BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144722.10 + phase: 0 /pseudo
(1595 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 103 2e-22
BP084332 63 3e-10
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 36 0.054
AV773507 35 0.092
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 35 0.12
AV779081 32 0.78
TC8736 similar to PIR|T06396|T06396 isoprenylated protein - soyb... 32 1.0
TC18665 similar to UP|O23061 (O23061) BAC IG005I10, partial (65%) 32 1.0
TC17386 similar to UP|Q9LU46 (Q9LU46) DEAD-box protein abstrakt,... 32 1.0
TC9100 similar to UP|Q8W544 (Q8W544) NADH-ubiquinone oxidoreduct... 32 1.0
TC8074 similar to UP|AAQ72789 (AAQ72789) 60S ribosomal protein L... 32 1.0
TC10133 similar to UP|Q9LSS5 (Q9LSS5) Myosin heavy chain-like, p... 32 1.0
BP039068 31 1.7
AV421607 31 1.7
TC15740 weakly similar to GB|AAM19935.1|20453393|AY097419 AT5g58... 30 2.3
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 30 2.3
BP055887 30 2.3
TC15523 similar to UP|GRPA_MAIZE (P10979) Glycine-rich RNA-bindi... 30 3.0
TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protei... 30 3.0
AV780703 30 3.9
>BP066094
Length = 532
Score = 103 bits (258), Expect = 2e-22
Identities = 69/142 (48%), Positives = 89/142 (62%)
Frame = +3
Query: 1176 KQSGYFYPTQLIMA*YYIKWMSKVPFLMVSLKKKCMLNNLLGLRILSILTMFINLRNHYM 1235
K S Y +Q I *++I+ M +VPF MV+ ++K M N ++ LT+F N RN M
Sbjct: 96 KLSDY*SHSQ*ITT*FFIR*MLRVPF*MVTSQRKSMFINPQVXKMRRTLTIFSN*RNLSM 275
Query: 1236 A*NKLPELGMID*VIS*LKMILKEDKLTQHSSEGLLRKIF*LCKYMLMI*YLVLLMHLFA 1295
*+KLPE GM D S KM K Q S L + I+*LCK+MLMI YL LL+HL+A
Sbjct: 276 V*SKLPEHGMRDSAHSFWKMSX*GVK*IQLSFAKLTKMIY*LCKFMLMILYLDLLIHLYA 455
Query: 1296 NNFLS*CRMNLK*V*WEN*NSF 1317
+FL *CR+NLK V*WEN N+F
Sbjct: 456 KSFLR*CRLNLKCV*WENSNTF 521
>BP084332
Length = 368
Score = 63.2 bits (152), Expect = 3e-10
Identities = 48/116 (41%), Positives = 62/116 (53%)
Frame = +3
Query: 1473 QAKDKQLLLCLQQKQNTFQLQVVAHNYFG*NISWKTIRLMLTVFPFIVIILLLFVCQRIQ 1532
Q + Q L L Q+QN Q Q A G*NISW+ I+ + + IVI LL F RI
Sbjct: 21 QLRGNQPLHYLLQRQNISQQQYAALRCSG*NISWRIIKSLRAISQSIVITLLQFH*VRIL 200
Query: 1533 FYIQEPSILKSNTILSETMFKKGF*IYNSLILNINGLIYLQNLYLLKDLILLRKI* 1588
YIQ S L+ + L E M+++ + + LIL ING I LQ+ DLI KI*
Sbjct: 201 SYIQGQSTLR*SITLLEIMYRRAYFF*SLLILTINGQISLQSP*QRIDLISF*KI* 368
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 35.8 bits (81), Expect = 0.054
Identities = 16/45 (35%), Positives = 28/45 (61%), Gaps = 8/45 (17%)
Frame = +2
Query: 149 KKFLSKRGSYKNS--KNEDQKG------CFNCKKPGHFIADCPDL 185
+K S R SY+++ + + ++G C NCK+PGHF +CP++
Sbjct: 260 RKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFARECPNV 394
Score = 31.2 bits (69), Expect = 1.3
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +2
Query: 169 CFNCKKPGHFIADCPD 184
C+NCK+PGH + CP+
Sbjct: 458 CWNCKEPGHMASSCPN 505
>AV773507
Length = 496
Score = 35.0 bits (79), Expect = 0.092
Identities = 14/29 (48%), Positives = 15/29 (51%)
Frame = +2
Query: 155 RGSYKNSKNEDQKGCFNCKKPGHFIADCP 183
RGSY Q CF C +PGH DCP
Sbjct: 308 RGSYGAGDRVGQDDCFECGRPGHRARDCP 394
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 34.7 bits (78), Expect = 0.12
Identities = 15/36 (41%), Positives = 20/36 (54%)
Frame = +2
