BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.7 - phase: 0
(482 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI418295 79 2e-15
BP030371 68 3e-12
AV779281 66 1e-11
BP041457 64 5e-11
AV777202 58 3e-09
BP033820 41 4e-04
BP054557 39 0.001
BP073434 39 0.002
BP066067 39 0.002
BP030995 36 0.012
AV427609 35 0.021
BI418821 33 0.079
TC12255 33 0.079
AV409763 33 0.10
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 31 0.39
AV420911 31 0.51
AV424294 31 0.51
AV418552 30 0.87
AV408970 29 1.5
TC16450 similar to UP|Q94A73 (Q94A73) AT5g66560/K1F13_23, partia... 28 2.5
>BI418295
Length = 523
Score = 79.0 bits (193), Expect = 2e-15
Identities = 41/165 (24%), Positives = 82/165 (48%), Gaps = 1/165 (0%)
Frame = +2
Query: 28 WKSLVLPVVRGCKLDGYMLGTKKCPEEFITSSDS-SKSNNPAFEEWQANDQRLLGWMLNS 86
W+ V ++ +L +++ + P+ F+T D + + NPA+ W D + W+L +
Sbjct: 14 WRQQVEGAIQSHQLHDFLVDPE-IPQRFLTEEDRVAATENPAYTTWIKQDSMVFTWLLTT 190
Query: 87 MATEMATQLLHCETSKQLWDEAQSLAGAHTRSQIIYLKSEFHSIRKGEMKMEDYLIKMKN 146
++ + +++CE S Q+W E R+Q L+SE +I KG + +YL ++K
Sbjct: 191 LSPFVLPTVINCERSSQVWREILDFFENQCRAQSAKLRSELKNITKGSKSITEYLRRIKT 370
Query: 147 LADKLKLAGNPISNSDLIIQTLNGLDSEYNPIVVKLSDHTTLSWV 191
+ + L G P+S D + +GL +Y+ + + H + S +
Sbjct: 371 IVNTLVSIGEPVSFRDHLEAIFDGLPEDYSALSTIIFTHASSSTI 505
>BP030371
Length = 516
Score = 68.2 bits (165), Expect = 3e-12
Identities = 51/155 (32%), Positives = 81/155 (51%), Gaps = 12/155 (7%)
Frame = -2
Query: 139 DYLIKMKNLADKLKLAGNPISNSDLIIQTLNGLDSEYNPIV--VKLSDH-TTLSWVDLQA 195
+YL+ +K++ D + G+P+S + L G ++Y +V + +SD LS ++++
Sbjct: 503 EYLM*IKSITDPPRSIGDPVSAKEQAEIILEGFPTKYESLVNLIHMSDKFQALSLDEIES 324
Query: 196 QLLTFESRIEQLNN-LTNLNLNATANVANKFDHRDNRF------NSNNNWRG-SNFRGWR 247
L E+RIE LT + +N T + N+ N + ++ + G SNF G R
Sbjct: 323 LLRDQEARIECYRKFLTEI*VNLT*TLENQGSSSANTYAPAPVHSAEASHDGYSNFNGKR 144
Query: 248 -GGRGRGRSSKAPCQVCGKTNHTAINCFHRFDKNY 281
GG+G GR CQVC T HTA C+HRFDK +
Sbjct: 143 CGGKG*GRGYGVQCQVCLPTGHTASVCYHRFDKTF 39
>AV779281
Length = 505
Score = 66.2 bits (160), Expect = 1e-11
Identities = 37/131 (28%), Positives = 66/131 (50%), Gaps = 1/131 (0%)
Frame = +1
Query: 20 LDRNNYPLWKSLVLPVVRGCKLDGYMLGTKKCPEEFITSSDSSKS-NNPAFEEWQANDQR 78
L+ N+ WK V +VR + + L P F++ D + NPA++ W+ D
Sbjct: 103 LEDTNFLSWKQHVEGIVRTHRWQSF-LDHPDVPPRFLSDEDRVNAVENPAYQLWEQQDSA 279
