BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144618.8 + phase: 0 /pseudo
(558 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV413363 66 2e-11
TC13142 similar to UP|Q9LTZ1 (Q9LTZ1) Emb|CAB40985.1, partial (11%) 51 3e-11
TC10808 32 0.35
TC8273 UP|BAD11134 (BAD11134) Glutamate-rich protein, complete 27 0.74
TC17225 weakly similar to UP|Q9LJ72 (Q9LJ72) Genomic DNA, chromo... 29 1.8
AV416351 29 2.3
TC11463 homologue to UP|PRO2_TRYBB (P14044) Duplicate procyclin,... 28 3.9
TC13354 similar to UP|Q9SHX4 (Q9SHX4) F1E22.18, partial (43%) 28 3.9
AV770807 28 5.1
TC11995 28 5.1
AV425247 28 5.1
TC18026 similar to UP|AAR87415 (AAR87415) Surface antigen (Fragm... 27 6.7
TC15096 similar to UP|Q9ZUG9 (Q9ZUG9) At2g24260 protein, partial... 27 6.7
TC11721 27 6.7
TC8366 weakly similar to UP|O24088 (O24088) MtN24 protein, parti... 27 6.7
TC12254 similar to UP|Q94JV6 (Q94JV6) At1g35190/T32G9_27, partia... 27 8.7
TC11371 similar to UP|Q8S9K1 (Q8S9K1) At1g23880/T23E23_8, partia... 27 8.7
TC14927 weakly similar to GB|BAC16200.1|22831356|AP005486 transp... 25 9.0
>AV413363
Length = 420
Score = 65.9 bits (159), Expect = 2e-11
Identities = 43/126 (34%), Positives = 67/126 (53%)
Frame = +3
Query: 309 GSCLPRPIYRKNVKHAVERKKLEGIDHNLKNRRDSSQNSKHTLQQSRSMKWKIQQYKPVQ 368
G+C PRP YRK V + +L I S +S+ ++ S++ I + K ++
Sbjct: 3 GTCFPRPQYRKKVDSSQRTSRLNSI---------SGGSSRKAPSRTSSVRNNIAELKSLE 155
Query: 369 VWDWFFKSCEVNGRIVLRDGLISVKEIEECIFKGNCKKLSIKLPAWSLLQCLLTSAKSNS 428
+ D F C G ++ +GLI+ K+IEE +SI L A+ LLQ LL SAK++S
Sbjct: 156 LLDCLFSECFRVGGKMISEGLITGKDIEEAGSSKGSSVISIGLLAYCLLQGLLRSAKASS 335
Query: 429 DGLVIS 434
G++IS
Sbjct: 336 MGILIS 353
>TC13142 similar to UP|Q9LTZ1 (Q9LTZ1) Emb|CAB40985.1, partial (11%)
Length = 609
Score = 51.2 bits (121), Expect(2) = 3e-11
Identities = 28/73 (38%), Positives = 41/73 (55%)
Frame = +2
Query: 445 PKDRVFEWFIGPLLIMKEQLKNLELEESEETCLKELVMRSKNDIPEDWDSTGFPSKDNVR 504
P + FEWF+ PLLI+KEQ+K L SEE L +LV+ + + G + V+
Sbjct: 5 PSEIFFEWFLNPLLIIKEQIKAENLSASEEDYLCKLVLLGGDSERLKSSTIGPAPESEVK 184
Query: 505 RAQLQAIIRRLQG 517
RA+L A+ R +G
Sbjct: 185 RAELDALARSCEG 223
Score = 33.5 bits (75), Expect(2) = 3e-11
Identities = 14/26 (53%), Positives = 21/26 (79%)
Frame = +1
Query: 514 RLQGIGSSMSRMPTFRRKFRNLVKIL 539
+L+GI SM+R PTF+R+F +LV+ L
Sbjct: 211 KLRGITKSMTRFPTFKRRFDDLVRTL 288
>TC10808
Length = 548
Score = 31.6 bits (70), Expect = 0.35
Identities = 20/76 (26%), Positives = 37/76 (48%), Gaps = 18/76 (23%)
Frame = +1
Query: 23 GALIGPIAFAIMVVGNSAVI---------IGLW-------TAHVFWTYYCVAR--LYFLS 64
G LIG + F +++VG VI +G + +A++F+ + +R F
Sbjct: 226 GELIGYLLFLLLIVGGGFVI*QHGYLT*IVGYYFFFPFG*SANIFYVSFLASRSDYCFFF 405
Query: 65 KFFVICFICSNSISLI 80
K +++CFIC + + I
Sbjct: 406 KCYIVCFICKHLLDNI 453
>TC8273 UP|BAD11134 (BAD11134) Glutamate-rich protein, complete
Length = 923
Score = 26.6 bits (57), Expect(2) = 0.74
Identities = 19/58 (32%), Positives = 25/58 (42%)
Frame = -3
Query: 54 YYCVARLYFLSKFFVICFICSNSISLINCWPLKTEQRGLDWFSRLRR*YVCRFLCYFC 111
