BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144725.4 + phase: 0
(126 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8297 similar to UP|Q40828 (Q40828) WRKY3, partial (27%) 60 8e-11
TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-bi... 60 8e-11
TC7955 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcrip... 58 4e-10
BP046076 57 7e-10
AV408330 56 2e-09
AV420160 55 4e-09
TC15775 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcri... 55 5e-09
TC11124 similar to UP|Q8H9D7 (Q8H9D7) WRKY-type DNA binding prot... 54 6e-09
TC14909 similar to UP|WR21_ARATH (O04336) Probable WRKY transcri... 54 8e-09
AV423663 54 8e-09
TC11734 similar to UP|WR49_ARATH (Q9FHR7) Probable WRKY transcri... 54 1e-08
TC9067 similar to UP|WR15_ARATH (O22176) Probable WRKY transcrip... 54 1e-08
TC9074 similar to UP|WR53_ARATH (Q9SUP6) Probable WRKY transcrip... 53 2e-08
TC16534 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcri... 48 6e-07
TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription fact... 47 7e-07
TC14849 similar to UP|Q40090 (Q40090) SPF1 protein, partial (37%) 46 2e-06
TC11031 homologue to UP|WR65_ARATH (Q9LP56) Probable WRKY transc... 46 2e-06
AV427157 44 8e-06
TC8298 homologue to UP|Q9SXP4 (Q9SXP4) DNA-binding protein NtWRK... 35 0.004
TC18024 weakly similar to PIR|F86410|F86410 protein F3M18.12 [im... 31 0.072
>TC8297 similar to UP|Q40828 (Q40828) WRKY3, partial (27%)
Length = 617
Score = 60.5 bits (145), Expect = 8e-11
Identities = 28/59 (47%), Positives = 34/59 (57%)
Frame = +1
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P D F W KYG K I G YPR YY+C+ K C A+K V++ D PN+ V Y G H
Sbjct: 166 PTDDFQWRKYGQKPIKGSPYPRGYYKCSSEK--GCPARKHVERAQDDPNMLIVTYEGEH 336
>TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-binding
protein {Arabidopsis thaliana;} , partial (45%)
Length = 1257
Score = 60.5 bits (145), Expect = 8e-11
Identities = 27/59 (45%), Positives = 36/59 (60%)
Frame = +3
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P D ++W KYG K I G YPR YY+C+ V+ C A+K V++ D PN+ V Y G H
Sbjct: 648 PPDEYSWRKYGQKPIKGSPYPRGYYKCSTVR--GCPARKHVERAQDDPNMLIVTYEGEH 818
>TC7955 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcription
factor 11 (WRKY DNA-binding protein 11), partial (27%)
Length = 579
Score = 58.2 bits (139), Expect = 4e-10
Identities = 26/59 (44%), Positives = 35/59 (59%)
Frame = +3
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P D ++W KYG K I G YPR YY+C+ V+ C A+K V++ D P + V Y G H
Sbjct: 87 PPDEYSWRKYGQKPIKGSPYPRGYYKCSTVR--GCPARKHVERATDDPTMLIVTYEGEH 257
>BP046076
Length = 593
Score = 57.4 bits (137), Expect = 7e-10
Identities = 24/58 (41%), Positives = 34/58 (58%)
Frame = -2
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
+DG+ W KYG K + ++PRSYYRC H CR KK+V++L + + Y G H
Sbjct: 457 DDGYKWRKYGQKVVKNSLHPRSYYRCTHSN---CRVKKRVERLSEDCRMVITTYEGRH 293
>AV408330
Length = 316
Score = 56.2 bits (134), Expect = 2e-09
Identities = 25/59 (42%), Positives = 33/59 (55%)
Frame = +3
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
EDG+ W KYG K + +PRSYYRC +C KK+V++ P+I Y G HT
Sbjct: 129 EDGYRWRKYGQKAVKNSPFPRSYYRCTSA---SCNVKKRVERSFTDPSIVVTTYEGQHT 296
>AV420160
Length = 417
Score = 55.1 bits (131), Expect = 4e-09
Identities = 36/93 (38%), Positives = 44/93 (46%), Gaps = 1/93 (1%)
