BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144656.13 + phase: 0 /pseudo/partial
(1235 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG662087 126 2e-29
BP065719 64 1e-10
TC18698 58 1e-08
AV410603 46 4e-05
TC10048 similar to UP|Q9M7N4 (Q9M7N4) MFP1 attachment factor 1, ... 40 0.003
AV427333 38 0.009
AV419591 36 0.032
TC11113 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich... 36 0.032
TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycop... 36 0.042
TC14645 similar to UP|Q41122 (Q41122) Proline-rich protein precu... 35 0.072
TC14392 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, pa... 35 0.094
CN825556 34 0.12
TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protei... 34 0.16
TC18149 weakly similar to UP|Q91BL3 (Q91BL3) Essential structura... 33 0.21
TC17500 UP|Q9ZPL9 (Q9ZPL9) Nodule-enhanced protein phosphatase t... 33 0.27
BP043392 33 0.36
TC15072 33 0.36
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 32 0.47
AU251655 32 0.47
TC16697 32 0.61
>BG662087
Length = 373
Score = 126 bits (317), Expect = 2e-29
Identities = 61/119 (51%), Positives = 82/119 (68%)
Frame = +1
Query: 1105 GKVRMCVDFRDLNKASPKDNFPLLHIDVLVDNNAQSKVFFFMDGFSGYNQIKMSPEDREK 1164
GK RM VD+ DLNKA PKD++PL ID LVD + +++ MD +SGY+QIKM P D +K
Sbjct: 16 GKWRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDEDK 195
Query: 1165 TSFITPWGTFCYKVMPIGLINAGATYQRGMTTLFHDMIHKEVEVYVDDMIVKSKDEEQH 1223
T+F+T +CY+ +P GL NAGATYQ M +F D + + +EVY+D+MIVKS H
Sbjct: 196 TAFMTARVNYCYQTIPFGLKNAGATYQXLMDRVFXDXVGRNMEVYLDNMIVKSALRANH 372
>BP065719
Length = 567
Score = 63.9 bits (154), Expect = 1e-10
Identities = 37/128 (28%), Positives = 70/128 (53%), Gaps = 2/128 (1%)
Frame = +3
Query: 34 DVPKKFKVPEFDRYNGLTCPHN--HIIKYVRKMGNYSDNDSLMIHCFQDSLMEDAAEWYT 91
++P+ +KVP+F +++G + HI +Y + G+ + N++L + F SL ++A W+T
Sbjct: 159 ELPRGWKVPKFTKFSGDSGESTVEHIARYQIEAGDLAINENLKMKYFPSSLTKNAFTWFT 338
Query: 92 SLSKDDVHTFDELAAAFKSHYGFNTRLKPNREFLRSLSQKKDESFREYAQRWRGAAARIT 151
+L+ VHT+ +L F + F K + + L S+ +K ES +Y R+R +R
Sbjct: 339 TLAPRSVHTWAQLERIFHEQF-FRGECKVSXKDLASVKRKPAESIDDYLNRFRMLKSRCF 515
Query: 152 PALDEEEM 159
+ E E+
Sbjct: 516 THVSEHEL 539
>TC18698
Length = 808
Score = 57.8 bits (138), Expect = 1e-08
Identities = 31/62 (50%), Positives = 39/62 (62%)
Frame = -2
Query: 1162 REKTSFITPWGTFCYKVMPIGLINAGATYQRGMTTLFHDMIHKEVEVYVDDMIVKSKDEE 1221
++KT+ + Y+VMP+GL N TYQR M +FH I K VEVYV+DMIVKS E
Sbjct: 807 KKKTTLKINRVNYYYQVMPLGLKNI*TTYQRLMDKIFHKQI*KNVEVYVEDMIVKSSQE* 628
Query: 1222 QH 1223
H
Sbjct: 627 FH 622
>AV410603
Length = 162
Score = 45.8 bits (107), Expect = 4e-05
Identities = 24/51 (47%), Positives = 32/51 (62%)
Frame = +1
Query: 1122 KDNFPLLHIDVLVDNNAQSKVFFFMDGFSGYNQIKMSPEDREKTSFITPWG 1172
KD+FP+ +D L+D S+ F +D SGY+QI + PEDR KT F T G
