BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144618.9 + phase: 0
(313 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP036468 135 1e-32
TC9603 similar to GB|AAO63373.1|28950899|BT005309 At2g27770 {Ara... 105 1e-23
BG662442 75 1e-14
TC12602 similar to GB|AAO63373.1|28950899|BT005309 At2g27770 {Ar... 74 3e-14
TC16979 similar to GB|AAM78072.1|21928087|AY125562 AT3g04860/T9J... 61 3e-10
TC11368 similar to GB|AAM51579.1|21436003|AY116945 AT5g11000/T30... 60 6e-10
BF177654 56 7e-09
AV419982 33 0.048
BP066034 31 0.31
AV421186 29 1.2
AV427711 28 2.6
TC15229 weakly similar to UP|O60585 (O60585) Ser/Arg-related nuc... 27 3.4
TC14650 similar to UP|IF3A_TOBAC (Q40554) Eukaryotic translation... 27 4.5
BP044856 27 5.8
TC12083 27 5.8
TC17904 similar to UP|Q8L7V5 (Q8L7V5) AT3g52870/F8J2_40, partial... 27 5.8
BP041967 27 5.8
TC11270 similar to UP|Q9LM88 (Q9LM88) F2D10.15, partial (9%) 26 9.9
BP063262 26 9.9
TC13118 26 9.9
>BP036468
Length = 507
Score = 135 bits (339), Expect = 1e-32
Identities = 67/95 (70%), Positives = 74/95 (77%), Gaps = 1/95 (1%)
Frame = -1
Query: 220 IKNLQWKFRGNQTVMVNKQPVHVFWDVYDWLFSVSGSGPGLFIFKPGPTEVESDKEEKGL 279
+KNLQWKFRGNQTVMVN QPV VFWDV+DWLFS S SGPGLFIFKPGP E ES+KE +
Sbjct: 507 VKNLQWKFRGNQTVMVNMQPVQVFWDVHDWLFSGSASGPGLFIFKPGPPEAESEKEGSAV 328
Query: 280 EG-ESDDGSATSGYYSTKSYTPFESCLVLCAYKLE 313
+G ESDDGS +YST + FE CLVL AYK E
Sbjct: 327 DGCESDDGS----FYSTLNVATFEFCLVLYAYKTE 235
>TC9603 similar to GB|AAO63373.1|28950899|BT005309 At2g27770 {Arabidopsis
thaliana;}, partial (23%)
Length = 844
Score = 105 bits (262), Expect = 1e-23
Identities = 56/160 (35%), Positives = 92/160 (57%), Gaps = 5/160 (3%)
Frame = +1
Query: 110 VYWDLRSAKFAGNCPEPCSDYYVMLVSDEEVVLLLGDYKKKAY-KRMKMRQALVEATLLV 168
++WDL +A + PEP +YV+++ D E+ L+LGD + K+ K+ + + +LL
Sbjct: 1 IFWDLSNANYDSG-PEPVDGFYVLILVDSEIGLVLGDMAGETVSKKFKINSPVAKVSLLS 177
Query: 169 KRENVFAKKSFSTKAKFDEKRKESDIV----VESSTAGNKDPEMWISIDGIVLIHIKNLQ 224
+RE+ ++TKA+F + D++ VE S K P + + ID +I +K LQ
Sbjct: 178 RREHCSGNSLYTTKAQFCDSGTWHDVLIRCSVEESEGLFKSPVLSVCIDQKTVIRVKRLQ 357
Query: 225 WKFRGNQTVMVNKQPVHVFWDVYDWLFSVSGSGPGLFIFK 264
W FRGNQT+ V+ V + WDV+DW F+ SG +F+F+
Sbjct: 358 WNFRGNQTIFVDGLLVDLLWDVHDWFFN-PASGDAVFMFR 474
Score = 26.6 bits (57), Expect = 5.8
Identities = 9/22 (40%), Positives = 15/22 (67%)
Frame = +1
Query: 93 WNRKGYRSFDVDGNQIDVYWDL 114
WN +G ++ VDG +D+ WD+
Sbjct: 358 WNFRGNQTIFVDGLLVDLLWDV 423
>BG662442
Length = 465
Score = 75.1 bits (183), Expect = 1e-14
Identities = 42/128 (32%), Positives = 79/128 (60%), Gaps = 4/128 (3%)
