BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144722.9 + phase: 0
(259 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP044919 248 7e-67
BP041494 158 2e-58
BP040870 206 4e-54
TC9795 homologue to UP|Q9ATB8 (Q9ATB8) BiP-isoform D (Fragment),... 204 1e-53
TC18168 homologue to UP|Q8GSN4 (Q8GSN4) Non-cell-autonomous heat... 201 8e-53
AW720237 190 2e-49
TC8353 homologue to UP|HS7M_PHAVU (Q01899) Heat shock 70 kDa pro... 138 1e-33
AV769455 117 3e-27
BP083571 112 8e-26
AW428869 65 2e-11
TC14233 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (67%) 56 5e-09
BP064898 53 6e-08
TC14234 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (66%) 28 0.042
TC10576 similar to UP|Q93ZQ3 (Q93ZQ3) AT3g63200/F16M2_50, partia... 29 0.92
AU251419 27 3.5
AV409242 26 6.0
AV406566 26 7.8
>BP044919
Length = 519
Score = 248 bits (633), Expect = 7e-67
Identities = 119/164 (72%), Positives = 143/164 (86%)
Frame = +3
Query: 80 GRKFSDPVVQKDIMFWPFKVISGLNDKPMIAVKYKGQEKNLCAEEISSIILANMKKIAEA 139
GRK+SD V+Q+D+ WPFKVI+G +DKPMI VK+KG+EK L EEISS++L+ MK+IAEA
Sbjct: 27 GRKYSDSVIQEDVKLWPFKVIAGTDDKPMIVVKHKGEEKKLVPEEISSMVLSKMKEIAEA 206
Query: 140 YLKSPVKNAVVTVPAYFNDSQRKATMNAGAIAGLNVMRIINDPTAAAIAYGLDNKGRCDG 199
+L+S VKNAVVTVPAYFND+QRKAT +AG IAGLNVMRIIN+PTAAA+AYGL + G
Sbjct: 207 FLESSVKNAVVTVPAYFNDAQRKATKDAGTIAGLNVMRIINEPTAAALAYGLQKRANLVG 386
Query: 200 ERNIFVFDLGGGTFDVSLLTIKGDVFEVKATAGNTHLGGEDFDN 243
ERN+F+FDLGGGTFDVSLLTIK D FEVKAT+G+THLG +DFDN
Sbjct: 387 ERNVFIFDLGGGTFDVSLLTIKDDNFEVKATSGDTHLGXKDFDN 518
>BP041494
Length = 572
Score = 158 bits (399), Expect(2) = 2e-58
Identities = 79/110 (71%), Positives = 90/110 (81%)
Frame = +3
Query: 74 DAKRLIGRKFSDPVVQKDIMFWPFKVISGLNDKPMIAVKYKGQEKNLCAEEISSIILANM 133
DAKRLIGRKFSD V+ D+ WPFKVISG D+PMI V YK +EK AEEISS++L M
Sbjct: 243 DAKRLIGRKFSDDSVESDMKLWPFKVISGPVDRPMIVVNYKNEEKQFTAEEISSMVLIKM 422
Query: 134 KKIAEAYLKSPVKNAVVTVPAYFNDSQRKATMNAGAIAGLNVMRIINDPT 183
++IAEAYL S VKN VVTVPAYFNDSQR+AT +AG IAGLNVMRIIN+PT
Sbjct: 423 REIAEAYLGSTVKNVVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPT 572
Score = 84.0 bits (206), Expect(2) = 2e-58
Identities = 40/63 (63%), Positives = 51/63 (80%)
Frame = +1
Query: 1 MARKHDGHAVGIDLGTTYSCVGVWLEEKNRVEIIHNDEGNKITPSFVAFTDDQRLVGGAA 60
M + + A+GIDLGTTYSCVGVW + +RVEII ND+GN+ TPS+VAFTD +RL+G AA
Sbjct: 61 MVGEGESPAIGIDLGTTYSCVGVW--QNDRVEIIPNDQGNRTTPSYVAFTDFERLIGDAA 234
Query: 61 KDQ 63
K+Q
Sbjct: 235 KNQ 243
>BP040870
Length = 505
Score = 206 bits (523), Expect = 4e-54
Identities = 100/135 (74%), Positives = 117/135 (86%)
Frame = +2
Query: 111 VKYKGQEKNLCAEEISSIILANMKKIAEAYLKSPVKNAVVTVPAYFNDSQRKATMNAGAI 170
V YKG+EK AEEISS++L MK+IAEAYL + +KNAVVTVPAYFNDSQR+AT +AG I
