BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144731.14 + phase: 0 /pseudo
(1343 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP063694 101 7e-22
BP033602 82 4e-16
AV766088 75 9e-14
BP081717 47 2e-05
AV427921 44 1e-04
BP063902 44 1e-04
BP058111 41 0.001
BP043850 41 0.001
AV427609 40 0.002
BI418295 40 0.002
BP054351 39 0.007
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 38 0.009
AV779281 38 0.009
AU088980 37 0.016
BP054557 37 0.021
TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 37 0.021
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 36 0.036
AV764934 35 0.061
BP066067 35 0.061
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 35 0.10
>BP063694
Length = 511
Score = 101 bits (252), Expect = 7e-22
Identities = 50/122 (40%), Positives = 72/122 (58%)
Frame = -2
Query: 456 SYEDWHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASISCPVCKLAKSKTLPFPSGAHRAS 515
S+E WH +LGH N ++ L K G L ++ I C C++AKSK L F RAS
Sbjct: 369 SFELWHSRLGHVNFDIIKQLHKHGCLDVSSIL-PKPICCTSCQMAKSKRLVFHDNNKRAS 193
Query: 516 NCFEMIHSDVWGMSPIASHAHYKYFVTFIDDYSRFTWIYFLRSKSEVFSMFKKFLTYVET 575
++IH D+ G SP+AS + YFV F+DD+SRFTW Y L+ KS+ + +F ++E
Sbjct: 192 AVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFMEN 13
Query: 576 QF 577
+F
Sbjct: 12 RF 7
>BP033602
Length = 533
Score = 82.4 bits (202), Expect = 4e-16
Identities = 43/101 (42%), Positives = 60/101 (58%)
Frame = +3
Query: 448 LACNNVLNSYEDWHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASISCPVCKLAKSKTLPF 507
++C +V + WH +LGHPN L HLF K V C+ S+ C C LAK++ P+
Sbjct: 243 MSCTSVRDQIMLWHNRLGHPNFQYLRHLFPDLF---KNVNCS-SLECESCVLAKNQRAPY 410
Query: 508 PSGAHRASNCFEMIHSDVWGMSPIASHAHYKYFVTFIDDYS 548
S + AS F +IHSDVWG S I + ++FVTFIDD++
Sbjct: 411 YSQPYHASRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>AV766088
Length = 501
Score = 74.7 bits (182), Expect = 9e-14
Identities = 37/104 (35%), Positives = 60/104 (57%)
Frame = -3
Query: 460 WHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASISCPVCKLAKSKTLPFPSGAHRASNCFE 519
WH +LGHP+S+ L +L + + + S C C K LPF S + F+
Sbjct: 304 WHSRLGHPSSSALRYLRSNKFISYE--LLNYSPVCESCVFGKHVRLPFVSSNNVTVMPFD 131
Query: 520 MIHSDVWGMSPIASHAHYKYFVTFIDDYSRFTWIYFLRSKSEVF 563
++HSD+W SP+ S A ++++V F+DD++ F W + L +KS+VF
Sbjct: 130 ILHSDLW-TSPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQVF 2
>BP081717
Length = 291
Score = 47.4 bits (111), Expect = 2e-05
Identities = 23/62 (37%), Positives = 33/62 (53%)
Frame = -1
Query: 457 YEDWHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASISCPVCKLAKSKTLPFPSGAHRASN 516
++ WH +LGHP+S VL H+ K V ++I C C +AK LPFP ++
