BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144645.3 + phase: 0 /pseudo
(993 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV766088 179 1e-45
BP066094 116 1e-26
BP063902 97 2e-20
BP063694 93 2e-19
AU088980 77 1e-14
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 70 1e-12
BP033602 67 1e-11
AV767042 64 2e-10
BP058111 57 1e-08
AV427921 55 7e-08
AV769206 54 2e-07
AV424544 51 8e-07
BP030995 51 1e-06
AV764934 47 2e-05
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 45 4e-05
BP054557 45 7e-05
BP081717 44 2e-04
AV780009 43 2e-04
BP043465 40 0.002
BP073434 39 0.004
>AV766088
Length = 501
Score = 179 bits (455), Expect = 1e-45
Identities = 78/139 (56%), Positives = 106/139 (76%)
Frame = -3
Query: 87 QTGIPLMRCDSTGDLYPLTTTTSHQSTSPATFAALSPTLWHNRLGHPGANVLSFLNKNKF 146
QTGIPLMRC+S GDLYP+T + FA ++ +LWH+RLGHP ++ L +L NKF
Sbjct: 397 QTGIPLMRCNSPGDLYPVTLSFP--------FAGIAQSLWHSRLGHPSSSALRYLRSNKF 242
Query: 147 IECKQISSPSICQSCIYGKHVKLPFSISNSTTSKPFDIIHSDLWTSPILSSAGHKYYLFF 206
I + ++ +C+SC++GKHV+LPF SN+ T PFDI+HSDLWTSP+LSSAGH++Y+ F
Sbjct: 241 ISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMPFDILHSDLWTSPVLSSAGHRFYVLF 62
Query: 207 LDDYTNFVWTFPIGRKSQV 225
LDD+T+F+WTFP+ KSQV
Sbjct: 61 LDDFTDFLWTFPLSNKSQV 5
>BP066094
Length = 532
Score = 116 bits (291), Expect(2) = 1e-26
Identities = 56/144 (38%), Positives = 90/144 (61%)
Frame = +2
Query: 622 KPTTIRIVLTIALSKSWPIHQLDVKNVFLHGELQETVYMHQPMGFRDPIHPDYVCLLKKS 681
K IR++++ +++ + +HQ++VK+ FL+G + E VY+HQP G D + D++ LKKS
Sbjct: 89 KTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKKS 268
Query: 682 LYGLKQAPRAWYQRFADFAFTIGFSHSKSDHSLFIYRKGNDMTYILLYVDDIILTASSDV 741
LYGLKQAPRAWY+R + F K D +LF +D+ + +YVDDII +++
Sbjct: 269 LYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANPS 448
Query: 742 LRRSIMSLLASEFAMKDLGTLSYF 765
L + ++ +EF M+ +G L YF
Sbjct: 449 LCKEFSEMMQAEFEMRMMGELKYF 520
Score = 21.2 bits (43), Expect(2) = 1e-26
Identities = 9/17 (52%), Positives = 12/17 (69%)
Frame = +3
Query: 606 SQ*VGVDCDETFSPVVK 622
SQ G+D ETF+PV +
Sbjct: 42 SQQ*GIDYTETFAPVAR 92
>BP063902
Length = 494
Score = 96.7 bits (239), Expect = 2e-20
Identities = 43/89 (48%), Positives = 62/89 (69%)
Frame = -3
Query: 86 IQTGIPLMRCDSTGDLYPLTTTTSHQSTSPATFAALSPTLWHNRLGHPGANVLSFLNKNK 145
++TG+PL+RC+S GDLYP+T ++ FA L+ ++WH+RLGHP ++ L+ L NK
Sbjct: 276 LKTGMPLLRCNSLGDLYPVTRSSP--------FAGLASSVWHHRLGHPASSALNHLRNNK 121
Query: 146 FIECKQISSPSICQSCIYGKHVKLPFSIS 174
I C+ S S+C SC+ GKHV+LPFS S
Sbjct: 120 LIFCEPSRSSSVCDSCVLGKHVRLPFSSS 34
>BP063694
Length = 511
Score = 93.2 bits (230), Expect = 2e-19
Identities = 52/163 (31%), Positives = 82/163 (49%), Gaps = 2/163 (1%)
Frame = -2
