BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144721.10 + phase: 0 /pseudo
(813 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780009 172 1e-43
BP066094 98 5e-21
BP075943 94 7e-20
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 84 7e-17
BP084332 83 2e-16
BP057233 80 2e-15
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 77 8e-15
BP043850 72 5e-13
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 70 1e-12
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 70 1e-12
AV424544 70 1e-12
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 62 5e-10
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 41 6e-07
BP084259 49 4e-06
AV766665 49 4e-06
BP037734 33 2e-05
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 45 6e-05
AV411594 44 1e-04
TC18909 40 0.002
TC9992 39 0.003
>AV780009
Length = 529
Score = 172 bits (437), Expect = 1e-43
Identities = 82/169 (48%), Positives = 110/169 (64%)
Frame = -1
Query: 632 ALKRIIRYIQGTKEFGLHLSPSTVTNLSAYSDADWGGCPDTRRSTSGYCVYLGDNLISWS 691
A R++RY++G GL S + L AYSD+DW GCPDTRRS +GY ++LG +LISW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 692 AKRQATLSRSSAEAEYRGVANVVAESCWLRNLLLELHCPISKATVVYCDNVSAIYLSGNP 751
K+Q T+SRSS+EAEYR +A V E WL L L + ++CDN SA++++ NP
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNP 167
Query: 752 VNHQRTKHIEMDIHFVREKVALGQVHVLHVPTRYQYADIFTKGLPKPLF 800
H+RTKHIE+D H VR K+ G +H+L + T +Q ADIFTK P F
Sbjct: 166 TFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKPCPHLCF 20
>BP066094
Length = 532
Score = 98.2 bits (243), Expect = 5e-21
Identities = 48/101 (47%), Positives = 64/101 (62%)
Frame = +2
Query: 438 DRNNPDHVCLLKKSLYGLKQAPRAWYQRFASFLSTIGFVNSKTDHSLFISTHGDDTTYIL 497
D N DH+ LKKSLYGLKQAPRAWY+R +SFL V K D +LF T+ DD +
Sbjct: 227 DEKNSDHIFKLKKSLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQ 406
Query: 498 LYVDDIILTTSSHSLRDTIIAALRSEFSLTDLGPLNYFLGV 538
+YVDDII +++ SL +++EF + +G L YFLG+
Sbjct: 407 IYVDDIIFGSANPSLCKEFSEMMQAEFEMRMMGELKYFLGI 529
>BP075943
Length = 547
Score = 94.4 bits (233), Expect = 7e-20
Identities = 55/164 (33%), Positives = 84/164 (50%), Gaps = 1/164 (0%)
Frame = -3
Query: 494 TYILLYVDDIILTTSSHSLRDTIIAALRSEFSLTDLGPLNYFLGVSATRHSGGLFLHQSK 553
T +LLYVDD++L + + ++L +F + DLG YFLG+ R + G+ L+Q K
Sbjct: 527 TALLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRK 348
Query: 554 YAEDIISRAKMVNCKPALTPVDTKSK-LSATSGEKFDDSTLYRSLAGALQYLTFTRPDIS 612
YA +IS + +P T S+ L +G D YR + G L YL TRPDI+
Sbjct: 347 YALQLISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDIT 168
Query: 613 YAVQQICLYMHDPRTSHFAALKRIIRYIQGTKEFGLHLSPSTVT 656
+AV Q+ ++ P H L ++Y+ G+ GL S+ T
Sbjct: 167 FAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASSST 36
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 84.3 bits (207), Expect = 7e-17
Identities = 39/91 (42%), Positives = 57/91 (61%)
Frame = +1
