BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144731.8 - phase: 0
(517 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8497 similar to UP|PDA6_MEDSA (P38661) Probable protein disulf... 61 4e-10
TC7923 similar to UP|PDI_MEDSA (P29828) Protein disulfide isomer... 53 1e-07
BP036717 40 0.001
CB827734 38 0.003
TC16191 similar to GB|AAO39946.1|28372928|BT003718 At1g49880 {Ar... 38 0.003
TC17161 homologue to UP|THIF_PEA (P29450) Thioredoxin F-type, ch... 35 0.039
AU252164 32 0.19
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 32 0.25
TC15929 similar to UP|THM4_ARATH (Q9SEU6) Thioredoxin M-type 4, ... 31 0.43
TC14552 similar to UP|THM4_ARATH (Q9SEU6) Thioredoxin M-type 4, ... 30 0.73
TC18212 30 1.2
TC19338 similar to GB|AAC49358.1|1388088|PSU35831 thioredoxin m ... 29 1.6
TC17672 similar to UP|Q93X83 (Q93X83) Hsp70 interacting protein/... 29 2.1
BP043095 29 2.1
TC11260 homologue to UP|Q9ASY1 (Q9ASY1) AT3g03250/T17B22_6, part... 29 2.1
AV426732 28 2.8
TC17116 weakly similar to UP|THM3_ARATH (Q9SEU7) Thioredoxin M-t... 28 2.8
AV423063 28 2.8
AV424143 28 2.8
TC10515 28 2.8
>TC8497 similar to UP|PDA6_MEDSA (P38661) Probable protein disulfide
isomerase A6 precursor (P5) , partial (95%)
Length = 1533
Score = 61.2 bits (147), Expect = 4e-10
Identities = 42/132 (31%), Positives = 61/132 (45%)
Frame = +2
Query: 44 TTDHDYAVELNATNFDAVLKDTPATFAVVEFFANWCPACRNYKPHYEKVAKLFNGPDAVH 103
T D V L+ NF+ + A+VEF+A WC C+ P YEK+ F +V
Sbjct: 179 TASADDVVVLSEDNFEKEVGQDKG--ALVEFYAPWCGHCKKLAPEYEKLGGSFKKAKSV- 349
Query: 104 PGIILLTRVDCASKINIKLCDKFSVGHYPMLFWGPPPKFVGGSWEPKQEKSDIHVIDDAR 163
L+ +VDC ++ C K+ V YP L W P GS EPK+ + R
Sbjct: 350 ----LIAKVDCDEHKSV--CSKYGVSGYPTLQWFPK-----GSLEPKK-------YEGPR 475
Query: 164 TADRLLNWINKQ 175
TA+ L ++N +
Sbjct: 476 TAEALAEFVNTE 511
Score = 45.4 bits (106), Expect = 2e-05
Identities = 33/105 (31%), Positives = 50/105 (47%)
Frame = +2
Query: 51 VELNATNFDAVLKDTPATFAVVEFFANWCPACRNYKPHYEKVAKLFNGPDAVHPGIILLT 110
V L A NF+ V+ D +VEF+A WC C++ P YEKVA F G +++
Sbjct: 554 VVLTAENFNEVVLDETKD-VLVEFYAPWCGHCKSLAPTYEKVAAAFK-----LDGDVVIA 715
Query: 111 RVDCASKINIKLCDKFSVGHYPMLFWGPPPKFVGGSWEPKQEKSD 155
+D A K L +K+ V +P L + P G + ++ D
Sbjct: 716 NLD-ADKYR-DLAEKYEVSGFPTLKFFPKGNKAGEEYGGGRDLDD 844
>TC7923 similar to UP|PDI_MEDSA (P29828) Protein disulfide isomerase
precursor (PDI) , partial (89%)
Length = 1861
Score = 53.1 bits (126), Expect = 1e-07
Identities = 35/128 (27%), Positives = 61/128 (47%)
Frame = +2
Query: 48 DYAVELNATNFDAVLKDTPATFAVVEFFANWCPACRNYKPHYEKVAKLFNGPDAVHPGII 107
++ + L+ +NF ++ + F VVEF+A WC C+N P YEK A + + H I
Sbjct: 143 EFVLTLDNSNFSEIV--SKHDFIVVEFYAPWCGHCKNLAPEYEKAAAVLSS----HDPPI 304
