BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144807.11 - phase: 0 /pseudo
(303 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19031 similar to GB|AAR24233.1|38566686|BT010866 At1g04520 {Ar... 186 5e-48
TC12257 weakly similar to UP|Q9FHQ4 (Q9FHQ4) Similarity to secre... 82 1e-16
TC12940 73 5e-14
TC19386 similar to GB|AAR24233.1|38566686|BT010866 At1g04520 {Ar... 69 1e-12
AV767962 64 2e-11
TC14519 62 1e-10
TC12338 similar to GB|AAP13422.1|30023778|BT006314 At2g01660 {Ar... 58 2e-09
AW720134 52 1e-07
BP040765 49 8e-07
AV766715 34 0.035
BP081081 31 0.23
BF177869 31 0.23
TC7910 homologue to UP|P93166 (P93166) SCOF-1, partial (52%) 31 0.23
TC19245 similar to UP|Q99K50 (Q99K50) Nucleolin, partial (3%) 30 0.66
BP054670 30 0.66
TC14229 similar to UP|P93166 (P93166) SCOF-1, partial (38%) 29 0.86
BP085186 29 1.1
TC15720 similar to UP|O35328 (O35328) Proline-rich protein 9-1 (... 29 1.1
BP049917 28 1.5
TC19515 similar to UP|Q9YGK0 (Q9YGK0) Vitellogenin precursor, pa... 28 1.9
>TC19031 similar to GB|AAR24233.1|38566686|BT010866 At1g04520 {Arabidopsis
thaliana;} , partial (30%)
Length = 637
Score = 186 bits (471), Expect = 5e-48
Identities = 91/126 (72%), Positives = 108/126 (85%), Gaps = 4/126 (3%)
Frame = +3
Query: 1 MGFPKKPLFLLLFFIFFTTHFSK----SSSSPDYTTLVYKGCSKETFTDPNGAYSQSLSA 56
MGFP+ PLFL LF +F T+ + S+ YT+LVYKGCSK+TF+DPNG YSQ+LSA
Sbjct: 261 MGFPRNPLFLSLF-LFLLTNLGLLPQLAESAAAYTSLVYKGCSKDTFSDPNGVYSQALSA 437
Query: 57 LFGSLVSQSTKTRFFKTTTGNGQNSITGIFQCRGDLTNSDCYSCVSKLPVLSDKLCGKTI 116
LFGSLVSQSTK +FFK TTG+GQ+SITG+FQCRGDL+NSDCY+CVSKLPVLSDKLCGKT+
Sbjct: 438 LFGSLVSQSTKGKFFKATTGSGQSSITGLFQCRGDLSNSDCYNCVSKLPVLSDKLCGKTV 617
Query: 117 SARVQL 122
+ARVQL
Sbjct: 618 AARVQL 635
Score = 32.7 bits (73), Expect = 0.078
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +3
Query: 191 QCEGDVGDSDCGECVKSAVQRAQVECGSSISGQIYL 226
QC GD+ +SDC CV + CG +++ ++ L
Sbjct: 528 QCRGDLSNSDCYNCVSKLPVLSDKLCGKTVAARVQL 635
>TC12257 weakly similar to UP|Q9FHQ4 (Q9FHQ4) Similarity to secretory
protein, partial (49%)
Length = 475
Score = 82.0 bits (201), Expect = 1e-16
Identities = 42/137 (30%), Positives = 70/137 (50%)
Frame = +2
Query: 12 LFFIFFTTHFSKSSSSPDYTTLVYKGCSKETFTDPNGAYSQSLSALFGSLVSQSTKTRFF 71