Sbjct: 5 VNYKGEEKQFSAEEISSMVLMKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVI 184
Query: 171 AGLNVMRIINDPTAAAIAYGLDNKGRCDGERNIFVFDLGGGTFDVSLLTIKGDVFEVKAT 230
+GLNVMRIIN+PTAAAIAYGLD K GE+N+ +FDLGGGTFDVSLLTI+ +FEVK+T
Sbjct: 185 SGLNVMRIINEPTAAAIAYGLDKKATSTGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKST 364
Query: 231 AGNTHLGGEDFDNRM 245
AG+THLGGEDFDNRM
Sbjct: 365 AGDTHLGGEDFDNRM 409
>TC9795 homologue to UP|Q9ATB8 (Q9ATB8) BiP-isoform D (Fragment), partial
(63%)
Length = 981
Score = 204 bits (519), Expect = 1e-53
Identities = 105/178 (58%), Positives = 135/178 (74%), Gaps = 1/178 (0%)
Frame = +1
Query: 7 GHAVGIDLGTTYSCVGVWLEEKNRVEIIHNDEGNKITPSFVAFTDDQRLVGGAAKDQAAI 66
G +GIDLGTTYSCVGV+ VEII ND+GN+ITPS+VAF D +RL+G AAK+Q A+
Sbjct: 145 GTVIGIDLGTTYSCVGVY--RNGHVEIIANDQGNRITPSWVAFNDGERLIGEAAKNQFAV 318
Query: 67 NPQNTVFDAKRLIGRKFSDPVVQKDIMFWPFKVISGLNDKPMIAVKYK-GQEKNLCAEEI 125
NP+ T+FD KRLIGRKF D VQ+D+ P+K+++ + KP I VK K G+ K EE+
Sbjct: 319 NPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVN-KDGKPYIQVKIKDGETKVFSPEEV 495
Query: 126 SSIILANMKKIAEAYLKSPVKNAVVTVPAYFNDSQRKATMNAGAIAGLNVMRIINDPT 183
S++IL MK+ AEA+L + +AVVTVPAYFND+QR+AT +AG IAGLNV RIIN+PT
Sbjct: 496 SAMILTKMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPT 669
Score = 96.7 bits (239), Expect = 4e-21
Identities = 53/86 (61%), Positives = 63/86 (72%)
Frame = +2
Query: 173 LNVMRIINDPTAAAIAYGLDNKGRCDGERNIFVFDLGGGTFDVSLLTIKGDVFEVKATAG 232
L ++ + +P AAAIAYGLD KG GE+NI VFDLGGGTFDVS+LTI VFEV AT G
Sbjct: 638 LMLLELSMNPPAAAIAYGLDKKG---GEKNILVFDLGGGTFDVSILTIDNGVFEVLATNG 808
Query: 233 NTHLGGEDFDNRMCWNKMCFTMNLIK 258
+THLGGEDFD R+ M + + LIK
Sbjct: 809 DTHLGGEDFDQRI----MEYFIKLIK 874
>TC18168 homologue to UP|Q8GSN4 (Q8GSN4) Non-cell-autonomous heat shock
cognate protein 70, partial (23%)
Length = 508
Score = 201 bits (512), Expect = 8e-53
Identities = 101/152 (66%), Positives = 122/152 (79%)
Frame = +2
Query: 1 MARKHDGHAVGIDLGTTYSCVGVWLEEKNRVEIIHNDEGNKITPSFVAFTDDQRLVGGAA 60
MA K +G A+GIDLGTTYSCVGVW + +RVEII ND+GN+ TPS+VAFTD +RL+G AA
Sbjct: 59 MAGKGEGPAIGIDLGTTYSCVGVW--QHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAA 232
Query: 61 KDQAAINPQNTVFDAKRLIGRKFSDPVVQKDIMFWPFKVISGLNDKPMIAVKYKGQEKNL 120
K+Q A+NP NTVFDAKRLIGR+ SD VQ D+ WPFKV SG +KPMI V YKG++K
Sbjct: 233 KNQVAMNPINTVFDAKRLIGRRVSDASVQSDMKLWPFKVNSGPAEKPMIQVSYKGEDKQF 412
Query: 121 CAEEISSIILANMKKIAEAYLKSPVKNAVVTV 152
AEEISS++L M++IAEAYL S +KNAVVTV
Sbjct: 413 AAEEISSMVLMKMREIAEAYLGSTIKNAVVTV 508
>AW720237
Length = 459
Score = 190 bits (482), Expect = 2e-49
Identities = 91/141 (64%), Positives = 113/141 (79%)
Frame = +3
Query: 9 AVGIDLGTTYSCVGVWLEEKNRVEIIHNDEGNKITPSFVAFTDDQRLVGGAAKDQAAINP 68
A+GIDLGTTYSCVGVW+ + RVEII ND+GN+ TPS+V FT+ +RL+G AAK+Q A NP