Query: 719 WLRNLLLELHCPISKATVVYCDNVSAIYLSGNPVNHQRTKHIEMDIHFVREKVALGQVHV 778
WL LL +L P + +VYCDN SA +++ NPV H+RTKHIE+D H VRE++ G +H+
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 779 LHVPTRYQYADIFTKGLPKPLFDEFRSSLSI 809
L + + ADI+TK L F + + L +
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>BP084332
Length = 368
Score = 83.2 bits (204), Expect = 2e-16
Identities = 46/117 (39%), Positives = 69/117 (58%), Gaps = 1/117 (0%)
Frame = +2
Query: 686 NLISWSAK-RQATLSRSSAEAEYRGVANVVAESCWLRNLLLELHCPISKATVVYCDNVSA 744
N+ W A Q+T++ S+AEAEY A + W+++ L + + +YCDN +A
Sbjct: 2 NVNFWEAT*GQSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQI-LESNIPIYCDNTAA 178
Query: 745 IYLSGNPVNHQRTKHIEMDIHFVREKVALGQVHVLHVPTRYQYADIFTKGLPKPLFD 801
I LS NP+ H R KHIE+ HF+R+ V G + + V T +Q+ADIFTK L + F+
Sbjct: 179 ISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFN 349
>BP057233
Length = 473
Score = 79.7 bits (195), Expect = 2e-15
Identities = 38/85 (44%), Positives = 55/85 (64%), Gaps = 1/85 (1%)
Frame = -2
Query: 730 PISKATVVYCDNVSAIYLSGNPVNHQRTKHIEMDIHFVREKVALGQVHVLHVPTRYQYAD 789
P+ + +++CDN+SA L+ NPV H R+KHIE+D+H++R++V +V V +VPT Q AD
Sbjct: 466 PMPRKPILWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIAD 287
Query: 790 IFTKGLPKPLFDEFRSSLS-IHPPP 813
TK L F + R L IH PP
Sbjct: 286 CLTKPLSHTRFSQLRDKLGVIHSPP 212
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 77.4 bits (189), Expect = 8e-15
Identities = 40/94 (42%), Positives = 58/94 (61%)
Frame = +3
Query: 716 ESCWLRNLLLELHCPISKATVVYCDNVSAIYLSGNPVNHQRTKHIEMDIHFVREKVALGQ 775
E+ WL L +L V+YCDN SA++L+ N V H+RT++IE+D H V KV G
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 776 VHVLHVPTRYQYADIFTKGLPKPLFDEFRSSLSI 809
+H+LHVP+ Q AD+FTK + +F ++SI
Sbjct: 201 LHLLHVPSSDQVADVFTKTI-----SQFTGAISI 287
>BP043850
Length = 515
Score = 71.6 bits (174), Expect = 5e-13
Identities = 41/99 (41%), Positives = 55/99 (55%)
Frame = -1
Query: 715 AESCWLRNLLLELHCPISKATVVYCDNVSAIYLSGNPVNHQRTKHIEMDIHFVREKVALG 774
+E WLR LL EL S+ T ++ DN SAI ++ NPV H+ T+HIE+D H VRE
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 775 QVHVLHVPTRYQYADIFTKGLPKPLFDEFRSSLSIHPPP 813
+ + HV T Q ADI TK L + + S L + P
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKLMLVESP 219
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 70.5 bits (171), Expect = 1e-12
Identities = 45/147 (30%), Positives = 72/147 (48%), Gaps = 9/147 (6%)
Frame = +2
Query: 466 FASFLSTIGFVNSKTDHSLFISTHGD-DTTYILLYVDDIILTTSSHSLRDTIIAALRSEF 524
F SF+ ++G+ +DH + D D +LLYVDD+++ + + A L EF
Sbjct: 2 FDSFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREF 181
Query: 525 SLTDLGPLNYFLG--VSATRHSGGLFLHQSKYAEDIISRAKMVNCKPALTPVDTKSKLS- 581
+ DLGP N LG + R ++L Q Y + ++ R M +C P TP+ KLS
Sbjct: 182 DMKDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSS 361
Query: 582 ----ATSGEKFDDSTL-YRSLAGALQY 603
++ E+ + S + Y S G+L Y
Sbjct: 362 SMIPSSEAERMEMSRVPYASAVGSLMY 442