Frame = +2
Query: 106 NQIDVYWDLRSAKFAGNCPEPCSDYYVMLVSDEEVVLLLGDYKKKAY-KRMKMRQALVEA 164
++++V+WDL +AK+ PEP +++ ++ D E+ L+LG+ ++ K++K R L ++
Sbjct: 47 SRVEVFWDLSNAKYDTG-PEPVDGFFIAILVDSELGLVLGEISEETVTKKLKNRTILGKS 223
Query: 165 TLLVKRENVFAKKS-FSTKAKFDEKRKESDIVVESS--TAGNKDPEMWISIDGIVLIHIK 221
+LL +RE+ + ++TKA+F + +I + S + G K P + +SID ++I +K
Sbjct: 224 SLLSRREHCSGNTTLYNTKAQFCDSGTWHEISIRCSGESEGLKSPVLSVSIDKKMVIRVK 403
Query: 222 NLQWKFRG 229
LQW FRG
Sbjct: 404 RLQWNFRG 427
>TC12602 similar to GB|AAO63373.1|28950899|BT005309 At2g27770 {Arabidopsis
thaliana;}, partial (14%)
Length = 728
Score = 73.9 bits (180), Expect = 3e-14
Identities = 39/139 (28%), Positives = 75/139 (53%), Gaps = 3/139 (2%)
Frame = +1
Query: 129 DYYVMLVSDEEVVLLLGDYKKKAYKRMKMRQALVEATLLVKRENVFAKKSFSTKAKFDEK 188
++YV+++ D E+ L LGD + + + + +++ + E ++T+AKF E
Sbjct: 7 EFYVVVLVDSELGLRLGDKDDEIDETLDLSIMKGNFSMVSRSERFSGTTVYATRAKFSES 186
Query: 189 RKESDIVVE--SSTAGNK-DPEMWISIDGIVLIHIKNLQWKFRGNQTVMVNKQPVHVFWD 245
DI++ + G K + + + +D + +K L+W FRGNQT+ V+ V + WD
Sbjct: 187 GDFHDILIRCVAEEEGLKMNHVLSVCLDKKTVFQVKRLRWNFRGNQTIFVDGLVVDMMWD 366
Query: 246 VYDWLFSVSGSGPGLFIFK 264
V+DWLF+ + +F+F+
Sbjct: 367 VHDWLFNPNSDSSAVFLFR 423
Score = 27.7 bits (60), Expect = 2.6
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +1
Query: 93 WNRKGYRSFDVDGNQIDVYWDLRSAKFAGN 122
WN +G ++ VDG +D+ WD+ F N
Sbjct: 304 WNFRGNQTIFVDGLVVDMMWDVHDWLFNPN 393
>TC16979 similar to GB|AAM78072.1|21928087|AY125562 AT3g04860/T9J14_19
{Arabidopsis thaliana;}, partial (26%)
Length = 668
Score = 60.8 bits (146), Expect = 3e-10
Identities = 27/60 (45%), Positives = 41/60 (68%)
Frame = +1
Query: 205 DPEMWISIDGIVLIHIKNLQWKFRGNQTVMVNKQPVHVFWDVYDWLFSVSGSGPGLFIFK 264
DP + I ID ++ +K L WKFRGN T++V+ PV VFWD+++WLF + G +F+F+
Sbjct: 4 DPCLVIRIDSRTVMPVKPLHWKFRGNHTLLVDGIPVEVFWDIHNWLFGYT-IGNAVFMFR 180
Score = 26.9 bits (58), Expect = 4.5
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +1
Query: 87 VKPWCFWNRKGYRSFDVDGNQIDVYWDLRSAKF 119
VKP W +G + VDG ++V+WD+ + F
Sbjct: 49 VKP-LHWKFRGNHTLLVDGIPVEVFWDIHNWLF 144
>TC11368 similar to GB|AAM51579.1|21436003|AY116945 AT5g11000/T30N20_270
{Arabidopsis thaliana;}, partial (14%)
Length = 764
Score = 59.7 bits (143), Expect = 6e-10
Identities = 28/65 (43%), Positives = 43/65 (66%), Gaps = 1/65 (1%)
Frame = +2
Query: 217 LIHIKNLQWKFRGNQTVMVNKQPVHVFWDVYDWLFSVSGS-GPGLFIFKPGPTEVESDKE 275
++ IK L+WKFRGN+ V V+ P+ + WDVY+WLF S G +F+FK E E +++
Sbjct: 5 VMQIKRLKWKFRGNERVEVDGVPIQISWDVYNWLFQKDNSDGHAIFMFK-FEEEEEDEED 181