+ C A L L C CS+ SL+N W L R WF ++C L +FC
Sbjct: 483 FSCFALLR*LLFLHWCCLSCSSFFSLLNYWFLFHFFRFYLWFLC----FLCLRLGFFC 322
Score = 22.3 bits (46), Expect(2) = 0.74
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
Frame = -2
Query: 131 LFLQLLRLLE-RMFKTNFIIASLMVVGQQFKQAVQWFRMSQTFVFTP 176
LFL LL LL R F ++ +V+GQ+ ++ V + F F P
Sbjct: 259 LFLLLLPLLVLRWFLPLGVLVQWLVLGQEPRRLVVLWLFPGMFQFVP 119
>TC17225 weakly similar to UP|Q9LJ72 (Q9LJ72) Genomic DNA, chromosome 3, TAC
clone:K13E13, partial (91%)
Length = 995
Score = 29.3 bits (64), Expect = 1.8
Identities = 20/56 (35%), Positives = 26/56 (45%), Gaps = 1/56 (1%)
Frame = +3
Query: 343 SSQNSKHTLQQSRSMKW-KIQQYKPVQVWDWFFKSCEVNGRIVLRDGLISVKEIEE 397
SS NS T + S+ W K+Q+Y P Q W F S G D + E+EE
Sbjct: 414 SSANSNGTDENCESLGWKKLQEYDPSQK-HWKFLSDLAEGNATREDIISEEDELEE 578
>AV416351
Length = 428
Score = 28.9 bits (63), Expect = 2.3
Identities = 18/67 (26%), Positives = 33/67 (48%), Gaps = 3/67 (4%)
Frame = +3
Query: 438 ELTRMNGPKDRVFEWFIGPLLIMKEQLKNLELEESEETCLKELVMRSKNDIPEDW---DS 494
+L+ MN PK ++ PL + QLK E +E K ++++ D P+ W +
Sbjct: 219 DLSPMNSPKSKLSARPPSPLTLPPSQLKTEEPNVPDEAESKAVLVQKTIDKPKAWHHNNG 398
Query: 495 TGFPSKD 501
+G P ++
Sbjct: 399 SGEPHRE 419
>TC11463 homologue to UP|PRO2_TRYBB (P14044) Duplicate procyclin, partial
(15%)
Length = 517
Score = 28.1 bits (61), Expect = 3.9
Identities = 28/82 (34%), Positives = 37/82 (44%), Gaps = 6/82 (7%)
Frame = +2
Query: 195 LISTFDVVLIT-SIAIWKSPYMLFRGWKRLLED---LVGRRG--PFLETECVPFAGLAII 248
LI FD T S+ SP F + R L L +RG P T V AG
Sbjct: 56 LIPQFDSARFTISVPATLSPLFFF--FLRFLSSSSFLCSQRGNKPERTTMEVEGAGAGAA 229
Query: 249 LWPLAVLGAVLAAIIVSFFLSL 270
LW AVLG +L ++ S +++L
Sbjct: 230 LWQGAVLGGILFWVVFSSYINL 295
>TC13354 similar to UP|Q9SHX4 (Q9SHX4) F1E22.18, partial (43%)
Length = 689
Score = 28.1 bits (61), Expect = 3.9
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 68 VICFICSNSISLINCWPLKTEQRGLDWFSRLRR* 101
VICF+ S I N W LK+E +++R R+*
Sbjct: 33 VICFLTSMVIKFRNRWSLKSETLSRKFWTRQRK* 134
>AV770807
Length = 490
Score = 27.7 bits (60), Expect = 5.1
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -1
Query: 230 RRGPFLETECVPFAGLAIILWPLAVLGAVL 259
RRGP +E +C+ L ++ W L A+L
Sbjct: 280 RRGPSIEEDCIVLDQLKVVCWVLFFCFAIL 191
>TC11995
Length = 726
Score = 27.7 bits (60), Expect = 5.1
Identities = 16/37 (43%), Positives = 19/37 (51%)
Frame = -3
Query: 55 YCVARLYFLSKFFVICFICSNSISLINCWPLKTEQRG 91
+CVA Y L+ C ICSN + N W L TE G
Sbjct: 253 FCVAFGYILTHL-TRCSICSNYRNASNIWTL*TETDG 146
>AV425247
Length = 417
Score = 27.7 bits (60), Expect = 5.1
Identities = 14/31 (45%), Positives = 22/31 (70%)
Frame = -3
Query: 42 IIGLWTAHVFWTYYCVARLYFLSKFFVICFI 72
+I + T+HVF+ YYC + LYF + F + CF+
Sbjct: 106 VIKVATSHVFFRYYC-SGLYFFA-FRL*CFL 20
>TC18026 similar to UP|AAR87415 (AAR87415) Surface antigen (Fragment),
partial (13%)
Length = 522
Score = 27.3 bits (59), Expect = 6.7
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 4/44 (9%)
Frame = +2
