BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144724.6 + phase: 0
(1523 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 199 1e-55
BP084332 164 1e-40
AV780009 124 1e-28
BP043850 83 4e-16
BP057233 81 1e-15
BP075943 75 1e-13
BP052632 69 4e-12
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 67 2e-11
BP063694 64 1e-10
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 62 9e-10
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 61 2e-09
AV424544 61 2e-09
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 60 2e-09
AV766088 56 5e-08
BP054819 54 2e-07
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 52 5e-07
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 50 2e-06
AV766665 50 4e-06
BP033602 47 3e-05
AU088980 45 7e-05
>BP066094
Length = 532
Score = 199 bits (507), Expect(3) = 1e-55
Identities = 92/149 (61%), Positives = 125/149 (83%)
Frame = +2
Query: 1099 ARLEAIRILLAYAAHKSIKLFQMDVKSAFLNGFLNEEVYVSQPPGFINKEKPNHVFKLTK 1158
++ EAIR+L++++ + +I L QM+VKSAFLNG+++EEVYV QPPG +++ +H+FKL K
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 1159 ALYGLKQAPRAWYDRLSTFLIENGFSRGKIDTTLFRKTHNTDLLIVQVYVDDIIFGATKI 1218
+LYGLKQAPRAWY+RLS+FL+EN RGK+DTTLF KT+ D+LIVQ+YVDDIIFG+
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 1219 KMCEEFSNLMQSEFEMSMMGELGFFLGLQ 1247
+C+EFS +MQ+EFEM MMGEL +FLG+Q
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 33.9 bits (76), Expect(3) = 1e-55
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = +3
Query: 1083 YNQQEGIDYDETFAPVAR 1100
Y+QQ GIDY ETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 1e-55
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 1073 RNKARLVAQG 1082
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 164 bits (414), Expect = 1e-40
Identities = 75/112 (66%), Positives = 97/112 (85%)
Frame = +2
Query: 1403 QNTIALSTTEAEYVSAASCCSQILWVRNQLEDYSLRYTSVPIYCDNTSAINLSKNPIQHS 1462
Q+TIALST EAEY+SAA C +Q+LW+++QLEDY + +++PIYCDNT+AI+LSKNPI HS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 1463 RSKHIEIKHHFIRDHVQKKNIALSFVDTENQLADIFTKPLVEDRFNFLKEKL 1514
R+KHIE+K+HFIRD+VQK + L FVDT++Q ADIFTKPL EDRFNF+ + L
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 367
>AV780009
Length = 529
Score = 124 bits (311), Expect = 1e-28
Identities = 65/163 (39%), Positives = 97/163 (58%), Gaps = 1/163 (0%)
Frame = -1
Query: 1340 AVKRIFRYLVGTTDLGLWYRKGSSFDLVAYCDADYAGDKVERKSTSGSCQFLGQALIGWS 1399
A R+ RY+ G GL++ S L AY D+D+AG R+S +G FLG +LI W
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 1400 CRKQNTIALSTTEAEYVSAASCCSQILWVRNQLEDYSLRY-TSVPIYCDNTSAINLSKNP 1458
+KQ T++ S++EAEY + A+ ++ W+ + L VP++CDN SA++++ NP
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNP 167
Query: 1459 IQHSRSKHIEIKHHFIRDHVQKKNIALSFVDTENQLADIFTKP 1501
H R+KHIE+ H +R +Q I L + T +QLADIFTKP
Sbjct: 166 TFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>BP043850
Length = 515
Score = 82.8 bits (203), Expect = 4e-16
Identities = 39/99 (39%), Positives = 62/99 (62%), Gaps = 1/99 (1%)
