BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144644.6 + phase: 0
(235 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18770 similar to UP|Q7Y0Q8 (Q7Y0Q8) Rac GDP-dissociation inhib... 389 e-109
TC17933 homologue to UP|CAF02296 (CAF02296) Rho GDP dissociation... 131 9e-32
TC8108 homologue to UP|CAF02295 (CAF02295) Rho GDP dissociation ... 72 6e-14
AV779190 30 0.36
TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Ar... 29 0.62
BP034655 28 1.8
TC9414 similar to UP|RNPA_SYNPX (Q7U524) Ribonuclease P protein ... 28 1.8
TC17637 27 3.1
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 27 3.1
TC8557 similar to UP|Q9LK52 (Q9LK52) Dbj|BAA90629.1, complete 27 3.1
TC19471 27 4.0
TC12700 similar to UP|P93390 (P93390) Phosphate/phosphoenolpyruv... 27 4.0
TC18481 similar to UP|O23166 (O23166) Thiol-disulfide interchang... 26 5.2
AV416122 26 6.8
BI420048 26 6.8
CB828220 25 8.9
>TC18770 similar to UP|Q7Y0Q8 (Q7Y0Q8) Rac GDP-dissociation inhibitor 1,
partial (66%)
Length = 948
Score = 389 bits (998), Expect = e-109
Identities = 199/242 (82%), Positives = 214/242 (88%), Gaps = 7/242 (2%)
Frame = +1
Query: 1 MSAAVGAVSTTKDVSFNAHLEEEHKNKGTNKVGVNYDE------AEEIVPDEDEEEEPKL 54
MSAAVGAVSTTKDV+FNAHLEEE KNKG NKV D+ AEE E EEE+ KL
Sbjct: 136 MSAAVGAVSTTKDVTFNAHLEEELKNKGINKVDEEQDQLLYNDAAEET---ELEEEDSKL 306
Query: 55 ELDLGPQFSLKEQLEKDKDDESLRKWKEQLLGNVDVSAVGEKIEPEVKIVSLTIICQGRP 114
ELDLGPQFSLKEQLEKDKDDESLRKWKEQLLG+VD+ AVGE EPEVKI+SLTIICQGRP
Sbjct: 307 ELDLGPQFSLKEQLEKDKDDESLRKWKEQLLGSVDMPAVGENKEPEVKILSLTIICQGRP 486
Query: 115 DLILPIPFTADSKKS-MFTLKEGSQYRLKFSFTVSNNIVSGLRYTNVVWKTGVRVENTKK 173
+L+LPIPFT +SKKS +FTLKEGS+YRLKFSFTVSNNIVSGL+YTNVVWKTGVRV+N K+
Sbjct: 487 ELLLPIPFTTNSKKSSIFTLKEGSRYRLKFSFTVSNNIVSGLKYTNVVWKTGVRVDNAKR 666
Query: 174 MLGTYSPQQEPYTYELEEEITPSGLFARGTYSARTKFVDDDRKCYLDASYRFEIQKNWPT 233
MLGTYSPQQEPYTYELEEE TPSGLFARGTYSARTKFVDDDRKCYLDA+Y FEIQKNWP
Sbjct: 667 MLGTYSPQQEPYTYELEEETTPSGLFARGTYSARTKFVDDDRKCYLDATYHFEIQKNWPA 846
Query: 234 PH 235
H
Sbjct: 847 AH 852
>TC17933 homologue to UP|CAF02296 (CAF02296) Rho GDP dissociation inhibitor
2, partial (34%)
Length = 482
Score = 131 bits (330), Expect = 9e-32
Identities = 56/80 (70%), Positives = 72/80 (90%)
Frame = +1
Query: 152 VSGLRYTNVVWKTGVRVENTKKMLGTYSPQQEPYTYELEEEITPSGLFARGTYSARTKFV 211
VSGL+YTN VWKTG++V++TK+M+GT+SPQ EPYT+E+ EE TPSG+FARGTYSARTKFV
Sbjct: 1 VSGLKYTNTVWKTGIKVDSTKEMIGTFSPQAEPYTHEMPEETTPSGIFARGTYSARTKFV 180
Query: 212 DDDRKCYLDASYRFEIQKNW 231
DDD K YL+ +Y F+I+K+W
Sbjct: 181 DDDNKMYLEINYTFDIRKDW 240
>TC8108 homologue to UP|CAF02295 (CAF02295) Rho GDP dissociation inhibitor
1, partial (28%)
Length = 599
Score = 72.4 bits (176), Expect = 6e-14
Identities = 30/51 (58%), Positives = 41/51 (79%)
Frame = +2
Query: 168 VENTKKMLGTYSPQQEPYTYELEEEITPSGLFARGTYSARTKFVDDDRKCY 218