Query: 108 LLTRVDCASKINIKLCDKFSVGHYPMLFWGPPPKFVGGSWEPKQEKSDIHVIDDARTADR 167
+L +VD + N L ++ V +P + K + + QE R AD
Sbjct: 305 VLAKVDANEENNKDLASQYEVRGFPTI------KILRNGGKDSQE------YKGPREADG 448
Query: 168 LLNWINKQ 175
+++++ KQ
Sbjct: 449 IVDYLKKQ 472
Score = 31.6 bits (70), Expect = 0.33
Identities = 27/121 (22%), Positives = 55/121 (45%), Gaps = 1/121 (0%)
Frame = +2
Query: 71 VVEFFANWCPACRNYKPHYEKVAKLFNGPDAVHPGIILLTRVDCASKINIKLCDKFSVGH 130
++EF+A WC C++ P ++VA + + I++ + D + N + F V
Sbjct: 1241 LLEFYAPWCGHCKSLAPILDEVAVSYQ-----NDADIVIAKFDATA--NDVPTESFEVQG 1399
Query: 131 YPMLFWGPPPKFVGGSWEPKQEKSDIHVIDDARTADRLLNWI-NKQMSSSFGLDDQKFQN 189
YP L+ F+ S I D RT + ++ +I N + S +++++ ++
Sbjct: 1400 YPTLY------FISSS-------GKISPYDGGRTKEDIIQFIENSRDKKSTQIEEEQGKD 1540
Query: 190 E 190
E
Sbjct: 1541 E 1543
>BP036717
Length = 477
Score = 40.0 bits (92), Expect = 0.001
Identities = 34/137 (24%), Positives = 61/137 (43%)
Frame = +1
Query: 40 DQTGTTDHDYAVELNATNFDAVLKDTPATFAVVEFFANWCPACRNYKPHYEKVAKLFNGP 99
++ D D V L NF V+++ F +VEF+A WC C++ P Y A
Sbjct: 124 EEPAVDDKDVVV-LIERNFTTVVENN--RFVMVEFYAPWCGHCQSLAPEYAAAA-----T 279
Query: 100 DAVHPGIILLTRVDCASKINIKLCDKFSVGHYPMLFWGPPPKFVGGSWEPKQEKSDIHVI 159
+ + G++ L +VD + + L ++ V +P + + F+ G +P
Sbjct: 280 ELKNDGVV-LAKVDATQEND--LAHEYDVQGFPTVSF-----FIDGVHKP---------Y 408
Query: 160 DDARTADRLLNWINKQM 176
RT D ++ WI K++
Sbjct: 409 TGQRTKDAIVTWIRKKI 459
>CB827734
Length = 556
Score = 38.1 bits (87), Expect = 0.003
Identities = 18/48 (37%), Positives = 28/48 (57%)
Frame = +1
Query: 51 VELNATNFDAVLKDTPATFAVVEFFANWCPACRNYKPHYEKVAKLFNG 98
V+LN +NF + + ++ +VEFFA WC C+ P +EK A + G
Sbjct: 139 VQLNPSNFKSKVLNSKGV-VLVEFFAPWCGHCKALTPIWEKAATVLKG 279
>TC16191 similar to GB|AAO39946.1|28372928|BT003718 At1g49880 {Arabidopsis
thaliana;}, partial (60%)
Length = 587
Score = 38.1 bits (87), Expect = 0.003
Identities = 20/77 (25%), Positives = 32/77 (40%), Gaps = 3/77 (3%)
Frame = +2
Query: 315 WVLLHSLSVRIEDGESQFTFNAICDFVH---NFFVCEECRQHFYDMCSSVSTPFNKARDF 371
W LH+L+ + D ++ + + V + C+EC HF ++ + F
Sbjct: 92 WTFLHTLAAQYPDNPTRQQKKDVKELVQILSRLYPCKECADHFKEVLRANPVQAESHAQF 271
Query: 372 VLWLWSSHNKVNERLSK 388
WL HN VN L K
Sbjct: 272 SHWLCHVHNVVNRSLGK 322
Score = 27.7 bits (60), Expect = 4.7
Identities = 20/66 (30%), Positives = 32/66 (48%), Gaps = 7/66 (10%)
Frame = +2
Query: 218 DIILENKMIKPETRASLVK----FLQVLTAHHP---SRRCRKGAGDLLVSFADLYPTDFW 270
D IL+ K P T+ L + FL L A +P +R+ +K +L+ + LYP
Sbjct: 29 DNILQGKSAPPVTKEELGRATWTFLHTLAAQYPDNPTRQQKKDVKELVQILSRLYPCKEC 208
Query: 271 SSHKQE 276
+ H +E
Sbjct: 209 ADHFKE 226
>TC17161 homologue to UP|THIF_PEA (P29450) Thioredoxin F-type, chloroplast
precursor (TRX-F), partial (72%)
Length = 850
