BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144657.4 + phase: 0
(776 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AI967630 156 9e-64
TC18736 similar to UP|Q40485 (Q40485) C1C-Nt1 protein, partial (... 158 4e-39
TC9208 similar to UP|Q9XF71 (Q9XF71) CLC-Nt2 protein, partial (16%) 124 6e-29
TC11144 similar to UP|Q9XF71 (Q9XF71) CLC-Nt2 protein, partial (... 99 3e-21
AV414753 96 2e-20
TC13590 homologue to GB|AAC26247.2|6382041|F9D12 Arabidopsis tha... 40 0.002
AV406581 36 0.021
TC17205 weakly similar to PIR|S50830|S50830 Machado-Joseph disea... 32 0.50
BP042402 30 1.1
BP085151 30 1.1
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 30 1.5
BP082166 30 1.9
TC19184 weakly similar to UP|Q9FXH4 (Q9FXH4) F6F9.19 protein, pa... 29 2.5
AV407983 29 3.3
TC9408 homologue to UP|Q8H4V6 (Q8H4V6) OJ1484_G09.21 protein, pa... 28 7.3
AV422326 27 9.5
>AI967630
Length = 457
Score = 156 bits (394), Expect(2) = 9e-64
Identities = 78/90 (86%), Positives = 88/90 (97%)
Frame = +3
Query: 489 ERTNLSNGLYAVLGAASLLGGSMRTTVSLCVIMLELTNNLLLLPLIMMVLVVSKSVANVF 548
+++NL++GLYAVLGAAS LGGSMRTTVSLCVI+LELTNNLLLLPLIMMVL+VSKSVA+V
Sbjct: 15 KKSNLNHGLYAVLGAASFLGGSMRTTVSLCVIILELTNNLLLLPLIMMVLLVSKSVADVC 194
Query: 549 NANVYDLIMKAKGLPYLETHAEPYMRQLTV 578
NAN+YDLIMK+KGLPYLETHAEPYMRQLTV
Sbjct: 195 NANIYDLIMKSKGLPYLETHAEPYMRQLTV 284
Score = 105 bits (262), Expect(2) = 9e-64
Identities = 47/58 (81%), Positives = 55/58 (94%)
Frame = +1
Query: 578 VGDVVTGPLQMFNGIEKVRNIVFILRTTAHNGFPVIDEPPGSEAPILFGIILRHHLTT 635
+GDVVTGPLQ+F+G EKVRN+VF+L+TT+HNGFPVIDEPP SEAP+LFGIILRHHL T
Sbjct: 283 LGDVVTGPLQIFHGYEKVRNVVFVLKTTSHNGFPVIDEPPFSEAPVLFGIILRHHLLT 456
>TC18736 similar to UP|Q40485 (Q40485) C1C-Nt1 protein, partial (15%)
Length = 815
Score = 158 bits (399), Expect = 4e-39
Identities = 76/135 (56%), Positives = 97/135 (71%)
Frame = +1
Query: 625 FGIILRHHLTTLLKKKAFLPSPVANSYDVVRKFSSDDFAKKYSVERVKIEDIQLTEEEMG 684
FG++LR HL LLK K F V ++ K S DF K S + +K+ED+ L EEE+
Sbjct: 16 FGLVLRSHLLVLLKAKNFTRDRVFADQSIIDKVSVLDFGKAGSGKGIKLEDLDLEEEELD 195
Query: 685 MFVDLHPFTNASPYTVVETMSLAKALILFREVGLRHLLVIPKIPGRSPVVGILTRHDFTP 744
M+VDLHP TN+SP+TVVETMSLAKA I+FR+ GLRH+ V+PK GR PVVGILTRHDF P
Sbjct: 196 MYVDLHPITNSSPHTVVETMSLAKAAIIFRQQGLRHMCVVPKSQGRPPVVGILTRHDFMP 375
Query: 745 EHILGMHPFLVKSRW 759
EH+LG++P + +W
Sbjct: 376 EHVLGLYPDIKHHKW 420
>TC9208 similar to UP|Q9XF71 (Q9XF71) CLC-Nt2 protein, partial (16%)
Length = 613
Score = 124 bits (311), Expect = 6e-29
Identities = 71/139 (51%), Positives = 93/139 (66%), Gaps = 3/139 (2%)
Frame = +1
Query: 628 ILRHHLTTLLKKKAFLPSPV-ANSYDVVRKFSSDDFAKKYSVERVKIEDIQLTEEEMGMF 686
ILR HL LKKK FL ++V KF+S + A ER +E++ +T EEM MF
Sbjct: 1 ILRAHLIQALKKKWFLKERRRTEEWEVREKFTSVELA-----ERGNMEEVAITREEMEMF 165
Query: 687 VDLHPFTNASPYTVVETMSLAKALILFREVGLRHLLVIPK--IPGRSPVVGILTRHDFTP 744
VDLHP TN +P+TV+E++S+AKA+ILFR+VGLRHLLV+PK G SPV+GILTR D P