Frame = -1
Query: 1423 SQILWVRNQLEDYS-LRYTSVPIYCDNTSAINLSKNPIQHSRSKHIEIKHHFIRDHVQKK 1481
S+I+W+R L + L+ P++ DNTSAI ++ NP+ H ++HIE+ H +R+ ++
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 1482 NIALSFVDTENQLADIFTKPLVEDRFNFLKEKLLIMKNP 1520
I L V T Q+ADI TK L R NFL KL+++++P
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKLMLVESP 219
>BP057233
Length = 473
Score = 80.9 bits (198), Expect = 1e-15
Identities = 36/77 (46%), Positives = 55/77 (70%)
Frame = -2
Query: 1444 IYCDNTSAINLSKNPIQHSRSKHIEIKHHFIRDHVQKKNIALSFVDTENQLADIFTKPLV 1503
++CDN SA L+ NP+ H+RSKHIEI H+IRD V + + +++V T +Q+AD TKPL
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1504 EDRFNFLKEKLLIMKNP 1520
RF+ L++KL ++ +P
Sbjct: 265 HTRFSQLRDKLGVIHSP 215
>BP075943
Length = 547
Score = 74.7 bits (182), Expect = 1e-13
Identities = 44/163 (26%), Positives = 77/163 (46%), Gaps = 5/163 (3%)
Frame = -3
Query: 1206 VYVDDIIFGATKIKMCEEFSNLMQSEFEMSMMGELGFFLGLQIKQHSNGIFISQEKYVKD 1265
+YVDD++ + + + + +F + +GE +FLGL+I + ++GI ++Q KY
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 1266 ILKKYKMNEAKIMSTPMHPSSSLDKDESGKSISEK-----EYRGMIGSLLYLTASRPDIV 1320
++ + P S + G + YR ++G LLYL +RPDI
Sbjct: 335 LIS----DSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDIT 168
Query: 1321 FAVGLCARFQTCAKESHLTAVKRIFRYLVGTTDLGLWYRKGSS 1363
FAV ++F + + H + +YL G+ GL+Y SS
Sbjct: 167 FAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASSS 39
>BP052632
Length = 489
Score = 69.3 bits (168), Expect = 4e-12
Identities = 38/92 (41%), Positives = 53/92 (57%), Gaps = 10/92 (10%)
Frame = -3
Query: 871 FGCYCYILNNKEKLGKFDPKSDKAIFLGYSTTSKGYRVYNLKTQTVEISMHIIFD----- 925
FGC +ILN + +GKFD K DK I LGYS+ SK Y V+N +TQ VE S+++ FD
Sbjct: 430 FGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNSRTQVVEESINVKFDD*SIE 251
Query: 926 -----EYDEHSKPKENEDTEVPTLQNVPVQNT 952
E D + ++D EVPT + P + +
Sbjct: 250 ATYRLEKDFLNLDLSDQDEEVPTNEGQPSETS 155
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 67.4 bits (163), Expect = 2e-11
Identities = 34/89 (38%), Positives = 52/89 (58%), Gaps = 1/89 (1%)
Frame = +1
Query: 1427 WVRNQLEDYSLRYTSVP-IYCDNTSAINLSKNPIQHSRSKHIEIKHHFIRDHVQKKNIAL 1485
W+ L+D + + +YCDN SA +++ NP+ H R+KHIEI H +R+ +QK I L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 1486 SFVDTENQLADIFTKPLVEDRFNFLKEKL 1514
+ + LADI+TK L F+ + KL
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKL 270
>BP063694
Length = 511
Score = 64.3 bits (155), Expect = 1e-10
Identities = 50/152 (32%), Positives = 71/152 (45%), Gaps = 8/152 (5%)
Frame = -2
Query: 697 KNLYVLELNDMPAESCFMSLEKDKW-IWHKRAGHISMKTIAKLSQ---LDLVRGLPKISF 752
+ LYVL + + L + + +WH R GH++ I +L + LD+ LPK
Sbjct: 438 QGLYVLNQDSQALLTTSSPLPRASFELWHSRLGHVNFDIIKQLHKHGCLDVSSILPK--- 268
Query: 753 EKDRICEACVKGKQVKSSFKTIEFISTQKP----LELLHIDLFGPVQTASLTGKRYGFVI 808
IC C + KS K + F K L+L+H DL GP AS+ G Y +
Sbjct: 267 ---PIC--CTSCQMAKS--KRLVFHDNNKRASAVLDLIHCDLRGPSPVASIDGFSYFVIF 109
Query: 809 VDDFSRFTWVLFLKHKDESFEAFQNFCKRVQN 840
VDDFSRFTW LK K + + F ++N