Sbjct: 174 FDMWHFRLGHPSSKVLQHISKV----FPYVKLHSNIICDFCHMAKQTKLPFPISDTKSKA 7
Query: 517 CF 518
CF
Sbjct: 6 CF 1
>AV427921
Length = 284
Score = 44.3 bits (103), Expect = 1e-04
Identities = 20/37 (54%), Positives = 28/37 (75%)
Frame = +1
Query: 615 TPQQNGLAERKNRHLLDVTRSLLLQASVPPRFWVEAL 651
TPQQNG++ERKNR +L++ RSLL + +P F EA+
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAV 132
>BP063902
Length = 494
Score = 44.3 bits (103), Expect = 1e-04
Identities = 31/92 (33%), Positives = 42/92 (44%), Gaps = 3/92 (3%)
Frame = -3
Query: 423 SGKVIAKGPKVGRLFPLQFISSHLSLACNNVLNSYEDWHRKLGHPNSTVLSHLFKTGLLG 482
+G + + +G L+P+ S LA + WH +LGHP S+ L+H L
Sbjct: 270 TGMPLLRCNSLGDLYPVTRSSPFAGLASSV-------WHHRLGHPASSALNH-----LRN 127
Query: 483 NKQVVCTASIS---CPVCKLAKSKTLPFPSGA 511
NK + C S S C C L K LPF S A
Sbjct: 126 NKLIFCEPSRSSSVCDSCVLGKHVRLPFSSSA 31
Score = 28.5 bits (62), Expect = 7.4
Identities = 18/70 (25%), Positives = 26/70 (36%)
Frame = -2
Query: 280 PTQALHATTSSAAPPTITSASDGGSLQPEMIQQMVLAALSNMGIHGKSSNVSRPWFLDSG 339
P + T+S AP I +A SL P PW++D+G
Sbjct: 412 PRPQAYTATASPAPTDIAAAMHTMSLTPP----------------------DTPWYMDTG 299
Query: 340 ASNHMTGSSE 349
AS+H S +
Sbjct: 298 ASSHTAASQD 269
>BP058111
Length = 570
Score = 41.2 bits (95), Expect = 0.001
Identities = 23/65 (35%), Positives = 33/65 (50%), Gaps = 1/65 (1%)
Frame = -1
Query: 460 WHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASIS-CPVCKLAKSKTLPFPSGAHRASNCF 518
WH +LGH +S L+ L K + C S C C +AK K L FP+ +S F
Sbjct: 183 WHLRLGHTSSKKLAILQKNFPF----ITCKKITSPCDTCHMAKQKKLSFPNSVTLSSEIF 16
Query: 519 EMIHS 523
++IH+
Sbjct: 15 DLIHT 1
>BP043850
Length = 515
Score = 41.2 bits (95), Expect = 0.001
Identities = 25/64 (39%), Positives = 35/64 (54%)
Frame = -2
Query: 1251 FGFVVFWLNLDFLRQSQPHFMLTIRVPSRSLQILFSMSAPSISKLIVIQFVTPMMTD*YP 1310
FG VVF+ N +Q +MLT +VP + QILF+M+ +LIV PM +
Sbjct: 505 FGCVVFFQNSVSYNHNQLRYMLTTQVPFKLQQILFTMNGRGT*RLIVTPSEKPMTAELSH 326
Query: 1311 FLMS 1314
+LMS
Sbjct: 325 YLMS 314
>AV427609
Length = 332
Score = 40.4 bits (93), Expect = 0.002
Identities = 22/92 (23%), Positives = 43/92 (45%), Gaps = 4/92 (4%)
Frame = +1
Query: 29 RMYVKGNKLWSHL---DDVSKAPTEKAALEEWEYKDAQIISWILSSIDPQMINN-LRSFS 84
R++ + +++ H+ D P A E+W DA ++ WI S+I ++ + S
Sbjct: 1 RIHARSHRVLHHIVPTADKPPPPVTDPAYEQWATLDATVLQWIYSTISVDLMTTIMEPGS 180
Query: 85 TAQEMWNYLKRIYNQDNAAKRFQLELEIANYK 116
TA W +L ++ + A+ LE E + +
Sbjct: 181 TAFTAWTHLADLFQDNQNARAVTLEQEFSTVR 276
>BI418295
Length = 523
Score = 40.0 bits (92), Expect = 0.002
Identities = 16/79 (20%), Positives = 40/79 (50%)
Frame = +2
Query: 47 APTEKAALEEWEYKDAQIISWILSSIDPQMINNLRSFSTAQEMWNYLKRIYNQDNAAKRF 106
A TE A W +D+ + +W+L+++ P ++ + + + ++W + + A+
