BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.20 + phase: 0
(296 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV413186 94 2e-20
AU089102 90 5e-19
AI967878 57 5e-09
AV766731 54 3e-08
BP081623 52 2e-07
BP038410 52 2e-07
TC19836 49 1e-06
AV425299 44 2e-05
AV769479 44 2e-05
AV767495 37 0.004
AV772727 35 0.012
BU494388 34 0.026
BP047039 34 0.034
AV422239 32 0.13
AV429083 29 0.83
TC8881 homologue to UP|ILV5_PEA (O82043) Ketol-acid reductoisome... 29 1.1
CB826792 28 1.4
BP052640 28 1.9
AV768678 28 2.4
BP029246 27 3.2
>AV413186
Length = 398
Score = 94.4 bits (233), Expect = 2e-20
Identities = 47/118 (39%), Positives = 67/118 (55%), Gaps = 1/118 (0%)
Frame = +1
Query: 84 NMYCKCGNLSLAWEIFNGMKMKT-VSCWNAMVIGLAVHGYCEEVFQLFTEMEESLGGSIR 142
+MY KCG+L AW +FN + K V WN M+ LA HG+ E + +M S +
Sbjct: 4 DMYSKCGSLETAWRVFNLVGNKQDVVLWNTMISALAHHGFGTEAMVMLNDMIRS---GVE 174
Query: 143 PDRVTFIGVLVACSHKGLVDKARWYFDHMVKRYKIVPDSKHYGCMVDLLSRWGLLEEA 200
P+R TF+ +L ACS GLV + +F M + +VPD +HY C+ DLL R G +E+
Sbjct: 175 PNRATFVVLLNACSLSGLVQEGLQFFKFMTSEFGVVPDQEHYACLTDLLVRAGYFDES 348
Score = 28.5 bits (62), Expect = 1.4
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +1
Query: 189 DLLSRWGLLEEAYQIIMTAPFQNSVVLWRTLLGA 222
D+ S+ G LE A+++ + VVLW T++ A
Sbjct: 4 DMYSKCGSLETAWRVFNLVGNKQDVVLWNTMISA 105
>AU089102
Length = 480
Score = 89.7 bits (221), Expect = 5e-19
Identities = 44/118 (37%), Positives = 65/118 (54%)
Frame = +2
Query: 146 VTFIGVLVACSHKGLVDKARWYFDHMVKRYKIVPDSKHYGCMVDLLSRWGLLEEAYQIIM 205
VT++ +L ACSH GLVD+ YFD ++K I HY C+VDL R G L+E + II
Sbjct: 44 VTYVELLTACSHAGLVDEGIQYFDKLLKNRSIQVKEXHYACLVDLCGRAGRLKEXFYIIE 223
Query: 206 TAPFQNSVVLWRTLLGACRTQSNTELAEISFKQLAKLKQLIDGDYVLLSNIYAEAGRW 263
+ S+ +W LL C N ++ ++ ++ K++ G Y LLSN+YA W
Sbjct: 224 GLGVKLSLSVWGPLLAGCNVHGNADIGKLVTTKILKVEHENAGTYSLLSNMYASXRXW 397
>AI967878
Length = 393
Score = 56.6 bits (135), Expect = 5e-09
Identities = 37/116 (31%), Positives = 52/116 (43%), Gaps = 17/116 (14%)
Frame = +1
Query: 110 WNAMVIGLAVHGYCEEVFQLFTEME--ESLGGSIR---------------PDRVTFIGVL 152
WNAM++G +HG E+ LF EM + + G + PD VTFIG+L
Sbjct: 46 WNAMILGHCIHGNPEDGLSLFDEMVGMDKVKGEVEIDESPCADRGVVRLPPDEVTFIGIL 225
Query: 153 VACSHKGLVDKARWYFDHMVKRYKIVPDSKHYGCMVDLLSRWGLLEEAYQIIMTAP 208
AC+ L+ + R YF M + + P YG L GLL + + T P
