Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC145061.27 - phase: 0 /pseudo
         (615 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BF177539                                                               38  0.005
BP042176                                                               29  1.9
TC7899 UP|Q9FUF4 (Q9FUF4) Isoflavone reductase (Fragment), parti...    27  7.4
TC7898 similar to PIR|S17744|S17744 2''-hydroxyisoflavone reduct...    27  7.4
TC14451 homologue to GB|AAG40371.1|11762186|AF325019 AT4g27960 {...    27  7.4
TC17073 similar to UP|BAC98948 (BAC98948) AUX1-like auxin influx...    27  9.6
TC14794 homologue to UP|Q43312 (Q43312) Histone H2A, complete          27  9.6

>BF177539 
          Length = 327

 Score = 37.7 bits (86), Expect = 0.005
 Identities = 28/95 (29%), Positives = 40/95 (41%), Gaps = 1/95 (1%)
 Frame = +3

Query: 45  LEGGIRCSCVKCTCRLIRSPKDVLNHLKDLGFMENYYVWIYHGEQEPTNNTEFDVNMHAS 104
           ++G I C C  C  R   +   V  HL    F  NY  W  HGE   T+  E + N  A+
Sbjct: 30  VDGAILCPCGLCNFRKWLTRDVVYEHLIIKQFPRNYTFWFDHGE---THEVEIENNCTAN 200

Query: 105 SSEARMECEN-FGVMEDMVGDAVGVNLSYNEGGEE 138
            S       N    + +MV +A GV++  +    E
Sbjct: 201 VSSLGPNIVNDEDPIRNMVNEAFGVDMHRDNDSNE 305


>BP042176 
          Length = 474

 Score = 29.3 bits (64), Expect = 1.9
 Identities = 14/38 (36%), Positives = 17/38 (43%)
 Frame = +1

Query: 471 HHGRKHS*FDCHRPFLPATHEFRKKKNLFTKGKTEKDG 508
           HH R+ S    H P  P  HE    KN+  +   EK G
Sbjct: 79  HHDRRWSPLQQHHPRRPRRHESXADKNILWRNCMEKTG 192


>TC7899 UP|Q9FUF4 (Q9FUF4) Isoflavone reductase (Fragment), partial (98%)
          Length = 616

 Score = 27.3 bits (59), Expect = 7.4
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = +3

Query: 65  KDVLNHLKDLGFMENYYVWIYHGEQ 89
           + VL  +K+ GF  NY + +YH +Q
Sbjct: 195 EQVLKDIKESGFPHNYLLALYHSQQ 269


>TC7898 similar to PIR|S17744|S17744 2''-hydroxyisoflavone reductase  -
           alfalfa {Medicago sativa;} , partial (98%)
          Length = 1174

 Score = 27.3 bits (59), Expect = 7.4
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = +1

Query: 65  KDVLNHLKDLGFMENYYVWIYHGEQ 89
           + VL  +K+ GF  NY + +YH +Q
Sbjct: 853 EQVLKDIKESGFPHNYLLALYHSQQ 927


>TC14451 homologue to GB|AAG40371.1|11762186|AF325019 AT4g27960 {Arabidopsis
           thaliana;} , partial (84%)
          Length = 997

 Score = 27.3 bits (59), Expect = 7.4
 Identities = 13/44 (29%), Positives = 19/44 (42%), Gaps = 2/44 (4%)
 Frame = -2

Query: 51  CSCVKCTCRLIRSPKD--VLNHLKDLGFMENYYVWIYHGEQEPT 92
           CSC+ CTC   + P+D   L  L         + W+  G   P+
Sbjct: 669 CSCLSCTCGRFQGPRDHQGLGPLARNKLRATPWKWLVQGSTAPS 538


>TC17073 similar to UP|BAC98948 (BAC98948) AUX1-like auxin influx carrier
           protein, partial (18%)
          Length = 579

 Score = 26.9 bits (58), Expect = 9.6
 Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 4/25 (16%)
 Frame = -3

Query: 360 YSCWPILLT----PYNVPLEMCMSK 380
           +SCWPIL T    P + P  MC+S+
Sbjct: 169 WSCWPILQTQIQPPMSTPQ*MCLSQ 95


>TC14794 homologue to UP|Q43312 (Q43312) Histone H2A, complete
          Length = 826

 Score = 26.9 bits (58), Expect = 9.6
 Identities = 17/60 (28%), Positives = 29/60 (48%)
 Frame = -3

Query: 515 SEEVYRRVNELGLLKFTDVGKRVRHPKYGNEHHWTKRSIFWDLPYWKDNLLRHNFDVMHI 574
           S   +R  + LG  +  DVG    H    N H   K + F+ +P+ + ++ RHN  ++ I
Sbjct: 488 SSSGFRPTSLLGEQEVVDVG---HHTAISNSHISQKLAQFFIIPHCQLDMARHNSSLLVI 318


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.324    0.138    0.450 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,986,279
Number of Sequences: 28460
Number of extensions: 211350
Number of successful extensions: 936
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 931
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 935
length of query: 615
length of database: 4,897,600
effective HSP length: 96
effective length of query: 519
effective length of database: 2,165,440
effective search space: 1123863360
effective search space used: 1123863360
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 58 (26.9 bits)


Medicago: description of AC145061.27