Score = 34.7 bits (78), Expect = 0.039
Identities = 23/85 (27%), Positives = 40/85 (47%), Gaps = 6/85 (7%)
Frame = +2
Query: 16 SSSATSSSSSSFPGRSILRE----VHDNDQTG--TTDHDYAVELNATNFDAVLKDTPATF 69
SSS++SS+SS + S+ R V + +T T E+N F ++
Sbjct: 137 SSSSSSSASSVYRSVSLRRSGSVAVRSSLETAGPTVTVGKVTEVNKDTFWPIVNAAGDKT 316
Query: 70 AVVEFFANWCPACRNYKPHYEKVAK 94
V++ F WC C+ P Y+++A+
Sbjct: 317 VVLDMFTQWCGPCKVIAPKYQELAE 391
>AU252164
Length = 350
Score = 32.3 bits (72), Expect = 0.19
Identities = 19/82 (23%), Positives = 35/82 (42%)
Frame = +2
Query: 51 VELNATNFDAVLKDTPATFAVVEFFANWCPACRNYKPHYEKVAKLFNGPDAVHPGIILLT 110
+ L + F +K+ + V+F WC C+N ++ + G D + G
Sbjct: 104 ITLTSDTFSDKIKEKDTAW-FVKFCVPWCKHCKNLGSLWDDLGTAMEGEDEIXIG----- 265
Query: 111 RVDCASKINIKLCDKFSVGHYP 132
VDC+++ + C K + YP
Sbjct: 266 EVDCSTEKPV--CSKVDIHSYP 325
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 32.0 bits (71), Expect = 0.25
Identities = 17/30 (56%), Positives = 21/30 (69%)
Frame = +1
Query: 2 NLLYFALFLCTISVSSSATSSSSSSFPGRS 31
N++YF LFL S SSS++SSSSSS S
Sbjct: 235 NVVYFFLFLGGCSASSSSSSSSSSSLSSSS 324
>TC15929 similar to UP|THM4_ARATH (Q9SEU6) Thioredoxin M-type 4, chloroplast
precursor (TRX-M4), partial (49%)
Length = 1406
Score = 31.2 bits (69), Expect = 0.43
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = +1
Query: 68 TFAVVEFFANWCPACRNYKPHYEKVAKLFNG 98
T +VEF+A WC CR P +++AK + G
Sbjct: 355 TAVLVEFWAPWCGPCRMIHPVIDELAKEYAG 447
>TC14552 similar to UP|THM4_ARATH (Q9SEU6) Thioredoxin M-type 4, chloroplast
precursor (TRX-M4), partial (55%)
Length = 1016
Score = 30.4 bits (67), Expect = 0.73
Identities = 12/28 (42%), Positives = 18/28 (63%)
Frame = +1
Query: 71 VVEFFANWCPACRNYKPHYEKVAKLFNG 98
+VEF+A WC CR P +++AK + G
Sbjct: 361 LVEFWAPWCGPCRMIHPIIDELAKQYAG 444
>TC18212
Length = 750
Score = 29.6 bits (65), Expect = 1.2
Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Frame = +2
Query: 384 ERLSKEEASLGTGDPKFPKTIWPTKQLCPSCYLGHDQ-KSNKIEW 427
E++ + L PK P T WP +L CYLG + S+ I W
Sbjct: 101 EQIRMKSFYLKPAVPKRPSTSWPNTELEGCCYLGESKFYSSGIGW 235
>TC19338 similar to GB|AAC49358.1|1388088|PSU35831 thioredoxin m {Pisum
sativum;} , partial (65%)
Length = 476
Score = 29.3 bits (64), Expect = 1.6
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +2
Query: 71 VVEFFANWCPACRNYKPHYEKVAKLFNGPDAVH 103
+V+F+A WC CR P +++AK + G A +
Sbjct: 92 LVDFWAPWCGPCRMIAPLIDELAKEYAGKIACY 190
>TC17672 similar to UP|Q93X83 (Q93X83) Hsp70 interacting protein/thioredoxin
chimera, partial (33%)
Length = 709
Score = 28.9 bits (63), Expect = 2.1
Identities = 17/57 (29%), Positives = 26/57 (44%)
Frame = +1
Query: 70 AVVEFFANWCPACRNYKPHYEKVAKLFNGPDAVHPGIILLTRVDCASKINIKLCDKF 126