Frame = +1
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
PP D + W KYG K I G YPR YYRC+ K C A+K+V++ P V Y+ H
Sbjct: 148 PPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK--GCPARKQVERSRVDPTKLMVTYSYEH 321
Query: 62 TCRMSLTIP-SLFVPARQPLDISIDGIQSTMPT 93
SL +P S A S DG T T
Sbjct: 322 --NHSLPVPKSHSSAASATATTSSDGAADTPAT 414
>TC15775 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcription
factor 17 (WRKY DNA-binding protein 17), partial (53%)
Length = 1284
Score = 54.7 bits (130), Expect = 5e-09
Identities = 25/59 (42%), Positives = 34/59 (57%)
Frame = +3
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P D ++W KYG K I G YPR YY+C+ V+ C A+K V++ D P + V Y H
Sbjct: 735 PADEYSWRKYGQKPIKGTPYPRGYYKCSTVR--GCPARKHVERATDDPAMLIVTYEDEH 905
>TC11124 similar to UP|Q8H9D7 (Q8H9D7) WRKY-type DNA binding protein,
partial (45%)
Length = 517
Score = 54.3 bits (129), Expect = 6e-09
Identities = 24/58 (41%), Positives = 30/58 (51%)
Frame = +1
Query: 5 DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
DG+ W KYG K + +PRSYYRC H + C KK V +L + Y G HT
Sbjct: 1 DGYRWRKYGQKAVKNNKFPRSYYRCTH---HGCHVKKPVPRLTQDEGVVVTTYEGVHT 165
>TC14909 similar to UP|WR21_ARATH (O04336) Probable WRKY transcription
factor 21 (WRKY DNA-binding protein 21), partial (22%)
Length = 590
Score = 53.9 bits (128), Expect = 8e-09
Identities = 23/59 (38%), Positives = 35/59 (58%)
Frame = +3
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P D ++W KYG K I G +PR YY+C+ ++ C A+K V++ + P + V Y G H
Sbjct: 57 PPDDYSWRKYGQKPIKGSPHPRGYYKCSSMR--GCPARKHVERCLEEPTMLLVTYEGEH 227
>AV423663
Length = 447
Score = 53.9 bits (128), Expect = 8e-09
Identities = 26/57 (45%), Positives = 33/57 (57%)
Frame = +1
Query: 5 DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
D + W KYG K I G YPR YYRC+ K C A+K+V++ P +F V Y G H
Sbjct: 82 DIWAWRKYGQKPIKGSPYPRGYYRCSSSK--GCLARKQVERNKSEPAMFIVTYTGEH 246
>TC11734 similar to UP|WR49_ARATH (Q9FHR7) Probable WRKY transcription
factor 49 (WRKY DNA-binding protein 49), partial (28%)
Length = 1009
Score = 53.5 bits (127), Expect = 1e-08
Identities = 24/58 (41%), Positives = 33/58 (56%)
Frame = +3
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
+DG+ W KYG K I PRSYYRC + + C AKK+V++ + P + Y G H
Sbjct: 333 DDGYKWRKYGQKSIKNSPNPRSYYRCTNPR---CSAKKQVERSNEDPETLIITYEGLH 497
>TC9067 similar to UP|WR15_ARATH (O22176) Probable WRKY transcription
factor 15 (WRKY DNA-binding protein 15), partial (31%)
Length = 660
Score = 53.5 bits (127), Expect = 1e-08
Identities = 25/64 (39%), Positives = 36/64 (56%)
Frame = +3
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
P D ++W KYG K I G +PR YY+C+ V+ C A+K V++ D + V Y G H
Sbjct: 120 PPDDYSWRKYGQKPIKGSPHPRGYYKCSSVR--GCPARKHVERALDDAAMLVVTYEGEHN 293
Query: 63 CRMS 66
+S
Sbjct: 294 HALS 305
>TC9074 similar to UP|WR53_ARATH (Q9SUP6) Probable WRKY transcription
factor 53 (WRKY DNA-binding protein 53), partial (15%)
Length = 1044
Score = 52.8 bits (125), Expect = 2e-08
Identities = 20/45 (44%), Positives = 28/45 (61%)
Frame = +3
Query: 19 GFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
G +PR YYRC H + C+A K+VQ+ P ++E+ Y G HTC
Sbjct: 6 GAKFPRGYYRCTHRNVQGCQATKQVQKSDKDPTMYEINYRGKHTC 140
>TC16534 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcription
