BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144727.5 + phase: 0
(256 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP063694 96 5e-21
BP033602 81 2e-16
AV766088 76 5e-15
BP081717 46 5e-06
BP063902 42 1e-04
BP058111 40 4e-04
BP054351 37 0.003
BP066067 37 0.003
AV764934 35 0.010
BP073434 35 0.017
BP033820 34 0.028
TC12784 similar to UP|Q850H6 (Q850H6) Gag-pol polyprotein (Fragm... 26 0.057
BP054557 33 0.063
TC12255 32 0.082
BP061842 31 0.18
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 31 0.18
BP080584 31 0.24
TC13804 weakly similar to UP|Q8GRK2 (Q8GRK2) Somatic embryogenes... 30 0.53
BP053302 29 0.69
AV767212 29 0.91
>BP063694
Length = 511
Score = 96.3 bits (238), Expect = 5e-21
Identities = 55/152 (36%), Positives = 83/152 (54%), Gaps = 1/152 (0%)
Frame = -2
Query: 97 VVQEQVSGKVIAKGPKVGRLFPLQFISNHLSLPCNNVLN-SYEDWHRKLGHPNSTVLSHL 155
V+Q + +G V+ + L+ L S L + + S+E WH +LGH N ++ L
Sbjct: 489 VIQNRKTGAVLGRXRCDQGLYVLNQDSQALLTTSSPLPRASFELWHSRLGHVNFDIIKQL 310
Query: 156 FKTGLLGNKQVVCTASISCLVCKLAKSKTLPFPSGAHRASNCFEMIHSDVWGMSPIASHA 215
K G L ++ I C C++AKSK L F RAS ++IH D+ G SP+AS
Sbjct: 309 HKHGCLDVSSIL-PKPICCTSCQMAKSKRLVFHDNNKRASAVLDLIHCDLRGPSPVASID 133
Query: 216 RYKYFVTFIDDYSRFTWIYFLRSKSEVFSIVI 247
+ YFV F+DD+SRFTW Y L+ KS+ +++
Sbjct: 132 GFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLL 37
>BP033602
Length = 533
Score = 80.9 bits (198), Expect = 2e-16
Identities = 43/101 (42%), Positives = 59/101 (57%)
Frame = +3
Query: 128 LPCNNVLNSYEDWHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASISCLVCKLAKSKTLPF 187
+ C +V + WH +LGHPN L HLF K V C+ S+ C C LAK++ P+
Sbjct: 243 MSCTSVRDQIMLWHNRLGHPNFQYLRHLFPDLF---KNVNCS-SLECESCVLAKNQRAPY 410
Query: 188 PSGAHRASNCFEMIHSDVWGMSPIASHARYKYFVTFIDDYS 228
S + AS F +IHSDVWG S I + ++FVTFIDD++
Sbjct: 411 YSQPYHASRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>AV766088
Length = 501
Score = 76.3 bits (186), Expect = 5e-15
Identities = 46/145 (31%), Positives = 75/145 (51%)
Frame = -3
Query: 99 QEQVSGKVIAKGPKVGRLFPLQFISNHLSLPCNNVLNSYEDWHRKLGHPNSTVLSHLFKT 158
Q +G + + G L+P+ LS P + S WH +LGHP+S+ L +L
Sbjct: 406 QHHQTGIPLMRCNSPGDLYPVT-----LSFPFAGIAQSL--WHSRLGHPSSSALRYLRSN 248
Query: 159 GLLGNKQVVCTASISCLVCKLAKSKTLPFPSGAHRASNCFEMIHSDVWGMSPIASHARYK 218
+ + + S C C K LPF S + F+++HSD+W SP+ S A ++
Sbjct: 247 KFISYE--LLNYSPVCESCVFGKHVRLPFVSSNNVTVMPFDILHSDLW-TSPVLSSAGHR 77
Query: 219 YFVTFIDDYSRFTWIYFLRSKSEVF 243
++V F+DD++ F W + L +KS+VF
Sbjct: 76 FYVLFLDDFTDFLWTFPLSNKSQVF 2
>BP081717