Sbjct: 226 CACARAELLAEGRSYFKQMTDVFGLKPILLIYGAWPICLQMHGLLMKPKSVCGTWP 393
Score = 35.0 bits (79), Expect = 0.015
Identities = 28/84 (33%), Positives = 34/84 (40%), Gaps = 20/84 (23%)
Frame = +1
Query: 4 RNAVSWNSMIAGCVSVRDYAGALELFSEM-----------------QNAGV---KPTEVT 43
RN VSWN+MI G + L LF EM + GV P EVT
Sbjct: 31 RNLVSWNAMILGHCIHGNPEDGLSLFDEMVGMDKVKGEVEIDESPCADRGVVRLPPDEVT 210
Query: 44 LISILGACAETGALEIGHKIYESL 67
I IL ACA L G ++ +
Sbjct: 211 FIGILCACARAELLAEGRSYFKQM 282
>AV766731
Length = 576
Score = 53.9 bits (128), Expect = 3e-08
Identities = 49/177 (27%), Positives = 81/177 (45%), Gaps = 7/177 (3%)
Frame = -3
Query: 5 NAVSWNSMIAGCVSVRDYAGALELFSEMQNAGVKPTEVTLISILGACAETGALEIGHKIY 64
N V NS+I G R + A+EL M G +T S+ + L+ ++
Sbjct: 550 NTVKKNSLINGLCKARRISCAVELVDVMHETGPPADLITTNSLFDGLCKHHLLDKAIALF 371
Query: 65 ESLKVCEHKIESYLGN--ALVNMYCKCGNLSLAWEIFNGMKMKTVSCWNAMVIGLAVHGY 122
+KV + KI+ + +++ CK G L +A EIF + + + N M + ++GY
Sbjct: 370 --MKVKDPKIQPNIPPYPVIIDGLCKVGRLKIAQEIFQVLLSEGYNL-NVMTYTVMINGY 200
Query: 123 C-----EEVFQLFTEMEESLGGSIRPDRVTFIGVLVACSHKGLVDKARWYFDHMVKR 174
C +E L ++ME++ G I P+ V F ++ A K DKA F M+ R
Sbjct: 199 CKEGLLDEAQALLSKMEDN--GCI-PNAVNFQTIICALFEKNENDKAERLFREMIAR 38
>BP081623
Length = 461
Score = 51.6 bits (122), Expect = 2e-07
Identities = 26/75 (34%), Positives = 40/75 (52%)
Frame = -2
Query: 215 LWRTLLGACRTQSNTELAEISFKQLAKLKQLIDGDYVLLSNIYAEAGRWDEVERLRSEMD 274
+W LLGACR + L + + L +LK G Y+LL+N+YAE RWD+ ++R M
Sbjct: 460 VWGALLGACRIYGDVGLGRWAAEHLFELKPQHCGYYILLANMYAEIERWDDANKVRQLMK 281
Query: 275 YLHVPRQAGYSQINM 289
+ G S + +
Sbjct: 280 SRGAKKNPGCSWVKV 236
>BP038410
Length = 468
Score = 51.6 bits (122), Expect = 2e-07
Identities = 40/154 (25%), Positives = 71/154 (45%), Gaps = 1/154 (0%)
Frame = -2
Query: 2 PERN-AVSWNSMIAGCVSVRDYAGALELFSEMQNAGVKPTEVTLISILGACAETGALEIG 60
PE N VSWNS+ +LF++M +G+ + T++ +L A +TG +G
Sbjct: 416 PEGNDLVSWNSVF-------------DLFTKMCWSGLIASASTVLILLPAAGDTGNFVVG 276
Query: 61 HKIYESLKVCEHKIESYLGNALVNMYCKCGNLSLAWEIFNGMKMKTVSCWNAMVIGLAVH 120
++ + AL++MY K G + +IF+ + K V WN ++ A +
Sbjct: 275 KSLHGYYIQIGFSSHLNVLTALIDMYAKTGLVHWGRKIFDSLVDKDVVLWNCLIGNYARN 96
Query: 121 GYCEEVFQLFTEMEESLGGSIRPDRVTFIGVLVA 154
G E L +M ++ + TF+G+L+A
Sbjct: 95 GLVGEALYLLQKMRIQ---GMKTNSSTFVGLLLA 3
>TC19836
Length = 464
Score = 48.5 bits (114), Expect = 1e-06