Sbjct: 116 AATENPAYTTWIKQDSMVFTWLLTTLSPFVLPTVINCERSSQVWREILDFFENQCRAQSA 295
Query: 107 QLELEIANYKQGNLYVQEF 125
+L E+ N +G+ + E+
Sbjct: 296 KLRSELKNITKGSKSITEY 352
>BP054351
Length = 431
Score = 38.5 bits (88), Expect = 0.007
Identities = 25/84 (29%), Positives = 40/84 (46%), Gaps = 6/84 (7%)
Frame = -1
Query: 420 EQVSGKVIAKGPKVGRLFPLQFISSHLS------LACNNVLNSYEDWHRKLGHPNSTVLS 473
+++SGK+IA L+ + + + ++C +V + WH +LGHPN L
Sbjct: 245 DRISGKMIASAKMKDGLYYFEDTFRNKAAQGLCGMSCTSVRDQIMLWHNRLGHPNFQYLR 66
Query: 474 HLFKTGLLGNKQVVCTASISCPVC 497
HLF K V C +S+ C C
Sbjct: 65 HLFPDLF---KNVNC-SSLECESC 6
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 38.1 bits (87), Expect = 0.009
Identities = 20/70 (28%), Positives = 31/70 (43%), Gaps = 4/70 (5%)
Frame = +1
Query: 228 GKGRDMRQVQCFTCKQFGHVARSCTA----KFCKYCKQNGHVIFDCPIRPPRRTQYPTQA 283
G+G +CF C GH AR C A C C + GH+ +C P + +++P
Sbjct: 361 GRGPPPGSGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKKLSRHPRSD 540
Query: 284 LHATTSSAAP 293
+ S +P
Sbjct: 541 SRSPVRSRSP 570
>AV779281
Length = 505
Score = 38.1 bits (87), Expect = 0.009
Identities = 23/111 (20%), Positives = 51/111 (45%), Gaps = 11/111 (9%)
Frame = +1
Query: 20 NYPAWEFQFRMYVKGNKLWSHLDDVSKAP-----------TEKAALEEWEYKDAQIISWI 68
N+ +W+ V+ ++ S LD P E A + WE +D+ + +W+
Sbjct: 115 NFLSWKQHVEGIVRTHRWQSFLDHPDVPPRFLSDEDRVNAVENPAYQLWEQQDSALFTWL 294
Query: 69 LSSIDPQMINNLRSFSTAQEMWNYLKRIYNQDNAAKRFQLELEIANYKQGN 119
LSS+ P ++ + + S + ++ + + + A+ QL E+ + +G+
Sbjct: 295 LSSLSPTVLPTVVNCSQSWQVREAVLDFFKAHSFAQSTQLRSELRSIAKGS 447
>AU088980
Length = 360
Score = 37.4 bits (85), Expect = 0.016
Identities = 14/38 (36%), Positives = 24/38 (62%)
Frame = +2
Query: 619 NGLAERKNRHLLDVTRSLLLQASVPPRFWVEALSTVCF 656
N + ERK+RH+L+VTR+L+ + +P W A+ +
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAY 115
>BP054557
Length = 538
Score = 37.0 bits (84), Expect = 0.021
Identities = 47/184 (25%), Positives = 77/184 (41%), Gaps = 19/184 (10%)
Frame = -2
Query: 336 LDSGASNHMTGSSEYLHNLHSYHGNQQIQIADGNKLSITDVGD---INSDF--QDVLVSP 390
LD+GA++H+ + + L H + + +G K I + D + F VL P
Sbjct: 534 LDTGATHHVCHNLDVFQALRQVHP-VLLSMPNGQK-EIAHMADSVYFSEKFFLAXVLYVP 361
Query: 391 GLASNLLSVGQLVDN-NCNVNFSRAGCLVQEQVSGKVIAKGPKVGRLFPL--QFISSHLS 447
NL+SV LV + NC + CL+QE+ + K I + G L+ + + +
Sbjct: 360 SFNFNLISVSALVKSLNCRLVIFDECCLLQERPTLKTIGAAEERGGLYAVIDPAVKAKHP 181
Query: 448 LACNNVLNSYED----------WHRKLGHPNSTVLSHLFKT-GLLGNKQVVCTASISCPV 496
A N+ WH +LGH +S L+ L K + KQ+ + C
Sbjct: 180 PAEGNIKTQVSHFNFCQFDCNLWHLRLGHTSSKKLAILQKNFPFITCKQI----TSPCDT 13
Query: 497 CKLA 500
C +A
Sbjct: 12 CHMA 1
>TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (9%)
Length = 450