L + F S SS +Y GC+++ +T PN Y +L++L SLV+ +T + +
Sbjct: 47 LSLLIFANLIVLSLSSSQLDLFLYGGCTQQRYT-PNSPYELNLNSLLTSLVNSATYSSYN 223
Query: 72 KTTTGNGQNSITGIFQCRGDLTNSDCYSCVSKLPVLSDKLCGKTISARVQLSGCYMLYEV 131
T + G++QCRGD++ DC +C+S+ + LC + VQL GCY+ Y+
Sbjct: 224 NYTVVGADDVAYGLYQCRGDISMPDCATCISRAVSRAGVLCQEARGGAVQLEGCYVKYDN 403
Query: 132 AGFPQVSGMQILFKTCG 148
A F ++ + CG
Sbjct: 404 AKFLGAEDKTVVLRKCG 454
Score = 43.5 bits (101), Expect = 4e-05
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = +2
Query: 187 YVMGQCEGDVGDSDCGECVKSAVQRAQVECGSSISGQIYLHKCFISF 233
Y + QC GD+ DC C+ AV RA V C + G + L C++ +
Sbjct: 257 YGLYQCRGDISMPDCATCISRAVSRAGVLCQEARGGAVQLEGCYVKY 397
>TC12940
Length = 480
Score = 73.2 bits (178), Expect = 5e-14
Identities = 35/81 (43%), Positives = 51/81 (62%)
Frame = +3
Query: 223 QIYLHKCFISFSYYPNGVPKGGHSSSGGGSSSSSYSSSFSDAMTGQNTGKTVAIILGGIA 282
Q+YL+ C+IS+S+YPNGVP SS GG Q+T +TVAI +GG+A
Sbjct: 3 QVYLYSCYISYSFYPNGVPTIASSSPGGH----------------QHTSRTVAIAVGGVA 134
Query: 283 AVAFLVICLLFARNLVKKRDG 303
A FL++CL+ ++++KKR G
Sbjct: 135 AFGFLIVCLMCLKSVLKKRSG 197
>TC19386 similar to GB|AAR24233.1|38566686|BT010866 At1g04520 {Arabidopsis
thaliana;} , partial (10%)
Length = 522
Score = 68.6 bits (166), Expect = 1e-12
Identities = 39/51 (76%), Positives = 41/51 (79%)
Frame = +3
Query: 252 SSSSSYSSSFSDAMTGQNTGKTVAIILGGIAAVAFLVICLLFARNLVKKRD 302
SSSSS SSSFS GQN GKTVAIILGG A VAFL+I LLFARNL+KK D
Sbjct: 3 SSSSSGSSSFS----GQNAGKTVAIILGGAAGVAFLIIMLLFARNLMKKHD 143
>AV767962
Length = 582
Score = 64.3 bits (155), Expect = 2e-11
Identities = 34/130 (26%), Positives = 59/130 (45%)
Frame = +1
Query: 5 KKPLFLLLFFIFFTTHFSKSSSSPDYTTLVYKGCSKETFTDPNGAYSQSLSALFGSLVSQ 64
KK + LL IF + S D + + + C++ T G S ++ L + +
Sbjct: 19 KKIMHLLYKIIFSLLLCISIAESQDLSGPLGEFCNENTKISSGGKLSANIDKLLAEFILK 198
Query: 65 STKTRFFKTTTGNGQNSITGIFQCRGDLTNSDCYSCVSKLPVLSDKLCGKTISARVQLSG 124
+ T F T+ GN Q+ + G+ QCRGD++ DC +C+ + C + AR+
Sbjct: 199 TPSTGFVATSYGNNQDQVYGLAQCRGDVSTQDCSNCIQDAAKQIRQKCPNQVDARIWYDY 378
Query: 125 CYMLYEVAGF 134
C++ Y F
Sbjct: 379 CFLRYNNKSF 408
Score = 47.8 bits (112), Expect = 2e-06
Identities = 18/62 (29%), Positives = 34/62 (54%), Gaps = 4/62 (6%)
Frame = +1
Query: 177 GFYVTTY----QSLYVMGQCEGDVGDSDCGECVKSAVQRAQVECGSSISGQIYLHKCFIS 232
