
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145061.27 - phase: 0 /pseudo
(615 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF177539 38 0.005
BP042176 29 1.9
TC7899 UP|Q9FUF4 (Q9FUF4) Isoflavone reductase (Fragment), parti... 27 7.4
TC7898 similar to PIR|S17744|S17744 2''-hydroxyisoflavone reduct... 27 7.4
TC14451 homologue to GB|AAG40371.1|11762186|AF325019 AT4g27960 {... 27 7.4
TC17073 similar to UP|BAC98948 (BAC98948) AUX1-like auxin influx... 27 9.6
TC14794 homologue to UP|Q43312 (Q43312) Histone H2A, complete 27 9.6
>BF177539
Length = 327
Score = 37.7 bits (86), Expect = 0.005
Identities = 28/95 (29%), Positives = 40/95 (41%), Gaps = 1/95 (1%)
Frame = +3
Query: 45 LEGGIRCSCVKCTCRLIRSPKDVLNHLKDLGFMENYYVWIYHGEQEPTNNTEFDVNMHAS 104
++G I C C C R + V HL F NY W HGE T+ E + N A+
Sbjct: 30 VDGAILCPCGLCNFRKWLTRDVVYEHLIIKQFPRNYTFWFDHGE---THEVEIENNCTAN 200
Query: 105 SSEARMECEN-FGVMEDMVGDAVGVNLSYNEGGEE 138
S N + +MV +A GV++ + E
Sbjct: 201 VSSLGPNIVNDEDPIRNMVNEAFGVDMHRDNDSNE 305
>BP042176
Length = 474
Score = 29.3 bits (64), Expect = 1.9
Identities = 14/38 (36%), Positives = 17/38 (43%)
Frame = +1
Query: 471 HHGRKHS*FDCHRPFLPATHEFRKKKNLFTKGKTEKDG 508
HH R+ S H P P HE KN+ + EK G
Sbjct: 79 HHDRRWSPLQQHHPRRPRRHESXADKNILWRNCMEKTG 192
>TC7899 UP|Q9FUF4 (Q9FUF4) Isoflavone reductase (Fragment), partial (98%)
Length = 616
Score = 27.3 bits (59), Expect = 7.4
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +3
Query: 65 KDVLNHLKDLGFMENYYVWIYHGEQ 89
+ VL +K+ GF NY + +YH +Q
Sbjct: 195 EQVLKDIKESGFPHNYLLALYHSQQ 269
>TC7898 similar to PIR|S17744|S17744 2''-hydroxyisoflavone reductase -
alfalfa {Medicago sativa;} , partial (98%)
Length = 1174
Score = 27.3 bits (59), Expect = 7.4
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +1
Query: 65 KDVLNHLKDLGFMENYYVWIYHGEQ 89
+ VL +K+ GF NY + +YH +Q
Sbjct: 853 EQVLKDIKESGFPHNYLLALYHSQQ 927
>TC14451 homologue to GB|AAG40371.1|11762186|AF325019 AT4g27960 {Arabidopsis
thaliana;} , partial (84%)
Length = 997
Score = 27.3 bits (59), Expect = 7.4
Identities = 13/44 (29%), Positives = 19/44 (42%), Gaps = 2/44 (4%)
Frame = -2
Query: 51 CSCVKCTCRLIRSPKD--VLNHLKDLGFMENYYVWIYHGEQEPT 92
CSC+ CTC + P+D L L + W+ G P+
Sbjct: 669 CSCLSCTCGRFQGPRDHQGLGPLARNKLRATPWKWLVQGSTAPS 538
>TC17073 similar to UP|BAC98948 (BAC98948) AUX1-like auxin influx carrier
protein, partial (18%)
Length = 579
Score = 26.9 bits (58), Expect = 9.6
Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 4/25 (16%)
Frame = -3
Query: 360 YSCWPILLT----PYNVPLEMCMSK 380
+SCWPIL T P + P MC+S+
Sbjct: 169 WSCWPILQTQIQPPMSTPQ*MCLSQ 95
>TC14794 homologue to UP|Q43312 (Q43312) Histone H2A, complete
Length = 826
Score = 26.9 bits (58), Expect = 9.6
Identities = 17/60 (28%), Positives = 29/60 (48%)
Frame = -3
Query: 515 SEEVYRRVNELGLLKFTDVGKRVRHPKYGNEHHWTKRSIFWDLPYWKDNLLRHNFDVMHI 574
S +R + LG + DVG H N H K + F+ +P+ + ++ RHN ++ I
Sbjct: 488 SSSGFRPTSLLGEQEVVDVG---HHTAISNSHISQKLAQFFIIPHCQLDMARHNSSLLVI 318
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.138 0.450
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,986,279
Number of Sequences: 28460
Number of extensions: 211350
Number of successful extensions: 936
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 931
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 935
length of query: 615
length of database: 4,897,600
effective HSP length: 96
effective length of query: 519
effective length of database: 2,165,440
effective search space: 1123863360
effective search space used: 1123863360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC145061.27