BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144722.14 - phase: 0
(220 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP084332 64 2e-11
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 58 1e-09
BP075943 52 6e-08
TC9992 51 2e-07
BP043850 50 2e-07
BP057233 49 5e-07
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 39 2e-06
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 47 3e-06
AV780009 46 6e-06
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 44 3e-05
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 42 1e-04
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 40 2e-04
AV424544 34 0.017
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 32 0.086
TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retro... 30 0.33
BP084259 30 0.43
AV779990 29 0.73
AW720203 29 0.73
TC9993 27 3.6
>BP084332
Length = 368
Score = 64.3 bits (155), Expect = 2e-11
Identities = 32/96 (33%), Positives = 51/96 (52%)
Frame = +2
Query: 121 LAATTAVNQALWLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFL 180
++A Q LW++ L D + + + DN A I++SKN + H + KH +K F+
Sbjct: 71 ISAAICSTQMLWMKHQLEDYQILE-SNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFI 247
Query: 181 RDVQKDGDVCLKYCKTEDQLSYIFTKALPTGRFELL 216
RD + G + LK+ T+ Q + IFTK L RF +
Sbjct: 248 RDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFI 355
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 57.8 bits (138), Expect = 1e-09
Identities = 33/73 (45%), Positives = 47/73 (64%), Gaps = 2/73 (2%)
Frame = -1
Query: 32 QRKYANEMLKKFNMENRKSMSTPMCPKERLC--KDVEEQVDETLYQSLIGCLMYLTTTRS 89
Q+ Y +++LKKF M N K +ST + KE ++ ++VD T Y+SLIG + YL T RS
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 90 DILYDVNVLSRFM 102
DI+ V + SRFM
Sbjct: 559 DIVCGVGLRSRFM 521
>BP075943
Length = 547
Score = 52.4 bits (124), Expect = 6e-08
Identities = 35/114 (30%), Positives = 54/114 (46%), Gaps = 15/114 (13%)
Frame = -3
Query: 5 FEMSDLGEMSYFLVIEVEQKNGDIFICQRKYANEMLKK---FNMENR------------K 49
F + DLGE YFL +E+ + I + QRKYA +++ F + R
Sbjct: 437 FRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQLISDSGHFGFQPRFYSHGTNSQTLGT 258
Query: 50 SMSTPMCPKERLCKDVEEQVDETLYQSLIGCLMYLTTTRSDILYDVNVLSRFMN 103
+ TP+ D Y+ ++G L+YL TTR DI + VN LS+F++
Sbjct: 257 NTGTPL-------------TDIGSYRRIVGRLLYLNTTRPDITFAVNQLSQFLS 135
>TC9992
Length = 508
Score = 50.8 bits (120), Expect = 2e-07
Identities = 26/64 (40%), Positives = 38/64 (58%)
Frame = +3
Query: 121 LAATTAVNQALWLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFL 180
L T VN LW++K+L DL +E E + VDN A I+IS + +F KT I+ +FL
Sbjct: 51 LLPATVVNPPLWIQKLLLDLPMEPMEHPKFFVDN*AIISISAHPLFQRKTHPSKIQFYFL 230
Query: 181 RDVQ 184
R+ +
Sbjct: 231 REAE 242
>BP043850
Length = 515
Score = 50.4 bits (119), Expect = 2e-07
Identities = 27/92 (29%), Positives = 47/92 (50%)
Frame = -1
Query: 128 NQALWLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFLRDVQKDG 187
++ +WLR +L++L Q + T + DN + I I+ N V+H T+H + +R+
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 188 DVCLKYCKTEDQLSYIFTKALPTGRFELLREK 219
+ L + T Q++ I TK+L R L K
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSK 240
>BP057233
Length = 473
Score = 49.3 bits (116), Expect = 5e-07
Identities = 24/67 (35%), Positives = 37/67 (54%)
Frame = -2
Query: 153 DNHATIAISKNHVFHGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQLSYIFTKALPTGR 212
DN + A++ N V H ++KH I + ++RD +V + Y T DQ++ TK L R
Sbjct: 436 DNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLSHTR 257
Query: 213 FELLREK 219
F LR+K
Sbjct: 256 FSQLRDK 236
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein,
partial (4%)
Length = 528
Score = 38.5 bits (88), Expect(2) = 2e-06
Identities = 20/48 (41%), Positives = 27/48 (55%)
Frame = +3
Query: 170 TKHFSIKLFFLRDVQKDGDVCLKYCKTEDQLSYIFTKALPTGRFELLR 217
T + K FLRD G + LK+C T+ Q++ I TK L T RF +R
Sbjct: 63 TLNIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMR 206
Score = 28.5 bits (62), Expect(2) = 2e-06
Identities = 11/21 (52%), Positives = 16/21 (75%)
Frame = +2
Query: 152 VDNHATIAISKNHVFHGKTKH 172
VDN + I ++KN V HG++KH
Sbjct: 2 VDNKSAIDLAKNPVSHGRSKH 64
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 47.0 bits (110), Expect = 3e-06
Identities = 28/88 (31%), Positives = 44/88 (49%)
Frame = +1
Query: 132 WLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFLRDVQKDGDVCL 191
WL +L DL + + V DN++ I+ N VFH +TKH I +R+ + G + L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 192 KYCKTEDQLSYIFTKALPTGRFELLREK 219
+ + L+ I+TKAL F + K
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAK 267
>AV780009
Length = 529
Score = 45.8 bits (107), Expect = 6e-06
