BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144730.8 - phase: 0
(840 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF177509 69 2e-12
BP030148 60 2e-09
AV766619 49 3e-06
TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-li... 46 3e-05
BP049972 43 2e-04
BP036178 41 7e-04
TC17953 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17... 40 0.002
AV415611 37 0.010
BP083536 37 0.017
TC18180 weakly similar to GB|AAF04891.1|6175165|ATAC011437 Mutat... 35 0.064
AV765466 32 0.32
TC9399 similar to UP|GBF_DICDI (P36417) G-box binding factor (GB... 32 0.42
AV406521 32 0.54
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 32 0.54
TC15394 similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, partial (16%) 30 1.2
TC9034 30 1.2
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 30 2.1
CB827002 29 2.7
TC18056 weakly similar to UP|RCL1_ARATH (Q9C578) Probable RNA 3'... 29 3.5
TC14616 28 4.6
>BF177509
Length = 472
Score = 69.3 bits (168), Expect = 2e-12
Identities = 45/159 (28%), Positives = 69/159 (43%)
Frame = +1
Query: 586 DLGAWEWLMAVEPKKWCKHAFTFYSKCDVLMNNISESFNVTILSARDQPIISMAEWIRHY 645
+ G W+ + P+ W A+ + L NI ES N IL A PII M E IR
Sbjct: 1 EFGTRYWIRRIPPRLWAT-AYFEGQRFGHLTANIVESLNTWILEASGLPIIQMMECIRRQ 177
Query: 646 LMRRMTTSATKLQKWQHNVMPMPRKRLDKEITLAAHWKSTWSGIGEQFQVMHTYNRQQFI 705
LM +W ++P + + + + A ++ + E + H + I
Sbjct: 178 LMTWFNERRETSMQWASILVPSAERCVAEALERARTYQVLRANEAEFEVISHEGSN---I 348
Query: 706 VDIAKRSCSCNFWEIVGIPCRHVVSALGKRKQRPEMFVD 744
VDI R C C W++ G+PC H V+AL +Q F +
Sbjct: 349 VDIRNRCCLCRGWQLYGLPCAHAVAALLSCRQNVHRFTE 465
>BP030148
Length = 398
Score = 59.7 bits (143), Expect = 2e-09
Identities = 39/129 (30%), Positives = 63/129 (48%)
Frame = +3
Query: 514 RYVFISDQQKGLLSVFDEMFDSIDHRLCLRHLYANFKKKFGGGSQIRDLMMGATKATYNQ 573
R +SD+QKG++ + F + H C+RHL +F+K+F + L A T +
Sbjct: 18 RLTILSDRQKGIVDGVEANFPTAFHGFCMRHLSDSFRKEFNNTMLVNLLWEAAHVLTVIE 197
Query: 574 GWLEKMNELKKIDLGAWEWLMAVEPKKWCKHAFTFYSKCDVLMNNISESFNVTILSARDQ 633
+ K+ E+++I A W+ + P+ W A+ + L NI ES + IL A
Sbjct: 198 -FESKILEIEEISQDAAYWIRRIPPRLWAT-AYFEGQRFGHLTANIVESLDTWILDASGL 371
Query: 634 PIISMAEWI 642
PI+ M E I
Sbjct: 372 PIVQMTECI 398
>AV766619
Length = 412
Score = 49.3 bits (116), Expect = 3e-06
Identities = 35/138 (25%), Positives = 60/138 (43%), Gaps = 6/138 (4%)
Frame = +2
Query: 509 IGEDKRYVFISDQQKGLLSVFDEMFDSIDHRLCLRHLYANFKKKFGG--GSQIRDLMMG- 565
I + F+++ Q GL E+F+ H CLR+L K G + R M+
Sbjct: 2 ISTSEPITFVANCQNGLKKSLSEIFEKCHHSYCLRYLAEKLNKDLKGQFSHEARRFMIND 181