Score = 37.0 bits (84), Expect = 0.021
Identities = 14/37 (37%), Positives = 20/37 (53%), Gaps = 2/37 (5%)
Frame = +3
Query: 236 VQCFTCKQFGHVARSCTA--KFCKYCKQNGHVIFDCP 270
V C C+Q GH++R C C C GH+ ++CP
Sbjct: 3 VVCRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYECP 113
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 36.2 bits (82), Expect = 0.036
Identities = 15/34 (44%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = +2
Query: 238 CFTCKQFGHVARSCTAK-FCKYCKQNGHVIFDCP 270
C C GH+A CT K C CK+ GH+ CP
Sbjct: 401 CHNCGLPGHIASECTTKSLCWNCKEPGHMASSCP 502
Score = 31.6 bits (70), Expect = 0.87
Identities = 17/44 (38%), Positives = 20/44 (44%), Gaps = 1/44 (2%)
Frame = +2
Query: 227 RGKGRDMRQVQCFTCKQFGHVARSC-TAKFCKYCKQNGHVIFDC 269
RG RD C CK+ GH AR C C C GH+ +C
Sbjct: 320 RGFSRDNL---CKNCKRPGHFARECPNVAICHNCGLPGHIASEC 442
>AV764934
Length = 406
Score = 35.4 bits (80), Expect = 0.061
Identities = 24/81 (29%), Positives = 34/81 (41%), Gaps = 7/81 (8%)
Frame = -2
Query: 460 WHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASISCP-------VCKLAKSKTLPFPSGAH 512
WH +LGHP+ L + + C S S C L K+ LP
Sbjct: 213 WHSRLGHPHLDNLKRVLSS---------CNVSYSPKDTVEFRTACCLGKAHRLPSQMSTT 61
Query: 513 RASNCFEMIHSDVWGMSPIAS 533
FE+I+SD+WG +P+ S
Sbjct: 60 TYLP-FELIYSDLWGPAPVCS 1
>BP066067
Length = 505
Score = 35.4 bits (80), Expect = 0.061
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = -2
Query: 334 WFLDSGASNHMTGSSEYLHNLHSYHGNQQIQIADGNKLSITDVG 377
W DSGAS+H+T ++L + G +Q+ + G L I +G
Sbjct: 297 W*ADSGASHHVTNDPQHLMESITMPGQEQVYVCSGQGLPILSIG 166
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 34.7 bits (78), Expect = 0.10
Identities = 21/72 (29%), Positives = 29/72 (40%), Gaps = 4/72 (5%)
Frame = +2
Query: 228 GKGRDMRQVQCFTCKQFGHVARSCTA----KFCKYCKQNGHVIFDCPIRPPRRTQYPTQA 283
G+G +CF C GH AR C A C C GHV +C P + +
Sbjct: 341 GRGPPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNSPKKNEWQTWKE 520
Query: 284 LHATTSSAAPPT 295
L + T + P+
Sbjct: 521 LFSFTGQVSFPS 556
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.338 0.147 0.468
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,969,045
Number of Sequences: 28460
Number of extensions: 406221
Number of successful extensions: 3989
Number of sequences better than 10.0: 87
Number of HSP's better than 10.0 without gapping: 3875
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3968
length of query: 1343
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1242
effective length of database: 2,023,140
effective search space: 2512739880
effective search space used: 2512739880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144731.14