Query: 80 SFSVEDIQTGIPLMRCDSTGDLYPLTTTTSHQSTSPATFAALSPTLWHNRLGHPGANVLS 139
SF +++ +TG L R LY L + T+ + S LWH+RLGH +++
Sbjct: 495 SFVIQNRKTGAVLGRXRCDQGLYVLNQDSQALLTTSSPLPRASFELWHSRLGHVNFDIIK 316
Query: 140 FLNKNKFIECKQI-SSPSICQSCIYGKHVKLPFSISNSTTSKPFDIIHSDL-WTSPILSS 197
L+K+ ++ I P C SC K +L F +N S D+IH DL SP+ S
Sbjct: 315 QLHKHGCLDVSSILPKPICCTSCQMAKSKRLVFHDNNKRASAVLDLIHCDLRGPSPVASI 136
Query: 198 AGHKYYLFFLDDYTNFVWTFPIGRKSQVPSIFSSFHAFIKTQF 240
G Y++ F+DD++ F W +P+ RKS + F F++ +F
Sbjct: 135 DGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFMENRF 7
>AU088980
Length = 360
Score = 77.4 bits (189), Expect = 1e-14
Identities = 43/115 (37%), Positives = 57/115 (49%)
Frame = +2
Query: 282 NGKAERKIRAINNFIRTSLAHSSMPPSFWHHALQITTYLQNILPSKILSHHSPTQYLYHR 341
N ERK R I N R + HS +P + W A++ YL N LPS +L P Q+L +
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 342 DPSYTHLRVFGCLCYPLFPSTAINKLQARSTPCAFLGYPQNHRGYKCYDLSSKKI 396
P+ L+VFG LCY + KL R+ C FLG+ GY DL + I
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDLPTTXI 346
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 70.5 bits (171), Expect = 1e-12
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = +3
Query: 154 SPSICQSCIYGKHVKLPFSISNSTTSKPFDIIHSDLWTSPILS 196
S S+C SC+ GKHV+LPFS S + T +PFDI+HSDLWTSP+LS
Sbjct: 1017 SNSVCDSCVLGKHVRLPFSSSETITLRPFDILHSDLWTSPVLS 1145
>BP033602
Length = 533
Score = 67.4 bits (163), Expect = 1e-11
Identities = 34/89 (38%), Positives = 53/89 (59%), Gaps = 2/89 (2%)
Frame = +3
Query: 125 LWHNRLGHPGANVLSFLNKNKFIECKQISSPSI-CQSCIYGKHVKLPFSISNSTTSKPFD 183
LWHNRLGHP L L + F K ++ S+ C+SC+ K+ + P+ S+PF
Sbjct: 276 LWHNRLGHPNFQYLRHLFPDLF---KNVNCSSLECESCVLAKNQRAPYYSQPYHASRPFY 446
Query: 184 IIHSDLW-TSPILSSAGHKYYLFFLDDYT 211
+IHSD+W S I + G ++++ F+DD+T
Sbjct: 447 LIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>AV767042
Length = 444
Score = 63.5 bits (153), Expect = 2e-10
Identities = 29/44 (65%), Positives = 37/44 (83%)
Frame = -3
Query: 607 Q*VGVDCDETFSPVVKPTTIRIVLTIALSKSWPIHQLDVKNVFL 650
Q GVDC++TFSPVVKP IR V TIALS+SWPI QL+V+N+++
Sbjct: 349 QTAGVDCNKTFSPVVKPAPIRTVFTIALSRSWPIPQLNVQNLWI 218
>BP058111
Length = 570
Score = 57.4 bits (137), Expect = 1e-08
Identities = 37/104 (35%), Positives = 48/104 (45%), Gaps = 12/104 (11%)
Frame = -1
Query: 96 DSTGDLYPLTTTTSHQSTSPA-----------TFAALSPTLWHNRLGHPGANVLSFLNKN 144
+ GDLY L P F LWH RLGH + L+ L KN
Sbjct: 306 EERGDLYALIDPAVKVKHPPVEDNIKTQVSHFNFCQFDCNLWHLRLGHTSSKKLAILQKN 127
Query: 145 -KFIECKQISSPSICQSCIYGKHVKLPFSISNSTTSKPFDIIHS 187
FI CK+I+SP C +C K KL F S + +S+ FD+IH+
Sbjct: 126 FPFITCKKITSP--CDTCHMAKQKKLSFPNSVTLSSEIFDLIHT 1
>AV427921
Length = 284
Score = 54.7 bits (130), Expect = 7e-08