Query: 566 ---ATKATYNQGWLEKMNELKKIDLGAWEWLMAVEPKKWCKHAFTFYSKCDVLMNNISES 622
A A + + + +K I A+ W+M EP+ W +AF ++ + L +N +
Sbjct: 182 FYAAAYAPKQETFERSVENMKGISSEAFNWVMQNEPEHWA-NAFFNGARYNHLTSNFGQQ 358
Query: 623 FNVTILSARDQPIISMAE 640
F + A + PI M +
Sbjct: 359 FYNWMSEAHELPITQMID 412
>TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-like
transposase {Arabidopsis thaliana;} , partial (12%)
Length = 588
Score = 45.8 bits (107), Expect = 3e-05
Identities = 29/94 (30%), Positives = 47/94 (49%), Gaps = 10/94 (10%)
Frame = +3
Query: 747 YSRSKYALCYSFAISPINGMDMWPEV--------EAPELL--PPNYKNGPGRPRKLRIRE 796
++ + Y YS I PI +W E+ +A ++L PP PGRPRK R+R
Sbjct: 12 FTVATYRKTYSQTIHPIPDKSLWKELSEGDENAAKADQVLINPPKSLRPPGRPRKKRVRA 191
Query: 797 FDENGSRMRRQGVAYRCTKCDQFGHNQRRCKSDV 830
E+ R++R C++C+Q GH + C + +
Sbjct: 192 --EDRGRVKR---VVHCSRCNQTGHFRTTCAAPI 278
>BP049972
Length = 496
Score = 43.1 bits (100), Expect = 2e-04
Identities = 28/108 (25%), Positives = 45/108 (40%), Gaps = 19/108 (17%)
Frame = -1
Query: 742 FVDDYYSRSKYALCYSFAISPINGMDMWPEVEAPE-------------------LLPPNY 782
F + ++ + Y YS I PI W E+ + + PP
Sbjct: 490 FTESCFTVATYRKTYSQTIHPIPDRSFWNELSIEDANANKAVEDANASQSIEIVINPPKS 311
Query: 783 KNGPGRPRKLRIREFDENGSRMRRQGVAYRCTKCDQFGHNQRRCKSDV 830
PGRPRK R+R E+ R++R C++C+Q GH + C + +
Sbjct: 310 LRPPGRPRKKRVRA--EDRGRVKR---VVHCSRCNQTGHFRTTCAAPI 182
>BP036178
Length = 561
Score = 41.2 bits (95), Expect = 7e-04
Identities = 28/86 (32%), Positives = 39/86 (44%), Gaps = 11/86 (12%)
Frame = -2
Query: 752 YALCYSFAISPINGMDMWPEV-EAPE----------LLPPNYKNGPGRPRKLRIREFDEN 800
Y + YS I PI W E E E + PP + PGRP+K +R EN
Sbjct: 560 YRMAYSQIIIPIPDRSQWREHGEGAEGVGGARVDIVIRPPKTRRPPGRPKKKVLRV--EN 387
Query: 801 GSRMRRQGVAYRCTKCDQFGHNQRRC 826
R +R +C +C GH+Q++C
Sbjct: 386 FKRPKR---IVQCGRCHMLGHSQKKC 318
>TC17953 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;}, partial (6%)
Length = 527
Score = 39.7 bits (91), Expect = 0.002
Identities = 19/57 (33%), Positives = 31/57 (54%)
Frame = +3
Query: 774 APELLPPNYKNGPGRPRKLRIREFDENGSRMRRQGVAYRCTKCDQFGHNQRRCKSDV 830
A + PP K PGRP+ + D ++RQ +C+KC + GHN++ CK+ +
Sbjct: 27 AVTVTPPPTKRPPGRPKMKPVESID----MIKRQ---LQCSKCKELGHNKKTCKNSL 176
>AV415611
Length = 219
Score = 37.4 bits (85), Expect = 0.010
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +3
Query: 705 IVDIAKRSCSCNFWEIVGIPCRHVVSAL 732
+VDI + CSC W++ G+PC H ++ +
Sbjct: 108 VVDIDRWECSCKTWQLTGVPCCHAIAVI 191
>BP083536
Length = 377
Score = 36.6 bits (83), Expect = 0.017
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +1
Query: 711 RSCSCNFWEIVGIPCRHVVSALGKR 735
