BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144759.6 + phase: 0
(579 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17147 similar to UP|Q8LP14 (Q8LP14) Nine-cis-epoxycarotenoid d... 202 9e-53
AV419581 107 4e-24
AV422261 99 2e-21
AV410275 93 1e-19
BF177847 89 3e-18
BP084864 69 2e-12
TC12686 similar to UP|Q9FS24 (Q9FS24) Neoxanthin cleavage enzyme... 63 1e-10
TC19935 similar to UP|Q9FS24 (Q9FS24) Neoxanthin cleavage enzyme... 58 4e-09
BU494410 50 5e-08
TC13829 similar to UP|P93321 (P93321) Cdc2MsD protein, partial (... 31 0.48
AW719741 28 4.1
TC12176 weakly similar to UP|AAQ09151 (AAQ09151) NADH dehydrogen... 28 5.3
AV771262 28 5.3
BP072590 27 7.0
TC10908 weakly similar to PIR|T50493|T50493 syntaxin-like protei... 27 7.0
TC14227 homologue to UP|Q9LLR4 (Q9LLR4) Sec61p, partial (53%) 27 9.1
TC9071 similar to UP|Q9LT85 (Q9LT85) Similarity to receptor prot... 27 9.1
TC16424 similar to UP|Q9FZF1 (Q9FZF1) T2E6.18, partial (39%) 27 9.1
>TC17147 similar to UP|Q8LP14 (Q8LP14) Nine-cis-epoxycarotenoid
dioxygenase4, partial (22%)
Length = 663
Score = 202 bits (515), Expect = 9e-53
Identities = 96/126 (76%), Positives = 111/126 (87%), Gaps = 1/126 (0%)
Frame = +3
Query: 455 VINNEFLGKKHRFIYAAIGNPMPKFSGVVKIDVLKGEEVGCRLYGEDCYGGEPFFV-ARE 513
VIN FLG+K RF+YAA+G+PMPK SGVVKIDV+ GE VGCR+YGE CYGGEPFFV A E
Sbjct: 9 VINQRFLGRKSRFVYAAVGDPMPKISGVVKIDVVSGETVGCRMYGEGCYGGEPFFVSAGE 188
Query: 514 DGEEEDDGYLVSYVHDEKKGESRFLVMDAKSPEFEIVAEVKLPRRVPYGFHGLFVKESDI 573
+ EEEDDGYLVSYVHDE KGESRFLVMDA+SPE E+VA VKLPRRVPYGFHGLFV +S++
Sbjct: 189 EVEEEDDGYLVSYVHDEGKGESRFLVMDAQSPELEVVAAVKLPRRVPYGFHGLFVSDSEL 368
Query: 574 TRLSVS 579
++S+S
Sbjct: 369 RKVSLS 386
>AV419581
Length = 412
Score = 107 bits (268), Expect = 4e-24
Identities = 55/133 (41%), Positives = 77/133 (57%), Gaps = 1/133 (0%)
Frame = +1
Query: 186 GYQILPNVFSGFNSLIGLAARGSVAVARVLTGQYNPINGIGLINTSLALFGNRLFALGES 245
G I P + G+A R ++ AR G + +G+G+ N L F N L A+ E
Sbjct: 4 GRPIFPKAIGELHGHSGIA-RLALFYARATLGIVDSSHGMGVANAGLVYFNNHLLAMSED 180
Query: 246 DLPYEIKVTPNGDIQTIGRYDFNGKLFMNMTAHPKIDSDTGECFAYRYGLI-RPFLTYFR 304
DLPY ++V+PNGD+ T+GRY+FN +L M AHPK+D G A Y ++ +P+L YFR
Sbjct: 181 DLPYHVRVSPNGDLTTVGRYNFNDQLKTTMIAHPKVDPVDGTLHALSYDVVQKPYLRYFR 360
Query: 305 FDSNGVKHNDVPV 317
F NGVK DV +
Sbjct: 361 FSENGVKSPDVEI 399
>AV422261
Length = 467
Score = 99.0 bits (245), Expect = 2e-21
Identities = 46/89 (51%), Positives = 61/89 (67%)
Frame = +1
Query: 98 PRHVLSQNFAPVLDELPPTQCKVIKGTLPPSLNGVYIRNGPNPQFLPRGPYHLFDGDGML 157