Sbjct: 166 VDLHPLTNTTPFTVLESVSVAKAMILFRQVGLRHLLVVPKYQASGVSPVIGILTRQDLLP 345
Query: 745 EHILGMHPFLVKSRWKRLR 763
+IL + P L K + + R
Sbjct: 346 YNILTVFPHLAKHKNREKR 402
>TC11144 similar to UP|Q9XF71 (Q9XF71) CLC-Nt2 protein, partial (14%)
Length = 557
Score = 99.0 bits (245), Expect = 3e-21
Identities = 51/119 (42%), Positives = 73/119 (60%)
Frame = +1
Query: 10 ESEPLLRRPLLSSQRSIINSTSQVAIVGANVCPIESLDYEIFENEFFKQDWRSRGVVQIL 69
E PL + L+ R++ S++ +A+VGA V IESLDYEI EN+ FK +WRSR V++L
Sbjct: 205 EGNPLNQPLLIKRNRTL--SSNPLALVGAKVSYIESLDYEINENDLFKHNWRSRSRVEVL 378
Query: 70 QYICMKWLLCFMIGLIVGFIGFCNNLAVENLAGIKFVTTSNMMLERRFMFAFFIFFASN 128
QYI +KW L ++GL+ G + NLAVEN+AG K + + R++ F F N
Sbjct: 379 QYIFLKWTLALLVGLLTGIMATLINLAVENIAGYKLLAVLRYIHTERYLAGFLYFTGVN 555
>AV414753
Length = 250
Score = 96.3 bits (238), Expect = 2e-20
Identities = 42/82 (51%), Positives = 57/82 (69%), Gaps = 3/82 (3%)
Frame = +2
Query: 381 APCRPC---PPDAVEPCPTIGRSGIYKKFQCPPNHYNGLASLIFNTNDDAIRNLFSMHTD 437
A C PC PPD+ CPT GRSG +K+F CPP HYN LA+L+ TNDDA+RN+FS +T
Sbjct: 2 AKCTPCGSLPPDSESVCPTNGRSGNFKQFNCPPGHYNDLATLLLTTNDDAVRNIFSTNTS 181
Query: 438 NEFELSSMLVFFIICLFLSIFS 459
E++ S+L+FF + L +F+
Sbjct: 182 QEYQPFSLLIFFALYCILGLFT 247
>TC13590 homologue to GB|AAC26247.2|6382041|F9D12 Arabidopsis thaliana CLC-d
chloride channel protein (GB:Z71450); coded for by A.
thaliana cDNA T44764 {Arabidopsis thaliana;} , partial
(5%)
Length = 501
Score = 39.7 bits (91), Expect = 0.002
Identities = 22/44 (50%), Positives = 29/44 (65%)
Frame = +3
Query: 702 ETMSLAKALILFREVGLRHLLVIPKIPGRSPVVGILTRHDFTPE 745
E MSL K LF ++GLRHL V+P+ P R V+G++TR D E
Sbjct: 3 EDMSLTKVYHLFXQLGLRHLFVVPR-PSR--VLGLITRKDLLIE 125
>AV406581
Length = 198
Score = 36.2 bits (82), Expect = 0.021
Identities = 20/47 (42%), Positives = 31/47 (65%)
Frame = +3
Query: 10 ESEPLLRRPLLSSQRSIINSTSQVAIVGANVCPIESLDYEIFENEFF 56
E +P+ + L+ R++ S++ +A+VGA V ESLDYEI EN+ F
Sbjct: 63 EGDPVNQPLLIKRNRTL--SSNPLALVGAKVWMEESLDYEINENDLF 197
>TC17205 weakly similar to PIR|S50830|S50830 Machado-Joseph disease MJD1a
protein - human {Homo sapiens;}, partial (7%)
Length = 528
Score = 31.6 bits (70), Expect = 0.50
Identities = 18/51 (35%), Positives = 35/51 (68%), Gaps = 1/51 (1%)
Frame = -2
Query: 493 LSNGLYAVLGAASLLGGSMRTTVSLCVIML-ELTNNLLLLPLIMMVLVVSK 542
L +G + +LG+A LL + ++ SL +I+L L LLL+PL++++ V+++
Sbjct: 458 LCHGEFFLLGSAELLSSAANSSSSLRLILLCALLLLLLLVPLLLLLFVLAE 306
>BP042402
Length = 409
Score = 30.4 bits (67), Expect = 1.1
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +3
Query: 375 FGLPWLAPCRPCPPDAVEPC 394
+GLPWL PCR PP + PC
Sbjct: 150 YGLPWLLPCR--PPGHLLPC 203
>BP085151
Length = 360
Score = 30.4 bits (67), Expect = 1.1
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +3
Query: 375 FGLPWLAPCRPCPPDAVEPC 394
+GLPWL PCR PP + PC
Sbjct: 147 YGLPWLLPCR--PPGHLLPC 200