Identities = 25/82 (30%), Positives = 43/82 (51%)
Frame = +2
Query: 206 TAPFQNSVVLWRTLLGACRTQSNTELAEISFKQLAKLKQLIDGDYVLLSNIYAEAGRWDE 265
T P + S +W +L+ AC+ ++AE+ QL + + +Y LL+ IYAE G W
Sbjct: 5 TMPMKPSTRIWSSLVSACKLHGRLDIAEMLGPQLIRSEPDNAANYTLLNMIYAEHGHWLH 184
Query: 266 VERLRSEMDYLHVPRQAGYSQI 287
E++ M + + G+S+I
Sbjct: 185 KEQVMEAMKLQRLKKCYGFSRI 250
>AV425299
Length = 323
Score = 44.3 bits (103), Expect = 2e-05
Identities = 28/90 (31%), Positives = 47/90 (52%), Gaps = 4/90 (4%)
Frame = +3
Query: 74 IESYLGNALVNMYCKCGNLSLAWEIFNGMKMK----TVSCWNAMVIGLAVHGYCEEVFQL 129
+++ +ALV++YCK GNL A E F + K + +N+M++G G + L
Sbjct: 21 LDASTASALVHLYCKVGNLERAKEAFEVLTRKGFQPDIKVYNSMIMGYVNAGDPKAGESL 200
Query: 130 FTEMEESLGGSIRPDRVTFIGVLVACSHKG 159
+MEE G I+P + + +L A S +G
Sbjct: 201 MKKMEER--GGIKPTKEIYTALLRAYSERG 284
Score = 30.0 bits (66), Expect = 0.49
Identities = 17/48 (35%), Positives = 26/48 (53%), Gaps = 1/48 (2%)
Frame = +3
Query: 9 WNSMIAGCVSVRDYAGALELFSEMQN-AGVKPTEVTLISILGACAETG 55
+NSMI G V+ D L +M+ G+KPT+ ++L A +E G
Sbjct: 141 YNSMIMGYVNAGDPKAGESLMKKMEERGGIKPTKEIYTALLRAYSERG 284
>AV769479
Length = 287
Score = 44.3 bits (103), Expect = 2e-05
Identities = 22/90 (24%), Positives = 43/90 (47%)
Frame = +1
Query: 44 LISILGACAETGALEIGHKIYESLKVCEHKIESYLGNALVNMYCKCGNLSLAWEIFNGMK 103
L+ + C++ L +G +I+ + + + NA VN Y +CG +S A+ F+ M
Sbjct: 4 LVCHMNLCSKRVDLALGKQIHAHILKSKWR-NLIADNAFVNFYAECGKISSAFRTFDRMA 180
Query: 104 MKTVSCWNAMVIGLAVHGYCEEVFQLFTEM 133
+ V CW ++ + G E + ++M
Sbjct: 181 KRDVVCWTTIITACSQQGLGHEALLILSQM 270
>AV767495
Length = 586
Score = 37.0 bits (84), Expect = 0.004
Identities = 40/158 (25%), Positives = 76/158 (47%), Gaps = 3/158 (1%)
Frame = +2
Query: 3 ERNAVSWNSMIAGCVSVRDYAGALELFSEMQNAGVKPTEVTLISILGACAETGALEIGHK 62
E + V ++++I ++ L M G++P VT SI+ A + ALE G
Sbjct: 20 EADVVFYSALIDALCKNGLVESSMVLLDAMIEKGIRPNVVTYNSIIDAFGQLSALECG-- 193
Query: 63 IYESLKVCEHKIESYLGNALVNMYCKCGNLSLAWEIFNGMKM-KTVSCWNAMVIGLAVHG 121
+ S++ EH++ + ++ + + NL++ E MKM + ++ + I + G
Sbjct: 194 VDTSVQANEHRV---VPSSSMLIDGALQNLAIGKEDDRIMKMFEQLAAEKSGQIKKDMRG 364
Query: 122 YCEE--VFQLFTEMEESLGGSIRPDRVTFIGVLVACSH 157
++ + LF +M E I+P+ VTF +L ACS+
Sbjct: 365 SQDKFCILWLFRKMHEM---EIKPNVVTFSAILNACSN 469
Score = 33.5 bits (75), Expect = 0.044
Identities = 15/49 (30%), Positives = 28/49 (56%)
Frame = +2