Query: 276 EKGLE 280
++G E
Sbjct: 182 QRGKE 196
>BF177654
Length = 270
Score = 56.2 bits (134), Expect = 7e-09
Identities = 28/71 (39%), Positives = 43/71 (60%)
Frame = +2
Query: 32 TKTAQSTVTCLYQTSIGGQWRNISVLWCKNLINHTLNLKVDSARGEQFHQNCKIEVKPWC 91
+KTAQ+ V C+YQ I G+ I++ W K+L+ L + +D A + CK++VKPW
Sbjct: 65 SKTAQNLVICVYQCRIRGRSCLITITWSKSLMGLGLTVGIDYASNQCL---CKVDVKPWV 235
Query: 92 FWNRKGYRSFD 102
F RKG +S +
Sbjct: 236 FSRRKGSKSLE 268
>AV419982
Length = 415
Score = 33.5 bits (75), Expect = 0.048
Identities = 21/95 (22%), Positives = 45/95 (47%), Gaps = 9/95 (9%)
Frame = +3
Query: 29 PLPTKTAQSTVTCLYQTSIGGQWRNISVLWCKNLINHTLNLKV-----DS----ARGEQF 79
P P T + T LY T IG +S+ W ++++ +L++ + DS +
Sbjct: 150 PSPPSTNPNLSTYLYNTDIGP----VSLTWSRSIMGRSLHVHLHHHPFDSPLPLSSSSSS 317
Query: 80 HQNCKIEVKPWCFWNRKGYRSFDVDGNQIDVYWDL 114
+ ++ +KP+ FW + G + + + ++W+L
Sbjct: 318 SSSFRLHIKPFIFWKKHGTKKLSPNTH---LFWNL 413
>BP066034
Length = 557
Score = 30.8 bits (68), Expect = 0.31
Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 9/94 (9%)
Frame = -1
Query: 229 GNQTVMVNKQPVHVFWDVYDWLFSVSGSGPGLFIFKPG--PTEVES----DKEEKGLEGE 282
GN+ ++ + + + WDV + G G L + G TE++S D E+ LE +
Sbjct: 335 GNRLLIAFQDGLLILWDVSEAKIVFVGGGKDLXLKDEGGDSTEIDSNLPADILEQNLEDK 156
Query: 283 SDDGSATSGY--YSTKSYT-PFESCLVLCAYKLE 313
+GS + Y +S KS SCL + KL+
Sbjct: 155 EKEGSPSLSYVVHSHKSIVIVLGSCLSMVVMKLD 54
>AV421186
Length = 423
Score = 28.9 bits (63), Expect = 1.2
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = -1
Query: 252 SVSGSGPGLFIFKPGPTEVESDKEEKGLEGESDDGSATSG 291
S +G+G G PG EVES EE + G D+G A G
Sbjct: 231 SEAGAGSGDGAGSPGIVEVESVAEESAVRG-GDEGGAVGG 115
>AV427711
Length = 251
Score = 27.7 bits (60), Expect = 2.6
Identities = 11/29 (37%), Positives = 17/29 (57%)
Frame = -3
Query: 66 TLNLKVDSARGEQFHQNCKIEVKPWCFWN 94
TL+L D G+ FH++ +E + W WN
Sbjct: 108 TLHL*GDGLAGQSFHKDLHLETRIWENWN 22
>TC15229 weakly similar to UP|O60585 (O60585) Ser/Arg-related nuclear matrix
protein, partial (4%)
Length = 965
Score = 27.3 bits (59), Expect = 3.4
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -2
Query: 275 EEKGLEGESDDGSATSGYYSTKSYTPFESC 304
++K EGE+ G+ TS ++ S P ESC
Sbjct: 733 KKKKREGETQKGTTTSWLHAKSSEFPMESC 644
>TC14650 similar to UP|IF3A_TOBAC (Q40554) Eukaryotic translation
initiation factor 3 subunit 10 (eIF-3 theta)
(Eukaryotic translation initiation factor 3 large
subunit) (eIF3a) (PNLA-35), partial (11%)
Length = 978
Score = 26.9 bits (58), Expect = 4.5
Identities = 8/22 (36%), Positives = 15/22 (67%)
Frame = +1