V+ +K MLGT++PQ+EPY Y L+E+ TPSG ARG YSA+ KF DDD++C+
Sbjct: 194 VDQSKGMLGTFAPQKEPYVYALKEDTTPSGALARGVYSAKLKFEDDDQRCH 346
>AV779190
Length = 539
Score = 30.0 bits (66), Expect = 0.36
Identities = 23/61 (37%), Positives = 28/61 (45%), Gaps = 5/61 (8%)
Frame = +3
Query: 21 EEEHKNKGTNKVGVNYDEAE-----EIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDE 75
EE KNK + V VN DE + EIVP +DE E+ D F K DD+
Sbjct: 288 EESRKNKRYDPVFVN-DELDSEIEDEIVPSDDENED-----DDDDYFGTKRNANAQSDDD 449
Query: 76 S 76
S
Sbjct: 450 S 452
>TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;}, partial (44%)
Length = 700
Score = 29.3 bits (64), Expect = 0.62
Identities = 16/56 (28%), Positives = 33/56 (58%)
Frame = +2
Query: 21 EEEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDES 76
EEE +G G + D+ ++ D+D++EE + E DLG ++ ++ + +D+E+
Sbjct: 128 EEEGGVEGGRGGGGDPDDDDD--DDDDDDEEEEEEEDLGTEYLVRRTVAAAEDEEA 289
>BP034655
Length = 517
Score = 27.7 bits (60), Expect = 1.8
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = +1
Query: 33 GVNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDE 75
G++ D+ EE +E+EEEE + E + + +E E+D+D++
Sbjct: 127 GLSDDDEEEEEEEEEEEEEEEEEEEEEEEEEDEEDDEEDEDED 255
>TC9414 similar to UP|RNPA_SYNPX (Q7U524) Ribonuclease P protein component
(RNaseP protein) (RNase P protein) (Protein C5) ,
partial (10%)
Length = 859
Score = 27.7 bits (60), Expect = 1.8
Identities = 11/21 (52%), Positives = 16/21 (75%)
Frame = -1
Query: 90 VSAVGEKIEPEVKIVSLTIIC 110
V A+G KI+P+ +V LTI+C
Sbjct: 604 VGAIGRKIKPKHSLVILTILC 542
>TC17637
Length = 982
Score = 26.9 bits (58), Expect = 3.1
Identities = 21/63 (33%), Positives = 33/63 (52%), Gaps = 12/63 (19%)
Frame = +3
Query: 39 AEEIVPDEDEEEEPKLELD---LGPQFSLKEQLEKDKDDESLRK---------WKEQLLG 86
A+ I P ++ + +PKLEL+ L S+ + +E D+D E L K KEQ L
Sbjct: 24 AKIIQPRKELDMKPKLELEIQQLKGSLSVLKHIEDDEDAEVLNKVDALHKDLREKEQSLQ 203
Query: 87 NVD 89
++D
Sbjct: 204DID 212
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 26.9 bits (58), Expect = 3.1
Identities = 21/77 (27%), Positives = 40/77 (51%), Gaps = 7/77 (9%)
Frame = +1
Query: 15 SFNAHLEEEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLE----- 69
S +A L + +NK +V + EE+ + +EEEE + E + G + +E+ E
Sbjct: 136 SQSAELPFKLQNKLEAEVQEYEEVEEEVEEEVEEEEEEEEEEEEGEEEEEEEEEEEEVEA 315
Query: 70 --KDKDDESLRKWKEQL 84
+++DDE ++K E +
Sbjct: 316 EAEEEDDEPIQKLLEPM 366
>TC8557 similar to UP|Q9LK52 (Q9LK52) Dbj|BAA90629.1, complete
Length = 1064
Score = 26.9 bits (58), Expect = 3.1
Identities = 13/43 (30%), Positives = 24/43 (55%)
Frame = +3
Query: 37 DEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDESLRK 79
D E+ DE+ +++ E D + EQ++K++ +E LRK
Sbjct: 486 DSDVEVKSDEESDDDDDDEDDTEALLAELEQIKKERAEEKLRK 614
>TC19471
Length = 514
Score = 26.6 bits (57), Expect = 4.0
Identities = 15/42 (35%), Positives = 25/42 (58%)
Frame = +3