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 30.0 bits (66), Expect = 1.5
Identities = 17/59 (28%), Positives = 26/59 (43%)
Frame = +2
Query: 694 NASPYTVVETMSLAKALILFREVGLRHLLVIPKIPGRSPVVGILTRHDFTPEHILGMHP 752
N + YT ++T + L+LF V+P +P P + L P H+L HP
Sbjct: 185 NPNSYTTIQTGGGSTQLMLFTSSSSWGAAVLPHLPHPPPRLHYLPLQHPLPPHLLHHHP 361
>BP082166
Length = 378
Score = 29.6 bits (65), Expect = 1.9
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = +3
Query: 105 FVTTSNMMLERRFMFAFFIFFASNLSLTLFASIITAFIAPT 145
F+T N F+ AFFI +LSL F I F +PT
Sbjct: 201 FLTDINFSASLCFLDAFFILIFFHLSLCFFFCFIIIFFSPT 323
>TC19184 weakly similar to UP|Q9FXH4 (Q9FXH4) F6F9.19 protein, partial (19%)
Length = 627
Score = 29.3 bits (64), Expect = 2.5
Identities = 25/101 (24%), Positives = 44/101 (42%), Gaps = 4/101 (3%)
Frame = +2
Query: 42 PIESLDYEIFENEFFKQD-WRSRGVVQILQYICMKWLLCFMIGLIVGFIG---FCNNLAV 97
P S+ EI E +D W S + I ++ + W LC + + VGF+ F
Sbjct: 233 PARSIADEIEEGAAEDEDTWSS*VLPLIESHLFLVWHLCHLCQMFVGFVNEGMFWFWRIY 412
Query: 98 ENLAGIKFVTTSNMMLERRFMFAFFIFFASNLSLTLFASII 138
E L G + TS+ +L F + F +++ L + ++
Sbjct: 413 EMLKGAAELYTSSSLLLYIVFFPLYFIFNQCINVELISVLL 535
>AV407983
Length = 417
Score = 28.9 bits (63), Expect = 3.3
Identities = 13/32 (40%), Positives = 17/32 (52%)
Frame = +2
Query: 616 PPGSEAPILFGIILRHHLTTLLKKKAFLPSPV 647
PP P F ++L H LL+ FLPSP+
Sbjct: 167 PPPPRTPRRFPLLLPRHTLPLLRHPFFLPSPL 262
>TC9408 homologue to UP|Q8H4V6 (Q8H4V6) OJ1484_G09.21 protein, partial (6%)
Length = 1105
Score = 27.7 bits (60), Expect = 7.3
Identities = 26/87 (29%), Positives = 39/87 (43%), Gaps = 1/87 (1%)
Frame = +3
Query: 435 HTDNEFELSSMLVF-FIICLFLSIFSCGIVAPAGLFVPIIVTGASYGRLVGILVGERTNL 493
HT + + + +F F+I + IFS + +P V +GAS G IL G T
Sbjct: 66 HTKSYHAIQTSNIFVFVIHIDWIIFSSAVSSPMAFTVAATGSGASSGG-AAILCGNSTTA 242
Query: 494 SNGLYAVLGAASLLGGSMRTTVSLCVI 520
+ Y L +L S RT SL ++
Sbjct: 243 TP--YTPLSHLALPSSSSRTRRSLHLV 317
>AV422326
Length = 483
Score = 27.3 bits (59), Expect = 9.5
Identities = 20/53 (37%), Positives = 27/53 (50%), Gaps = 3/53 (5%)
Frame = +1
Query: 446 LVFFIICLFLSIFSCGIVAPAGLFVPII---VTGASYGRLVGILVGERTNLSN 495
L F +I L I+ C + P + V I + GAS R + ILVG +NL N
Sbjct: 121 LCFLMIIYSLHIYYCAVFMP*KVIV*IFRKSI*GASS*RQIVILVGSVSNLWN 279
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.143 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,527,403
Number of Sequences: 28460
Number of extensions: 226345
Number of successful extensions: 1737
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1710
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1734
length of query: 776
length of database: 4,897,600
effective HSP length: 97
effective length of query: 679
effective length of database: 2,136,980
effective search space: 1451009420
effective search space used: 1451009420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC144657.4