Sbjct: 10 KDSFPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTHHG 162
>TC10048 similar to UP|Q9M7N4 (Q9M7N4) MFP1 attachment factor 1, partial
(66%)
Length = 718
Score = 39.7 bits (91), Expect = 0.003
Identities = 41/130 (31%), Positives = 51/130 (38%), Gaps = 29/130 (22%)
Frame = +2
Query: 251 LPPSYPYAPYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQARPTFPPI-PM 309
LPPS P P S PYP P P +A Q + +PP + RP PP+ P
Sbjct: 158 LPPSAPATPSSTA*SKPYPPPLSSP----SATEQCLPMRPP-PLLARSRMRPLLPPVAPP 322
Query: 310 LYAELLPTLLLRGHCTTRQD----------KPP---------------PDPLPPRF---R 341
L L+PT CT R+ +PP P PLP R +
Sbjct: 323 L---LIPTASRSSRCTPRRSASGCSIVSSPEPPQVPPPLITVLLMFLLPLPLPKRMLLPQ 493
Query: 342 SDLKCDFHQG 351
LK D+HQG
Sbjct: 494 LILKPDYHQG 523
>AV427333
Length = 387
Score = 38.1 bits (87), Expect = 0.009
Identities = 31/96 (32%), Positives = 39/96 (40%), Gaps = 9/96 (9%)
Frame = +3
Query: 252 PPSYPYAPYS--QHPFFPYPLPSG---QPQVPVNAVVQQMQ--QQPPVQQQQHQQARPTF 304
PPS+PY P + HPF P P PS P P ++ + PP + P
Sbjct: 87 PPSHPYNPPTPPSHPFNPPPTPSHYFKSPPPPSHSFAPPPRGHPSPPPSSPPPPSSHPFT 266
Query: 305 PPIPMLYAELLPTLLLRGHCTTRQD--KPPPDPLPP 338
PP P + P H T +PPP PLPP
Sbjct: 267 PPPPHVRPPPSPH-----HPITPPPHVRPPPPPLPP 359
Score = 28.1 bits (61), Expect = 8.8
Identities = 19/57 (33%), Positives = 22/57 (38%)
Frame = +3
Query: 252 PPSYPYAPYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQARPTFPPIP 308
PPS P P S HPF P P P P + + +PP P PP P
Sbjct: 225 PPSSPPPP-SSHPFTPPPPHVRPPPSPHHPITPPPHVRPP---------PPPLPPSP 365
>AV419591
Length = 276
Score = 36.2 bits (82), Expect = 0.032
Identities = 22/58 (37%), Positives = 27/58 (45%), Gaps = 2/58 (3%)
Frame = +2
Query: 252 PPSYPY--APYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQARPTFPPI 307
PPS P P SQ P P P P QP++PV QPPV + +P F P+
Sbjct: 92 PPSQPRLAVPCSQPPVVPRPPPLPQPRLPVRC------SQPPVVPRPPPLPQPLFSPV 247
>TC11113 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich
glycoprotein DZ-HRGP precursor, partial (10%)
Length = 309
Score = 36.2 bits (82), Expect = 0.032
Identities = 22/89 (24%), Positives = 33/89 (36%)
Frame = +1
Query: 252 PPSYPYAPYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQARPTFPPIPMLY 311
P S+ ++ HP+ P+P+ + ++ QPP H P PP P L
Sbjct: 28 PHSHSHSHSHSHPWLPFPISA------------PLRSQPPSTPAVHHSPLPYLPPSPSLP 171
Query: 312 AELLPTLLLRGHCTTRQDKPPPDPLPPRF 340
P H + + PPP P F
Sbjct: 172 NSPPPPPRSGHHHPSPSEPPPPPQTTPPF 258
>TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycoprotein
DZ-HRGP precursor, partial (8%)
Length = 584
Score = 35.8 bits (81), Expect = 0.042
Identities = 25/85 (29%), Positives = 30/85 (34%)
Frame = +1
Query: 255 YPYAPYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQARPTFPPIPMLYAEL 314
YP PY QH F+P P+ P P A P + A P P P L+