P H L+ NFAPV DE PPT+ ++G LP LNG ++R GPNP+F P YH FDGDGM+
Sbjct: 133 PHHWLAGNFAPVHDETPPTKNLPVRGFLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMI 312
Query: 158 HAVKITNGKATLCSRYVQTYKYKIENEAG 186
H ++I +GKA+ SR+V+T + K E G
Sbjct: 313 HGLRIKDGKASYVSRFVRTSRLKQEEYFG 399
>AV410275
Length = 431
Score = 93.2 bits (230), Expect = 1e-19
Identities = 60/126 (47%), Positives = 73/126 (57%), Gaps = 17/126 (13%)
Frame = +3
Query: 1 MVPKPIIIIACKQPPTTYIPQFNTASVRTKEKPQTSQPKTLRTTPTKTLLALLTTTTQK- 59
MVPK II + + ++P +SVR +E PQT +T+RTTP+ +L TTTTQ
Sbjct: 78 MVPKSIITTSA----SPHLPLLKISSVRIEENPQT---QTIRTTPSPSLPT--TTTTQNP 230
Query: 60 ----LKNT------------NKTLQTSSISALILNTLDDIINTFIDPPIKPSVDPRHVLS 103
LK T K S+ AL+ N DDIIN FIDPP+KPSVDPRHVLS
Sbjct: 231 SKPLLKKTPPPPPSTPFNAKRKPQPPPSVPALLFNAFDDIINNFIDPPLKPSVDPRHVLS 410
Query: 104 QNFAPV 109
QNFAPV
Sbjct: 411 QNFAPV 428
>BF177847
Length = 444
Score = 88.6 bits (218), Expect = 3e-18
Identities = 49/131 (37%), Positives = 78/131 (59%), Gaps = 5/131 (3%)
Frame = +3
Query: 323 PSFLHDFAITKKYALFADIQLEINPLDMIFGGSPVRS-DPSKVPRIGILPRYADNESKMK 381
P +HDFAIT+ YA+F D+ L P +M+ + + S D +K R G+LPRYA ++ +++
Sbjct: 15 PIMMHDFAITENYAIFMDLPLYFRPKEMVKNKTLIFSFDSTKKARFGVLPRYAKDDKQIR 194
Query: 382 WFDVPGLNIMHLINAWDEEDGK---TVTIVAPNILCV-EHMMERLELIHEMIEKVRINVE 437
WF++P I H NAW+EED T + P + V + E+LE + ++R N++
Sbjct: 195 WFELPNCFIFHNANAWEEEDEVVLITCRLENPELDNVGGAVKEKLESFSNELYEMRFNMK 374
Query: 438 TGIVTRQPLSA 448
TG ++Q LSA
Sbjct: 375 TGEASQQKLSA 407
>BP084864
Length = 502
Score = 68.9 bits (167), Expect = 2e-12
Identities = 39/129 (30%), Positives = 66/129 (50%), Gaps = 22/129 (17%)
Frame = -1
Query: 459 EFLGKKHRFIY-------AAIGNPMPKFSGVVKIDVLKGE---------------EVGCR 496
+F+G K+R+ Y A+ PK+ G+ K+ + +V
Sbjct: 502 KFIGIKNRYAYTQVVDHAASTTRDRPKYGGLAKLYFEESRAKVPMRDKE*PEEPIKVEYH 323
Query: 497 LYGEDCYGGEPFFVAREDGEEEDDGYLVSYVHDEKKGESRFLVMDAKSPEFEIVAEVKLP 556
+Y ++ + FV +++G EEDDG+++++VH+E S ++D K E V ++ LP
Sbjct: 322 MYEKNKFCTGASFVPKDEGVEEDDGWVITFVHNEDTNTSEVHIIDTKKFSGETVVKITLP 143
Query: 557 RRVPYGFHG 565
RRVPYGFHG
Sbjct: 142 RRVPYGFHG 116
>TC12686 similar to UP|Q9FS24 (Q9FS24) Neoxanthin cleavage enzyme, partial
(18%)
Length = 632
Score = 62.8 bits (151), Expect = 1e-10
Identities = 45/138 (32%), Positives = 66/138 (47%), Gaps = 2/138 (1%)
Frame = +3
Query: 5 PIIIIACKQPPTTYIPQFNTAS--VRTKEKPQTSQPKTLRTTPTKTLLALLTTTTQKLKN 62
P I I K+ T+ I T S ++T P+ QP TT TKT T + L
Sbjct: 225 PKISITFKKGSTSTISSNKTVSCSLQTLHFPKKYQPAKTATTTTKTATPAKENTAKSLPQ 404