GF T+Y +Y + QC GDV DC C++ A ++ + +C + + +I+ CF+
Sbjct: 211 GFVATSYGNNQDQVYGLAQCRGDVSTQDCSNCIQDAAKQIRQKCPNQVDARIWYDYCFLR 390
Query: 233 FS 234
++
Sbjct: 391 YN 396
>TC14519
Length = 1095
Score = 62.0 bits (149), Expect = 1e-10
Identities = 45/212 (21%), Positives = 88/212 (41%), Gaps = 12/212 (5%)
Frame = +2
Query: 38 CSKETFTDPNGAYSQSLSALFGSLVSQSTKTRFFKTTTGNGQNSITGIFQCRGDLTNSDC 97
CS + Y +L+AL L +++ F + G GQN G+ CRGDL ++DC
Sbjct: 116 CSMYEHYSSDDPYETNLNALLSHLKNETPNAGFVAASKGEGQNQTYGLALCRGDLASTDC 295
Query: 98 YSCVSKLPVLSDKLCGKTISARVQLSGCYMLYEV----AGFPQVSGMQILFKTCGTTNAA 153
SC+++ + C + C + Y +G ++ + FK+
Sbjct: 296 GSCIAQAIHDMFEDCPYNKDVMIWHEDCTVGYSKEHNNSGI-KIDDTMLCFKSQNDVEVP 472
Query: 154 GRGFEERRDTAF--SVMENGVVSNHGF-----YVTTYQSLYVMGQCEGDVGDSDCGECVK 206
++ F + + + ++ + + Y +Y QC D+ +C +C++
Sbjct: 473 NPKVFIKKTQEFLSQITQKEELGSNNYASGEVKIDEYNMVYGYAQCNRDLSSKECSDCLE 652
Query: 207 SAVQRAQVECGSSISG-QIYLHKCFISFSYYP 237
++ A+ ECG G ++Y C + + YP
Sbjct: 653 RSLDYAK-ECGRGKEGVRVYSGICRLRYDMYP 745
>TC12338 similar to GB|AAP13422.1|30023778|BT006314 At2g01660 {Arabidopsis
thaliana;}, partial (26%)
Length = 903
Score = 58.2 bits (139), Expect = 2e-09
Identities = 50/200 (25%), Positives = 78/200 (39%), Gaps = 10/200 (5%)
Frame = -1
Query: 13 FFIFFTTHFSKSSSSPDYT-----TLVYKGC--SKETFTDPNGAYSQSLSALFGSLVSQS 65
FF F ++ FS S S TL S T P Y +++L SL + +
Sbjct: 831 FFFFVSSSFSHSQPSQPLQLHHQ*TLSSSAVALSPRTRRFPTPIYETGVNSLLTSLFNSA 652
Query: 66 TKTRFFKTT---TGNGQNSITGIFQCRGDLTNSDCYSCVSKLPVLSDKLCGKTISARVQL 122
+ T + T T +++ G++QCRGDL N Y CV++ LC +QL
Sbjct: 651 SFTNYNNFTVPATAGSGDTLYGLYQCRGDLNNDQRYRCVARAVSQLGTLCSAVRGGALQL 472
Query: 123 SGCYMLYEVAGFPQVSGMQILFKTCGTTNAAGRGFEERRDTAFSVMENGVVSNHGFYVTT 182
GC + Y+ V ++ K C + RRD + Y+ T
Sbjct: 471 EGCPVKYDNVKLLGVEDKTMVVKKCRPSIGLTSDASTRRDAVLA------------YIQT 328
Query: 183 YQSLYVMGQCEGDVGDSDCG 202
+Y+ + + G S CG
Sbjct: 327 SDGVYMDVEDQRLWGFSGCG 268
Score = 41.6 bits (96), Expect = 2e-04
Identities = 27/114 (23%), Positives = 49/114 (42%)
Frame = -2
Query: 180 VTTYQSLYVMGQCEGDVGDSDCGECVKSAVQRAQVECGSSISGQIYLHKCFISFSYYPNG 239
++ Y C D+ S+C +C+ ++QR + ECG+S ++YL C+ +
Sbjct: 299 ISAYGDFQGAAHCTVDLTPSECQDCLSESIQRLKTECGASTWREMYLANCYAALLRGLGS 120