Query: 158 YKNSKNEDQKGCFNCKKPGHFIADCPDLQKEKFKGK 193
Y S+ + CF C KPGHF +CP + E +GK
Sbjct: 386 YGGSRGSNGGECFKCGKPGHFARECPS-EGETRRGK 490
>AV779081
Length = 467
Score = 32.0 bits (71), Expect = 0.78
Identities = 21/82 (25%), Positives = 36/82 (43%), Gaps = 3/82 (3%)
Frame = -2
Query: 374 TYKNKGKGIGYSEEKSKEYSLKSYCDCIKDGL---KSTFVPEGTNAITAVQSKPEASGSQ 430
T +K Y+ S S D + L +S VP +TA+ S PEA+G++
Sbjct: 460 TASSKTLAASYNAWTSPSQKSSSSSDLVNFNLSFSRSVLVPASGLPLTAIPSSPEANGNR 281
Query: 431 AKITSKPENLKIKVMTKSDPKS 452
+ EN++ + + P+S
Sbjct: 280 CTLLLGLENIR*NFLQPTKPRS 215
>TC8736 similar to PIR|T06396|T06396 isoprenylated protein - soybean
(fragment) {Glycine max;}, partial (84%)
Length = 1268
Score = 31.6 bits (70), Expect = 1.0
Identities = 26/104 (25%), Positives = 43/104 (41%), Gaps = 9/104 (8%)
Frame = +2
Query: 433 ITSKPENLKIKVMTKSDPKSQKIKILKRSEPVHQNLIKPESKIPKQKDQKNKAATASEKT 492
+ PE ++ K+ K + I+I++ L KP+ P++K + +K A +
Sbjct: 377 VCCSPEKIRDKLCCKGGGTIKSIEIVE--------LPKPKPPEPEKKKEADKPKAAEHEK 532
Query: 493 IPKGVKPKVL-------NDQKPLSIHP--KVQGRKSKTSKINPK 527
G KPK + +KP + P K G K K PK
Sbjct: 533 KKDGEKPKAAAEPEKKKDSEKPKAAEPEKKKDGDKEKPKSDGPK 664
>TC18665 similar to UP|O23061 (O23061) BAC IG005I10, partial (65%)
Length = 451
Score = 31.6 bits (70), Expect = 1.0
Identities = 20/54 (37%), Positives = 24/54 (44%)
Frame = -2
Query: 217 EDLDSESGSDKEEADDDAKAAVGLVATVSSEAVSEAESDSEDENEVYSKIPRQE 270
EDLDS+ D+E DDD EA EA DE+EV + P E
Sbjct: 189 EDLDSDGFRDEEIGDDDEGE-----GEEEDEATEEASGFDGDEHEVRERAPGDE 43
>TC17386 similar to UP|Q9LU46 (Q9LU46) DEAD-box protein abstrakt, partial
(19%)
Length = 507
Score = 31.6 bits (70), Expect = 1.0
Identities = 17/46 (36%), Positives = 23/46 (49%), Gaps = 2/46 (4%)
Frame = +2
Query: 167 KGCFNCKKPGHFIADCPDLQKEKFK--GKSKKSSFNSSKFRKQIKK 210
KGC C GH I DCP L+ +K ++ F S +R +I K
Sbjct: 203 KGCAYCGGLGHRIRDCPKLEHQKSMAIANNRHDYFGSGGYRGEI*K 340
>TC9100 similar to UP|Q8W544 (Q8W544) NADH-ubiquinone oxidoreductase
(Fragment), partial (97%)
Length = 712
Score = 31.6 bits (70), Expect = 1.0
Identities = 19/41 (46%), Positives = 23/41 (55%)
Frame = -3
Query: 276 KELLSLFEHRTNELTDLKEKYVDLMKQQKSTLLELKASEEE 316
+ LLSL TN L KEKY+ + KQQ S + E A E E
Sbjct: 644 ERLLSLKLLNTNNLLWRKEKYIQIEKQQSSNVAEDHAFEGE 522
>TC8074 similar to UP|AAQ72789 (AAQ72789) 60S ribosomal protein L5, partial
(96%)
Length = 1086
Score = 31.6 bits (70), Expect = 1.0
Identities = 16/52 (30%), Positives = 31/52 (58%), Gaps = 1/52 (1%)
Frame = +3
Query: 183 PDLQKEKFKGKSKKSSFNSSKFRKQIKKS-LMATWEDLDSESGSDKEEADDD 233
P ++K + K K +N K + +K+ L+A + L+S +G+D++E DD+
Sbjct: 744 PTIKKAEKKPPKKSKRYNLKKLTYEERKAKLIARLQALNSAAGADEDEEDDE 899
>TC10133 similar to UP|Q9LSS5 (Q9LSS5) Myosin heavy chain-like, partial
(34%)
Length = 1827
Score = 31.6 bits (70), Expect = 1.0
Identities = 30/132 (22%), Positives = 55/132 (40%)
Frame = +1
Query: 209 KKSLMATWEDLDSESGSDKEEADDDAKAAVGLVATVSSEAVSEAESDSEDENEVYSKIPR 268
+ L A+ + L +S + A++ V L + E+V ++S D +
Sbjct: 292 QSELEASQKHLSVDSAALASAANEIQLLKVQLELVANCESVQTHHAESAD-------VEL 450
Query: 269 QELVDSLKELLSLFEHRTNELTDLKEKYVDLMKQQKSTLLELKASEEELKGFNLISTTYE 328
L +L E LSL E+ N+L + KE TLL+L+ ++ ++
Sbjct: 451 LNLKQNLSETLSLVENMKNQLRNFKEFEAQAQGLVSETLLQLETAKRTVEILRGDVAKSV 630
