BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144807.2 + phase: 0
(239 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9779 similar to UP|Q93Z44 (Q93Z44) F25I18.1/F25I18.1 (Expresse... 256 2e-69
TC8900 35 0.012
TC8587 similar to UP|Q84MD8 (Q84MD8) At4g21470, partial (97%) 33 0.034
TC16150 similar to GB|AAO63328.1|28950809|BT005264 At4g25840 {Ar... 32 0.13
TC8676 similar to UP|O48587 (O48587) DREG-2 like protein, partia... 31 0.22
TC8835 30 0.49
BP078304 27 2.4
TC7879 weakly similar to GB|AAM16239.1|20334756|AY093978 At2g321... 27 2.4
AV418517 27 3.2
TC8579 similar to UP|Q945P3 (Q945P3) At1g23740/F5O8_27, partial ... 27 3.2
BP044083 27 4.1
TC14015 weakly similar to UP|Q9ASR9 (Q9ASR9) At2g01320/F10A8.20,... 26 5.4
TC14462 weakly similar to GB|BAA97483.1|8885553|AB025604 ripenin... 26 5.4
>TC9779 similar to UP|Q93Z44 (Q93Z44) F25I18.1/F25I18.1 (Expressed
protein), partial (62%)
Length = 1040
Score = 256 bits (654), Expect = 2e-69
Identities = 137/220 (62%), Positives = 152/220 (68%)
Frame = +1
Query: 19 SHLTPKTRLRGVVFDMDGTLTVPVIDFPSMYKAVLGDDEYLRVKASNPVGIDILKLIDHW 78
S T KT LRGVVFDMDGTLT+PVIDFP+MY+AVLGDDEY R+KA +P GIDIL ID W
Sbjct: 40 STTTTKTPLRGVVFDMDGTLTIPVIDFPAMYRAVLGDDEYRRIKAQSPSGIDILSHIDRW 219
Query: 79 SPLQQRQAYDTIAHFEKQALDHLQIMPGAADLCNVLDSKKIRRGLITRNMKSAVDLFHQR 138
QQ+QAYDTIA FE+QAL HLQIMPGAADLC +LDSK+IRRGLITRN+KSAVD+FH+R
Sbjct: 220 PQHQQQQAYDTIADFERQALLHLQIMPGAADLCRLLDSKQIRRGLITRNVKSAVDIFHER 399
Query: 139 FGITFSPALSREFRPYKPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCL 198
FG + CG +AGA TCL
Sbjct: 400 FG---------------------------------------------VVCGRQAGAFTCL 444
Query: 199 LDQTGRYDSPEYANVDFKPDFKVTSLAEVYSILETNFELS 238
LDQTGRYDS YANV FKPDFKVTSLAEVYSILE NF+LS
Sbjct: 445 LDQTGRYDSFPYANVGFKPDFKVTSLAEVYSILEANFDLS 564
>TC8900
Length = 596
Score = 35.0 bits (79), Expect = 0.012
Identities = 19/53 (35%), Positives = 26/53 (48%)
Frame = +1
Query: 146 ALSREFRPYKPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCL 198
A+S E KP+P L C L V+P + + VGD ++DI AG L
Sbjct: 175 AVSAEVAAEKPNPTIFLKACELLGVKPEDAVHVGDDRRNDIWGARDAGCDAWL 333
>TC8587 similar to UP|Q84MD8 (Q84MD8) At4g21470, partial (97%)
Length = 1519
Score = 33.5 bits (75), Expect = 0.034
Identities = 36/191 (18%), Positives = 76/191 (38%), Gaps = 11/191 (5%)
Frame = +2
Query: 3 LLSSTKAFLLFPRIMSSHLTPKTRLRGVVFDMDGTLTVPVIDFPSMYKAVLGD------- 55
+L STK ++ ++ + V+ D+DGTL ++ K +LG+
Sbjct: 47 ILQSTKLKFTNEKMSIANPLSNKLVTCVILDLDGTLLNTDGIVGNVLKVLLGNYGKEWDG 226
Query: 56 DEYLRVKASNPVGIDILKLIDHWSPLQQRQAYDTIAHFEKQALDHLQIMPGAADLCNVLD 115
E ++ P + D+ + + I+ +++ +PGA L L
Sbjct: 227 REANKIVGKTPFEASAAVIEDYGLTCSTAEFFSQISPLFADQWCNIKALPGANRLIKHLK 406
