BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144720.4 + phase: 0
(83 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14282 75 2e-15
TC14364 weakly similar to UP|Q9SVZ7 (Q9SVZ7) Pumilio-like protei... 74 5e-15
TC10705 44 4e-06
TC10553 44 5e-06
TC20139 similar to GB|AAG31805.1|11139702|AF315590 Pumilio 2 {Mu... 30 0.078
TC10753 29 0.17
AV409964 25 2.5
TC14476 homologue to UP|TBB3_WHEAT (Q9ZRB0) Tubulin beta-3 chain... 25 3.3
TC7866 homologue to UP|SAHH_CATRO (P35007) Adenosylhomocysteinas... 25 3.3
TC15780 similar to UP|Q8VAD1 (Q8VAD1) Wsv494 (WSSV020), partial ... 24 4.3
BP060017 23 7.3
TC17484 weakly similar to UP|DBAT_TAXCU (Q9M6E2) 10-deacetylbacc... 23 7.3
TC16048 similar to UP|BAD08735 (BAD08735) 1-phosphatidylinositol... 23 7.3
BP047482 23 9.6
TC14805 similar to UP|RP3A_ARATH (Q39211) DNA-directed RNA polym... 23 9.6
TC14541 weakly similar to UP|Q8LPS4 (Q8LPS4) At3g06670/T8E24.10,... 23 9.6
BP049568 23 9.6
TC12625 similar to UP|Q84TG1 (Q84TG1) At3g09720, partial (34%) 23 9.6
>TC14282
Length = 647
Score = 75.5 bits (184), Expect = 2e-15
Identities = 35/43 (81%), Positives = 38/43 (87%)
Frame = +2
Query: 18 LETCDDQSLELILSRIKVHLNALKRYTYGKHIVSRVEKLITTG 60
LETCDD ELILSRIKVHLNALK+YTYGKHIV+RVEKL+ G
Sbjct: 2 LETCDDHQRELILSRIKVHLNALKKYTYGKHIVARVEKLVAAG 130
>TC14364 weakly similar to UP|Q9SVZ7 (Q9SVZ7) Pumilio-like protein, partial
(8%)
Length = 702
Score = 73.9 bits (180), Expect = 5e-15
Identities = 32/52 (61%), Positives = 41/52 (78%)
Frame = +3
Query: 4 MMKDPFGNYVVQKVLETCDDQSLELILSRIKVHLNALKRYTYGKHIVSRVEK 55
MMKDPF NYVVQKV++ C + +LS I+VH ALK+YTYGKHIV+R+E+
Sbjct: 54 MMKDPFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYGKHIVARLEQ 209
>TC10705
Length = 503
Score = 44.3 bits (103), Expect = 4e-06
Identities = 19/30 (63%), Positives = 26/30 (86%)
Frame = +3
Query: 28 LILSRIKVHLNALKRYTYGKHIVSRVEKLI 57
L+LSRI+ H +ALK+Y YGKHIV+R E+L+
Sbjct: 12 LLLSRIRAHPHALKKYPYGKHIVARFEQLL 101
>TC10553
Length = 694
Score = 43.9 bits (102), Expect = 5e-06
Identities = 18/33 (54%), Positives = 27/33 (81%)
Frame = +3
Query: 28 LILSRIKVHLNALKRYTYGKHIVSRVEKLITTG 60
L+L RI+ H +ALK+YT+GKH+V+R E+L+ G
Sbjct: 36 LLLFRIRAHTHALKKYTFGKHLVARFEQLLGEG 134
>TC20139 similar to GB|AAG31805.1|11139702|AF315590 Pumilio 2 {Mus
musculus;} , partial (7%)
Length = 425
Score = 30.0 bits (66), Expect = 0.078
Identities = 16/58 (27%), Positives = 29/58 (49%)
Frame = +2
Query: 4 MMKDPFGNYVVQKVLETCDDQSLELILSRIKVHLNALKRYTYGKHIVSRVEKLITTGG 61
+ +D +GNYVVQ VLE I+ + ++ + + + ++ VEK + GG