Identities = 28/84 (33%), Positives = 44/84 (52%)
Frame = +1
Query: 277 HTSPQNGKAERKIRAINNFIRTSLAHSSMPPSFWHHALQITTYLQNILPSKILSHHSPTQ 336
+T QNG +ERK R I N +R+ L S +P SF A+ + ++ N P+ ++ + P +
Sbjct: 19 YTPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEE 198
Query: 337 YLYHRDPSYTHLRVFGCLCYPLFP 360
R P+ H R+ CL Y P
Sbjct: 199 AWSGRQPAVDHFRISRCLAYAHVP 270
>AV769206
Length = 536
Score = 53.5 bits (127), Expect = 2e-07
Identities = 29/73 (39%), Positives = 42/73 (56%), Gaps = 3/73 (4%)
Frame = -3
Query: 366 KLQARSTPCAFLGYPQNHRGYKCYDLSSKKIIISRHVIFDETQFPFAK---THTSSTHTY 422
KL S C F+GY NH+GYKC D S +I IS+ V+F E +FP+ T+S+ +
Sbjct: 510 KLSLHSKECVFIGYSINHKGYKCLD-QSGRIYISKDVLFHEHRFPYPSLFPDETTSSTSA 334
Query: 423 EFLNDSLHPLLHY 435
EF S P++ +
Sbjct: 333 EFFPLSTIPIVSH 295
>AV424544
Length = 276
Score = 51.2 bits (121), Expect = 8e-07
Identities = 26/71 (36%), Positives = 40/71 (55%)
Frame = +3
Query: 695 RFADFAFTIGFSHSKSDHSLFIYRKGNDMTYILLYVDDIILTASSDVLRRSIMSLLASEF 754
+ + + +G+ S DHSLF + T IL+YVDD+IL + + + + L +F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 755 AMKDLGTLSYF 765
+KDLGTL YF
Sbjct: 186 RIKDLGTLKYF 218
>BP030995
Length = 458
Score = 50.8 bits (120), Expect = 1e-06
Identities = 42/139 (30%), Positives = 61/139 (43%), Gaps = 28/139 (20%)
Frame = -3
Query: 44 PLSLNNVLHAPKLIKNLIFVRRFTIDNNVTIEFDPFSFSVEDIQTGIPLMR-------CD 96
PL LNN+LH P + KNL V F NNV EF S V+ +T L++ C
Sbjct: 429 PLVLNNLLHVPGVCKNLSSVSXFCKQNNVFFEFHSDSCLVKSQETEETLLQGTLRNGLCA 250
Query: 97 STGDLY--------PLTTTTSHQSTSPATFAALS-------------PTLWHNRLGHPGA 135
L+ L+ + S+SPA+ ++S LWH+RLGH
Sbjct: 249 FDNLLHKVNLHSASTLSNSALLSSSSPASALSVSVFSSNKCAQADIDSRLWHSRLGHASQ 70
Query: 136 NVLSFLNKNKFIECKQISS 154
++S + K+ + C I S
Sbjct: 69 KIVSHVLKHVILLCH*IKS 13
>AV764934
Length = 406
Score = 46.6 bits (109), Expect = 2e-05
Identities = 29/85 (34%), Positives = 46/85 (54%), Gaps = 1/85 (1%)
Frame = -2
Query: 113 TSPATFAALSPTLWHNRLGHPGANVLSFLNKNKFIECKQISSPSICQSCIYGKHVKLPFS 172
+S + F LS WH+RLGHP + L + + + + +C GK +LP
Sbjct: 246 SSVSAFVTLST--WHSRLGHPHLDNLKRVLSSCNVSYSPKDTVEFRTACCLGKAHRLPSQ 73
Query: 173 ISNSTTSKPFDIIHSDLW-TSPILS 196
+S +TT PF++I+SDLW +P+ S
Sbjct: 72 MS-TTTYLPFELIYSDLWGPAPVCS 1
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 45.4 bits (106), Expect = 4e-05
Identities = 24/66 (36%), Positives = 37/66 (55%), Gaps = 1/66 (1%)
Frame = +2
Query: 696 FADFAFTIGFSHSKSDHSLFIYR-KGNDMTYILLYVDDIILTASSDVLRRSIMSLLASEF 754
F F ++G++ SDH + R ND +LLYVDD+++ + + + + LA EF
Sbjct: 2 FDSFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREF 181
Query: 755 AMKDLG 760
MKDLG
Sbjct: 182 DMKDLG 199
>BP054557
Length = 538