Query: 240 VPKGGHSSSGGGSSSSSYSSSFSDAMTGQNTGKTVAIILGGIAAVAFLVICLLF 293
+P ++ + KT+AI L IA VA +++ L F
Sbjct: 119 LPPQ*RYNNHNDN----------------EIDKTLAI*L*LIAEVALIIVSLSF 6
Score = 30.8 bits (68), Expect = 0.30
Identities = 14/49 (28%), Positives = 23/49 (46%)
Frame = -1
Query: 185 SLYVMGQCEGDVGDSDCGECVKSAVQRAQVECGSSISGQIYLHKCFISF 233
+LY + QC GD+ + CV AV + C + G + L C + +
Sbjct: 597 TLYGLYQCRGDLNNDQRYRCVARAVSQLGTLCSAVRGGALQLEGCPVKY 451
Score = 29.6 bits (65), Expect = 0.66
Identities = 14/43 (32%), Positives = 20/43 (45%)
Frame = -2
Query: 84 GIFQCRGDLTNSDCYSCVSKLPVLSDKLCGKTISARVQLSGCY 126
G C DLT S+C C+S+ CG + + L+ CY
Sbjct: 275 GAAHCTVDLTPSECQDCLSESIQRLKTECGASTWREMYLANCY 147
>AW720134
Length = 515
Score = 52.0 bits (123), Expect = 1e-07
Identities = 40/123 (32%), Positives = 54/123 (43%), Gaps = 2/123 (1%)
Frame = +3
Query: 14 FIFFTTHFSKSSSSPDYTTLVYKGCSKETFTDPNGAYSQSLSALFGSLVSQSTKTRFFKT 73
F FFTT ++ +P Y +YK CS T T N AY L L SL S +T+ F +
Sbjct: 3 FFFFTT---EAQDTPVY---LYKNCSTNT-TTTNSAYHLHLRTLLNSLSSNATE--FHNS 155
Query: 74 TTGNGQNSIT--GIFQCRGDLTNSDCYSCVSKLPVLSDKLCGKTISARVQLSGCYMLYEV 131
T S T G+F CRGD+ + C+ CV C + A + C + Y
Sbjct: 156 TVAGTSPSDTAYGLFMCRGDVDPAICHQCVVNATHRLQTECSLSNQAIIWYDECTVRYSN 335
Query: 132 AGF 134
F
Sbjct: 336 RSF 344
Score = 42.4 bits (98), Expect = 1e-04
Identities = 25/96 (26%), Positives = 40/96 (41%), Gaps = 4/96 (4%)
Frame = +3
Query: 143 LFKTCGT-TNAAGRGFEERRDTAFSVMENGVVSNHGFYV---TTYQSLYVMGQCEGDVGD 198
L+K C T T + T + + + H V + + Y + C GDV
Sbjct: 45 LYKNCSTNTTTTNSAYHLHLRTLLNSLSSNATEFHNSTVAGTSPSDTAYGLFMCRGDVDP 224
Query: 199 SDCGECVKSAVQRAQVECGSSISGQIYLHKCFISFS 234
+ C +CV +A R Q EC S I+ +C + +S
Sbjct: 225 AICHQCVVNATHRLQTECSLSNQAIIWYDECTVRYS 332
>BP040765
Length = 424
Score = 49.3 bits (116), Expect = 8e-07
Identities = 22/60 (36%), Positives = 33/60 (54%)
Frame = -2
Query: 189 MGQCEGDVGDSDCGECVKSAVQRAQVECGSSISGQIYLHKCFISFSYYPNGVPKGGHSSS 248
M QC GD+ +C +CV A+ R + +CG++ G ++L KCF +S G H SS
Sbjct: 357 MAQCTGDLSGGECQDCVSEAISRLKGDCGTADYGDMFLGKCFARYSV------GGAHDSS 196
Score = 38.1 bits (87), Expect = 0.002
Identities = 18/56 (32%), Positives = 25/56 (44%)
Frame = -2
Query: 78 GQNSITGIFQCRGDLTNSDCYSCVSKLPVLSDKLCGKTISARVQLSGCYMLYEVAG 133
G + G+ QC GDL+ +C CVS+ CG + L C+ Y V G
