Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144807.5 + phase: 0 
         (112 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

AV418585                                                               27  0.38
TC17295 similar to UP|Q94JT2 (Q94JT2) AT5g17630/K10A8_110, parti...    26  0.84
TC8011 similar to UP|TPIC_FRAAN (Q9M4S8) Triosephosphate isomera...    26  0.84
TC16716 similar to GB|AAP37657.1|30725270|BT008298 At5g05630 {Ar...    25  1.4
TC13009 similar to GB|AAL09709.1|15982737|AY057475 AT4g23750/F9D...    24  3.2
BP033823                                                               24  4.2
TC7971 homologue to PIR|C86216|C86216 protein T23G18.6 [imported...    23  5.4
BU494350                                                               23  7.1
AV774902                                                               23  7.1
TC14768 similar to UP|Q7XXR8 (Q7XXR8) Nascent polypeptide associ...    23  9.3
TC19986                                                                23  9.3

>AV418585 
          Length = 414

 Score = 27.3 bits (59), Expect = 0.38
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -2

Query: 49  EKDSLTKTNKSIRNTLWDWK 68
           E+D++ KTNK +   LW W+
Sbjct: 278 EEDTVMKTNKQVYRCLWYWR 219


>TC17295 similar to UP|Q94JT2 (Q94JT2) AT5g17630/K10A8_110, partial (28%)
          Length = 566

 Score = 26.2 bits (56), Expect = 0.84
 Identities = 16/38 (42%), Positives = 20/38 (52%), Gaps = 3/38 (7%)
 Frame = +3

Query: 24  GLGAVFAILGARSYNQ---QKIIEALEAEKDSLTKTNK 58
           GLG+  AILG   Y+Q    K  + +E EK S  K  K
Sbjct: 297 GLGSAIAILGTFLYSQATSAKTAKKIEGEKSS*RKRRK 410


>TC8011 similar to UP|TPIC_FRAAN (Q9M4S8) Triosephosphate isomerase,
           chloroplast precursor  (TIM) , partial (89%)
          Length = 1305

 Score = 26.2 bits (56), Expect = 0.84
 Identities = 14/31 (45%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
 Frame = +2

Query: 81  PVPLARLYEIYGEAAP-PQQSAPGNLFFSLL 110
           P+P AR  +      P P QS+P NLFF  L
Sbjct: 137 PIPQARFSQFSIHPFPLPFQSSPSNLFFQTL 229


>TC16716 similar to GB|AAP37657.1|30725270|BT008298 At5g05630 {Arabidopsis
           thaliana;}, partial (28%)
          Length = 580

 Score = 25.4 bits (54), Expect = 1.4
 Identities = 10/28 (35%), Positives = 18/28 (63%)
 Frame = -3

Query: 43  IEALEAEKDSLTKTNKSIRNTLWDWKQQ 70
           I  +  E  +   +N+S+RNT ++W+QQ
Sbjct: 368 ITTIFREHGTHLSSNQSLRNTPYEWEQQ 285


>TC13009 similar to GB|AAL09709.1|15982737|AY057475 AT4g23750/F9D16_220
           {Arabidopsis thaliana;}, partial (5%)
          Length = 642

 Score = 24.3 bits (51), Expect = 3.2
 Identities = 11/30 (36%), Positives = 20/30 (66%)
 Frame = -3

Query: 40  QKIIEALEAEKDSLTKTNKSIRNTLWDWKQ 69
           Q IIEA+ + ++ L K +  + + +W+WKQ
Sbjct: 235 QHIIEAVVSVQNIL-KLDVKLHHIIWNWKQ 149


>BP033823 
          Length = 529

 Score = 23.9 bits (50), Expect = 4.2
 Identities = 11/27 (40%), Positives = 14/27 (51%)
 Frame = -3

Query: 86  RLYEIYGEAAPPQQSAPGNLFFSLLLI 112
           RLY  +G   PP    P  LF  LL++
Sbjct: 473 RLYRFFGRLFPPSFE*PFQLFRVLLIL 393


>TC7971 homologue to PIR|C86216|C86216 protein T23G18.6 [imported] -
            Arabidopsis thaliana {Arabidopsis thaliana;}, partial
            (98%)
          Length = 1662

 Score = 23.5 bits (49), Expect = 5.4
 Identities = 12/41 (29%), Positives = 21/41 (50%), Gaps = 7/41 (17%)
 Frame = +3

Query: 61   RNTLWD-------WKQQLYAEAASDSAPVPLARLYEIYGEA 94
            + +LWD       ++ + YAEA   +   P+A    +YG+A
Sbjct: 1110 KTSLWDLLESTLTYQHRTYAEAVKKAIAKPIAS*GGLYGDA 1232


>BU494350 
          Length = 537

 Score = 23.1 bits (48), Expect = 7.1
 Identities = 10/15 (66%), Positives = 12/15 (79%)
 Frame = +3

Query: 71  LYAEAASDSAPVPLA 85
           L +E+ASDS PVP A
Sbjct: 459 LKSESASDSTPVPAA 503


>AV774902 
          Length = 594

 Score = 23.1 bits (48), Expect = 7.1
 Identities = 7/10 (70%), Positives = 8/10 (80%)
 Frame = +3

Query: 64  LWDWKQQLYA 73
           LWDW  QLY+
Sbjct: 453 LWDWASQLYS 482


>TC14768 similar to UP|Q7XXR8 (Q7XXR8) Nascent polypeptide associated
           complex alpha chain, partial (21%)
          Length = 1036

 Score = 22.7 bits (47), Expect = 9.3
 Identities = 13/32 (40%), Positives = 16/32 (49%), Gaps = 3/32 (9%)
 Frame = +1

Query: 59  SIRNTLWDWKQQL---YAEAASDSAPVPLARL 87
           SIRN  W W Q L    + +   S P+ L RL
Sbjct: 169 SIRNEAWIWSQWLGHPLSCSIGKSRPILLCRL 264


>TC19986 
          Length = 531

 Score = 22.7 bits (47), Expect = 9.3
 Identities = 9/25 (36%), Positives = 14/25 (56%)
 Frame = -2

Query: 60 IRNTLWDWKQQLYAEAASDSAPVPL 84
          +RN  W WK+ L  E+ S +  + L
Sbjct: 98 VRNNSWVWKRLLRKESFSKAEGIKL 24


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.316    0.131    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,605,589
Number of Sequences: 28460
Number of extensions: 14808
Number of successful extensions: 87
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 87
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87
length of query: 112
length of database: 4,897,600
effective HSP length: 88
effective length of query: 24
effective length of database: 2,393,120
effective search space: 57434880
effective search space used: 57434880
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)


Medicago: description of AC144807.5