Sbjct: 158 LAQDQYGNYVVQHVLEHGKPHERSAIIKELTGNIVQMSQQKFASNV---VEKCLAFGG 322
Score = 30.0 bits (66), Expect = 0.078
Identities = 15/47 (31%), Positives = 27/47 (56%), Gaps = 1/47 (2%)
Frame = +2
Query: 8 PFGNYVVQKVLETC-DDQSLELILSRIKVHLNALKRYTYGKHIVSRV 53
P+G V+Q+VLE C D + + ++ I ++ L + YG ++V V
Sbjct: 59 PYGCRVIQRVLEHCKDPNTQQKVMDEILGAVSMLAQDQYGNYVVQHV 199
Score = 26.9 bits (58), Expect = 0.66
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +2
Query: 1 MQAMMKDPFGNYVV 14
+QAMMKD F NYVV
Sbjct: 383 LQAMMKDQFANYVV 424
>TC10753
Length = 568
Score = 28.9 bits (63), Expect = 0.17
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = +2
Query: 4 MMKDPFGNYVVQKVLETCDDQSLELILSRIKVHLNALKRYTYGKHIVSR 52
+++DP NYVVQ L+ + ++ I+ H + Y K I S+
Sbjct: 11 LLQDPHANYVVQSALKHSEGDVHNSLVEAIESHKAISRNSPYSKKIFSQ 157
>AV409964
Length = 434
Score = 25.0 bits (53), Expect = 2.5
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +3
Query: 3 AMMKDPFGNYVVQK 16
++M D FGNYV+QK
Sbjct: 393 SLMTDVFGNYVIQK 434
>TC14476 homologue to UP|TBB3_WHEAT (Q9ZRB0) Tubulin beta-3 chain (Beta-3
tubulin), complete
Length = 2208
Score = 24.6 bits (52), Expect = 3.3
Identities = 18/51 (35%), Positives = 24/51 (46%)
Frame = -2
Query: 33 IKVHLNALKRYTYGKHIVSRVEKLITTGGNECKLLRYMNSHLLSLNCSDFS 83
I VH+N LK +I S + + N KL + + L SLN DFS
Sbjct: 221 IVVHINPLKLKMRVAYITSTLIYAMFITHNLPKLASNLVATLASLNMKDFS 69
>TC7866 homologue to UP|SAHH_CATRO (P35007) Adenosylhomocysteinase
(S-adenosyl-L-homocysteine hydrolase) (AdoHcyase) ,
partial (38%)
Length = 561
Score = 24.6 bits (52), Expect = 3.3
Identities = 15/35 (42%), Positives = 20/35 (56%)
Frame = -3
Query: 48 HIVSRVEKLITTGGNECKLLRYMNSHLLSLNCSDF 82
H+V + L+T+GGNE LR NS L L+ F
Sbjct: 298 HVVYHDDVLVTSGGNEDVSLR--NSILQGLDLEAF 200
>TC15780 similar to UP|Q8VAD1 (Q8VAD1) Wsv494 (WSSV020), partial (29%)
Length = 653
Score = 24.3 bits (51), Expect = 4.3
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = -1
Query: 40 LKRYTYGKHIVS 51
LKRYT GKH++S
Sbjct: 455 LKRYTKGKHMIS 420
>BP060017
Length = 336
Score = 23.5 bits (49), Expect = 7.3
Identities = 10/21 (47%), Positives = 15/21 (70%), Gaps = 1/21 (4%)
Frame = -2
Query: 62 NECK-LLRYMNSHLLSLNCSD 81
N CK +RY+ + L+ +NCSD
Sbjct: 95 NLCK*FMRYLYAALIFINCSD 33
>TC17484 weakly similar to UP|DBAT_TAXCU (Q9M6E2) 10-deacetylbaccatin III
10-O-acetyltransferase (DBAT) , partial (11%)
Length = 577
Score = 23.5 bits (49), Expect = 7.3
Identities = 9/22 (40%), Positives = 14/22 (62%)
Frame = -3
Query: 7 DPFGNYVVQKVLETCDDQSLEL 28
+ FG + VQKV C++ L+L
Sbjct: 449 ETFGKWRVQKVSAICEEHKLQL 384
>TC16048 similar to UP|BAD08735 (BAD08735)