A++ F A WC CR P Y +A+ + P V + + D A++ NI F
Sbjct: 160 AILYFTATWCGPCRFISPIYTSLAEKY--PKVVFLKVDIDEARDVAARWNISSVPSF 324
>BP043095
Length = 396
Score = 28.9 bits (63), Expect = 2.1
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 71 VVEFFANWCPACRNYKPHYEKVAKLF 96
VV+F A+WC CR P+ ++AK +
Sbjct: 154 VVDFTASWCGPCRFIAPYLGELAKKY 231
>TC11260 homologue to UP|Q9ASY1 (Q9ASY1) AT3g03250/T17B22_6, partial (7%)
Length = 482
Score = 28.9 bits (63), Expect = 2.1
Identities = 14/48 (29%), Positives = 25/48 (51%)
Frame = -2
Query: 343 NFFVCEECRQHFYDMCSSVSTPFNKARDFVLWLWSSHNKVNERLSKEE 390
NFF + F+ C S F+ + +L+LW SH+K ++R + +
Sbjct: 175 NFFTSIQYSFLFWIKCFRTSISFS*NKFQLLFLWDSHHKTSKRYKERK 32
>AV426732
Length = 238
Score = 28.5 bits (62), Expect = 2.8
Identities = 13/35 (37%), Positives = 21/35 (59%)
Frame = +3
Query: 63 KDTPATFAVVEFFANWCPACRNYKPHYEKVAKLFN 97
KD+ A V++F A+WC CR +P +A+ F+
Sbjct: 117 KDSSALL-VIDFSASWCGPCRFIEPAIHAMAEKFS 218
>TC17116 weakly similar to UP|THM3_ARATH (Q9SEU7) Thioredoxin M-type 3,
chloroplast precursor (TRX-M3), partial (55%)
Length = 798
Score = 28.5 bits (62), Expect = 2.8
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 71 VVEFFANWCPACRNYKPHYEKVAKLFNG 98
+VEF+A+WC CR + +AK + G
Sbjct: 228 LVEFYASWCGPCRMVHRVIDDIAKEYAG 311
>AV423063
Length = 463
Score = 28.5 bits (62), Expect = 2.8
Identities = 22/91 (24%), Positives = 40/91 (43%), Gaps = 1/91 (1%)
Frame = +2
Query: 7 ALFLCTISVSSSATSSSSSSFPGRSILREVHDNDQTGTTDHDYAVELNATNFDAVLKDTP 66
+LFL I+ S SSS+ S S L + T + ++++ + VL
Sbjct: 32 SLFLFFITCLSFLQSSSAPSPSSASFLFALQSQCSLAITPNP-PLQVDGNFVERVLSGRK 208
Query: 67 AT-FAVVEFFANWCPACRNYKPHYEKVAKLF 96
+ + F+A+WCP R +E ++ +F
Sbjct: 209 RVGYISILFYASWCPFSRRMLSEFEILSSMF 301
>AV424143
Length = 285
Score = 28.5 bits (62), Expect = 2.8
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +1
Query: 68 TFAVVEFFANWCPACR 83
T VV+FFA WC CR
Sbjct: 238 TLVVVDFFATWCGPCR 285
>TC10515
Length = 802
Score = 28.5 bits (62), Expect = 2.8
Identities = 12/19 (63%), Positives = 14/19 (73%), Gaps = 3/19 (15%)
Frame = -3
Query: 396 GDPKFP---KTIWPTKQLC 411
GDP FP K IWP+K+LC
Sbjct: 380 GDPVFP*SIKEIWPSKKLC 324
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,217,322
Number of Sequences: 28460
Number of extensions: 184423
Number of successful extensions: 1285
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 1169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1259
length of query: 517
length of database: 4,897,600
effective HSP length: 94
effective length of query: 423
effective length of database: 2,222,360
effective search space: 940058280
effective search space used: 940058280
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC144731.8