BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144720.7 + phase: 2 /pseudo/partial
(1007 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU089540 285 3e-77
AW720269 114 8e-26
AV771159 87 2e-25
AV416268 93 2e-19
BP055095 85 7e-17
BP029778 82 6e-16
BP057421 72 6e-13
TC18647 similar to UP|Q9ASR9 (Q9ASR9) At2g01320/F10A8.20, partia... 68 6e-12
BP066380 39 3e-08
BP055390 50 1e-06
BG662257 46 3e-05
TC11425 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proeti... 44 1e-04
CB829290 43 3e-04
AV406931 43 3e-04
TC15722 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transpor... 42 4e-04
TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide... 42 5e-04
AV413975 40 0.002
CB827630 40 0.002
TC9119 homologue to AAQ65167 (AAQ65167) At1g64550, partial (20%) 39 0.003
TC10604 similar to AAQ89671 (AAQ89671) At4g33460, partial (36%) 37 0.020
>AU089540
Length = 660
Score = 285 bits (729), Expect = 3e-77
Identities = 140/217 (64%), Positives = 175/217 (80%), Gaps = 3/217 (1%)
Frame = +1
Query: 688 PIPLDMRQHAAQFGIPQSVNSANDL---GEVGKTFAGELWNDVRSNVELRGEKIRLNFLK 744
P+P DM +A Q S + ND + ++FAGE W D++SNV+ + I FLK
Sbjct: 10 PVPPDMLHYADQIAAASSSSHTNDAIKGPDEEQSFAGEFWEDMKSNVQNHKDHIEATFLK 189
Query: 745 SKDLSNRKTPGVFKQYKYFLIRVGKQRLREARIQAVDYLILLLAGACLGSITKSSDQTFG 804
+KDLSNR+TPGV +QY+Y++ R+ KQ+LRE + QAVDYL+LL+AGA LG++TK +D+TFG
Sbjct: 190 TKDLSNRRTPGVARQYRYYVGRISKQQLREGKSQAVDYLLLLVAGAILGTLTKVNDETFG 369
Query: 805 ASGYTYTVIAVSLLCKIAALRSFSLDKLHYWRESDSGMSSLAYFLSKDTMDHFNTVIKPV 864
+ GYTYTVIAVSLLCKIAALRSFSLDKL YWRES SG+SSLA+FLSKDT+D FNT+IKPV
Sbjct: 370 SLGYTYTVIAVSLLCKIAALRSFSLDKLQYWRESASGISSLAHFLSKDTVDLFNTIIKPV 549
Query: 865 VYLSMFYFLTNPRSTFTDNYIVLLCLVYCVTGIAYAL 901
VYLSMFYF +NPRS+F NY+VL+CLVYCVTGIAYA+
Sbjct: 550 VYLSMFYFFSNPRSSFGSNYMVLVCLVYCVTGIAYAI 660
>AW720269
Length = 524
Score = 114 bits (285), Expect = 8e-26
Identities = 70/167 (41%), Positives = 106/167 (62%), Gaps = 2/167 (1%)
Frame = +3
Query: 413 LTLKAQNKHILRNVTGKIKPGRITAIMGPSGAGKTTFLSALAGKALGC-LVTGSILINGR 471
LT K + +IL++V+ + I A++GPSG GK+T L +AG+ +I IN
Sbjct: 27 LTQKPKPVNILKSVSFVARSSEIVAVVGPSGTGKSTLLRIIAGRVKDRDFDPKTISINDH 206
Query: 472 N-ESIHSFKKIIGFVPQDDVVHGNLTVEENLWFSAQCRLSADLSKPEKVLVVERVIEFLG 530
S +KI GFV Q+D + LTV+E L FSA+ RL +++ ++ + VE +++ LG
Sbjct: 207 PMTSPAQLRKICGFVAQEDNLLPLLTVKETLLFSAKFRLK-EMTPNDREMRVESLMQELG 383
Query: 531 LQSVRNSVVGTVEKRGVSGGQRKRVNVGLEMVMEPSLLMLDEPTSGL 577
L V +S VG E RG+SGG+RKRV++G++M+ P +L+LDEPTSGL
Sbjct: 384 LFHVSDSFVGDEENRGISGGERKRVSIGVDMIHNPPILLLDEPTSGL 524
>AV771159
Length = 486