Query: 42 IVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDESLRKWKEQ 83
I+ +E + E+PK + Q KE LEK K+ +L+K K++
Sbjct: 216 IIEEEFKCEDPKRNIIEEIQVLSKEALEKGKETAALQKKKKK 341
>TC12700 similar to UP|P93390 (P93390) Phosphate/phosphoenolpyruvate
translocator, partial (9%)
Length = 442
Score = 26.6 bits (57), Expect = 4.0
Identities = 21/78 (26%), Positives = 36/78 (45%)
Frame = -2
Query: 3 AAVGAVSTTKDVSFNAHLEEEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLGPQF 62
+A A+S T++ + +E + +G N G + ++ EDEEEE P
Sbjct: 372 SAEPALSGTEEAALGG---DESEARGLNLKGEEFARCQDRRGKEDEEEEEAPFGFAPPSV 202
Query: 63 SLKEQLEKDKDDESLRKW 80
SL+ E+ + E R+W
Sbjct: 201 SLE---ERRTEKEGRRRW 157
>TC18481 similar to UP|O23166 (O23166) Thiol-disulfide interchange like
protein (Thioredoxin-like protein)
(AT4g37200/C7A10_160), partial (42%)
Length = 602
Score = 26.2 bits (56), Expect = 5.2
Identities = 21/85 (24%), Positives = 42/85 (48%)
Frame = +2
Query: 20 LEEEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDESLRK 79
+E+++K++ N V +N D E+E + ++ P F+ ++ ++ + R
Sbjct: 35 IEQQYKDR-VNFVMLNVDNTNW------EQELDEFGVEGIPHFAFLDKEGNEEGNVVGRL 193
Query: 80 WKEQLLGNVDVSAVGEKIEPEVKIV 104
++ LL NVD A GE P ++V
Sbjct: 194 PRQLLLENVDALARGEASVPHARVV 268
>AV416122
Length = 414
Score = 25.8 bits (55), Expect = 6.8
Identities = 20/70 (28%), Positives = 28/70 (39%)
Frame = +3
Query: 7 AVSTTKDVSFNAHLEEEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKE 66
AVS T D S N+ N V +E E+ D++EE+E
Sbjct: 213 AVSATDDCSSQPKEAANDDNEPDNDVQFEGEEEEDDEEDKEEEDEE-------------- 350
Query: 67 QLEKDKDDES 76
E D+DD+S
Sbjct: 351 --EDDEDDDS 374
>BI420048
Length = 584
Score = 25.8 bits (55), Expect = 6.8
Identities = 10/40 (25%), Positives = 26/40 (65%)
Frame = +2
Query: 35 NYDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDD 74
+YD+ EE++ + E+ + ++E++ + L+EQL + ++
Sbjct: 275 DYDKVEEVLESKYEKLQGQVEMERLERMHLEEQLRQRNEE 394
>CB828220
Length = 533
Score = 25.4 bits (54), Expect = 8.9
Identities = 16/62 (25%), Positives = 27/62 (42%), Gaps = 1/62 (1%)
Frame = +2
Query: 132 TLKEGSQYRLKFSFTVSN-NIVSGLRYTNVVWKTGVRVENTKKMLGTYSPQQEPYTYELE 190
TL EG + SF + N +++T+ +W V + + + P +P YEL
Sbjct: 230 TLYEGGFFNAIMSFPSNYPNSPPSVKFTSEIWHPNVYTDGRVCISILHPPGDDPNGYELA 409
Query: 191 EE 192
E
Sbjct: 410 SE 415
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.132 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,509,493
Number of Sequences: 28460
Number of extensions: 39333
Number of successful extensions: 217
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of query: 235
length of database: 4,897,600
effective HSP length: 87
effective length of query: 148
effective length of database: 2,421,580
effective search space: 358393840
effective search space used: 358393840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144644.6