Sbjct: 108 VDDFSRFTWFYPLKRKSDFSDVLLRFKVFMEN 13
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 61.6 bits (148), Expect = 9e-10
Identities = 32/92 (34%), Positives = 54/92 (57%), Gaps = 1/92 (1%)
Frame = +1
Query: 1427 WVRNQLEDYSLRYTSVPI-YCDNTSAINLSKNPIQHSRSKHIEIKHHFIRDHVQKKNIAL 1485
W+ L+D + + S + YCD+ SA +++ N + H R+KH++I H +R+ +Q K L
Sbjct: 13 WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHL 192
Query: 1486 SFVDTENQLADIFTKPLVEDRFNFLKEKLLIM 1517
+ + +Q ADI TKPL F+ L KL ++
Sbjct: 193 LPISSVDQTADILTKPLESGPFSHLVSKLGVL 288
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 60.8 bits (146), Expect = 2e-09
Identities = 40/149 (26%), Positives = 75/149 (49%), Gaps = 13/149 (8%)
Frame = +2
Query: 1176 TFLIENGFSRGKID-TTLFRKTHNTDLLIVQVYVDDIIFGATKIKMCEEFSNLMQSEFEM 1234
+F++ G++R D T ++ + D +I+ +YVDD++ +E + EF+M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 1235 SMMGELGFFLGLQI--KQHSNGIFISQEKYVKDILKKYKMNEAKIMSTP----------M 1282
+G LG+QI + I++SQ+ Y++ +L+++ M + +STP M
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 1283 HPSSSLDKDESGKSISEKEYRGMIGSLLY 1311
PSS ++ E +S Y +GSL+Y
Sbjct: 368 IPSSEAERME----MSRVPYASAVGSLMY 442
>AV424544
Length = 276
Score = 60.8 bits (146), Expect = 2e-09
Identities = 29/90 (32%), Positives = 51/90 (56%)
Frame = +3
Query: 1173 RLSTFLIENGFSRGKIDTTLFRKTHNTDLLIVQVYVDDIIFGATKIKMCEEFSNLMQSEF 1232
+LS++L G+ + D +LF K + ++ VYVDD+I + + N + +F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 1233 EMSMMGELGFFLGLQIKQHSNGIFISQEKY 1262
+ +G L +FLGL++ + S G+F+SQ KY
Sbjct: 186 RIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 60.5 bits (145), Expect = 2e-09
Identities = 37/100 (37%), Positives = 53/100 (53%), Gaps = 1/100 (1%)
Frame = -1
Query: 1259 QEKYVKDILKKYKMNEAKIMSTPMHPSS-SLDKDESGKSISEKEYRGMIGSLLYLTASRP 1317
Q+ YV DILKK+KM +K +ST + + GK + Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1318 DIVFAVGLCARFQTCAKESHLTAVKRIFRYLVGTTDLGLW 1357
DIV VGL +RF + H +R RY+ GT G++
Sbjct: 559 DIVCGVGLRSRFME-P*DCH*QGAQRSLRYIKGTLKDGIF 443
>AV766088
Length = 501
Score = 55.8 bits (133), Expect = 5e-08
Identities = 38/111 (34%), Positives = 58/111 (52%), Gaps = 4/111 (3%)
Frame = -3
Query: 722 IWHKRAGHISMKTIAKLSQLDLVRGLPKISFEK---DRICEACVKGKQVKSSFKTIEFIS 778
+WH R GH S S L +R IS+E +CE+CV GK V+ F + +
Sbjct: 307 LWHSRLGHPSS------SALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNV- 149
Query: 779 TQKPLELLHIDLF-GPVQTASLTGKRYGFVIVDDFSRFTWVLFLKHKDESF 828
T P ++LH DL+ PV +++ G R+ + +DDF+ F W L +K + F
Sbjct: 148 TVMPFDILHSDLWTSPVLSSA--GHRFYVLFLDDFTDFLWTFPLSNKSQVF 2
>BP054819
Length = 537
Score = 53.5 bits (127), Expect = 2e-07
Identities = 32/79 (40%), Positives = 40/79 (50%), Gaps = 7/79 (8%)
Frame = -3
Query: 9 EGGSSNRPPLFDGSNYYFWKGKMELFLRSQDNDMWAVITNGDFVPTNKE-------GAVK 61
EGG NRPP+ DG+NY + K +M FL+S DN W I G PT E K
Sbjct: 301 EGG*VNRPPILDGTNYDY*KVRMTAFLKSIDNKTWKAIVKGWKHPTKVEVEGTSTTVVEK 122
Query: 62 AKSAWSTDEKAQVLLNSKA 80
+ W++ E L NSKA
Sbjct: 121 PEEEWTSVEDEAALGNSKA 65