Sbjct: 378 GSGGVRGMAQCTGDLSGGECQDCVSEAISRLKGDCGTADYGDMFLGKCFARYSVGG 211
>AV766715
Length = 468
Score = 33.9 bits (76), Expect = 0.035
Identities = 14/52 (26%), Positives = 22/52 (41%)
Frame = -1
Query: 186 LYVMGQCEGDVGDSDCGECVKSAVQRAQVECGSSISGQIYLHKCFISFSYYP 237
LY + QC D+ DC +C+ A+ C G++ C + YP
Sbjct: 237 LYGLTQCTRDLSSGDCKKCLDDAINELPNCCDGKEGGRVVGGSCNFRYEIYP 82
Score = 29.3 bits (64), Expect = 0.86
Identities = 15/51 (29%), Positives = 20/51 (38%)
Frame = -1
Query: 84 GIFQCRGDLTNSDCYSCVSKLPVLSDKLCGKTISARVQLSGCYMLYEVAGF 134
G+ QC DL++ DC C+ C RV C YE+ F
Sbjct: 231 GLTQCTRDLSSGDCKKCLDDAINELPNCCDGKEGGRVVGGSCNFRYEIYPF 79
>BP081081
Length = 498
Score = 31.2 bits (69), Expect = 0.23
Identities = 23/76 (30%), Positives = 31/76 (40%), Gaps = 3/76 (3%)
Frame = +3
Query: 55 SALFGSLVSQSTKTRFFKTTTGNGQNSITGIFQCRGDLTNSDCYSCVSKLPVLSDKL--- 111
+ L VS T T F+T T + QCR L+ DC +C +DK
Sbjct: 264 TTLLTCAVSMPTSTTRFRTFT---------MLQCRNYLSRKDCLACFD----AADKKIRN 404
Query: 112 CGKTISARVQLSGCYM 127
C ARV GC++
Sbjct: 405 CSAANGARVIYDGCFL 452
>BF177869
Length = 509
Score = 31.2 bits (69), Expect = 0.23
Identities = 18/30 (60%), Positives = 22/30 (73%), Gaps = 2/30 (6%)
Frame = +2
Query: 244 GH--SSSGGGSSSSSYSSSFSDAMTGQNTG 271
GH SSSG SSSSS SSS SD+ +G ++G
Sbjct: 230 GHASSSSGSSSSSSSGSSSSSDSDSGSSSG 319
>TC7910 homologue to UP|P93166 (P93166) SCOF-1, partial (52%)
Length = 1035
Score = 31.2 bits (69), Expect = 0.23
Identities = 17/55 (30%), Positives = 29/55 (51%), Gaps = 3/55 (5%)
Frame = +3
Query: 214 VECGSSISGQIYLHKCFISFSYYPNGVPKGGHSS---SGGGSSSSSYSSSFSDAM 265
V S+ +G +H+C I +P G GGH GGG +SS+ +++ S+ +
Sbjct: 480 VTTSSASNGGGKVHQCSICQKSFPTGQALGGHKRCHYEGGGGASSTATATASEGV 644
>TC19245 similar to UP|Q99K50 (Q99K50) Nucleolin, partial (3%)
Length = 541
Score = 29.6 bits (65), Expect = 0.66
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +3
Query: 246 SSSGGGSSSSSYSSSFSDAMTGQNTGKTVAIIL 278
SSS SSSS + SS +D N+GK+ A+ L
Sbjct: 120 SSSSSSSSSSYWVSSSADDAAAMNSGKSTAVAL 218
>BP054670
Length = 367
Score = 29.6 bits (65), Expect = 0.66
Identities = 19/43 (44%), Positives = 21/43 (48%)
Frame = +2
Query: 218 SSISGQIYLHKCFISFSYYPNGVPKGGHSSSGGGSSSSSYSSS 260
S S L + S S PN PK G +S SSSSS SSS