++C CN WE+V IPCRH ++ + R
Sbjct: 301 KACCCNLWELVEIPCRHALAGVHYR 375
>TC18180 weakly similar to GB|AAF04891.1|6175165|ATAC011437 Mutator-like
transposase {Arabidopsis thaliana;} , partial (4%)
Length = 513
Score = 34.7 bits (78), Expect = 0.064
Identities = 29/93 (31%), Positives = 44/93 (47%), Gaps = 8/93 (8%)
Frame = +3
Query: 743 VDDYYSR----SKYALCYSFAISPINGMDMWPEVEAPE----LLPPNYKNGPGRPRKLRI 794
V DY SR Y L YS ++PI M + PE + + + PP + PGRP R
Sbjct: 6 VYDYCSRYCTTESYRLTYSERVNPIPIMAV-PESKDSQPVVTVTPPFTRRPPGRPATKRY 182
Query: 795 REFDENGSRMRRQGVAYRCTKCDQFGHNQRRCK 827
+ + ++R+ C++C GHN+ CK
Sbjct: 183 ----VSQNIVKRE---LHCSRCKGLGHNKSTCK 260
>AV765466
Length = 599
Score = 32.3 bits (72), Expect = 0.32
Identities = 21/74 (28%), Positives = 36/74 (48%), Gaps = 2/74 (2%)
Frame = -2
Query: 713 CSCNFWEIVGIPCRHVVSALGKR--KQRPEMFVDDYYSRSKYALCYSFAISPINGMDMWP 770
C C +E GI CRH ++ L + K+ P+ +V D + R Y + + + P
Sbjct: 592 CDCRLFEFRGILCRHSLAILSQERVKEVPDRYVLDRW-RKNMRRKYVYVKTSYCVQHLKP 416
Query: 771 EVEAPELLPPNYKN 784
++E ELL +K+
Sbjct: 415 QMERLELLCNQFKS 374
>TC9399 similar to UP|GBF_DICDI (P36417) G-box binding factor (GBF),
partial (3%)
Length = 718
Score = 32.0 bits (71), Expect = 0.42
Identities = 16/49 (32%), Positives = 26/49 (52%)
Frame = +3
Query: 4 FDAVFHHGGEFVMDKRIFYRGGVQTVVSGLESNNWGVCDIQNTVASSGY 52
F A+ HH G+FV + R+ Y V++ E++ W + + VA GY
Sbjct: 510 FKAIIHHSGQFVEEPRLHY-VDVKSNDVHAETDRWSFFECLDVVAEVGY 653
>AV406521
Length = 267
Score = 31.6 bits (70), Expect = 0.54
Identities = 16/45 (35%), Positives = 24/45 (52%), Gaps = 4/45 (8%)
Frame = +1
Query: 485 LAFGVVETECKETWKWFTQLLMEDIGEDKRYV----FISDQQKGL 525
LAFGVV+ E + W WF L + + + F+SD Q+G+
Sbjct: 1 LAFGVVDVENDDNWTWFRSELHKALEASTESMPQMGFLSDGQRGI 135
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 31.6 bits (70), Expect = 0.54
Identities = 18/40 (45%), Positives = 23/40 (57%)
Frame = +3
Query: 121 DEEDVMVSTDDDSGKFVGFDDSEDESTTALEDGFEDVEVE 160
DE+D DDD G+ DD +DE E+G E+VEVE
Sbjct: 291 DEDDDDDDDDDDDGEEEEDDDDDDEE----EEGSEEVEVE 398
>TC15394 similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, partial (16%)
Length = 1093
Score = 30.4 bits (67), Expect = 1.2
Identities = 28/117 (23%), Positives = 47/117 (39%), Gaps = 1/117 (0%)
Frame = +2
Query: 129 TDDDSGKFVGFDDSEDESTTALEDGFEDVEVEAPANGNNRVTVNNKT-LRIKKCGSKTPK 187
+DDD K ++ ED+S + + V+ + + R KT ++ K KTPK
Sbjct: 71 SDDDESKS---EEEEDKSKAQKKTSKKIVKEGSGSKAGERTKSAKKTPVKSSKNVDKTPK 241
Query: 188 KKSPKKNNSGRMNVIAPVSLLKNGKGKYVVDEEIDGDYLSEELGSSDPDDSNDDTIK 244
K +PK+ + + A +S K K E D + D S+ +K