1-phosphatidylinositol-3-phosphate 5-kinase-like,
partial (7%)
Length = 691
Score = 23.5 bits (49), Expect = 7.3
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = +3
Query: 22 DDQSLELILSRIKVHLNALKRYTYGKHIVSRVEKLITTGG 61
D++ EL+L I + +++YT+ KH+ S V+ GG
Sbjct: 123 DEEKHELVLGII----DFMRQYTWDKHLESWVKTTGILGG 230
>BP047482
Length = 493
Score = 23.1 bits (48), Expect = 9.6
Identities = 12/34 (35%), Positives = 22/34 (64%), Gaps = 2/34 (5%)
Frame = -1
Query: 28 LILSRIKVHLNALKRYTY--GKHIVSRVEKLITT 59
LI+S K+HL LK+Y + G +S + +++T+
Sbjct: 433 LIISTEKLHLKKLKKYFHLMGMLKMSSLRQIVTS 332
>TC14805 similar to UP|RP3A_ARATH (Q39211) DNA-directed RNA polymerase II 36
kDa polypeptide A (RNA polymerase II subunit 3) ,
complete
Length = 1273
Score = 23.1 bits (48), Expect = 9.6
Identities = 7/39 (17%), Positives = 21/39 (52%)
Frame = +2
Query: 18 LETCDDQSLELILSRIKVHLNALKRYTYGKHIVSRVEKL 56
+E+C ++ + KV+++ + Y+Y ++ + E +
Sbjct: 674 VESCPTHVFDIDANTQKVYVHEAENYSYDDEVIKKAEAM 790
>TC14541 weakly similar to UP|Q8LPS4 (Q8LPS4) At3g06670/T8E24.10, partial
(3%)
Length = 1034
Score = 23.1 bits (48), Expect = 9.6
Identities = 15/49 (30%), Positives = 21/49 (42%)
Frame = -2
Query: 26 LELILSRIKVHLNALKRYTYGKHIVSRVEKLITTGGNECKLLRYMNSHL 74
L I + HL +K + +E+ I ECK LR + SHL
Sbjct: 484 LRYIALTVSNHLATIKS-------IHEIEQTICLHAYECKSLRSIYSHL 359
>BP049568
Length = 598
Score = 23.1 bits (48), Expect = 9.6
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = -1
Query: 25 SLELILSRIKVHLNALKRYTYGKHIVSRVEKLITTGGNECKLLRYMNSHLLS 76
SL + + + L LKRY K ++ V+ LI G KLL ++ + LL+
Sbjct: 424 SLGICIVVSALQLWRLKRYFQKKKLI*MVQVLI*D*GRNFKLLAFL*AILLT 269
>TC12625 similar to UP|Q84TG1 (Q84TG1) At3g09720, partial (34%)
Length = 549
Score = 23.1 bits (48), Expect = 9.6
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +2
Query: 54 EKLITTGGNECKLLRYMNSHLLSLN 78
+KL+ TG E KLL S SLN
Sbjct: 239 QKLVFTGSEEGKLLAIRQSFAESLN 313
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.136 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,472,364
Number of Sequences: 28460
Number of extensions: 16721
Number of successful extensions: 76
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 74
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 76
length of query: 83
length of database: 4,897,600
effective HSP length: 59
effective length of query: 24
effective length of database: 3,218,460
effective search space: 77243040
effective search space used: 77243040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)
Medicago: description of AC144720.4