Query: 26 LELFSEMQNAGVKPTEVTLISILGACAETGALEIGHKIYESLKVCEHKI 74
L LF +M +KP VT +IL AC+ + E K+ + L++ ++++
Sbjct: 386 LWLFRKMHEMEIKPNVVTFSAILNACSNCKSFEDASKLLDELRLFDNQV 532
>AV772727
Length = 528
Score = 35.4 bits (80), Expect = 0.012
Identities = 23/95 (24%), Positives = 44/95 (46%), Gaps = 4/95 (4%)
Frame = -2
Query: 42 VTLISILGACAETGALEIGHKIYESLKVCEHKIESYLGNALVNMYCKCGNLSLAWEIFNG 101
+T S+L A + ++ + E +K + + Y N +++ CK G + A E+F
Sbjct: 524 ITYHSLLDALCKNHHVDKAIALLERVKDKGIQPDMYTYNIIIDGLCKSGRVKDAQELFQD 345
Query: 102 MKMK----TVSCWNAMVIGLAVHGYCEEVFQLFTE 132
+ +K V +N M+ GL + G +E L +
Sbjct: 344 LLIKGYRLNVVTYNIMINGLCIEGLSDEALALLIQ 240
>BU494388
Length = 350
Score = 34.3 bits (77), Expect = 0.026
Identities = 13/63 (20%), Positives = 34/63 (53%)
Frame = +2
Query: 9 WNSMIAGCVSVRDYAGALELFSEMQNAGVKPTEVTLISILGACAETGALEIGHKIYESLK 68
++ +I C +++ + +F+ M+ GVKP+ + S++ AC + + + ++E ++
Sbjct: 137 FSELILLCGKLKNLPLGMRVFTSMETIGVKPSSLVFNSLISACLSSNDIVTAYSLFEIME 316
Query: 69 VCE 71
E
Sbjct: 317 SSE 325
>BP047039
Length = 527
Score = 33.9 bits (76), Expect = 0.034
Identities = 22/65 (33%), Positives = 33/65 (49%)
Frame = -3
Query: 110 WNAMVIGLAVHGYCEEVFQLFTEMEESLGGSIRPDRVTFIGVLVACSHKGLVDKARWYFD 169
+N M+ GL G +E L ++ME++ G I D +TF ++ A KG DKA
Sbjct: 486 YNTMINGLCKEGLFDEALTLMSKMEDN--GCIH-DPITFETIISALFEKGENDKAEKLLR 316
Query: 170 HMVKR 174
M+ R
Sbjct: 315 EMIAR 301
>AV422239
Length = 387
Score = 32.0 bits (71), Expect = 0.13
Identities = 24/98 (24%), Positives = 48/98 (48%), Gaps = 2/98 (2%)
Frame = +2
Query: 8 SWNSMIAGCVSVRDYAGALELFSEMQNAGVKPTEVTLISILGACAETGALEIGHKIYESL 67
+++ MI +R Y ++ ++M+N G+ E T ++ A A ++ +Y
Sbjct: 26 AYHLMIESLARIRQYQIMWDIVTKMRNKGMLNVE-TFCIVMRKYAR--AHKVDEAVYTFN 196
Query: 68 KVCEHKIESYLG--NALVNMYCKCGNLSLAWEIFNGMK 103
+ ++++ L N L++ CK N+ A EIF+ MK
Sbjct: 197 VMDKYEVPQNLAAFNGLLSALCKSRNVRKAQEIFDSMK 310
>AV429083
Length = 316
Score = 29.3 bits (64), Expect = 0.83
Identities = 22/61 (36%), Positives = 32/61 (52%)
Frame = +3
Query: 129 LFTEMEESLGGSIRPDRVTFIGVLVACSHKGLVDKARWYFDHMVKRYKIVPDSKHYGCMV 188
LF EME L +I+PD TF ++ A KG V A+ M+K+ + PD Y ++
Sbjct: 3 LFKEME--LKNNIKPDVSTFNILVDALCKKGKVKHAKNVLAVMIKQ-GVAPDLVTYSTLL 173
Query: 189 D 189
D
Sbjct: 174 D 176
>TC8881 homologue to UP|ILV5_PEA (O82043) Ketol-acid reductoisomerase,
chloroplast precursor (Acetohydroxy-acid