Score = 44.7 bits (104), Expect = 7e-05
Identities = 37/127 (29%), Positives = 49/127 (38%), Gaps = 12/127 (9%)
Frame = -2
Query: 47 LNNVLHAPKLIKNLIFVRRFTIDNNVTIEFDPFSFSVEDIQTGIPLMRCDSTGDLYPLTT 106
L VL+ P NLI V N + +++ T + + G LY +
Sbjct: 384 LAXVLYVPSFNFNLISVSALVKSLNCRLVIFDECCLLQERPTLKTIGAAEERGGLYAVID 205
Query: 107 TTSHQSTSPA-----------TFAALSPTLWHNRLGHPGANVLSFLNKN-KFIECKQISS 154
PA F LWH RLGH + L+ L KN FI CKQI+S
Sbjct: 204 PAVKAKHPPAEGNIKTQVSHFNFCQFDCNLWHLRLGHTSSKKLAILQKNFPFITCKQITS 25
Query: 155 PSICQSC 161
P C +C
Sbjct: 24 P--CDTC 10
>BP081717
Length = 291
Score = 43.5 bits (101), Expect = 2e-04
Identities = 22/58 (37%), Positives = 31/58 (52%)
Frame = -1
Query: 125 LWHNRLGHPGANVLSFLNKNKFIECKQISSPSICQSCIYGKHVKLPFSISNSTTSKPF 182
+WH RLGHP + VL + +K ++ S IC C K KLPF IS++ + F
Sbjct: 168 MWHFRLGHPSSKVLQHI--SKVFPYVKLHSNIICDFCHMAKQTKLPFPISDTKSKACF 1
>AV780009
Length = 529
Score = 43.1 bits (100), Expect = 2e-04
Identities = 24/68 (35%), Positives = 34/68 (49%)
Frame = -3
Query: 826 LGWVPRY*TFHLWILHLPWRQLDIMVI*ASTNSISIKC*SRI*RGCKCCL*ILLDPKPSL 885
LGW+PRY TF WI H+ W + M + + S * R+* C C+* P L
Sbjct: 425 LGWLPRYSTFCHWIFHISWHLSNFMAHEETNHCFSFIL*GRV*GPCCYCV*---SPVALL 255
Query: 886 GTPLPNSE 893
P+P ++
Sbjct: 254 FVPVPQAQ 231
>BP043465
Length = 559
Score = 40.0 bits (92), Expect = 0.002
Identities = 29/96 (30%), Positives = 42/96 (43%)
Frame = +2
Query: 417 SSTHTYEFLNDSLHPLLHYHLQNDPKQDEPEPRKIESPQPATTPASPINVTNQSILPPSP 476
S ++T +F N +L H+H PR+I P P + A P + + S+ P P
Sbjct: 41 SLSYTLQFANPTLLSPSHFH-----------PRRIRIPPPQSASAPPFSDLSLSVSPIPP 187
Query: 477 MSINQLPHPLVSTELTSPTHTPQQIHQEPPRTIATH 512
+S N HP + SPT I P I+TH
Sbjct: 188 IS-NSTTHPWATAR*ISPT-----ISSPPSLPISTH 277
>BP073434
Length = 490
Score = 38.9 bits (89), Expect = 0.004
Identities = 19/55 (34%), Positives = 27/55 (48%)
Frame = -1
Query: 125 LWHNRLGHPGANVLSFLNKNKFIECKQISSPSICQSCIYGKHVKLPFSISNSTTS 179
+WH+RLGHP + L + ++ I C +C GK LP S+S S S
Sbjct: 175 IWHSRLGHPHQDALQAVLQSCNIHLSSRDMTDFCYACCLGKSHILPSSLSTSVYS 11
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.332 0.142 0.470
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,231,032
Number of Sequences: 28460
Number of extensions: 418654
Number of successful extensions: 3917
Number of sequences better than 10.0: 197
Number of HSP's better than 10.0 without gapping: 3596
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3830
length of query: 993
length of database: 4,897,600
effective HSP length: 99
effective length of query: 894
effective length of database: 2,080,060
effective search space: 1859573640
effective search space used: 1859573640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144645.3