Query: 329 DRLKSLCQKLQE 340
D KS+ +L +
Sbjct: 631 DGYKSIALELDQ 666
>BP039068
Length = 467
Score = 30.8 bits (68), Expect = 1.7
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = +3
Query: 160 NSKNEDQKGCFNCKKPGHFIADCPDL 185
N N Q C NC++ GH DCP L
Sbjct: 285 NHNNPRQTVCMNCQETGHASNDCPSL 362
>AV421607
Length = 245
Score = 30.8 bits (68), Expect = 1.7
Identities = 9/16 (56%), Positives = 12/16 (74%)
Frame = +3
Query: 168 GCFNCKKPGHFIADCP 183
GC+ C +PGH+ DCP
Sbjct: 51 GCYKCGRPGHWSRDCP 98
>TC15740 weakly similar to GB|AAM19935.1|20453393|AY097419 AT5g58640/mzn1_90
{Arabidopsis thaliana;}, partial (65%)
Length = 575
Score = 30.4 bits (67), Expect = 2.3
Identities = 17/44 (38%), Positives = 23/44 (51%), Gaps = 3/44 (6%)
Frame = -3
Query: 552 TKVMVPRQRMFKAHDWRESFVPY--PYNERW-RRSEVWWQPNWK 592
T+V +PR+R K H W+ F Y PYN + R+ Q WK
Sbjct: 534 TQVEIPRRRCNKTHHWKNLFPSYYSPYNTKLNNRNHFA*QTLWK 403
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 30.4 bits (67), Expect = 2.3
Identities = 14/44 (31%), Positives = 21/44 (46%)
Frame = +1
Query: 155 RGSYKNSKNEDQKGCFNCKKPGHFIADCPDLQKEKFKGKSKKSS 198
RG + ED K C+ C +PGHF +C + G ++ S
Sbjct: 127 RGGGRGRGGEDLK-CYECGEPGHFARECRSRGGSRGLGSGRRRS 255
>BP055887
Length = 506
Score = 30.4 bits (67), Expect = 2.3
Identities = 18/41 (43%), Positives = 23/41 (55%)
Frame = +2
Query: 276 KELLSLFEHRTNELTDLKEKYVDLMKQQKSTLLELKASEEE 316
++ LSL TN L KEKY+ + KQQ S + E A E E
Sbjct: 44 RKTLSLKLLNTNNLLWRKEKYIQIEKQQSSNVAEDHAFEGE 166
>TC15523 similar to UP|GRPA_MAIZE (P10979) Glycine-rich RNA-binding,
abscisic acid-inducible protein, complete
Length = 972
Score = 30.0 bits (66), Expect = 3.0
Identities = 10/27 (37%), Positives = 13/27 (48%), Gaps = 1/27 (3%)
Frame = +1
Query: 575 PYNERWRRSEVWWQPNWKD-HWYRYYW 600
P + WRR WW W W R++W
Sbjct: 586 PGSRPWRRRRWWWIRRWSS*RWIRWWW 666
>TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protein, partial
(25%)
Length = 1077
Score = 30.0 bits (66), Expect = 3.0
Identities = 13/33 (39%), Positives = 16/33 (48%), Gaps = 5/33 (15%)
Frame = -1
Query: 573 PYPYNERWR-RSEVWWQPNW----KDHWYRYYW 600
P + E+WR R E WW W WY Y+W
Sbjct: 816 PARWREQWRERREKWWWRRWWWWWS*TWYYYWW 718
>AV780703
Length = 454
Score = 29.6 bits (65), Expect = 3.9
Identities = 14/49 (28%), Positives = 28/49 (56%)
Frame = +3
Query: 101 SKGKISKSSKAYKASESEEESPDGDSDEDQSVKMAMLSNKLEYLARKQK 149
+KG++SK+ E+ ES +GD E V +++ N+L ++ ++ K
Sbjct: 135 TKGRLSKTEPKLGRLENTSESLEGDGRELTGVWLSIKKNRLGFIRKRGK 281
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.356 0.157 0.537
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,382,781
Number of Sequences: 28460
Number of extensions: 323723
Number of successful extensions: 4241
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 3125
Number of HSP's successfully gapped in prelim test: 137
Number of HSP's that attempted gapping in prelim test: 964
Number of HSP's gapped (non-prelim): 3442
length of query: 1595
length of database: 4,897,600
effective HSP length: 103
effective length of query: 1492
effective length of database: 1,966,220
effective search space: 2933600240
effective search space used: 2933600240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC144722.10