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 52.4 bits (124), Expect = 5e-07
Identities = 32/82 (39%), Positives = 48/82 (58%), Gaps = 3/82 (3%)
Frame = +3
Query: 1424 QILWVRNQLEDYSLRYTS---VPIYCDNTSAINLSKNPIQHSRSKHIEIKHHFIRDHVQK 1480
+ LW+ L D LR S V IYCDN SA++L+ N + H R+++IEI H + V
Sbjct: 21 EALWLTYALAD--LRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLF 194
Query: 1481 KNIALSFVDTENQLADIFTKPL 1502
+ L V + +Q+AD+FTK +
Sbjct: 195 GILHLLHVPSSDQVADVFTKTI 260
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 50.4 bits (119), Expect = 2e-06
Identities = 34/120 (28%), Positives = 60/120 (49%), Gaps = 3/120 (2%)
Frame = +3
Query: 1390 FLGQALIGWSCRKQNTIALSTTEAEYVSAASCCSQILWVRNQLEDYSLRYTSVPIYC--- 1446
F G A + W R Q +ALST EAE+++A C ++LW++N L+ + + S PI C
Sbjct: 12 FAGGA-VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQ--NAWFHSHPILCCIV 182
Query: 1447 DNTSAINLSKNPIQHSRSKHIEIKHHFIRDHVQKKNIALSFVDTENQLADIFTKPLVEDR 1506
+ L++ + + +RD + K + L + T++ AD+ TK L ++
Sbjct: 183 ITKALFTLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREK 362
>AV766665
Length = 601
Score = 49.7 bits (117), Expect = 4e-06
Identities = 29/76 (38%), Positives = 39/76 (51%)
Frame = +3
Query: 1339 TAVKRIFRYLVGTTDLGLWYRKGSSFDLVAYCDADYAGDKVERKSTSGSCQFLGQALIGW 1398
T RIF YL + G ++K + + ADY G V+R ST G FL L+ W
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 1399 SCRKQNTIALSTTEAE 1414
++QN IA S+ EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>BP033602
Length = 533
Score = 46.6 bits (109), Expect = 3e-05
Identities = 35/130 (26%), Positives = 60/130 (45%), Gaps = 6/130 (4%)
Frame = +3
Query: 688 KLFFSGSRRKNLYVLE---LNDMPAESCFMSLEKDK---WIWHKRAGHISMKTIAKLSQL 741
K+ S + LY E N C MS + +WH R GH + + + L
Sbjct: 156 KMIGSAKMKDGLYYFEDTFRNKAAQGLCGMSCTSVRDQIMLWHNRLGHPNFQYLRHLFP- 332
Query: 742 DLVRGLPKISFEKDRICEACVKGKQVKSSFKTIEFISTQKPLELLHIDLFGPVQTASLTG 801
DL + + S E CE+CV K ++ + + + ++ +P L+H D++GP + + G
Sbjct: 333 DLFKNVNCSSLE----CESCVLAKNQRAPYYSQPYHAS-RPFYLIHSDVWGPSKITTQFG 497
Query: 802 KRYGFVIVDD 811
KR+ +DD
Sbjct: 498 KRWFVTFIDD 527
>AU088980
Length = 360
Score = 45.4 bits (106), Expect = 7e-05
Identities = 22/61 (36%), Positives = 30/61 (49%)
Frame = +2
Query: 856 EFENASFKTFFDENVFGCYCYILNNKEKLGKFDPKSDKAIFLGYSTTSKGYRVYNLKTQT 915
+F N T D VFG CY K DP++ K +FLG+ T + GY V +L T
Sbjct: 164 QFLNNDSPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDLPTTX 343
Query: 916 V 916
+
Sbjct: 344 I 346
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,242,330
Number of Sequences: 28460
Number of extensions: 310796
Number of successful extensions: 1707
Number of sequences better than 10.0: 78
Number of HSP's better than 10.0 without gapping: 1658
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1695
length of query: 1523
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1421
effective length of database: 1,994,680
effective search space: 2834440280
effective search space used: 2834440280
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144724.6