Sbjct: 202 YPSLPYPQHQFYPPPMAPPPPPPPAPA---------PPAPPPYWAAPPPRPYGPPLFTST 354
Query: 315 LPTLLLRGHCTTRQDKPPPDPLPPR 339
LP PP P PP+
Sbjct: 355 LP-------------PRPPKPAPPK 390
>TC14645 similar to UP|Q41122 (Q41122) Proline-rich protein precursor,
partial (52%)
Length = 1060
Score = 35.0 bits (79), Expect = 0.072
Identities = 28/87 (32%), Positives = 38/87 (43%), Gaps = 3/87 (3%)
Frame = +2
Query: 243 VAPINAA*LPPSYP--YAPYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQA 300
VAP++ P +P +AP+ H P P P+ P P + +PP H
Sbjct: 227 VAPVHPPTHSPIHPPAHAPHHHHHHHP-PAPAPAPIKPPVHPPTSVPVKPPTGHHHHHHP 403
Query: 301 RPTFPPIPMLYAEL-LPTLLLRGHCTT 326
P+ P P LY + LP LLL H T
Sbjct: 404 -PSPAPAPALYTTITLPPLLLFTHLFT 481
>TC14392 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, partial (77%)
Length = 1576
Score = 34.7 bits (78), Expect = 0.094
Identities = 23/60 (38%), Positives = 27/60 (44%), Gaps = 6/60 (10%)
Frame = +3
Query: 252 PPSYPYAPY--SQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQ----QQHQQARPTFP 305
PP P Y Q P+ P QPQ P + QPP QQ QQHQ A+P+ P
Sbjct: 150 PPQQPNQQYQQQQQPYMMMPPQQHQPQPP--TMWAPSAAQPPQQQHAPPQQHQHAQPSTP 323
>CN825556
Length = 655
Score = 34.3 bits (77), Expect = 0.12
Identities = 24/66 (36%), Positives = 26/66 (39%)
Frame = +1
Query: 251 LPPSYPYAPYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQARPTFPPIPML 310
LPP P P P P P P QPQ Q Q QP Q Q Q +P PP +
Sbjct: 424 LPPPQPVPP---PPSLPQPQPQPQPQPQ-----PQPQPQPQPQPQPQPQPQPVPPPASLP 579
Query: 311 YAELLP 316
L P
Sbjct: 580 QPLLAP 597
>TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protein, partial
(25%)
Length = 1077
Score = 33.9 bits (76), Expect = 0.16
Identities = 24/85 (28%), Positives = 30/85 (35%)
Frame = +1
Query: 256 PYAPYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQARPTFPPIPMLYAELL 315
P+ P S P FP P P + Q PP Q PP P+
Sbjct: 547 PFQPPSPPPLFPNPFQPPSPPPLIPNPFQPPPSPPPFIPNPFQPPPSKQPPSPLF---PF 717
Query: 316 PTLLLRGHCTTRQDKPPPDPLPPRF 340
P +++ G PPP P PP F
Sbjct: 718 PPIVIPG-----LTPPPPPPPPPPF 777
>TC18149 weakly similar to UP|Q91BL3 (Q91BL3) Essential structural protein
pp78/81, partial (5%)
Length = 710
Score = 33.5 bits (75), Expect = 0.21
Identities = 40/134 (29%), Positives = 49/134 (35%), Gaps = 30/134 (22%)
Frame = +3
Query: 238 QSMATVAPINAA*-------LPPSYPY----------APYSQHPFFPYPL-----PSGQP 275
QS+ V P NA LPPS+P A + P+FP P+ P+ Q
Sbjct: 99 QSIPVVPPPNAPPQLPPQQGLPPSFPPPNQFSQTQIPAVPQRDPYFPPPVQSQETPNQQY 278
Query: 276 QVPVNAVVQQMQQQPPVQQQQH-------QQARPTF-PPIPMLYAELLPTLLLRGHCTTR 327
Q P+ PP QQ Q Q A P PPIP + L + L GH
Sbjct: 279 QQPLAPQPHPQPGAPPHQQYQQTPHPQYSQPAPPQHQPPIPSINPPQLQSSL--GHHVEE 452
Query: 328 QDKPPPDPLPPRFR 341
P PP R
Sbjct: 453 PPYVPSQTYPPNLR 494
>TC17500 UP|Q9ZPL9 (Q9ZPL9) Nodule-enhanced protein phosphatase type 2C,
complete
Length = 1325