Score = 87.0 bits (214), Expect(2) = 2e-25
Identities = 42/103 (40%), Positives = 65/103 (62%)
Frame = -3
Query: 560 EMVMEPSLLMLDEPTSGLDSASSQLLLRALRREALEGVNICMVVHQPSYALFNMFDDLIL 619
++++ PSLL+LDEPTSGLDS ++ +L + R A G + +HQPS L+ MFD ++L
Sbjct: 343 KLLIHPSLLLLDEPTSGLDSTTALRILNTIPRLATGGRTVVTTIHQPSSRLYYMFDKVVL 164
Query: 620 LGKGGLMVYHGSAKKVEEYFSGLGINVPERINPPDYYIDILEG 662
L + G +Y+G A EYFS +G + +NP D +D+ +G
Sbjct: 163 LSE-GCPIYYGPASTALEYFSSVGFSTCVTVNPADLLLDLADG 38
Score = 46.6 bits (109), Expect(2) = 2e-25
Identities = 23/48 (47%), Positives = 34/48 (69%)
Frame = -2
Query: 513 LSKPEKVLVVERVIEFLGLQSVRNSVVGTVEKRGVSGGQRKRVNVGLE 560
L++ EKV VERVI LGL R+S++G RG+SG +++RV++G E
Sbjct: 485 LTRDEKVQHVERVITELGLSGCRSSMIGGPLLRGISGAEKRRVSIGQE 342
>AV416268
Length = 424
Score = 93.2 bits (230), Expect = 2e-19
Identities = 54/132 (40%), Positives = 81/132 (60%), Gaps = 3/132 (2%)
Frame = +3
Query: 495 LTVEENLWFSAQCRLSADLSKPEKVLVVERVIEFLGLQSVRNSVVGTVEKRGVSGGQRKR 554
LTV E +++SA +L +SK EK + I+ +GLQ N+ +G +GVSGGQ++R
Sbjct: 12 LTVGEAVYYSAHLQLPDSMSKSEKRERADFTIKEMGLQDAINTRIGGWGSKGVSGGQKRR 191
Query: 555 VNVGLEMVMEPSLLMLDEPTSGLDSASS-QLLLRALRREALEGVNICMV--VHQPSYALF 611
V++ +E++ P LL LDEPTSGLDSA+S ++ R +G+ +V +HQPS +F
Sbjct: 192 VSICIEILTHPRLLFLDEPTSGLDSAASYHVISRISSLNKKDGIQRTIVASIHQPSNEIF 371
Query: 612 NMFDDLILLGKG 623
+F L LL G
Sbjct: 372 QLFHSLCLLSSG 407
>BP055095
Length = 538
Score = 84.7 bits (208), Expect = 7e-17
Identities = 48/142 (33%), Positives = 84/142 (58%), Gaps = 2/142 (1%)
Frame = +3
Query: 520 LVVERVIEFLGLQSVRNSVVGTVEKRGVSGGQRKRVNVGLEMVMEPSLLMLDEPTSGLDS 579
LV + ++ LGL + ++G +RG+SGGQ+KRV G +V L +DE ++GLDS
Sbjct: 21 LVTDYALKVLGLDICADVMIGDEMRRGISGGQKKRVTTGEMLVGPAKALFMDEISTGLDS 200
Query: 580 ASSQLLLRALRREALEGVNICMVVH--QPSYALFNMFDDLILLGKGGLMVYHGSAKKVEE 637
+++ + + + R+ + +++ MV+ QP+ F +FDD+ILL +G + VY G + V E
Sbjct: 201 STTFQICKFM-RQMVHIMDVTMVISLLQPAPETFELFDDIILLSEGQI-VYQGPRENVLE 374
Query: 638 YFSGLGINVPERINPPDYYIDI 659
+F +G PER D+ ++
Sbjct: 375 FFEYMGFKCPERKGAADFLQEV 440
>BP029778
Length = 438
Score = 81.6 bits (200), Expect = 6e-16
Identities = 41/88 (46%), Positives = 58/88 (65%)
Frame = -2
Query: 422 ILRNVTGKIKPGRITAIMGPSGAGKTTFLSALAGKALGCLVTGSILINGRNESIHSFKKI 481
+L NV+G PG +TA+MG SGAGKTT + LAG+ G + G I I+G + H+F ++
Sbjct: 278 LLSNVSGVFAPGVLTALMGSSGAGKTTLMDVLAGRKTGGYIEGDIKISGYPKVQHTFARV 99
Query: 482 IGFVPQDDVVHGNLTVEENLWFSAQCRL 509
G+V Q+D+ +TVEE+L FSA RL
Sbjct: 98 AGYVEQNDIHSPQVTVEESLLFSALLRL 15
>BP057421
Length = 547
Score = 71.6 bits (174), Expect = 6e-13