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 70.5 bits (171), Expect = 1e-12
Identities = 35/91 (38%), Positives = 53/91 (57%)
Frame = +1
Query: 719 WLRNLLLELHCPISKATVVYCDNVSAIYLSGNPVNHQRTKHIEMDIHFVREKVALGQVHV 778
WL +L +L P +++YCD+ SA +++ N V H+RTKH+++D H VREK+ H+
Sbjct: 13 WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHL 192
Query: 779 LHVPTRYQYADIFTKGLPKPLFDEFRSSLSI 809
L + + Q ADI TK L F S L +
Sbjct: 193 LPISSVDQTADILTKPLESGPFSHLVSKLGV 285
>AV424544
Length = 276
Score = 70.5 bits (171), Expect = 1e-12
Identities = 37/90 (41%), Positives = 51/90 (56%)
Frame = +3
Query: 465 RFASFLSTIGFVNSKTDHSLFISTHGDDTTYILLYVDDIILTTSSHSLRDTIIAALRSEF 524
+ +S+L +G++ S DHSLF T IL+YVDD+IL + + + L +F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 525 SLTDLGPLNYFLGVSATRHSGGLFLHQSKY 554
+ DLG L YFLG+ R S GLFL Q KY
Sbjct: 186 RIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 61.6 bits (148), Expect = 5e-10
Identities = 48/144 (33%), Positives = 70/144 (48%), Gaps = 2/144 (1%)
Frame = -1
Query: 551 QSKYAEDIISRAKMVNCKPALTPVDTKS-KLSATSGEKFDDSTLYRSLAGALQYLTFTRP 609
Q Y +DI+ + KM N K T + K + +G K DST Y+SL G+++YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 610 DISYAVQQICLYMHDPRTSHFAALKRIIRYIQGTKEFGLHLSPSTVTNLSAYSDADWGGC 669
DI V +M +P H +R +RYI+GT + G+ + T+ N
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGIFMLVITM*N-----------S 416
Query: 670 PDTRRST-SGYCVYLGDNLISWSA 692
DT+ T GY +LG ISWS+
Sbjct: 415 LDTQIVTRPGYAFHLGRGAISWSS 344
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 41.2 bits (95), Expect(2) = 6e-07
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = +2
Query: 735 TVVYCDNVSAIYLSGNPVNHQRTKHIEMDIHF 766
TV+YCDN AIYL N H R+KHI++ H+
Sbjct: 167 TVLYCDNQGAIYLGQNSTFHSRSKHIDVRYHW 262
Score = 29.6 bits (65), Expect(2) = 6e-07
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = +3
Query: 688 ISWSAKRQATLSRSSAEAEYRGVANVVAESCWLRNLL 724
++W ++ Q ++ S+AEAE+ E W++N L
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFL 137
>BP084259
Length = 385
Score = 48.5 bits (114), Expect = 4e-06
Identities = 21/59 (35%), Positives = 33/59 (55%)
Frame = -3
Query: 708 RGVANVVAESCWLRNLLLELHCPISKATVVYCDNVSAIYLSGNPVNHQRTKHIEMDIHF 766
R + + AE WL+ LL EL + K ++ +N++A Y+ N V H KH+ +D HF
Sbjct: 179 RTIGSTAAEFLWLQQLLKELQFSLPKVPCIFSNNINATYVCLNLVFHSHMKHVALDCHF 3
>AV766665
Length = 601
Score = 48.5 bits (114), Expect = 4e-06
Identities = 25/74 (33%), Positives = 41/74 (54%)
Frame = +3
Query: 635 RIIRYIQGTKEFGLHLSPSTVTNLSAYSDADWGGCPDTRRSTSGYCVYLGDNLISWSAKR 694
RI Y++ G +++ ++ AD+ G R ST GY ++L NL++W +K+
Sbjct: 372 RIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTWRSKQ 551
Query: 695 QATLSRSSAEAEYR 708
Q ++RSS EAE R
Sbjct: 552 QNIIARSSGEAELR 593
>BP037734
Length = 490
Score = 33.1 bits (74), Expect(2) = 2e-05
Identities = 16/37 (43%), Positives = 22/37 (59%)
Frame = +3
Query: 512 LRDTIIAALRSEFSLTDLGPLNYFLGVSATRHSGGLF 548