Score = 33.1 bits (74), Expect = 0.27
Identities = 25/86 (29%), Positives = 31/86 (35%)
Frame = -3
Query: 252 PPSYPYAPYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQARPTFPPIPMLY 311
P S P+ P P++ LPS P P + Q PP Q A P PP P L
Sbjct: 672 PSSTPHYPPPHRPYYENTLPSLPPSPPPPPHSRTSQPPPP----QADTAAPGTPPPPELR 505
Query: 312 AELLPTLLLRGHCTTRQDKPPPDPLP 337
+ R T + P P P
Sbjct: 504 RDRQKQRSSRTSSRTLSPRSPRPPSP 427
>BP043392
Length = 442
Score = 32.7 bits (73), Expect = 0.36
Identities = 22/60 (36%), Positives = 24/60 (39%), Gaps = 3/60 (5%)
Frame = -2
Query: 252 PPSYPYAP-YSQHPFFPYPLPSGQPQVPV--NAVVQQMQQQPPVQQQQHQQARPTFPPIP 308
PPSY Y P Y+ P P P P Q P N PP Q R T PP+P
Sbjct: 414 PPSYGYPPYYNAVPPPPPPAPVTSEQSPSFGNVPWASNPMVPPPPSDNQSQPRATNPPLP 235
>TC15072
Length = 721
Score = 32.7 bits (73), Expect = 0.36
Identities = 22/60 (36%), Positives = 24/60 (39%), Gaps = 3/60 (5%)
Frame = +1
Query: 252 PPSYPYAP-YSQHPFFPYPLPSGQPQVPV--NAVVQQMQQQPPVQQQQHQQARPTFPPIP 308
PPSY Y P Y+ P P P P Q P N PP Q R T PP+P
Sbjct: 31 PPSYGYPPYYNAVPPPPPPAPVTSEQSPSFGNVPWASNPMVPPPPSDNQSQPRATNPPLP 210
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 32.3 bits (72), Expect = 0.47
Identities = 25/88 (28%), Positives = 29/88 (32%)
Frame = -1
Query: 252 PPSYPYAPYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQARPTFPPIPMLY 311
PPS PY P + P P P P P P PP + P +PP P
Sbjct: 506 PPSPPYPPSRRPP*PPPPPPPYPPPPP----P*PRDPPPPPYPPSRRPPPPPYPPPP--- 348
Query: 312 AELLPTLLLRGHCTTRQDKPPPDPLPPR 339
+PPP P PPR
Sbjct: 347 ------------------RPPPPPPPPR 318
>AU251655
Length = 397
Score = 32.3 bits (72), Expect = 0.47
Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +3
Query: 931 MHVSELNHNKPVGHSNPTFPPNFEFPMYEAE-AEEGDDIPY 970
+H++ELN N+ H N P F F EA +E GD P+
Sbjct: 51 IHLTELNDNEKNNHGNQQLNPVFAFDQKEAAMSEAGDSAPF 173
>TC16697
Length = 635
Score = 32.0 bits (71), Expect = 0.61
Identities = 24/86 (27%), Positives = 30/86 (33%)
Frame = -1
Query: 252 PPSYPYAPYSQHPFFPYPLPSGQPQVPVNAVVQQMQQQPPVQQQQHQQARPTFPPIPMLY 311
PP++P P S P P PS + P A Q PP P P P
Sbjct: 572 PPNHPPPPSSPSP----PAPSQRKPAPATAPPSNSSQSPPPNPSSTDPTSPAPQPHP--- 414
Query: 312 AELLPTLLLRGHCTTRQDKPPPDPLP 337
+ L P ++ Q P P P P
Sbjct: 413 SHLSPA------SSSSQGSPVPSPSP 354
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,035,537
Number of Sequences: 28460
Number of extensions: 315117
Number of successful extensions: 2277
Number of sequences better than 10.0: 79
Number of HSP's better than 10.0 without gapping: 2098
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2231
length of query: 1235
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1134
effective length of database: 2,023,140
effective search space: 2294240760
effective search space used: 2294240760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144656.13