Identities = 49/162 (30%), Positives = 82/162 (50%), Gaps = 32/162 (19%)
Frame = +3
Query: 462 VTGSILINGRNESIHSFKKIIGFVPQDDVVHGNLTVEENLWFSAQCR------------- 508
VTG I NG + +K ++ Q+DV G +TV+E L FSA+C+
Sbjct: 45 VTGEITYNGHKLNEFVPRKTAAYISQNDVHVGEMTVKETLDFSARCQGVGTRYDXLSELG 224
Query: 509 ------------------LSADLSKPEKVLVVERVIEFLGLQSVRNSVVGTVEKRGVSGG 550
+ L E L+ + ++ LGL ++++VG RGVSGG
Sbjct: 225 RREKEAGIFPEAELDLFMKATALKGTESSLITDYTLKILGLDICKDTIVGDEMHRGVSGG 404
Query: 551 QRKRVNVGLEMVMEPS-LLMLDEPTSGLDSASSQLLLRALRR 591
Q+KRV G EM++ P+ L +DE ++GLDS+++ +++ L++
Sbjct: 405 QKKRVTTG-EMIVGPTKTLFMDEISTGLDSSTTFQIVKCLQQ 527
>TC18647 similar to UP|Q9ASR9 (Q9ASR9) At2g01320/F10A8.20, partial (17%)
Length = 430
Score = 68.2 bits (165), Expect = 6e-12
Identities = 39/102 (38%), Positives = 62/102 (60%), Gaps = 3/102 (2%)
Frame = +1
Query: 420 KHILRNVTGKIKPGRITAIMGPSGAGKTTFLSALAGKALG---CLVTGSILINGRNESIH 476
+ +L+NV+G+ KPGR+ AIMGPSG+GKTT L+ LAG+ ++G + NG+ S +
Sbjct: 100 RFLLKNVSGEAKPGRLLAIMGPSGSGKTTLLNVLAGQLAASPRLHLSGLLEFNGKPGSKN 279
Query: 477 SFKKIIGFVPQDDVVHGNLTVEENLWFSAQCRLSADLSKPEK 518
++K +V Q+D+ LTV E L + + +L S E+
Sbjct: 280 AYK--FAYVRQEDLFFSQLTVRETLSLATELQLPNISSAEER 399
>BP066380
Length = 385
Score = 39.3 bits (90), Expect(2) = 3e-08
Identities = 16/26 (61%), Positives = 21/26 (80%)
Frame = -2
Query: 951 VIANAERYQGVWLITRCGSLLKSGYN 976
VI+ A+RY GVWLI+RCG+L GY+
Sbjct: 384 VISKAKRYSGVWLISRCGALNSYGYD 307
Score = 36.2 bits (82), Expect(2) = 3e-08
Identities = 14/28 (50%), Positives = 21/28 (75%)
Frame = -3
Query: 980 WSLCISILILMGVIGRAIAFFCMVTFKK 1007
++ C+ +L++ G I R AFFCMVTF+K
Sbjct: 296 YNYCLLLLVVTGAISRMRAFFCMVTFQK 213
>BP055390
Length = 488
Score = 50.4 bits (119), Expect = 1e-06
Identities = 42/144 (29%), Positives = 74/144 (51%), Gaps = 12/144 (8%)
Frame = +3
Query: 500 NLWFSAQCR--------LSADLSKPEKVLVVERVIEFLGLQSVR--NSVVGTVEKRGV-- 547
N+WF ++ R L L K V ++ L SV ++++ V +RGV
Sbjct: 54 NIWFLSKNRWAKI*ISSLYTKLGKRITVKCALCILVMLTKDSVN*CSNLLTQVGQRGVQL 233
Query: 548 SGGQRKRVNVGLEMVMEPSLLMLDEPTSGLDSASSQLLLRALRREALEGVNICMVVHQPS 607
SGGQ++R+ + ++ P +L+LDE TS LD+ S +L+ AL A+ G + ++ H+ S
Sbjct: 234 SGGQKQRIAIARAILKNPPILLLDEATSALDTESEKLVQEAL-ETAMHGRTVILIAHRLS 410
Query: 608 YALFNMFDDLILLGKGGLMVYHGS 631
+ D+I + + G +V G+
Sbjct: 411 TV---VNADVIAVVENGQVVETGT 473
>BG662257
Length = 336
Score = 46.2 bits (108), Expect = 3e-05
Identities = 31/79 (39%), Positives = 48/79 (60%), Gaps = 2/79 (2%)
Frame = +1
Query: 531 LQSVRNSVVGTVEKRGV--SGGQRKRVNVGLEMVMEPSLLMLDEPTSGLDSASSQLLLRA 588
LQ ++VVG RG+ SGGQ++RV + +V P +L+LDE TS LD+ S +++ A