+RD + +L +EF + DLGPL FL + A G LF
Sbjct: 363 MRDRLKNSLTAEFKIEDLGPLTCFLEIIARSRKGLLF 473
Score = 32.3 bits (72), Expect(2) = 2e-05
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +2
Query: 452 LYGLKQAPRAWYQRFASFLSTIGFVNSKTDHSL 484
LYGLKQ PR W++RF F+ SK H++
Sbjct: 194 LYGLKQLPRDWFERFT-------FLRSKAVHNV 271
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein,
partial (4%)
Length = 528
Score = 44.7 bits (104), Expect = 6e-05
Identities = 22/59 (37%), Positives = 34/59 (57%)
Frame = +3
Query: 751 PVNHQRTKHIEMDIHFVREKVALGQVHVLHVPTRYQYADIFTKGLPKPLFDEFRSSLSI 809
P++ T +IE HF+R++V G++ + H T Q ADI TKGL F R+ L++
Sbjct: 45 PMDVVNTLNIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 33.1 bits (74), Expect = 0.18
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = +2
Query: 740 DNVSAIYLSGNPVNHQRTKHIE 761
DN SAI L+ NPV+H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>AV411594
Length = 244
Score = 43.5 bits (101), Expect = 1e-04
Identities = 22/65 (33%), Positives = 32/65 (48%)
Frame = +2
Query: 535 FLGVSATRHSGGLFLHQSKYAEDIISRAKMVNCKPALTPVDTKSKLSATSGEKFDDSTLY 594
FL + G+ L + KYA ++ + + CKP P+D KL++T GE D TLY
Sbjct: 50 FLRT*IAKSKKGILLSRRKYALQLLDDTRNMGCKPPPFPLDPSIKLNSTDGELLSDVTLY 229
Query: 595 RSLAG 599
G
Sbjct: 230 IRFIG 244
>TC18909
Length = 621
Score = 39.7 bits (91), Expect = 0.002
Identities = 24/71 (33%), Positives = 35/71 (48%), Gaps = 1/71 (1%)
Frame = +2
Query: 607 TRPDISYAVQQICLYMHDPRTSHFAALKRIIRYI-QGTKEFGLHLSPSTVTNLSAYSDAD 665
T P IS++V ++C ++ + H A K I+ + G + TN A+SDAD
Sbjct: 326 TSPQISFSVNKVC*FLSESLEEHGTAGKCILNVT*KAL*TMGFFIQ----TNFPAFSDAD 493
Query: 666 WGGCPDTRRST 676
W D RRST
Sbjct: 494 WASNVDDRRST 526
>TC9992
Length = 508
Score = 38.9 bits (89), Expect = 0.003
Identities = 28/101 (27%), Positives = 50/101 (48%), Gaps = 4/101 (3%)
Frame = +3
Query: 711 ANVVAESCWLRNLLLELHCPISKATVVYCDNVSAIYLSGNPVNHQRTKHIEMDIHFVRE- 769
A VV W++ LLL+L + + DN + I +S +P+ ++T ++ +F+RE
Sbjct: 60 ATVVNPPLWIQKLLLDLPMEPMEHPKFFVDN*AIISISAHPLFQRKTHPSKIQFYFLREA 239
Query: 770 ---KVALGQVHVLHVPTRYQYADIFTKGLPKPLFDEFRSSL 807
+ + +LH R Q+ ++F + LP F F SL
Sbjct: 240 EW*EFRRSNLRILH-S*RAQWHEVFIQALPPSEF*VFS*SL 359
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.333 0.143 0.459
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,971,024
Number of Sequences: 28460
Number of extensions: 297982
Number of successful extensions: 2470
Number of sequences better than 10.0: 63
Number of HSP's better than 10.0 without gapping: 2409
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2460
length of query: 813
length of database: 4,897,600
effective HSP length: 98
effective length of query: 715
effective length of database: 2,108,520
effective search space: 1507591800
effective search space used: 1507591800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 59 (27.3 bits)
Medicago: description of AC144721.10