Length = 291
Score = 46.2 bits (108), Expect = 5e-06
Identities = 23/62 (37%), Positives = 33/62 (53%)
Frame = -1
Query: 137 YEDWHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASISCLVCKLAKSKTLPFPSGAHRASN 196
++ WH +LGHP+S VL H+ K V ++I C C +AK LPFP ++
Sbjct: 174 FDMWHFRLGHPSSKVLQHISKV----FPYVKLHSNIICDFCHMAKQTKLPFPISDTKSKA 7
Query: 197 CF 198
CF
Sbjct: 6 CF 1
>BP063902
Length = 494
Score = 41.6 bits (96), Expect = 1e-04
Identities = 24/55 (43%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Frame = -3
Query: 140 WHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASIS---CLVCKLAKSKTLPFPSGA 191
WH +LGHP S+ L+H L NK + C S S C C L K LPF S A
Sbjct: 180 WHHRLGHPASSALNH-----LRNNKLIFCEPSRSSSVCDSCVLGKHVRLPFSSSA 31
Score = 26.9 bits (58), Expect = 3.4
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = -2
Query: 13 PWFLDSGASNHMTGSSE 29
PW++D+GAS+H S +
Sbjct: 319 PWYMDTGASSHTAASQD 269
>BP058111
Length = 570
Score = 40.0 bits (92), Expect = 4e-04
Identities = 23/65 (35%), Positives = 33/65 (50%), Gaps = 1/65 (1%)
Frame = -1
Query: 140 WHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASIS-CLVCKLAKSKTLPFPSGAHRASNCF 198
WH +LGH +S L+ L K + C S C C +AK K L FP+ +S F
Sbjct: 183 WHLRLGHTSSKKLAILQKNFPF----ITCKKITSPCDTCHMAKQKKLSFPNSVTLSSEIF 16
Query: 199 EMIHS 203
++IH+
Sbjct: 15 DLIHT 1
>BP054351
Length = 431
Score = 37.4 bits (85), Expect = 0.003
Identities = 25/84 (29%), Positives = 39/84 (45%), Gaps = 6/84 (7%)
Frame = -1
Query: 100 EQVSGKVIAKGPKVGRLFPLQFISNHLS------LPCNNVLNSYEDWHRKLGHPNSTVLS 153
+++SGK+IA L+ + + + + C +V + WH +LGHPN L
Sbjct: 245 DRISGKMIASAKMKDGLYYFEDTFRNKAAQGLCGMSCTSVRDQIMLWHNRLGHPNFQYLR 66
Query: 154 HLFKTGLLGNKQVVCTASISCLVC 177
HLF K V C +S+ C C
Sbjct: 65 HLFPDLF---KNVNC-SSLECESC 6
>BP066067
Length = 505
Score = 37.0 bits (84), Expect = 0.003
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = -2
Query: 14 WFLDSGASNHMTGSSEYLHNLASYHGNQQIQIADGNNLSITDVG 57
W DSGAS+H+T ++L + G +Q+ + G L I +G
Sbjct: 297 W*ADSGASHHVTNDPQHLMESITMPGQEQVYVCSGQGLPILSIG 166
>AV764934
Length = 406
Score = 35.4 bits (80), Expect = 0.010
Identities = 24/81 (29%), Positives = 34/81 (41%), Gaps = 7/81 (8%)
Frame = -2
Query: 140 WHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASISC-------LVCKLAKSKTLPFPSGAH 192
WH +LGHP+ L + + C S S C L K+ LP
Sbjct: 213 WHSRLGHPHLDNLKRVLSS---------CNVSYSPKDTVEFRTACCLGKAHRLPSQMSTT 61
Query: 193 RASNCFEMIHSDVWGMSPIAS 213
FE+I+SD+WG +P+ S
Sbjct: 60 TYLP-FELIYSDLWGPAPVCS 1
>BP073434
Length = 490