Sbjct: 94 LQQGYDTVVGD---RGIQLSGGQKQRVAIARAIVKSPKILLLDEATSALDAESEKVVQDA 264
Query: 589 LRREALEGVNICMVVHQPS 607
L R ++ I +V H+ S
Sbjct: 265 LDRVRVDRTTI-VVAHRLS 318
>TC11425 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proetin, partial
(9%)
Length = 712
Score = 44.3 bits (103), Expect = 1e-04
Identities = 31/91 (34%), Positives = 55/91 (60%), Gaps = 2/91 (2%)
Frame = +2
Query: 542 VEKRG--VSGGQRKRVNVGLEMVMEPSLLMLDEPTSGLDSASSQLLLRALRREALEGVNI 599
V +RG +SGGQ++RV + ++ P++L+LDE TS LD+ S +++ AL + + +
Sbjct: 86 VGERGTLLSGGQKQRVAIARAIIKTPNILLLDEATSALDAESERVVQDALDKVMVNRTTV 265
Query: 600 CMVVHQPSYALFNMFDDLILLGKGGLMVYHG 630
+V H+ S + N D+I + K G++V G
Sbjct: 266 -IVAHRLS-TIKNA--DVITVLKNGVIVEKG 346
>CB829290
Length = 546
Score = 42.7 bits (99), Expect = 3e-04
Identities = 32/110 (29%), Positives = 59/110 (53%), Gaps = 2/110 (1%)
Frame = -3
Query: 541 TVEKRGV--SGGQRKRVNVGLEMVMEPSLLMLDEPTSGLDSASSQLLLRALRREALEGVN 598
TV + G S GQR+ V +G ++ + +L+LDE T+ +D+A+ L+ + LR+ E
Sbjct: 523 TVSENGENWSMGQRQLVCLGRVLLKKSKVLVLDEATASVDTATDNLIQQTLRQHFSES-T 347
Query: 599 ICMVVHQPSYALFNMFDDLILLGKGGLMVYHGSAKKVEEYFSGLGINVPE 648
+ + H+ + + + D ++LL +G + Y AK +E+ S V E
Sbjct: 346 VITIAHRITSVIDS--DMVLLLSQGLIEEYDSPAKLLEDKSSSFAQLVAE 203
>AV406931
Length = 438
Score = 42.7 bits (99), Expect = 3e-04
Identities = 19/55 (34%), Positives = 33/55 (59%)
Frame = +3
Query: 605 QPSYALFNMFDDLILLGKGGLMVYHGSAKKVEEYFSGLGINVPERINPPDYYIDI 659
QP+ +++FDD+IL+ G + VYHG + V ++F +G PER D+ ++
Sbjct: 69 QPAPETYDLFDDIILISDGQV-VYHGPREYVLDFFESMGFKCPERKGAADFLQEV 230
>TC15722 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transporter,
partial (37%)
Length = 624
Score = 42.4 bits (98), Expect = 4e-04
Identities = 25/76 (32%), Positives = 47/76 (60%), Gaps = 5/76 (6%)
Frame = +3
Query: 522 VERVIEFLGLQSVRNSVVGTVEK--RGVSGGQRKRVNVGLEMVMEPSLLMLDEPTSGLD- 578
++R I ++GL + +++K + +SGG ++R+ + +++V P LL+LDEP +GLD
Sbjct: 6 LQRAINWVGLSGI------SLDKNPQSLSGGYKRRLALAIQLVQVPDLLILDEPLAGLDW 167
Query: 579 --SASSQLLLRALRRE 592
A LL+ L++E
Sbjct: 168 KARADVVKLLKHLKKE 215
>TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide
transporter 2 (APT2) (Peptide transporter TAP2) (Peptide
transporter PSF2) (Peptide supply factor 2) (PSF-2)
(Peptide transporter involved in antigen processing 2),
partial (4%)
Length = 530
Score = 42.0 bits (97), Expect = 5e-04
Identities = 22/77 (28%), Positives = 41/77 (52%)
Frame = +1
Query: 396 TEKSKRPFIEISFRDLTLTLKAQNKHILRNVTGKIKPGRITAIMGPSGAGKTTFLSALAG 455
T+ S+ P ++++ DL + ++K +L+ + I G + A+MG +G+GK+TF L G