Identities = 33/98 (33%), Positives = 45/98 (45%)
Frame = -1
Query: 119 RVLAATTAVNQALWLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLF 178
R LAAT V + WL + L L + DN + + I+ N FH +TKH +
Sbjct: 298 RALAAT--VCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNPTFHERTKHIELDCH 125
Query: 179 FLRDVQKDGDVCLKYCKTEDQLSYIFTKALPTGRFELL 216
+R + G + L T QL+ IFTK P F LL
Sbjct: 124 VVRAKLQAGLIHLLPISTHHQLADIFTKPCPHLCFPLL 11
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 43.5 bits (101), Expect = 3e-05
Identities = 24/88 (27%), Positives = 48/88 (54%), Gaps = 9/88 (10%)
Frame = +2
Query: 5 FEMSDLGEMSYFLVIEV--EQKNGDIFICQRKYANEMLKKFNMENRKSMSTPMCPKERLC 62
F+M DLG + L +++ ++K+ I++ Q+ Y ++L++FNM++ +STP+ +L
Sbjct: 179 FDMKDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLS 358
Query: 63 KDV-------EEQVDETLYQSLIGCLMY 83
+ ++ Y S +G LMY
Sbjct: 359 SSMIPSSEAERMEMSRVPYASAVGSLMY 442
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 41.6 bits (96), Expect = 1e-04
Identities = 22/80 (27%), Positives = 40/80 (49%)
Frame = +3
Query: 129 QALWLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFLRDVQKDGD 188
+ALWL L DL + + DN + + ++ N VFH +T++ I + G
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 189 VCLKYCKTEDQLSYIFTKAL 208
+ L + + DQ++ +FTK +
Sbjct: 201 LHLLHVPSSDQVADVFTKTI 260
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 40.4 bits (93), Expect = 2e-04
Identities = 27/88 (30%), Positives = 41/88 (45%)
Frame = +1
Query: 132 WLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFLRDVQKDGDVCL 191
WL +L DL + + + D+ + I+ N VFH +TKH I +R+ + L
Sbjct: 13 WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHL 192
Query: 192 KYCKTEDQLSYIFTKALPTGRFELLREK 219
+ DQ + I TK L +G F L K
Sbjct: 193 LPISSVDQTADILTKPLESGPFSHLVSK 276
>AV424544
Length = 276
Score = 34.3 bits (77), Expect = 0.017
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +3
Query: 5 FEMSDLGEMSYFLVIEVEQKNGDIFICQRKY 35
F + DLG + YFL +EV + + +F+ QRKY
Sbjct: 183 FRIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 32.0 bits (71), Expect = 0.086
Identities = 22/95 (23%), Positives = 42/95 (44%)
Frame = +3
Query: 121 LAATTAVNQALWLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFL 180
+AAT A ++ LW++ L + +V A +++ +F + L
Sbjct: 87 IAATEACHELLWMKNFLQNAWFHSHPILCCIVITKALFTLARILLFIQDPSTLMFVIIGL 266
Query: 181 RDVQKDGDVCLKYCKTEDQLSYIFTKALPTGRFEL 215
RDV + L+ T+D + + TK+LP + E+
Sbjct: 267 RDVLNSKLLELEKIHTDDDGADMMTKSLPREKLEV 371
>TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement pol
polyprotein, partial (3%)
Length = 517
Score = 30.0 bits (66), Expect = 0.33
Identities = 18/55 (32%), Positives = 29/55 (52%)
Frame = -2
Query: 153 DNHATIAISKNHVFHGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQLSYIFTKA 207
DN A + + K F + KH I FL D + D+ ++ + DQL+ +FTK+
Sbjct: 354 DNKAALHMPKI*-FSMRAKHIEIDFHFL*DRRLYLDISTRFVNSNDQLTDVFTKS 193
>BP084259
Length = 385
Score = 29.6 bits (65), Expect = 0.43
Identities = 16/62 (25%), Positives = 30/62 (47%)
Frame = -3
Query: 118 SRVLAATTAVNQALWLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKL 177
+R + +T A + LWL+++L +L + + +N + N VFH KH ++
Sbjct: 182 TRTIGSTAA--EFLWLQQLLKELQFSLPKVPCIFSNNINATYVCLNLVFHSHMKHVALDC 9
Query: 178 FF 179
F
Sbjct: 8 HF 3
>AV779990
Length = 542
Score = 28.9 bits (63), Expect = 0.73
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +3
Query: 153 DNHATIAISKNHVFHGKTKH 172
DNHA + IS N + H +TKH
Sbjct: 312 DNHAALDISSNPISHERTKH 371
>AW720203
Length = 303
Score = 28.9 bits (63), Expect = 0.73
Identities = 22/66 (33%), Positives = 30/66 (45%), Gaps = 11/66 (16%)
Frame = +3
Query: 102 MNCAKESLFKGAKRVLSRVL----AATTAVNQALWLRKVLTDLHLEQRE-------TTEV 150
+ CA+E + K S A+TTA +W+ VL+DL RE TT V
Sbjct: 36 LRCAEEGSWLDLKHKPSTETHVGEASTTATRSPIWVPSVLSDLSSSSREKMHWWLMTTSV 215
Query: 151 MVDNHA 156
VD+ A
Sbjct: 216 GVDDGA 233
>TC9993
Length = 408
Score = 26.6 bits (57), Expect = 3.6
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +3
Query: 165 VFHGKTKHFSIKLFFLRDVQ 184
+F KTKH IK +FLR+ +
Sbjct: 3 LFQRKTKHSKIKFYFLREAE 62
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.136 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,361,168
Number of Sequences: 28460
Number of extensions: 39497
Number of successful extensions: 288
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 288
length of query: 220
length of database: 4,897,600
effective HSP length: 87
effective length of query: 133
effective length of database: 2,421,580
effective search space: 322070140
effective search space used: 322070140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144722.14