Identities = 46/151 (30%), Positives = 74/151 (48%), Gaps = 1/151 (0%)
Frame = +3
Query: 20 EKSSPLPEIAIGIDIGTSQCSVAVWNGSEVELLKNKRNQKLMKSFVTFKDEAPSGGVTSQ 79
E L AIGID+GT+ V VW VE++ N + + S+V F + G ++
Sbjct: 15 EPKQGLQATAIGIDLGTTYSCVGVWMNDRVEIIANDQGNRTTPSYVGFTESERLIGDAAK 194
Query: 80 FSHEHEMLFGDTIFNMKRLIGRVDTDPVVHASKNL-PFLVQTLDIGVRPFIAALVNNVWR 138
+ +T+F+ KRLIGR DP+V L PF V+ G +P IA +
Sbjct: 195 NQVARNPI--NTVFDAKRLIGRKFDDPIVQKDIKLWPFKVEAGPDG-KPLIAVKYKGETK 365
Query: 139 STTPEEVLAMFLVELRLMTETHLKRPIRNVV 169
E+V +M L +++ E +L + I+++V
Sbjct: 366 KFHAEQVSSMVLTKMKETAEAYLNKTIKDIV 458
>TC18168 homologue to UP|Q8GSN4 (Q8GSN4) Non-cell-autonomous heat shock
cognate protein 70, partial (23%)
Length = 508
Score = 67.8 bits (164), Expect = 3e-12
Identities = 47/145 (32%), Positives = 75/145 (51%), Gaps = 1/145 (0%)
Frame = +2
Query: 29 AIGIDIGTSQCSVAVWNGSEVELLKNKRNQKLMKSFVTFKDEAPSGGVTSQFSHEHEMLF 88
AIGID+GT+ V VW VE++ N + + S+V F D G ++ ++ M
Sbjct: 83 AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAK--NQVAMNP 256
Query: 89 GDTIFNMKRLIGRVDTDPVVHASKNL-PFLVQTLDIGVRPFIAALVNNVWRSTTPEEVLA 147
+T+F+ KRLIGR +D V + L PF V + +P I + EE+ +
Sbjct: 257 INTVFDAKRLIGRRVSDASVQSDMKLWPFKVNS-GPAEKPMIQVSYKGEDKQFAAEEISS 433
Query: 148 MFLVELRLMTETHLKRPIRNVVLTV 172
M L+++R + E +L I+N V+TV
Sbjct: 434 MVLMKMREIAEAYLGSTIKNAVVTV 508
>AV769455
Length = 556
Score = 65.5 bits (158), Expect = 1e-11
Identities = 44/122 (36%), Positives = 63/122 (51%), Gaps = 1/122 (0%)
Frame = +1
Query: 90 DTIFNMKRLIGRVDTDPVVHAS-KNLPFLVQTLDIGVRPFIAALVNNVWRSTTPEEVLAM 148
++I +KRLIGR DP + K+LPF V G P I A R+ TP +V M
Sbjct: 46 NSISQLKRLIGRQFADPELQRDLKSLPFAVTEGPDGF-PLIHARYLGETRTFTPTQVFGM 222
Query: 149 FLVELRLMTETHLKRPIRNVVLTVPVSFSRFQLTRLERACAMAGLHVLRLMPEPTAVALL 208
L L+ + + +L + + + +P+ F+ Q + A +AGLH LRL E TA AL
Sbjct: 223 MLSNLKEIAQKNLNAAVVDCCIGIPIYFTDLQRRAVLDAATIAGLHPLRLFHETTATALA 402
Query: 209 YG 210
YG
Sbjct: 403 YG 408
>BP064075
Length = 554
Score = 59.7 bits (143), Expect = 8e-10
Identities = 25/60 (41%), Positives = 45/60 (74%)
Frame = -2
Query: 329 VDRAEFEEVNKEVFEKCESLIIQCLHDAKVEVGDINDVILVGGCSYIPQGWRILLPTYAK 388
+ RA+FEE+NK+ F+KC L+ +C+ D+K++ +I+DV+LVGG S IP+ ++L+ + +
Sbjct: 505 ITRAKFEELNKDYFQKCMELVEKCVIDSKMDKSNIHDVVLVGGSSRIPKVRQLLMDFFGR 326
>AV409958
Length = 389
Score = 42.0 bits (97), Expect = 2e-04
Identities = 30/104 (28%), Positives = 54/104 (51%), Gaps = 9/104 (8%)
Frame = +2
Query: 282 FKRH-GVEEIKSMALLRVATQDAIH-QLSTQSSVQVDVDL-------GNSLKICKVVDRA 332
FKR+ G++ +K L+ T+ A ++ S Q ++ L I + RA
Sbjct: 29 FKRNEGIDLLKDKQALQRLTETAEKAKMELSSLTQTNISLPFITATADGPKHIETTLTRA 208
Query: 333 EFEEVNKEVFEKCESLIIQCLHDAKVEVGDINDVILVGGCSYIP 376
+FEE+ ++ ++ + + L DAK+ D+++VILVGG + IP