Sbjct: 139 TDTSQSPLLQVN--DLKAKIVGEHKDLLKGLNLTINRGEVHALMGANGSGKSTFAKVLVG 312
Query: 456 KALGCLVTGSILINGRN 472
+ G + G N
Sbjct: 313 HPDYEVTGGRAVYKGEN 363
>AV413975
Length = 343
Score = 40.0 bits (92), Expect = 0.002
Identities = 22/67 (32%), Positives = 37/67 (54%), Gaps = 1/67 (1%)
Frame = +2
Query: 405 EISFRDLTLTLKAQNKH-ILRNVTGKIKPGRITAIMGPSGAGKTTFLSALAGKALGCLVT 463
E+ D+ + ++ H +L+ +T K+ PG A++GPSG GKTT + + +
Sbjct: 146 EVELDDVWFSYPSRPSHPVLKGITMKLHPGSKVALVGPSGGGKTTIANLI--ERFYDPTK 319
Query: 464 GSILING 470
G IL+NG
Sbjct: 320 GKILLNG 340
>CB827630
Length = 405
Score = 39.7 bits (91), Expect = 0.002
Identities = 23/75 (30%), Positives = 45/75 (59%), Gaps = 2/75 (2%)
Frame = +2
Query: 518 KVLVVERVIEFLGLQSVRNSVVGTVEKRG--VSGGQRKRVNVGLEMVMEPSLLMLDEPTS 575
K LV+ + +E L+S+ G +K+ SGG ++R+ V + ++ +P ++ +DEP++
Sbjct: 47 KGLVLTQAVEE-SLKSLNLFHGGVADKQAGKYSGGMKRRLIVAISLIGDPRVVYMDEPST 223
Query: 576 GLDSASSQLLLRALR 590
GLD AS + L ++
Sbjct: 224 GLDPASRKSLWNVVK 268
>TC9119 homologue to AAQ65167 (AAQ65167) At1g64550, partial (20%)
Length = 666
Score = 39.3 bits (90), Expect = 0.003
Identities = 25/85 (29%), Positives = 44/85 (51%)
Frame = +3
Query: 547 VSGGQRKRVNVGLEMVMEPSLLMLDEPTSGLDSASSQLLLRALRREALEGVNICMVVHQP 606
+SGGQ+ RV +P +++LDEP+ LD + + L++ L + I MV H
Sbjct: 159 LSGGQKSRVAFAKITFKKPHIILLDEPSHHLDLDAVEALIQGL---VMFQGGILMVSHD- 326
Query: 607 SYALFNMFDDLILLGKGGLMVYHGS 631
+ + D+L ++ +G +HGS
Sbjct: 327 EHLISGSVDELWVVSQGTATPFHGS 401
>TC10604 similar to AAQ89671 (AAQ89671) At4g33460, partial (36%)
Length = 498
Score = 36.6 bits (83), Expect = 0.020
Identities = 25/82 (30%), Positives = 45/82 (54%), Gaps = 4/82 (4%)
Frame = +2
Query: 392 MATNTEKSKR-PFIEISFRDLTLTLKAQNKH---ILRNVTGKIKPGRITAIMGPSGAGKT 447
+ TNT +S + I R+L + A+ + +LR+ + +I G+ ++GP+G GK+
Sbjct: 98 LPTNTIRSNSVENVAIVGRNLNFSFTARQTNDVRVLRDCSLRIPSGQFWMLLGPNGCGKS 277
Query: 448 TFLSALAGKALGCLVTGSILIN 469
T L LAG L +G++ +N
Sbjct: 278 TLLKILAG--LLAPTSGTVYVN 337
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.138 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,945,811
Number of Sequences: 28460
Number of extensions: 262328
Number of successful extensions: 1702
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 1679
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1696
length of query: 1007
length of database: 4,897,600
effective HSP length: 99
effective length of query: 908
effective length of database: 2,080,060
effective search space: 1888694480
effective search space used: 1888694480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144720.7