BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.19 - phase: 0
(146 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 28 0.48
AV766642 28 0.48
AU252157 28 0.82
TC9742 similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin PP2-... 28 0.82
TC9838 similar to UP|Q96347 (Q96347) SLL2, partial (28%) 27 1.4
BP054432 26 3.1
BP079801 26 3.1
TC14103 similar to UP|Q84XC6 (Q84XC6) Plasma intrinsic protein 2... 26 3.1
TC12135 similar to GB|AAP37803.1|30725562|BT008444 At4g35750 {Ar... 26 3.1
TC9848 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lect... 26 3.1
BP052246 25 4.1
BP033771 25 4.1
TC16522 similar to UP|Q84PB7 (Q84PB7) Inositol phosphatase-like ... 25 4.1
TC11653 similar to UP|O33616 (O33616) Histidine kinase, partial ... 25 5.3
TC8642 similar to UP|Q9ASR5 (Q9ASR5) AT4g35920/F4B14_190, partia... 25 5.3
TC14611 similar to UP|Q8LPB2 (Q8LPB2) Glycine-rich RNA binding p... 25 5.3
AV427932 25 7.0
AV780676 25 7.0
TC12733 similar to UP|Q9SAS9 (Q9SAS9) Heat shock protein 90-2 (F... 25 7.0
TC18671 UP|Q40198 (Q40198) RAB11H, complete 25 7.0
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 28.5 bits (62), Expect = 0.48
Identities = 18/77 (23%), Positives = 35/77 (45%)
Frame = +1
Query: 3 KERGVAPFSKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAI 62
K+R + P S ELP + + + Y E V+ EEE +E+ ++
Sbjct: 109 KKRKLVPKSSQSAELPFKLQNKLEAEVQEYEEVEEEVEEEVEEEEEEEEEEEEGEEEEEE 288
Query: 63 EGFKQLLDEASEDIDDK 79
E ++ ++ +E+ DD+
Sbjct: 289 EEEEEEVEAEAEEEDDE 339
>AV766642
Length = 342
Score = 28.5 bits (62), Expect = 0.48
Identities = 8/19 (42%), Positives = 14/19 (73%)
Frame = +2
Query: 125 FIFFVCDSHNHHPYHWITY 143
F+FFV + +H P+HW+ +
Sbjct: 281 FVFFVWNQSSHLPFHWLQF 337
>AU252157
Length = 350
Score = 27.7 bits (60), Expect = 0.82
Identities = 11/28 (39%), Positives = 16/28 (56%)
Frame = -3
Query: 119 FLNSLNFIFFVCDSHNHHPYHWITYLDF 146
F N L+ + +C+ H+P I YLDF
Sbjct: 189 FQNPLDILLSLCEGEIHNPQPCICYLDF 106
>TC9742 similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin PP2-like
protein, partial (20%)
Length = 794
Score = 27.7 bits (60), Expect = 0.82
Identities = 13/31 (41%), Positives = 18/31 (57%)
Frame = +3
Query: 106 LLNERCHFEFNWTFLNSLNFIFFVCDSHNHH 136
L + C F NWT L + N IFF+ +S+ H
Sbjct: 120 LFLDTCRFS-NWTSLQAKNLIFFLQESYR*H 209
>TC9838 similar to UP|Q96347 (Q96347) SLL2, partial (28%)
Length = 562
Score = 26.9 bits (58), Expect = 1.4
Identities = 8/33 (24%), Positives = 17/33 (51%)
Frame = +3
Query: 110 RCHFEFNWTFLNSLNFIFFVCDSHNHHPYHWIT 142
RC FN +++ + ++C HP+ W++
Sbjct: 387 RCILTFNVMLVSNRKYFSYLCGGKFPHPFFWVS 485
>BP054432
Length = 369
Score = 25.8 bits (55), Expect = 3.1
Identities = 8/24 (33%), Positives = 15/24 (62%)
Frame = -1
Query: 111 CHFEFNWTFLNSLNFIFFVCDSHN 134
C F +W ++ LN F VC++++
Sbjct: 108 CFFLLSWVLISGLNVCFPVCETYS 37
>BP079801
Length = 387
Score = 25.8 bits (55), Expect = 3.1
Identities = 13/28 (46%), Positives = 15/28 (53%), Gaps = 2/28 (7%)
Frame = +3
Query: 98 LDRKERE--HLLNERCHFEFNWTFLNSL 123
L +KE E H+ N H F W FLN L
Sbjct: 267 LKKKENEINHMNNGFFHISFLWVFLNQL 350
>TC14103 similar to UP|Q84XC6 (Q84XC6) Plasma intrinsic protein 2,2, partial
(94%)
Length = 1199
Score = 25.8 bits (55), Expect = 3.1
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = +2
Query: 119 FLNSLNFIFFVCDSHNHHPYHW 140
F S N++ V S HHP+HW
Sbjct: 695 FGTSSNWVCSVHGSLGHHPHHW 760
>TC12135 similar to GB|AAP37803.1|30725562|BT008444 At4g35750 {Arabidopsis
thaliana;}, partial (78%)
Length = 562
Score = 25.8 bits (55), Expect = 3.1
Identities = 15/54 (27%), Positives = 27/54 (49%)
Frame = -3
Query: 29 IPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDS 82
+P + + E + EE R E R + I+GF+ LLD + + D+++S
Sbjct: 485 VPQLPVQPGVEEEAAEGGEEEARLEARVNE---IDGFQILLDVGRDRVVDRSES 333
>TC9848 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin
PP2-like protein, partial (19%)
Length = 394
Score = 25.8 bits (55), Expect = 3.1
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 116 NWTFLNSLNFIFFVCDSHNHH 136
NWT L + N IFF+ +S+ H
Sbjct: 296 NWTSLQAKNLIFFLQESYR*H 358
>BP052246
Length = 527
Score = 25.4 bits (54), Expect = 4.1
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 8/66 (12%)
Frame = +3
Query: 71 EASEDIDDKTDSHTFRKKWGNDPRFEALDRKEREHLLNERCHFE--------FNWTFLNS 122
E S +D K S T RK W + + E+L + HL C+F F+ +
Sbjct: 48 EQSSHVDKK--SQTARKMW*KNGQGESLIKTMCFHLFCLLCYFSIIDY*PFVFSVDCVRE 221
Query: 123 LNFIFF 128
LN I+F
Sbjct: 222 LNIIYF 239
>BP033771
Length = 513
Score = 25.4 bits (54), Expect = 4.1
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = -2
Query: 38 LFEHYVKNRAEEERKEKRAAQKAAIEGFKQLLDEASEDID 77
L + K+RAE+ EK+ A++ + L A +DID
Sbjct: 377 LIQEAEKHRAEDRMYEKKVEAMNALDDYVYKLRNAIKDID 258
>TC16522 similar to UP|Q84PB7 (Q84PB7) Inositol phosphatase-like protein,
partial (44%)
Length = 642
Score = 25.4 bits (54), Expect = 4.1
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = +2
Query: 21 VFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQK 59
V+D + PS R ++F+ Y+ E+ + ++ AQK
Sbjct: 437 VYDKLMEGYPSDEDRDAIFQAYINALKEDPEQYRKDAQK 553
>TC11653 similar to UP|O33616 (O33616) Histidine kinase, partial (5%)
Length = 471
Score = 25.0 bits (53), Expect = 5.3
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +1
Query: 112 HFEFNWTFLNSLNFIFFVCDSHNHH 136
H + TFL+S++ FF HN H
Sbjct: 22 HLIISLTFLSSISISFFSITHHNSH 96
>TC8642 similar to UP|Q9ASR5 (Q9ASR5) AT4g35920/F4B14_190, partial (19%)
Length = 651
Score = 25.0 bits (53), Expect = 5.3
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 111 CHFEFNWTFLNSLNFI 126
CH F WTF NSL+ +
Sbjct: 580 CHGSFCWTFSNSLDTV 627
>TC14611 similar to UP|Q8LPB2 (Q8LPB2) Glycine-rich RNA binding protein,
partial (20%)
Length = 760
Score = 25.0 bits (53), Expect = 5.3
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +3
Query: 75 DIDDKTDSHTFRKKWGNDPRFEALDRKEREHLLNERCHFE 114
D DD + RKK+G+D + +RK+ H + R +++
Sbjct: 261 DDDDDDNEGYARKKYGDDGSDDDDERKKHHHKHHHRKNYD 380
>AV427932
Length = 425
Score = 24.6 bits (52), Expect = 7.0
Identities = 13/38 (34%), Positives = 19/38 (49%)
Frame = +3
Query: 98 LDRKEREHLLNERCHFEFNWTFLNSLNFIFFVCDSHNH 135
+DR+ H F FN+ F+ NF F+ +S NH
Sbjct: 48 VDRETLLHTQIGAVFFFFNFIFIVIFNFHRFIPNSFNH 161
>AV780676
Length = 526
Score = 24.6 bits (52), Expect = 7.0
Identities = 8/22 (36%), Positives = 13/22 (58%)
Frame = +3
Query: 96 EALDRKEREHLLNERCHFEFNW 117
+ +D ++ E L + RCHF W
Sbjct: 279 QGIDGEDLERLRDRRCHFTRRW 344
>TC12733 similar to UP|Q9SAS9 (Q9SAS9) Heat shock protein 90-2 (Fragment),
partial (6%)
Length = 980
Score = 24.6 bits (52), Expect = 7.0
Identities = 10/17 (58%), Positives = 12/17 (69%)
Frame = +2
Query: 87 KKWGNDPRFEALDRKER 103
K G+DPRF A+DR R
Sbjct: 305 KTHGSDPRFVAIDRHNR 355
>TC18671 UP|Q40198 (Q40198) RAB11H, complete
Length = 1181
Score = 24.6 bits (52), Expect = 7.0
Identities = 18/55 (32%), Positives = 24/55 (42%), Gaps = 4/55 (7%)
Frame = +3
Query: 74 EDIDDKTDSHTFRKKWGNDPRFEALDRKEREH----LLNERCHFEFNWTFLNSLN 124
+++ D TD++ GN L E LLNER HF + L SLN
Sbjct: 528 KELRDHTDTNIVVMLVGNKADLRHLRAVSTEDSSGFLLNERTHFFMETSALESLN 692
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.137 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,146,104
Number of Sequences: 28460
Number of extensions: 44550
Number of successful extensions: 363
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 363
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 363
length of query: 146
length of database: 4,897,600
effective HSP length: 82
effective length of query: 64
effective length of database: 2,563,880
effective search space: 164088320
effective search space used: 164088320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC144765.19