Query: 116 SKKIRRGLITRNMKSAVDL---FHQRFGITFSPALSR-EFRPYKPDPAPLLHICSLWEVQ 171
S + L + + + ++ FH + +F+ + E R KP P + ++
Sbjct: 407 SHAVPMALASNSSRENIEAKISFHDGWKDSFAVIIGGDEVRTGKPSPDIFIEAARRLGIE 586
Query: 172 PNEVIMVGDSL 182
P+ +++ DSL
Sbjct: 587 PSNCLVIEDSL 619
>TC16150 similar to GB|AAO63328.1|28950809|BT005264 At4g25840 {Arabidopsis
thaliana;}, partial (90%)
Length = 1065
Score = 31.6 bits (70), Expect = 0.13
Identities = 41/179 (22%), Positives = 72/179 (39%), Gaps = 18/179 (10%)
Frame = +2
Query: 21 LTPKTRLRGVVFDMDGTLTVPVIDFPSMYKAVLGD-----DEYLRVKASNPVGIDILKLI 75
++ +T + VVFDMDG L + + + +L D L+ K I+ ++
Sbjct: 164 VSARTPITHVVFDMDGLLLDTEKFYTEVQELILARYNKTFDWSLKAKMMGNKAIEAARVF 343
Query: 76 DHWSPLQQRQAYDTIAHFEKQALDHL----QIMPGAADLCNVLDSKKIRRGLITRNMKSA 131
+ + + + + L L +MPGA+ L L +K + L T +++
Sbjct: 344 VEETGISDSLSAEQFLVEREDTLLKLFPTSDLMPGASRLLKHLHAKGVPIALATGSLRKH 523
Query: 132 VDLFHQRFGITFS------PALSREFRPYKPDPAPLLHICSLWE---VQPNEVIMVGDS 181
+L QR G FS E + KP P L +E V P +V++ D+
Sbjct: 524 FELKTQRHGELFSLMHHVVVGDDPEVKHGKPSPDVFLAAAKRFEGGPVDPCKVLVFEDA 700
>TC8676 similar to UP|O48587 (O48587) DREG-2 like protein, partial (95%)
Length = 1346
Score = 30.8 bits (68), Expect = 0.22
Identities = 16/55 (29%), Positives = 27/55 (49%), Gaps = 2/55 (3%)
Frame = +2
Query: 170 VQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQTGRYDSPEY--ANVDFKPDFKVT 222
++P E + +GDS++ D G H LLD+ D+ E+ + PD + T
Sbjct: 839 IKPEETLHIGDSMRKDYEPAKSIGMHALLLDRFKTPDADEWRKSGAVVLPDLEAT 1003
>TC8835
Length = 596
Score = 29.6 bits (65), Expect = 0.49
Identities = 17/65 (26%), Positives = 30/65 (46%)
Frame = +1
Query: 155 KPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQTGRYDSPEYANVD 214
KPDPA + + V+P+ ++V DS +A C++ ++G ++ N D
Sbjct: 127 KPDPAIYILAANTLGVEPSRCVVVEDSAIG--LAAAKAAGMKCIITKSGYTADEDFLNAD 300
Query: 215 FKPDF 219
DF
Sbjct: 301 AVFDF 315
>BP078304
Length = 441
Score = 27.3 bits (59), Expect = 2.4
Identities = 19/53 (35%), Positives = 26/53 (48%), Gaps = 1/53 (1%)
Frame = -1
Query: 113 VLDSKKIRRGLITRNMKSAVDLFHQRFGITFSPALSREFRPYKP-DPAPLLHI 164
+LD + G I+ AVD+FH FG+ L E+ KP DPA L +
Sbjct: 258 ILDCQDTENGGISDRPDDAVDVFHTYFGVAGLSLL--EYPGLKPIDPAYALPV 106
>TC7879 weakly similar to GB|AAM16239.1|20334756|AY093978
At2g32150/F22D22.10 {Arabidopsis thaliana;}, partial
(92%)
Length = 1344
Score = 27.3 bits (59), Expect = 2.4
Identities = 54/236 (22%), Positives = 89/236 (36%), Gaps = 30/236 (12%)
Frame = +2
Query: 6 STKAFLLFPRIMSSHLTPKTRLRGVVFDMDGTLTVPVIDFPSMYKAVLGDDEYLRVKA-- 63
S +F L +M+ + ++FD+D TL + K + D +L K
Sbjct: 65 SLLSFFLIFSVMAFGTRAASPFDCIIFDLDDTLYPSTTGIGASVKKNI--DLFLIEKCGF 238