Query: 79 LLGWMLNSMATEMATQLLHCETSKQLWDEAQSLAGAHTRSQIIYLKSEFHSIRKGEMKME 138
L W+L+S++ + +++C S Q+ + AH+ +Q L+SE SI KG
Sbjct: 280 LFTWLLSSLSPTVLPTVVNCSQSWQVREAVLDFFKAHSFAQSTQLRSELRSIAKGSKTCS 459
Query: 139 DYLIKMKNLAD 149
DYL ++K++ +
Sbjct: 460 DYLKRIKSIVN 492
>BP041457
Length = 495
Score = 63.9 bits (154), Expect = 5e-11
Identities = 36/127 (28%), Positives = 64/127 (50%), Gaps = 3/127 (2%)
Frame = +2
Query: 19 KLDRNNYPLWKSLVLPVVRGCKLDGYMLGTKKCPEEFITSSDSSKSN---NPAFEEWQAN 75
KL + LWK V P+++ L Y++ P SSD +N NPA+ W+ +
Sbjct: 122 KLSAKKFLLWKQQVEPIIKAHHLHCYIVEPAIPPRY---SSDEEHANGVVNPAYPIWETH 292
Query: 76 DQRLLGWMLNSMATEMATQLLHCETSKQLWDEAQSLAGAHTRSQIIYLKSEFHSIRKGEM 135
DQ LL W+ + +++ + T ++ C S QLW + S TR++ +++E S + G
Sbjct: 293 DQFLLSWLQSLLSSSVHTCMIGCVNSFQLWVKIHSFFHNQTRAKSTQIRTELRSAKLGGR 472
Query: 136 KMEDYLI 142
+ Y++
Sbjct: 473 LLHGYIL 493
>AV777202
Length = 421
Score = 58.2 bits (139), Expect = 3e-09
Identities = 32/110 (29%), Positives = 59/110 (53%), Gaps = 7/110 (6%)
Frame = +3
Query: 10 NDLPSSVSVKLDRNNYPLWKSLVLPVVRGCKLDGYMLGTKKCPEEFITSSDSSKS-NNPA 68
+ L + +KLD +N+ WK V +VR +L +++ P F++ D + NPA
Sbjct: 84 SSLVHKLVLKLDDSNFLSWKQHVEGIVRTHRLQNFLVHPD-IPPRFLSEEDRVNAVENPA 260
Query: 69 FEEWQANDQRLLGWMLNSMATEMATQLLHCETSKQLWD------EAQSLA 112
++ W D L W+L+S+++ + +++C S Q+W+ +AQSLA
Sbjct: 261 YQVWDQQDSALFTWLLSSLSSSVLPTVVNCTQSWQIWEAVLDFFQAQSLA 410
>BP033820
Length = 537
Score = 41.2 bits (95), Expect = 4e-04
Identities = 19/35 (54%), Positives = 26/35 (74%)
Frame = -2
Query: 361 LNLDDVLYVPNITKNLLSVSKLAADNNIFVEFDKN 395
L L+ +L VP+ITKNL+SVS+ A DNN++ F N
Sbjct: 500 LILNKLLLVPSITKNLVSVSRFAKDNNVYFTFHAN 396
>BP054557
Length = 538
Score = 39.3 bits (90), Expect = 0.001
Identities = 34/84 (40%), Positives = 43/84 (50%), Gaps = 3/84 (3%)
Frame = -2
Query: 314 DSGASNHVTHQTNKFQDLTEHHGKNSLVVGNGDKLEIVATCSSKLKSLN--LDDVLYVPN 371
D+GA++HV H + FQ L + H L + NG K EI S S L VLYVP+
Sbjct: 531 DTGATHHVCHNLDVFQALRQVHPV-LLSMPNGQK-EIAHMADSVYFSEKFFLAXVLYVPS 358
Query: 372 ITKNLLSVSKLAADNNI-FVEFDK 394
NL+SVS L N V FD+
Sbjct: 357 FNFNLISVSALVKSLNCRLVIFDE 286
>BP073434
Length = 490
Score = 38.9 bits (89), Expect = 0.002
Identities = 18/43 (41%), Positives = 24/43 (54%)
Frame = -2
Query: 311 WYFDSGASNHVTHQTNKFQDLTEHHGKNSLVVGNGDKLEIVAT 353
WY DSGAS+HVT N Q G + + VGNG L + ++
Sbjct: 456 WYPDSGASHHVTPHGNNIQYPDPFEGPDHIFVGNGQGLSVASS 328
>BP066067
Length = 505