Sbjct: 42 AIGIDLGTTYSCVGVWMND--RVEIIANDQGNRTTPSYVGFTESERLIGDAAKNQVARNP 215
Query: 69 QNTVFDAKRLIGRKFSDPVVQKDIMFWPFKVISGLNDKPMIAVKYKGQEKNLCAEEISSI 128
NTVFDAKRLIGRKF DP+VQKDI WPFKV +G + KP+IAVKYKG+ K AE++SS+
Sbjct: 216 INTVFDAKRLIGRKFDDPIVQKDIKLWPFKVEAGPDGKPLIAVKYKGETKKFHAEQVSSM 395
Query: 129 ILANMKKIAEAYLKSPVKNAV 149
+L MK+ AEAYL +K+ V
Sbjct: 396 VLTKMKETAEAYLNKTIKDIV 458
>TC8353 homologue to UP|HS7M_PHAVU (Q01899) Heat shock 70 kDa protein,
mitochondrial precursor, partial (74%)
Length = 1752
Score = 138 bits (347), Expect = 1e-33
Identities = 72/109 (66%), Positives = 84/109 (77%)
Frame = +3
Query: 137 AEAYLKSPVKNAVVTVPAYFNDSQRKATMNAGAIAGLNVMRIINDPTAAAIAYGLDNKGR 196
AEAYL V AV+TVPAYFND+ R+AT +AG IAGL+V RIIN+PTAAA++YG+ NK
Sbjct: 9 AEAYLGKSVSKAVITVPAYFNDAHRQATKDAGRIAGLDVQRIINEPTAAALSYGMTNK-- 182
Query: 197 CDGERNIFVFDLGGGTFDVSLLTIKGDVFEVKATAGNTHLGGEDFDNRM 245
E I VFDLGGGTFDVS+L I VFEVKAT G+T LGGEDFDN +
Sbjct: 183 ---EGLIAVFDLGGGTFDVSILEISNGVFEVKATNGDTFLGGEDFDNAL 320
>AV769455
Length = 556
Score = 117 bits (292), Expect = 3e-27
Identities = 60/184 (32%), Positives = 106/184 (57%), Gaps = 1/184 (0%)
Frame = +1
Query: 56 VGGAAKDQAAINPQNTVFDAKRLIGRKFSDPVVQKDIMFWPFKVISGLNDKPMIAVKYKG 115
+G A +NP+N++ KRLIGR+F+DP +Q+D+ PF V G + P+I +Y G
Sbjct: 4 LGTAGASSTMMNPKNSISQLKRLIGRQFADPELQRDLKSLPFAVTEGPDGFPLIHARYLG 183
Query: 116 QEKNLCAEEISSIILANMKKIAEAYLKSPVKNAVVTVPAYFNDSQRKATMNAGAIAGLNV 175
+ + ++ ++L+N+K+IA+ L + V + + +P YF D QR+A ++A IAGL+
Sbjct: 184 ETRTFTPTQVFGMMLSNLKEIAQKNLNAAVVDCCIGIPIYFTDLQRRAVLDAATIAGLHP 363
Query: 176 MRIINDPTAAAIAYGLDNKGRCDGE-RNIFVFDLGGGTFDVSLLTIKGDVFEVKATAGNT 234
+R+ ++ TA A+AYG+ + + N+ D+G + V + K +V A + +
Sbjct: 364 LRLFHETTATALAYGIYKTDLPENDPLNVAFVDVGHASMQVCIAGYKKGQLKVLAHSYDR 543
Query: 235 HLGG 238
LGG
Sbjct: 544 SLGG 555
>BP083571
Length = 423
Score = 112 bits (279), Expect = 8e-26
Identities = 51/72 (70%), Positives = 61/72 (83%)
Frame = +1
Query: 1 MARKHDGHAVGIDLGTTYSCVGVWLEEKNRVEIIHNDEGNKITPSFVAFTDDQRLVGGAA 60
MA K++G A+GIDLGTTYSCV VW E+ +R EIIHN++GN+ TPSFVAFTD QRL+G AA
Sbjct: 145 MATKYEGPAIGIDLGTTYSCVAVWQEQNDRAEIIHNEQGNRTTPSFVAFTDTQRLIGDAA 324
Query: 61 KDQAAINPQNTV 72
K+QAA NP NTV
Sbjct: 325 KNQAATNPTNTV 360
>AW428869
Length = 181
Score = 64.7 bits (156), Expect = 2e-11
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = +1
Query: 203 IFVFDLGGGTFDVSLLTIKGDVFEVKATAGNTHLGGEDFDNRM 245
I VFDLGGGTFDVS+L + VFEV +T+G+THLGG+DFD R+
Sbjct: 22 ILVFDLGGGTFDVSVLEVGDGVFEVLSTSGDTHLGGDDFDKRI 150
>TC14233 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (67%)
Length = 1570
Score = 56.2 bits (134), Expect = 5e-09
Identities = 26/31 (83%), Positives = 29/31 (92%)
Frame = +1
Query: 215 VSLLTIKGDVFEVKATAGNTHLGGEDFDNRM 245
VSLLTI+ +FEVKATAG+THLGGEDFDNRM