Score = 34.7 bits (78), Expect = 0.017
Identities = 15/54 (27%), Positives = 26/54 (47%)
Frame = -2
Query: 4 HVKSSNVSRPWFLDSGASNHMTGSSEYLHNLASYHGNQQIQIADGNNLSITDVG 57
H ++ + W+ DSGAS+H+T + + G I + +G LS+ G
Sbjct: 486 HTPAAGLVASWYPDSGASHHVTPHGNNIQYPDPFEGPDHIFVGNGQGLSVASSG 325
Score = 29.3 bits (64), Expect = 0.69
Identities = 17/59 (28%), Positives = 24/59 (39%), Gaps = 7/59 (11%)
Frame = -1
Query: 135 NSYEDWHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASIS-------CLVCKLAKSKTLP 186
N + WH +LGHP+ L + ++ C +S C C L KS LP
Sbjct: 187 NKFLIWHSRLGHPHQDALQAVLQS---------CNIHLSSRDMTDFCYACCLGKSHILP 38
>BP033820
Length = 537
Score = 33.9 bits (76), Expect = 0.028
Identities = 15/31 (48%), Positives = 19/31 (60%), Gaps = 2/31 (6%)
Frame = -2
Query: 127 SLPCNNVLNS--YEDWHRKLGHPNSTVLSHL 155
S PCN+ N YE WH ++GHP+ VL L
Sbjct: 143 SAPCNSSSNISIYELWHNRMGHPHHDVLKQL 51
>TC12784 similar to UP|Q850H6 (Q850H6) Gag-pol polyprotein (Fragment),
partial (15%)
Length = 460
Score = 26.2 bits (56), Expect(2) = 0.057
Identities = 10/16 (62%), Positives = 12/16 (74%)
Frame = +1
Query: 220 FVTFIDDYSRFTWIYF 235
FVTF+DDYS T + F
Sbjct: 385 FVTFVDDYSSCTMVIF 432
Score = 25.8 bits (55), Expect = 7.7
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +1
Query: 17 DSGASNHMTGSS 28
DSGA+NHMTG S
Sbjct: 1 DSGATNHMTGDS 36
Score = 25.4 bits (54), Expect(2) = 0.057
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = +3
Query: 198 FEMIHSDVWGMSPIASHARYKY 219
F + SDVWG P+ S Y+Y
Sbjct: 318 FILSTSDVWGPCPVTSKLGYEY 383
>BP054557
Length = 538
Score = 32.7 bits (73), Expect = 0.063
Identities = 42/163 (25%), Positives = 66/163 (39%), Gaps = 21/163 (12%)
Frame = -2
Query: 16 LDSGASNHMTGSSEYLHNLASYHG------NQQIQIADGNNLSITDVGDINSDF--RNVL 67
LD+GA++H+ + + L H N Q +IA + D + F VL
Sbjct: 534 LDTGATHHVCHNLDVFQALRQVHPVLLSMPNGQKEIAH-----MADSVYFSEKFFLAXVL 370
Query: 68 VSPGLASNLLSVGQLVDN-NCNVNFSRAGCVVQEQVSGKVIAKGPKVGRLFPLQFISNHL 126
P NL+SV LV + NC + C++QE+ + K I + G L+ + +
Sbjct: 369 YVPSFNFNLISVSALVKSLNCRLVIFDECCLLQERPTLKTIGAAEERGGLYAVIDPAVKA 190
Query: 127 SLPC--NNVLNSYED----------WHRKLGHPNSTVLSHLFK 157
P N+ WH +LGH +S L+ L K
Sbjct: 189 KHPPAEGNIKTQVSHFNFCQFDCNLWHLRLGHTSSKKLAILQK 61
>TC12255
Length = 558
Score = 32.3 bits (72), Expect = 0.082
Identities = 14/28 (50%), Positives = 18/28 (64%), Gaps = 2/28 (7%)
Frame = +1
Query: 127 SLPCNNVLNS--YEDWHRKLGHPNSTVL 152
S PCN+ N YE WH ++GHP+ VL
Sbjct: 412 SAPCNSSSNISIYELWHNRMGHPHHDVL 495
Score = 27.3 bits (59), Expect = 2.6
Identities = 12/46 (26%), Positives = 22/46 (47%)