Sbjct: 209 KFEELCSDLLDRLRTPVENSLRDAKLSFKDLDEVILVGGSTRIP 340
>BP083571
Length = 423
Score = 37.4 bits (85), Expect = 0.004
Identities = 18/43 (41%), Positives = 25/43 (57%), Gaps = 2/43 (4%)
Frame = +1
Query: 29 AIGIDIGTSQCSVAVW--NGSEVELLKNKRNQKLMKSFVTFKD 69
AIGID+GT+ VAVW E++ N++ + SFV F D
Sbjct: 169 AIGIDLGTTYSCVAVWQEQNDRAEIIHNEQGNRTTPSFVAFTD 297
>BP062486
Length = 561
Score = 28.5 bits (62), Expect = 2.0
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +1
Query: 35 GTSQCSVAVWNGSEVELLKNKRNQKLMKSFVTFK 68
G QC V+ ++ LLKN+R+ + K VTF+
Sbjct: 13 GMCQCHVSFQTPHKLLLLKNRRSDQCCKQHVTFR 114
>AV766443
Length = 391
Score = 28.1 bits (61), Expect = 2.6
Identities = 15/47 (31%), Positives = 24/47 (50%), Gaps = 5/47 (10%)
Frame = +1
Query: 296 LRVATQDAIHQLSTQSSVQVDVDLGNSLKICKVVDRA-----EFEEV 337
+RVA L T S+Q+D+ + +S + ++ RA E EEV
Sbjct: 28 IRVADSTITFSLHTYKSIQIDIPINSSTSLLYIIKRA*LK*LEVEEV 168
>AV769359
Length = 524
Score = 28.1 bits (61), Expect = 2.6
Identities = 18/54 (33%), Positives = 24/54 (44%), Gaps = 12/54 (22%)
Frame = -2
Query: 64 FVTFKDEAPSGGVTSQF--SHEHEMLFGDTIF----------NMKRLIGRVDTD 105
F+ F++E GV F SH H LFG T F +K+ R+D D
Sbjct: 439 FLGFQNEKKGAGVRHNFCSSHRH*SLFGSTFFYSESILNPRSYLKKFFPRIDFD 278
>TC16989 similar to PIR|S66350|S66350 lycopene beta-cyclase - tomato
{Lycopersicon esculentum;} , partial (31%)
Length = 591
Score = 27.3 bits (59), Expect = 4.5
Identities = 14/36 (38%), Positives = 21/36 (57%)
Frame = +1
Query: 247 AGGVSQIKALAGSTIGGEDLLQNMMRHLLPDSENIF 282
A S+I+ +A T+ L NM+ HL+PD E +F
Sbjct: 376 ASNTSRIEIMAKGTVP----LVNMINHLIPDKE*VF 471
>CB827317
Length = 506
Score = 27.3 bits (59), Expect = 4.5
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +3
Query: 360 VGDINDVILVGGCSYIPQGWRILLP 384
VG+ ++ L GGC Y GW +P
Sbjct: 369 VGNFRELFLCGGCCYHKVGWGCKIP 443
>BG662417
Length = 484
Score = 26.6 bits (57), Expect = 7.7
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +2
Query: 323 LKICKVVDRAEFEEVNKEVFEKCESLIIQCLHDAK 357
L +C+ D AE EE+ KE++ + + QC D K
Sbjct: 329 LPLCRSNDIAEREEIIKEIWARFRVIEEQCSGDQK 433
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.134 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,687,161
Number of Sequences: 28460
Number of extensions: 66984
Number of successful extensions: 341
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 335
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 335
length of query: 394
length of database: 4,897,600
effective HSP length: 92
effective length of query: 302
effective length of database: 2,279,280
effective search space: 688342560
effective search space used: 688342560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC145222.11