Sbjct: 221 SPSSSSSSLERSSTSLSLSPNPGPKRGSTSESSESSSSSLSSS 349
>TC14229 similar to UP|P93166 (P93166) SCOF-1, partial (38%)
Length = 1175
Score = 29.3 bits (64), Expect = 0.86
Identities = 21/62 (33%), Positives = 27/62 (42%), Gaps = 2/62 (3%)
Frame = +1
Query: 226 LHKCFISFSYYPNGVPKGGHSSS--GGGSSSSSYSSSFSDAMTGQNTGKTVAIILGGIAA 283
+H+C I +P G GGH GG SS+S +S+ NT V G AA
Sbjct: 535 MHECSICHKSFPTGQALGGHKRCHYEGGKSSNSTNSN-----VHANTSSAVTTTSDGGAA 699
Query: 284 VA 285
A
Sbjct: 700 SA 705
>BP085186
Length = 388
Score = 28.9 bits (63), Expect = 1.1
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = +3
Query: 246 SSSGGGSSSSSYSSSFSDAMTGQNTGKTVAIILGGIAAVAFLV 288
SSSG SSSSS SSS S + + ++ L G+ ++ F +
Sbjct: 183 SSSGSASSSSSSSSSSSSSSSSSSSSFPSPSALSGLISLPFTI 311
>TC15720 similar to UP|O35328 (O35328) Proline-rich protein 9-1 (Fragment),
partial (11%)
Length = 1115
Score = 28.9 bits (63), Expect = 1.1
Identities = 15/24 (62%), Positives = 17/24 (70%)
Frame = +3
Query: 247 SSGGGSSSSSYSSSFSDAMTGQNT 270
SSGGGSSSSS SSS + +G T
Sbjct: 72 SSGGGSSSSSISSSSAGCFSGYIT 143
>BP049917
Length = 480
Score = 28.5 bits (62), Expect = 1.5
Identities = 22/60 (36%), Positives = 29/60 (47%), Gaps = 5/60 (8%)
Frame = +1
Query: 29 DYTTLVYKGCSKETFTDPN-----GAYSQSLSALFGSLVSQSTKTRFFKTTTGNGQNSIT 83
D T K CS T PN AYS SA+ G + + +T + F+TTT N Q+ T
Sbjct: 280 DSTMAALKPCSTAKLT-PNFMAHASAYSGPKSAVRGLVPAATTFPK*FRTTTXNPQSEPT 456
>TC19515 similar to UP|Q9YGK0 (Q9YGK0) Vitellogenin precursor, partial (3%)
Length = 504
Score = 28.1 bits (61), Expect = 1.9
Identities = 16/26 (61%), Positives = 16/26 (61%)
Frame = -3
Query: 237 PNGVPKGGHSSSGGGSSSSSYSSSFS 262
P PK G SSS SSSSS SSS S
Sbjct: 160 PRPGPKRGSSSSPSSSSSSSSSSSLS 83
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,775,433
Number of Sequences: 28460
Number of extensions: 91222
Number of successful extensions: 1020
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 714
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 879
length of query: 303
length of database: 4,897,600
effective HSP length: 90
effective length of query: 213
effective length of database: 2,336,200
effective search space: 497610600
effective search space used: 497610600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC144807.11