Query: 37 GNSAVIIGLWTAHVFWTYYCVARLYFLSKFFV----ICFICSNS 76
G S +I WTA + T+Y V+ +FL+ F C +C +S
Sbjct: 38 GESPCLIQWWTAKLKSTFYLVSFFHFLACFVQASPNFCGLCPSS 169
>TC15096 similar to UP|Q9ZUG9 (Q9ZUG9) At2g24260 protein, partial (17%)
Length = 670
Score = 27.3 bits (59), Expect = 6.7
Identities = 11/34 (32%), Positives = 17/34 (49%)
Frame = -1
Query: 223 LLEDLVGRRGPFLETECVPFAGLAIILWPLAVLG 256
L D+ G GP + + GL ++LWP + G
Sbjct: 295 LFADMPGEEGPLSPLDPITGLGLLLMLWPAFIKG 194
>TC11721
Length = 327
Score = 27.3 bits (59), Expect = 6.7
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +1
Query: 62 FLSKFFVICFICSNSISLINCWPLK 86
F + FF+ CF CS S++ I W K
Sbjct: 34 FFTFFFITCFYCSLSLTWIFLWCCK 108
>TC8366 weakly similar to UP|O24088 (O24088) MtN24 protein, partial (28%)
Length = 612
Score = 27.3 bits (59), Expect = 6.7
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = -1
Query: 42 IIGLWTAHVFWTYYCVARLYFLSKFFVICFICS 74
II T F + + L+FL FF C +CS
Sbjct: 234 IIAATTIPTFCARFRIRVLFFLQAFFYFCLLCS 136
>TC12254 similar to UP|Q94JV6 (Q94JV6) At1g35190/T32G9_27, partial (34%)
Length = 486
Score = 26.9 bits (58), Expect = 8.7
Identities = 11/29 (37%), Positives = 18/29 (61%)
Frame = +1
Query: 446 KDRVFEWFIGPLLIMKEQLKNLELEESEE 474
KD + W I P+L++ E+L + +SEE
Sbjct: 136 KDFIMVWSIAPMLVIPEKLHLFIMRKSEE 222
>TC11371 similar to UP|Q8S9K1 (Q8S9K1) At1g23880/T23E23_8, partial (41%)
Length = 1023
Score = 26.9 bits (58), Expect = 8.7
Identities = 15/63 (23%), Positives = 32/63 (49%)
Frame = +2
Query: 188 *TLKKSHLISTFDVVLITSIAIWKSPYMLFRGWKRLLEDLVGRRGPFLETECVPFAGLAI 247
*T ++ L+ T ++ ++ + IW+ ++ +G +RLLE+ G L + L
Sbjct: 536 *TSQRE*LLMTEEIFMLQIL*IWQLGKLVIQGSQRLLEENGAVEGAMLMDQVKKLNFLMT 715
Query: 248 ILW 250
++W
Sbjct: 716 LMW 724
>TC14927 weakly similar to GB|BAC16200.1|22831356|AP005486 transposase-like
{Oryza sativa (japonica cultivar-group);} , partial
(11%)
Length = 539
Score = 24.6 bits (52), Expect(2) = 9.0
Identities = 17/45 (37%), Positives = 24/45 (52%)
Frame = +3
Query: 102 YVCRFLCYFCR*LVLLVAF*VELDMAFLLLFLQLLRLLERMFKTN 146
++ FL YFC VLL L+ LF+ LL L+E + +TN
Sbjct: 411 FLMLFLVYFCSCFVLLCVL-AHLEEPCFQLFI-LLILIE*LIRTN 539
Score = 20.4 bits (41), Expect(2) = 9.0
Identities = 8/24 (33%), Positives = 14/24 (58%)
Frame = +2
Query: 59 RLYFLSKFFVICFICSNSISLINC 82
+L LS FV+ IC + +++C
Sbjct: 359 QLSLLSTLFVVSVICFSISYVVSC 430
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.333 0.144 0.458
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,565,723
Number of Sequences: 28460
Number of extensions: 164370
Number of successful extensions: 1179
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 1167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1176
length of query: 558
length of database: 4,897,600
effective HSP length: 95
effective length of query: 463
effective length of database: 2,193,900
effective search space: 1015775700
effective search space used: 1015775700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144618.8