Query: 63 TNKTLQTSSISALILNTLDDIINTFIDPPIKPSVDPRHVLSQNFAPVLDELPPTQCKVIK 122
LQ ++ +AL + ++ P+ + DPR ++ NFAPV E P T +
Sbjct: 405 NWNLLQKAAATALD-HVESALVRRERQTPLPKTADPRVQIAGNFAPV-PEHPVTHSLPVT 578
Query: 123 GTLPPSLNGVYIRNGPNP 140
G +P ++GVY+RNG NP
Sbjct: 579 GKIPKCIDGVYLRNGANP 632
Score = 30.8 bits (68), Expect = 0.63
Identities = 19/47 (40%), Positives = 28/47 (59%), Gaps = 4/47 (8%)
Frame = +3
Query: 37 QPKTLRTTPTKTLLALLTTTTQKLKNTN----KTLQTSSISALILNT 79
QP+ L+ + T T LALLTT T+ + + +T+ TS IS +NT
Sbjct: 27 QPQFLQISQTHTFLALLTTHTK*ISTHSLQK*RTMATSGISNTWINT 167
>TC19935 similar to UP|Q9FS24 (Q9FS24) Neoxanthin cleavage enzyme, partial
(9%)
Length = 567
Score = 58.2 bits (139), Expect = 4e-09
Identities = 24/56 (42%), Positives = 41/56 (72%)
Frame = +2
Query: 520 DGYLVSYVHDEKKGESRFLVMDAKSPEFEIVAEVKLPRRVPYGFHGLFVKESDITR 575
DGY++++VHDEK+ +S +++A + + E A ++LP RVPYGFHG F++ D+ +
Sbjct: 2 DGYILTFVHDEKEWKSELQIVNAMNLKLE--ATIQLPSRVPYGFHGTFIESEDLKK 163
>BU494410
Length = 310
Score = 50.1 bits (118), Expect(2) = 5e-08
Identities = 23/48 (47%), Positives = 31/48 (63%)
Frame = +1
Query: 528 HDEKKGESRFLVMDAKSPEFEIVAEVKLPRRVPYGFHGLFVKESDITR 575
HDE G+S V+DAK+ + +A V+LP RVPYGFH FV E + +
Sbjct: 97 HDENTGKSFVHVIDAKTMSADPIAFVELPHRVPYGFHAFFVTEDQLQK 240
Score = 23.9 bits (50), Expect(2) = 5e-08
Identities = 11/26 (42%), Positives = 15/26 (57%), Gaps = 2/26 (7%)
Frame = +3
Query: 503 YGGEPFFVAREDG--EEEDDGYLVSY 526
+ E +V R G EEDDGYL+ +
Sbjct: 15 FASEAVYVPRVPGTDSEEDDGYLILF 92
>TC13829 similar to UP|P93321 (P93321) Cdc2MsD protein, partial (43%)
Length = 515
Score = 31.2 bits (69), Expect = 0.48
Identities = 20/70 (28%), Positives = 31/70 (43%)
Frame = +2
Query: 10 ACKQPPTTYIPQFNTASVRTKEKPQTSQPKTLRTTPTKTLLALLTTTTQKLKNTNKTLQT 69
+ K P + P +T+S +K T PKT TTP TT + N + T +
Sbjct: 227 SAKSPSSRCSPNPSTSSASSKSSTSTKPPKTTTTTPPPP-----PTTLLRRLNPSSTSSS 391
Query: 70 SSISALILNT 79
S+ + + NT
Sbjct: 392 STSTPISRNT 421
>AW719741
Length = 413
Score = 28.1 bits (61), Expect = 4.1
Identities = 15/51 (29%), Positives = 24/51 (46%), Gaps = 1/51 (1%)
Frame = +2
Query: 91 PIKPSVDPRHVLSQNFAPVLDELPPTQCKVIKGTLPPSLNGVYIRNG-PNP 140
P++ +V+ + Q P L + PT C V G+ PP + G P+P
Sbjct: 200 PLESTVNATRL*QQTLQPPLTHVTPTACSVSGGSPPPRTATSSVSRGLPSP 352
>TC12176 weakly similar to UP|AAQ09151 (AAQ09151) NADH dehydrogenase subunit
2, partial (6%)
Length = 478
Score = 27.7 bits (60), Expect = 5.3
Identities = 14/38 (36%), Positives = 21/38 (54%)
Frame = -3
Query: 93 KPSVDPRHVLSQNFAPVLDELPPTQCKVIKGTLPPSLN 130
KP DP+H+LS+ + P T+ K IKG ++N
