Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144765.21 - phase: 0 
         (471 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC11811 similar to PIR|D96538|D96538 cytosolic tRNA-Ala syntheta...   342  8e-95
TC10586 similar to GB|CAA80380.1|1673365|ATTRNASYA mitochondrial...   159  9e-40
AV409818                                                               48  3e-06
TC7848 homologue to UP|Q9AVR8 (Q9AVR8) Sucrose synthase isoform ...    28  3.2
TC9931 weakly similar to GB|AAO39953.1|28372942|BT003725 At5g152...    28  3.2
TC9135 homologue to UP|Q9SBJ1 (Q9SBJ1) Pyruvate dehydrogenase ki...    27  7.2
TC12030                                                                27  7.2

>TC11811 similar to PIR|D96538|D96538 cytosolic tRNA-Ala synthetase
           [imported] - Arabidopsis                thaliana
           {Arabidopsis thaliana;}, partial (18%)
          Length = 680

 Score =  342 bits (877), Expect = 8e-95
 Identities = 156/184 (84%), Positives = 166/184 (89%), Gaps = 1/184 (0%)
 Frame = +2

Query: 1   MPETESIDIEWPAKRVRDTFAKFFEE-KNHVHWKSSAVVPLNDPTLLFANAGMNQFKPIF 59
           MP  +++D+EWPAKRVRDTF  FF+E KNHV+W+SS VVP NDPTLLFANAGMNQFKPIF
Sbjct: 128 MPGPDALDLEWPAKRVRDTFVNFFKESKNHVYWQSSPVVPFNDPTLLFANAGMNQFKPIF 307

Query: 60  LGTVDPNTALSKLTRACNIQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKEE 119
           LGT DPNT LSKL+RACN QKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFK E
Sbjct: 308 LGTADPNTDLSKLSRACNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKTE 487

Query: 120 AISWAWELLTEVYNLPSDRIYVTYFGGDEKTGLGPDNEARDIWLKFLPPGRVLPFGSKDN 179
           AI WAWELLT+VY LPSDRIY TYFGGDEK GL PD EARDIWL+FLPPGRVLPFG KDN
Sbjct: 488 AIGWAWELLTKVYKLPSDRIYATYFGGDEKAGLAPDTEARDIWLQFLPPGRVLPFGCKDN 667

Query: 180 FWEM 183
           FWEM
Sbjct: 668 FWEM 679


>TC10586 similar to GB|CAA80380.1|1673365|ATTRNASYA mitochondrial tRNA-Ala
           synthetase {Arabidopsis thaliana;} , partial (9%)
          Length = 644

 Score =  159 bits (402), Expect = 9e-40
 Identities = 76/91 (83%), Positives = 85/91 (92%)
 Frame = +1

Query: 202 NRNASSFVNDEDPNCIELWNLVFIQFNREADGSLKSLPAKHVDTGMGFERLTSILQHKNS 261
           NR+A+S VN++DP CIE+WNLVFIQFNREADGSLKSLPAKHVDTGMGFERLTSILQ++ S
Sbjct: 1   NRDAASLVNNDDPTCIEIWNLVFIQFNREADGSLKSLPAKHVDTGMGFERLTSILQNQMS 180

Query: 262 NYDTDVFVPIFDAIQQATGARPYSGKVGPHD 292
           +YDTDVF+PIFDAIQ ATGARPYSGKVG  D
Sbjct: 181 HYDTDVFLPIFDAIQLATGARPYSGKVGA*D 273


>AV409818 
          Length = 426

 Score = 48.1 bits (113), Expect = 3e-06
 Identities = 22/48 (45%), Positives = 33/48 (67%)
 Frame = +2

Query: 16  VRDTFAKFFEEKNHVHWKSSAVVPLNDPTLLFANAGMNQFKPIFLGTV 63
           +RD F +F+  + H    S++++P +DPT+L   AGM QFKPIFLG +
Sbjct: 266 IRDRFLRFYASRGHKVLPSASLIP-DDPTVLLTIAGMLQFKPIFLGKI 406


>TC7848 homologue to UP|Q9AVR8 (Q9AVR8) Sucrose synthase isoform 3  ,
           complete
          Length = 2779

 Score = 28.1 bits (61), Expect = 3.2
 Identities = 14/27 (51%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
 Frame = -1

Query: 423 GFPLDLTQLMAEEKKF-SVDIEGFHRA 448
           GFP    Q + EE KF S D +GFH+A
Sbjct: 199 GFPCPWLQSLTEESKFHSFDQQGFHQA 119


>TC9931 weakly similar to GB|AAO39953.1|28372942|BT003725 At5g15290
           {Arabidopsis thaliana;}, partial (60%)
          Length = 609

 Score = 28.1 bits (61), Expect = 3.2
 Identities = 12/31 (38%), Positives = 19/31 (60%)
 Frame = -1

Query: 395 IRKFNKAVEHVQGNILSGEATFELSHTFGFP 425
           ++K N ++EH   NI S +A +  SHT  +P
Sbjct: 510 VKKTNTSIEHQTENI*STQAKWNTSHTHFYP 418


>TC9135 homologue to UP|Q9SBJ1 (Q9SBJ1) Pyruvate dehydrogenase kinase  ,
           partial (31%)
          Length = 538

 Score = 26.9 bits (58), Expect = 7.2
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = +1

Query: 132 YNLPSDRIYVTYFGGD 147
           Y +P  R+Y  YFGGD
Sbjct: 220 YGIPISRLYARYFGGD 267


>TC12030 
          Length = 401

 Score = 26.9 bits (58), Expect = 7.2
 Identities = 16/57 (28%), Positives = 25/57 (43%)
 Frame = -3

Query: 77  NIQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKEEAISWAWELLTEVYN 133
           NIQ+  + G  +   DD+  D  +     +  NW  G  F E+ +S    L  E +N
Sbjct: 357 NIQQ*SKRGNHNTMADDITSDIMNK-IVPLTSNWITGSSFNEQPVSTEHILKIEQFN 190


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.320    0.139    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,178,256
Number of Sequences: 28460
Number of extensions: 113907
Number of successful extensions: 431
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 430
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 430
length of query: 471
length of database: 4,897,600
effective HSP length: 94
effective length of query: 377
effective length of database: 2,222,360
effective search space: 837829720
effective search space used: 837829720
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)


Medicago: description of AC144765.21