Sbjct: 1 VSLLTIEEGIFEVKATAGDTHLGGEDFDNRM 93
>BP064898
Length = 542
Score = 52.8 bits (125), Expect = 6e-08
Identities = 24/66 (36%), Positives = 42/66 (63%)
Frame = +1
Query: 76 KRLIGRKFSDPVVQKDIMFWPFKVISGLNDKPMIAVKYKGQEKNLCAEEISSIILANMKK 135
KRLIG +FSDP +Q+D+ PF V G + P+I +Y G+ + ++ +++L+N+K+
Sbjct: 337 KRLIGIQFSDPQLQRDLKSLPFLVTEGPDGYPLIHARYLGETRTFTPTQVFAMMLSNLKE 516
Query: 136 IAEAYL 141
I + L
Sbjct: 517 IXQKNL 534
>TC14234 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (66%)
Length = 1528
Score = 27.7 bits (60), Expect(2) = 0.042
Identities = 11/13 (84%), Positives = 13/13 (99%)
Frame = +1
Query: 224 VFEVKATAGNTHL 236
+FEVKATAG+THL
Sbjct: 10 IFEVKATAGDTHL 48
Score = 24.3 bits (51), Expect(2) = 0.042
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +3
Query: 237 GGEDFDNRM 245
GGEDFDNRM
Sbjct: 48 GGEDFDNRM 74
>TC10576 similar to UP|Q93ZQ3 (Q93ZQ3) AT3g63200/F16M2_50, partial (39%)
Length = 751
Score = 28.9 bits (63), Expect = 0.92
Identities = 23/90 (25%), Positives = 39/90 (42%), Gaps = 18/90 (20%)
Frame = +2
Query: 178 IINDPTAAAIAYGLDNK---GRCDGERNIFVFDLGGGTFDV---------------SLLT 219
++N+PTAAA+ + L NK +G ++ V LG G+ + S +
Sbjct: 119 VMNNPTAAAVTHVLHNKRDFPAVNGVEDLLVLSLGNGSSNAKACENEKEKENHRTCSTPS 298
Query: 220 IKGDVFEVKATAGNTHLGGEDFDNRMCWNK 249
+ G V + + + LG N CWN+
Sbjct: 299 VVGIVLDGVSETVDQMLG-----NAFCWNR 373
>AU251419
Length = 404
Score = 26.9 bits (58), Expect = 3.5
Identities = 17/71 (23%), Positives = 32/71 (44%), Gaps = 3/71 (4%)
Frame = +2
Query: 145 VKNAVVTVPAYFNDSQRKATMNAGAIAGLNVMRIINDPTAAAIAYGLDNK---GRCDGER 201
V A P F Q + + + ++ ++N+P AAA+ + L NK +G
Sbjct: 89 VCRATSATPGVFKPFQFNSIDGRTSCSAVDGGLVMNNPAAAAVTHVLHNKRDFPSVNGVE 268
Query: 202 NIFVFDLGGGT 212
++ V +G G+
Sbjct: 269 DLLVLSIGNGS 301
>AV409242
Length = 315
Score = 26.2 bits (56), Expect = 6.0
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -2
Query: 194 KGRCDGERNIFVFDLGGG 211
+GRCDG R+I + DL GG
Sbjct: 194 RGRCDGCRSISLPDLDGG 141
>AV406566
Length = 376
Score = 25.8 bits (55), Expect = 7.8
Identities = 8/17 (47%), Positives = 14/17 (82%)
Frame = +3
Query: 11 GIDLGTTYSCVGVWLEE 27
G+D+GTT++ + WLE+
Sbjct: 171 GMDIGTTFTAITGWLED 221
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.137 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,831,544
Number of Sequences: 28460
Number of extensions: 42258
Number of successful extensions: 203
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of query: 259
length of database: 4,897,600
effective HSP length: 88
effective length of query: 171
effective length of database: 2,393,120
effective search space: 409223520
effective search space used: 409223520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144722.9