Score = 38.5 bits (88), Expect = 0.002
Identities = 25/76 (32%), Positives = 40/76 (51%), Gaps = 7/76 (9%)
Frame = -2
Query: 309 YDWYFDSGASNHVTHQTNKFQDLTEHHGKNSLVVGNGDKLEIVA----TCSSKLKS---L 361
+ W DSGAS+HVT+ + G+ + V +G L I++ + SS L S
Sbjct: 303 HSW*ADSGASHHVTNDPQHLMESITMPGQEQVYVCSGQGLPILSIGFTSFSSPLISNTKF 124
Query: 362 NLDDVLYVPNITKNLL 377
+L D ++P ITKN++
Sbjct: 123 SLKDFFHLPTITKNIV 76
>BP030995
Length = 458
Score = 36.2 bits (82), Expect = 0.012
Identities = 18/38 (47%), Positives = 24/38 (62%)
Frame = -3
Query: 355 SSKLKSLNLDDVLYVPNITKNLLSVSKLAADNNIFVEF 392
SS L L+++L+VP + KNL SVS NN+F EF
Sbjct: 444 SSTSFPLVLNNLLHVPGVCKNLSSVSXFCKQNNVFFEF 331
>AV427609
Length = 332
Score = 35.4 bits (80), Expect = 0.021
Identities = 15/67 (22%), Positives = 36/67 (53%), Gaps = 1/67 (1%)
Frame = +1
Query: 66 NPAFEEWQANDQRLLGWMLNSMATEMATQLLH-CETSKQLWDEAQSLAGAHTRSQIIYLK 124
+PA+E+W D +L W+ ++++ ++ T ++ T+ W L + ++ + L+
Sbjct: 76 DPAYEQWATLDATVLQWIYSTISVDLMTTIMEPGSTAFTAWTHLADLFQDNQNARAVTLE 255
Query: 125 SEFHSIR 131
EF ++R
Sbjct: 256 QEFSTVR 276
>BI418821
Length = 614
Score = 33.5 bits (75), Expect = 0.079
Identities = 18/42 (42%), Positives = 20/42 (46%), Gaps = 4/42 (9%)
Frame = +2
Query: 243 FRGWRGGR----GRGRSSKAPCQVCGKTNHTAINCFHRFDKN 280
F GWRGG G G C CG T H A +C HR + N
Sbjct: 329 FGGWRGGERRNGGGGGGGGGGCYNCGDTGHLARDC-HRSNNN 451
>TC12255
Length = 558
Score = 33.5 bits (75), Expect = 0.079
Identities = 17/41 (41%), Positives = 24/41 (58%)
Frame = +3
Query: 310 DWYFDSGASNHVTHQTNKFQDLTEHHGKNSLVVGNGDKLEI 350
+W DSGA++HVT+ F D ++ L+VGNG L I
Sbjct: 48 NWCPDSGATHHVTNNPGIFVDNVPLATQDQLLVGNGQGLPI 170
>AV409763
Length = 426
Score = 33.1 bits (74), Expect = 0.10
Identities = 22/80 (27%), Positives = 41/80 (50%), Gaps = 1/80 (1%)
Frame = +2
Query: 14 SSVSVKLDRNNYPLW-KSLVLPVVRGCKLDGYMLGTKKCPEEFITSSDSSKSNNPAFEEW 72
S VS L +NY W +++ + ++ KL G++ + P+E +P F W
Sbjct: 215 SLVSQVLTGDNYRSWSRAMRMALIAKNKL-GFVDASILVPDEL----------DPLFHPW 361
Query: 73 QANDQRLLGWMLNSMATEMA 92
Q N+ + W+LNS++ ++A
Sbjct: 362 QRNNNIVASWILNSVSKDLA 421
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 31.2 bits (69), Expect = 0.39
Identities = 18/50 (36%), Positives = 21/50 (42%), Gaps = 6/50 (12%)
Frame = +1
Query: 233 NSNNNWR---GSNFRGWRGGRGRGRS---SKAPCQVCGKTNHTAINCFHR 276
+ N WR N +G GGRG GR C CG+ H A C R