reductoisomerase) (Alpha-keto-beta-hydroxylacil
reductoisomerase) , partial (52%)
Length = 1310
Score = 28.9 bits (63), Expect = 1.1
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 2/100 (2%)
Frame = +2
Query: 118 AVHGYCEEVFQLFTE--MEESLGGSIRPDRVTFIGVLVACSHKGLVDKARWYFDHMVKRY 175
AVHG E +F+ +TE M E L + +T I + S KG++ + + + +
Sbjct: 98 AVHGIVESLFRRYTENGMAEDLAYKNTVESITGI-ISKTISTKGMLAVYNSFSEEGKREF 274
Query: 176 KIVPDSKHYGCMVDLLSRWGLLEEAYQIIMTAPFQNSVVL 215
+ + Y CM +L E Y+ + + SVVL
Sbjct: 275 EKAYSASFYPCM-------DILYECYEDVASGSEIRSVVL 373
>CB826792
Length = 485
Score = 28.5 bits (62), Expect = 1.4
Identities = 20/60 (33%), Positives = 30/60 (49%), Gaps = 4/60 (6%)
Frame = +3
Query: 80 NALVNMYCKCGNLSLAWEIFNGMKMK----TVSCWNAMVIGLAVHGYCEEVFQLFTEMEE 135
N+L+ CK + A E+FN M + T+ ++A L V EEVF+L +M E
Sbjct: 90 NSLIKPLCKNRKIDEAKEVFNDMMKRNITPTIRTFHAFFRILRVE---EEVFELLDKMRE 260
>BP052640
Length = 473
Score = 28.1 bits (61), Expect = 1.9
Identities = 15/39 (38%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +1
Query: 159 GLVDKARWYFDHMVKRYKIVPDSKH-YGCMVDLLSRWGL 196
GLV+K+ W F H+ KR + DS++ + +V L W L
Sbjct: 85 GLVEKSPWGFYHITKREQFN*DSEYQFQLLVPWLLPWAL 201
>AV768678
Length = 429
Score = 27.7 bits (60), Expect = 2.4
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -2
Query: 111 NAMVIGLAVHGYCEEVFQLFTEMEES 136
N V+GLAV CEE F+ F ++ES
Sbjct: 290 NRFVVGLAVEA*CEESFR*FCWLKES 213
>BP029246
Length = 455
Score = 27.3 bits (59), Expect = 3.2
Identities = 15/41 (36%), Positives = 23/41 (55%), Gaps = 1/41 (2%)
Frame = -1
Query: 119 VHGYCEEVFQLFTEMEESL-GGSIRPDRVTFIGVLVACSHK 158
V+G + LF +E+ L GGSI P +T ++ AC H+
Sbjct: 422 VNGLPDTATMLFATLEDLLMGGSILPWTITSKNLMFACLHQ 300
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.136 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,296,515
Number of Sequences: 28460
Number of extensions: 71807
Number of successful extensions: 298
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 290
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 294
length of query: 296
length of database: 4,897,600
effective HSP length: 90
effective length of query: 206
effective length of database: 2,336,200
effective search space: 481257200
effective search space used: 481257200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC144765.20