factor 41 (WRKY DNA-binding protein 41), partial (20%)
Length = 697
Score = 47.8 bits (112), Expect = 6e-07
Identities = 20/38 (52%), Positives = 23/38 (59%)
Frame = +1
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRA 39
P +DG+ W KYG K+I G YPRSYYRC C A
Sbjct: 580 PHDDGYNWRKYGQKDILGAKYPRSYYRCTFRNTQNCWA 693
>TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription factor 1,
partial (41%)
Length = 1717
Score = 47.4 bits (111), Expect = 7e-07
Identities = 33/115 (28%), Positives = 53/115 (45%)
Frame = +1
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
+DG+ W KYG K PR+Y++C+ +C KKKVQ+ D ++ Y G H
Sbjct: 583 KDGYQWRKYGQKVTRDNPCPRAYFKCSFAP--SCPVKKKVQRSVDDQSVLVATYEGEHNH 756
Query: 64 RMSLTIPSLFVPARQPLDISIDGIQSTMPTSSTLYSRRISSGMPGIFLLPINNER 118
+ + R ++S+ G +MP+S +L SS P + L + R
Sbjct: 757 PQPSQMEATSGSGR---NVSLVG---SMPSSKSL-----SSPAPAVVTLDLTKSR 888
>TC14849 similar to UP|Q40090 (Q40090) SPF1 protein, partial (37%)
Length = 1389
Score = 45.8 bits (107), Expect = 2e-06
Identities = 21/58 (36%), Positives = 28/58 (48%)
Frame = +2
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
+DG+ W KYG K + G PRSYY+C H C +K V++ Y G H
Sbjct: 404 DDGYRWRKYGQKVVKGNPNPRSYYKCTHP---GCPVRKHVERASHDLRAVITTYEGKH 568
>TC11031 homologue to UP|WR65_ARATH (Q9LP56) Probable WRKY transcription
factor 65 (WRKY DNA-binding protein 65), partial (15%)
Length = 453
Score = 45.8 bits (107), Expect = 2e-06
Identities = 18/32 (56%), Positives = 20/32 (62%)
Frame = +3
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVK 33
PP D + W KYG K I G YPR YYRC+ K
Sbjct: 342 PPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK 437
>AV427157
Length = 379
Score = 43.9 bits (102), Expect = 8e-06
Identities = 20/58 (34%), Positives = 28/58 (47%)
Frame = +3
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
+DG+ W KYG K + G PRSYY+C + C +K V++ Y G H
Sbjct: 207 DDGYRWRKYGQKVVKGNPNPRSYYKCTNA---GCMVRKHVERASHDLKSVITTYEGKH 371
>TC8298 homologue to UP|Q9SXP4 (Q9SXP4) DNA-binding protein NtWRKY3,
partial (16%)
Length = 514
Score = 35.0 bits (79), Expect = 0.004
Identities = 16/38 (42%), Positives = 22/38 (57%)
Frame = +3
Query: 24 RSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
R YY+C+ K C A+K V++ D PN+ V Y G H
Sbjct: 159 RGYYKCSSEK--GCPARKHVERAQDDPNMLIVTYEGEH 266
>TC18024 weakly similar to PIR|F86410|F86410 protein F3M18.12 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(4%)
Length = 485
Score = 30.8 bits (68), Expect = 0.072
Identities = 22/59 (37%), Positives = 30/59 (50%), Gaps = 2/59 (3%)
Frame = -3
Query: 38 RAKKKVQQLGDHPNIFEVRYNGGHTCRMSLTIPSLFVPAR--QPLDISIDGIQSTMPTS 94
RA ++V++ GD + R G TC IPS F R QPL + + I T+PTS
Sbjct: 204 RAVQRVEKHGD*--VARQRCTGQRTCGTGAEIPSTFGQFRQLQPLSLVSNRITGTIPTS 34
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.139 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,505,758
Number of Sequences: 28460
Number of extensions: 34355
Number of successful extensions: 179
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of query: 126
length of database: 4,897,600
effective HSP length: 80
effective length of query: 46
effective length of database: 2,620,800
effective search space: 120556800
effective search space used: 120556800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)
Medicago: description of AC144725.4