Query: 72 DSARGEQFHQNCKIEVKPWCFW 93
+S +G ++ ++CK +KPW W
Sbjct: 7 NSVKGRKWKRSCKNLLKPWIIW 72
>BP044856
Length = 545
Score = 26.6 bits (57), Expect = 5.8
Identities = 12/24 (50%), Positives = 16/24 (66%), Gaps = 1/24 (4%)
Frame = +2
Query: 71 VDSARGEQFHQNC-KIEVKPWCFW 93
+DSAR QF NC ++E K W F+
Sbjct: 449 LDSARDFQFKPNCQQLEAKKWYFY 520
>TC12083
Length = 700
Score = 26.6 bits (57), Expect = 5.8
Identities = 12/36 (33%), Positives = 18/36 (49%)
Frame = -1
Query: 60 KNLINHTLNLKVDSARGEQFHQNCKIEVKPWCFWNR 95
K +N N+ V Q HQNC++ ++ W NR
Sbjct: 478 KKTVNQLTNIDV------QIHQNCRVLIQGWQSGNR 389
>TC17904 similar to UP|Q8L7V5 (Q8L7V5) AT3g52870/F8J2_40, partial (19%)
Length = 553
Score = 26.6 bits (57), Expect = 5.8
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = -2
Query: 14 KQNLASLMSQRITEEPLPTKTAQSTVTCLYQTSIGGQWRNISVLW 58
K N +L S+ + PLPT A S ++ G+ +N VLW
Sbjct: 528 KANFCALASK---DNPLPTLEALSLTRVHLDAALSGKLKNEMVLW 403
>BP041967
Length = 469
Score = 26.6 bits (57), Expect = 5.8
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -3
Query: 8 RSQSFSKQNLASLMSQRITEEPLPTKTAQS 37
+ Q KQN S +TE PL TKT Q+
Sbjct: 383 QQQKIQKQNSNKRASVLVTEAPLNTKTTQT 294
>TC11270 similar to UP|Q9LM88 (Q9LM88) F2D10.15, partial (9%)
Length = 735
Score = 25.8 bits (55), Expect = 9.9
Identities = 12/44 (27%), Positives = 26/44 (58%)
Frame = +3
Query: 186 DEKRKESDIVVESSTAGNKDPEMWISIDGIVLIHIKNLQWKFRG 229
+E+ +E ++VVE GN+D + +G+V++ +K + +G
Sbjct: 450 EEEEEEEEVVVEDEQ-GNEDEDEDEEEEGVVVVEVKGRKVDSKG 578
>BP063262
Length = 491
Score = 25.8 bits (55), Expect = 9.9
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = -2
Query: 283 SDDGSATSGYYSTKSYTPFES 303
+DDG G+ S+KS +PF+S
Sbjct: 316 ADDGLTQHGFRSSKSVSPFQS 254
>TC13118
Length = 496
Score = 25.8 bits (55), Expect = 9.9
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -2
Query: 79 FHQNCKIEVKPWCFWNRKGYRSFDVDGNQIDVYWDLRSAKFAGNCPEP 126
FH+ +I + N G+R QI V+ L F GN EP
Sbjct: 144 FHEQRRIRARRLWRRNPHGHRLASSSEEQIQVFTILVPMNFTGNSSEP 1
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,904,098
Number of Sequences: 28460
Number of extensions: 86760
Number of successful extensions: 456
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 447
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 452
length of query: 313
length of database: 4,897,600
effective HSP length: 90
effective length of query: 223
effective length of database: 2,336,200
effective search space: 520972600
effective search space used: 520972600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC144618.9