Sbjct: 67 DGKNGWRVELSHNSKGGGGGRGGGRGRGGEDLKCYECGEPGHFARECRSR 216
>AV420911
Length = 418
Score = 30.8 bits (68), Expect = 0.51
Identities = 17/47 (36%), Positives = 20/47 (42%), Gaps = 6/47 (12%)
Frame = +2
Query: 233 NSNNNWR---GSNFRGWRGGRGRGRS---SKAPCQVCGKTNHTAINC 273
+ N WR N +G GGRG GR C CG+ H A C
Sbjct: 275 DGKNGWRVELSHNSKGGGGGRGGGRGRGGEDLKCYECGEPGHFAREC 415
>AV424294
Length = 395
Score = 30.8 bits (68), Expect = 0.51
Identities = 28/102 (27%), Positives = 43/102 (41%), Gaps = 1/102 (0%)
Frame = -2
Query: 281 YSRSNYSADSDKQGSHNAFIASQNSVEDYDWYFDSGAS-NHVTHQTNKFQDLTEHHGKNS 339
YS+S + A GSH F + W+ SGAS N T+ ++E
Sbjct: 328 YSQSMFIAI*TSDGSHTKFSLFVSITVHLCWFLYSGASLNSKKESTSASVSISEILLARD 149
Query: 340 LVVGNGDKLEIVATCSSKLKSLNLDDVLYVPNITKNLLSVSK 381
+ +GD+ + T + KS++L+ NIT L SK
Sbjct: 148 RIALDGDEKRRIKTVKTITKSIDLE-----RNITTTFLFQSK 38
>AV418552
Length = 386
Score = 30.0 bits (66), Expect = 0.87
Identities = 19/65 (29%), Positives = 27/65 (41%)
Frame = +3
Query: 208 NNLTNLNLNATANVANKFDHRDNRFNSNNNWRGSNFRGWRGGRGRGRSSKAPCQVCGKTN 267
NN + NL+ + + + +N NSNNNWR N SS C+ N
Sbjct: 177 NNSRSCNLHCSNSSN*HSSNNNNLHNSNNNWRSCNLH----NINTHNSSNNSCRSSSFNN 344
Query: 268 HTAIN 272
+IN
Sbjct: 345 FLSIN 359
>AV408970
Length = 195
Score = 29.3 bits (64), Expect = 1.5
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -3
Query: 238 WRGSNFRGWRGGRGRG 253
WRG GWRGGRG G
Sbjct: 169 WRGWRGSGWRGGRGGG 122
>TC16450 similar to UP|Q94A73 (Q94A73) AT5g66560/K1F13_23, partial (10%)
Length = 528
Score = 28.5 bits (62), Expect = 2.5
Identities = 17/49 (34%), Positives = 20/49 (40%)
Frame = +2
Query: 245 GWRGGRGRGRSSKAPCQVCGKTNHTAINCFHRFDKNYSRSNYSADSDKQ 293
GWR GR S K QVC T + DK R ++ A KQ
Sbjct: 230 GWRASLGRKFSCKFKTQVCDSQQSTVV------DKRKGRHHHRAPPKKQ 358
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.130 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,981,685
Number of Sequences: 28460
Number of extensions: 103391
Number of successful extensions: 716
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 703
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 710
length of query: 482
length of database: 4,897,600
effective HSP length: 94
effective length of query: 388
effective length of database: 2,222,360
effective search space: 862275680
effective search space used: 862275680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC144765.7