Sbjct: 242 KSTPKQTAAEHDSASASLSKSKQPASKKRKTENEKPDTKGKASSKKQTDKSSKALVK 412
>TC9034
Length = 583
Score = 30.4 bits (67), Expect = 1.2
Identities = 17/52 (32%), Positives = 23/52 (43%), Gaps = 2/52 (3%)
Frame = +1
Query: 780 PNYKNGPGRPRKLRIREFDENG--SRMRRQGVAYRCTKCDQFGHNQRRCKSD 829
P K PG R R R E S R+ +C C++FGH+ C+ D
Sbjct: 43 PTLKRLPGWERINRKRGVTEGPTVSHEARRSHRVKCANCEEFGHDSWECQRD 198
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 29.6 bits (65), Expect = 2.1
Identities = 25/84 (29%), Positives = 34/84 (39%), Gaps = 13/84 (15%)
Frame = +2
Query: 759 AISPINGMDMWPEVEAPELLPPNYKNGPGRPR------KLRIREFDENGSRMRRQGVAY- 811
AI +NGMD+ + P G R R + R R+ D+ G R R +G
Sbjct: 227 AIDAMNGMDLDGRTITVDKAQPQ---GSSRDRDDGDRYRERGRDRDDRGDRDRSRGYGGS 397
Query: 812 ------RCTKCDQFGHNQRRCKSD 829
C KC + GH R C S+
Sbjct: 398 RGSNGGECFKCGKPGHFARECPSE 469
>CB827002
Length = 534
Score = 29.3 bits (64), Expect = 2.7
Identities = 10/19 (52%), Positives = 13/19 (67%)
Frame = +1
Query: 710 KRSCSCNFWEIVGIPCRHV 728
K SCSC +E G+ CRH+
Sbjct: 478 KASCSCQMFEFSGLLCRHI 534
>TC18056 weakly similar to UP|RCL1_ARATH (Q9C578) Probable RNA 3'-terminal
phosphate cyclase-like protein, partial (18%)
Length = 506
Score = 28.9 bits (63), Expect = 3.5
Identities = 13/67 (19%), Positives = 30/67 (44%)
Frame = +2
Query: 475 GRDPNDQYFPLAFGVVETECKETWKWFTQLLMEDIGEDKRYVFISDQQKGLLSVFDEMFD 534
G P + Y+P + + W W + E + + R+ +I+ + +++F ++ D
Sbjct: 101 GLSPFEVYYPTRSQYTISVSQVCWLWHEEPFQEGLIKHGRWCYINPMRAC*IAIFKQLRD 280
Query: 535 SIDHRLC 541
S + C
Sbjct: 281 SAPRKKC 301
>TC14616
Length = 1353
Score = 28.5 bits (62), Expect = 4.6
Identities = 15/64 (23%), Positives = 29/64 (44%), Gaps = 1/64 (1%)
Frame = +2
Query: 744 DDYYSRSKYALCYSFAIS-PINGMDMWPEVEAPELLPPNYKNGPGRPRKLRIREFDENGS 802
+D+ R+ + + +S PI+ M WP P +PP+ + P + R D+
Sbjct: 71 NDHGLRASHRVSHSLRRKEPISSMSDWPAAPTPTTVPPSAPSPQPPPNQNRFLFSDDKSE 250
Query: 803 RMRR 806
++ R
Sbjct: 251 KLTR 262
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.137 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,668,710
Number of Sequences: 28460
Number of extensions: 203792
Number of successful extensions: 1028
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 1009
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1024
length of query: 840
length of database: 4,897,600
effective HSP length: 98
effective length of query: 742
effective length of database: 2,108,520
effective search space: 1564521840
effective search space used: 1564521840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC144730.8