Sbjct: 275 KPDQDPKHILSKELYKI--RYPGTKPKGIKGNPSTAIN 168
>AV771262
Length = 360
Score = 27.7 bits (60), Expect = 5.3
Identities = 12/37 (32%), Positives = 20/37 (53%)
Frame = -3
Query: 311 KHNDVPVFSMKRPSFLHDFAITKKYALFADIQLEINP 347
+H+ V++ PS LH+F + + LF I +NP
Sbjct: 196 RHHSYTVYN*HYPSTLHNFCMCSLFILFLFIPESVNP 86
>BP072590
Length = 493
Score = 27.3 bits (59), Expect = 7.0
Identities = 17/41 (41%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = +1
Query: 111 DELPPTQCKVIKGTLP-PSLNGVYIRNGPNPQFLPRGPYHL 150
D LP + K LP P L+G + PQ LPR P HL
Sbjct: 367 DHLPVSLFSPAKRHLPDPQLHGCGMLTFTIPQSLPRAPIHL 489
>TC10908 weakly similar to PIR|T50493|T50493 syntaxin-like protein -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(34%)
Length = 600
Score = 27.3 bits (59), Expect = 7.0
Identities = 11/29 (37%), Positives = 17/29 (57%)
Frame = -2
Query: 272 FMNMTAHPKIDSDTGECFAYRYGLIRPFL 300
F+N+ HP +D+ G CF R+ + FL
Sbjct: 428 FINIFLHPLLDTFHGRCFCNRF*FLISFL 342
>TC14227 homologue to UP|Q9LLR4 (Q9LLR4) Sec61p, partial (53%)
Length = 880
Score = 26.9 bits (58), Expect = 9.1
Identities = 13/42 (30%), Positives = 22/42 (51%)
Frame = +2
Query: 193 VFSGFNSLIGLAARGSVAVARVLTGQYNPINGIGLINTSLAL 234
+ +G L+G+ AVA VL+G Y + +G+ N L +
Sbjct: 470 LLNGAQKLLGILIAVGEAVAYVLSGMYGSVGQLGVGNAILII 595
>TC9071 similar to UP|Q9LT85 (Q9LT85) Similarity to receptor protein
kinase, partial (15%)
Length = 872
Score = 26.9 bits (58), Expect = 9.1
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +2
Query: 14 PPTTYIPQFNTASVRTKEKPQTSQPKTLRTTPTKT 48
PP ++P N ++V TK +S TTPT T
Sbjct: 278 PPAAHLPHPNHSAV*TKLAASSSNSPPTSTTPTAT 382
>TC16424 similar to UP|Q9FZF1 (Q9FZF1) T2E6.18, partial (39%)
Length = 537
Score = 26.9 bits (58), Expect = 9.1
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 14 PPTTYIPQFNTASVRTKEKPQTSQPKT 40
PP++ P T+SVRT+ P S+P +
Sbjct: 268 PPSSSSPLPRTSSVRTRTSPNASRPSS 348
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.140 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,355,021
Number of Sequences: 28460
Number of extensions: 134584
Number of successful extensions: 629
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 620
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 623
length of query: 579
length of database: 4,897,600
effective HSP length: 95
effective length of query: 484
effective length of database: 2,193,900
effective search space: 1061847600
effective search space used: 1061847600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144759.6