Frame = +3
Query: 14 WFLDSGASNHMTGSSEYLHNLASYHGNQQIQIADGNNLSITDVGDI 59
W DSGA++H+T + + Q+ + +G L I +G +
Sbjct: 51 WCPDSGATHHVTNNPGIFVDNVPLATQDQLLVGNGQGLPIQSIGHL 188
>BP061842
Length = 318
Score = 31.2 bits (69), Expect = 0.18
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = -3
Query: 199 EMIHSDVWGMSPIASHARYKYFVTFID 225
E++ D+WG +P S +KY+++F+D
Sbjct: 85 ELVPCDLWGPAPCVSTNGFKYYLSFVD 5
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 31.2 bits (69), Expect = 0.18
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = +3
Query: 174 CLVCKLAKSKTLPFPSGAHRASNCFEMIHSDVWGMSPIAS 213
C C L K LPF S F+++HSD+W SP+ S
Sbjct: 1029 CDSCVLGKHVRLPFSSSETITLRPFDILHSDLW-TSPVLS 1145
>BP080584
Length = 507
Score = 30.8 bits (68), Expect = 0.24
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 3/58 (5%)
Frame = -3
Query: 127 SLPCNNVLNSYEDWHRKLGHPN---STVLSHLFKTGLLGNKQVVCTASISCLVCKLAK 181
+L CN + + + WH +LGHP+ + +F +VC C +C LAK
Sbjct: 154 NLACNFIPSMHNLWHFRLGHPSFVKEQSIRDIF-------PYIVCNKEQVCDICPLAK 2
>TC13804 weakly similar to UP|Q8GRK2 (Q8GRK2) Somatic embryogenesis receptor
kinase 1, partial (7%)
Length = 602
Score = 29.6 bits (65), Expect = 0.53
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -1
Query: 181 KSKTLPFPSGAHRASNCFEMIHSDVWGMS 209
KSK +PF A++A C ++H D G S
Sbjct: 365 KSKGIPFQQKAYQAGMCINLVHRDYVGYS 279
>BP053302
Length = 432
Score = 29.3 bits (64), Expect = 0.69
Identities = 22/67 (32%), Positives = 32/67 (46%), Gaps = 13/67 (19%)
Frame = -1
Query: 103 SGKVIAKGPKVGRLFPLQFISNHLSLPCNN---------VLNSYED----WHRKLGHPNS 149
SGK+I + + G L+ L + + LP VLN+ +D WH +LGHP+
Sbjct: 234 SGKMIGRAKESGGLYYLD-LGSASPLPPKTISSCFESFFVLNNNDDHVMLWHLRLGHPSF 58
Query: 150 TVLSHLF 156
L LF
Sbjct: 57 HYLKPLF 37
>AV767212
Length = 330
Score = 28.9 bits (63), Expect = 0.91
Identities = 11/21 (52%), Positives = 16/21 (75%)
Frame = -2
Query: 126 LSLPCNNVLNSYEDWHRKLGH 146
LS C+N+++SY HR+LGH
Sbjct: 242 LSSECDNLISSYLPQHRRLGH 180
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,985,597
Number of Sequences: 28460
Number of extensions: 73646
Number of successful extensions: 402
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 392
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 395
length of query: 256
length of database: 4,897,600
effective HSP length: 88
effective length of query: 168
effective length of database: 2,393,120
effective search space: 402044160
effective search space used: 402044160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144727.5