Query: 64 ----SNPVGIDILKLIDHWSPLQQRQAYDTIAH----FEKQALDHLQIMPGAADLCNVLD 115
++ + +++ K + YD A F L + QI P L N+L
Sbjct: 239 SPAKASSLRVELFKTYGSTLAGLRALGYDVSAEDYHSFVHGRLPYDQIKPDF-QLRNLLR 415
Query: 116 SKKIRRGLITRNMKSAVDLFHQRFGI-----------TFSPALSREFRPYKPDPAPLLHI 164
S + R+ + T + + R GI T +P L RP D P+L
Sbjct: 416 SIQQRKIIFTNSDRIHALRSLDRLGIQDCFEQIICFETMNPNLPHATRP---DEFPVLLK 586
Query: 165 CSL---------WEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQTGRYDSPEYA 211
SL V P + + DS+ + +A G G HT L+ +T + +YA
Sbjct: 587 PSLDAVKVAIHTANVDPRRTLFLDDSVHN-VAAGKEMGLHTVLVGKTVKCKGADYA 751
>AV418517
Length = 298
Score = 26.9 bits (58), Expect = 3.2
Identities = 14/38 (36%), Positives = 20/38 (51%)
Frame = -3
Query: 88 DTIAHFEKQALDHLQIMPGAADLCNVLDSKKIRRGLIT 125
+T HF K+ +D+L I+ D CN L S L+T
Sbjct: 254 NTCEHFRKRFVDYLNILQLFPDRCNFLLSSIHNHHLLT 141
>TC8579 similar to UP|Q945P3 (Q945P3) At1g23740/F5O8_27, partial (40%)
Length = 619
Score = 26.9 bits (58), Expect = 3.2
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = +3
Query: 213 VDFKPDFKVTSLAEVYSILETN 234
VD K F T LAE +S LETN
Sbjct: 363 VDSKGPFPFTQLAEAFSYLETN 428
>BP044083
Length = 574
Score = 26.6 bits (57), Expect = 4.1
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +1
Query: 153 PYKPDPAPLLHICSLWEVQPN 173
P+ P P P+L S W++ PN
Sbjct: 238 PFSPGPKPMLFSSSKWKLLPN 300
>TC14015 weakly similar to UP|Q9ASR9 (Q9ASR9) At2g01320/F10A8.20, partial
(6%)
Length = 360
Score = 26.2 bits (56), Expect = 5.4
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = -1
Query: 188 CGGRAGAHTCLLDQTGR 204
CGG GAH QTGR
Sbjct: 279 CGGATGAHAARASQTGR 229
>TC14462 weakly similar to GB|BAA97483.1|8885553|AB025604 ripening-related
protein-like; hydrolase-like {Arabidopsis thaliana;} ,
partial (80%)
Length = 1072
Score = 26.2 bits (56), Expect = 5.4
Identities = 15/57 (26%), Positives = 25/57 (43%)
Frame = +1
Query: 155 KPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQTGRYDSPEYA 211
KP + + + P + DS+++ I G R G HT L+ + R +YA
Sbjct: 751 KPQEHAIELALKIANLNPQRTLFFEDSVRN-IQAGKRVGLHTVLVGTSQRVKGADYA 918
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.139 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,061,719
Number of Sequences: 28460
Number of extensions: 56764
Number of successful extensions: 253
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 251
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 253
length of query: 239
length of database: 4,897,600
effective HSP length: 87
effective length of query: 152
effective length of database: 2,421,580
effective search space: 368080160
effective search space used: 368080160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144807.2