BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144591.1 - phase: 0
(167 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10830 weakly similar to UP|Q94JL9 (Q94JL9) At1g68400/T2E12_5, ... 35 0.007
TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fra... 33 0.025
CN825556 33 0.033
TC9268 similar to UP|Q9LHE6 (Q9LHE6) Genomic DNA, chromosome 3, ... 32 0.055
TC14193 similar to UP|Q8S2S7 (Q8S2S7) Ethylene responsive elemen... 30 0.16
TC15704 similar to UP|O22265 (O22265) Expressed protein (At2g474... 30 0.28
CB827201 29 0.36
TC19044 similar to UP|Q9LJH9 (Q9LJH9) Gb|AAD25781.1, partial (16%) 29 0.47
BP062816 28 0.61
TC8445 similar to UP|O80910 (O80910) At2g38410 protein, partial ... 28 0.61
TC10343 similar to UP|Q9SZZ1 (Q9SZZ1) Fibrillarin-like protein (... 28 0.61
TC12132 similar to UP|AAR14275 (AAR14275) Predicted protein, par... 28 0.61
TC11992 similar to UP|Q9QY28 (Q9QY28) Espin (Fragment), partial ... 28 1.0
TC11373 similar to UP|Q944G2 (Q944G2) Mevalonate kinase, partial... 28 1.0
TC15341 similar to UP|Q9M0H8 (Q9M0H8) Predicted proline-rich pro... 28 1.0
BP056955 28 1.0
TC10660 similar to UP|Q8H5M9 (Q8H5M9) OJ1047_A06.31 protein, par... 27 1.4
TC11087 weakly similar to UP|Q8GS92 (Q8GS92) OJ1513_F02.5 protei... 27 1.8
TC12260 27 2.3
AU088929 25 5.2
>TC10830 weakly similar to UP|Q94JL9 (Q94JL9) At1g68400/T2E12_5, partial
(24%)
Length = 702
Score = 35.0 bits (79), Expect = 0.007
Identities = 29/109 (26%), Positives = 44/109 (39%), Gaps = 1/109 (0%)
Frame = +1
Query: 6 LSAHMQTLAQTHKMDQLEQELHELRKEMTAL-RAKVEELTNRVSSLMVTKDLPQFQQRPQ 64
L +H + L QTH M+ LH R+ + R +R L +LP PQ
Sbjct: 226 LQSHFRHLQQTHHMELHHPPLHLARRHLPPKPRLPPRPRKSRPPRLPPPLNLPHSAPCPQ 405
Query: 65 PPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLPSLLKGNYVQTRPPPPRP 113
P +QP + P P+P+ + L L + ++ P PP P
Sbjct: 406 PQKQPLPRPHTKPLQPHRHETPLPLP-QQLLR*LPRHAHLPHPPLPP*P 549
>TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fragment),
partial (6%)
Length = 806
Score = 33.1 bits (74), Expect = 0.025
Identities = 19/65 (29%), Positives = 30/65 (45%), Gaps = 4/65 (6%)
Frame = +2
Query: 54 KDLPQFQQ----RPQPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLPSLLKGNYVQTRPP 109
+ LPQ QQ + QPP+ QQ +QQ P+ + + + LP L + Q +P
Sbjct: 2 QQLPQLQQQQQLQQQPPQLTQLQQQQQQQLPQVQQQQLSQMQQQQLPQLQQQQLSQLQPQ 181
Query: 110 PPRPE 114
P+
Sbjct: 182 QQLPQ 196
>CN825556
Length = 655
Score = 32.7 bits (73), Expect = 0.033
Identities = 22/63 (34%), Positives = 30/63 (46%), Gaps = 1/63 (1%)
Frame = +1
Query: 56 LPQFQQRPQPPRQPSFTQQPR-QQAPRTRFDPIPMKYAEFLPSLLKGNYVQTRPPPPRPE 114
LPQ Q +PQP QP QP+ Q P+ + P+P P+ L + P P PE
Sbjct: 460 LPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPVPP------PASLPQPLLAPLPSPGLPE 621
Query: 115 KLP 117
+ P
Sbjct: 622 QKP 630
Score = 26.6 bits (57), Expect = 2.3
Identities = 23/69 (33%), Positives = 28/69 (40%)
Frame = +1
Query: 56 LPQFQQRPQPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLPSLLKGNYVQTRPPPPRPEK 115
LP Q P PP P QP+ Q P+ + P P + P Q +P PP P
Sbjct: 424 LPPPQPVPPPPSLPQPQPQPQPQ-PQPQPQPQPQPQPQPQP--------QPQPVPP-PAS 573
Query: 116 LPAGFRADL 124
LP A L
Sbjct: 574 LPQPLLAPL 600
>TC9268 similar to UP|Q9LHE6 (Q9LHE6) Genomic DNA, chromosome 3, P1 clone:
MZE19, partial (30%)
Length = 805
Score = 32.0 bits (71), Expect = 0.055
Identities = 19/63 (30%), Positives = 26/63 (41%), Gaps = 3/63 (4%)
Frame = +1
Query: 60 QQRPQPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLPS---LLKGNYVQTRPPPPRPEKL 116
Q P PPR P + R P+P++ + L L N+ + RPPP RP
Sbjct: 325 QLPPTPPRHPPHPPPLESPSLRLPLRPLPLRLLQLLRQPRHLRPFNFPRHRPPPRRPPSR 504
Query: 117 PAG 119
G
Sbjct: 505 ALG 513
>TC14193 similar to UP|Q8S2S7 (Q8S2S7) Ethylene responsive element binding
factor 4-like protein, partial (50%)
Length = 1128
Score = 30.4 bits (67), Expect = 0.16
Identities = 22/63 (34%), Positives = 33/63 (51%), Gaps = 1/63 (1%)
Frame = +1
Query: 57 PQFQQRPQPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFL-PSLLKGNYVQTRPPPPRPEK 115
P ++R PP QP Q P APR R+ +P+ F+ + ++G+ T PPR E
Sbjct: 64 PPLRRRETPPPQPPPPQLP-P*APRARYTSLPIMQPRFISEAFVRGHGAVT---PPRSE- 228
Query: 116 LPA 118
+PA
Sbjct: 229 IPA 237
>TC15704 similar to UP|O22265 (O22265) Expressed protein
(At2g47450/T30B22.25), partial (25%)
Length = 664
Score = 29.6 bits (65), Expect = 0.28
Identities = 20/61 (32%), Positives = 23/61 (36%)
Frame = -1
Query: 57 PQFQQRPQPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLPSLLKGNYVQTRPPPPRPEKL 116
P + P PPR PS P+ TRF P A P+ PPPPR
Sbjct: 262 PPPPRTPAPPRNPSPDPPPQTSHSPTRFHHRP-STARDTPNSHPSPSAPISPPPPRTRTP 86
Query: 117 P 117
P
Sbjct: 85 P 83
>CB827201
Length = 374
Score = 29.3 bits (64), Expect = 0.36
Identities = 25/98 (25%), Positives = 32/98 (32%), Gaps = 6/98 (6%)
Frame = +1
Query: 57 PQFQQRPQPPRQPSFTQQPRQQAPRTRFDPIP------MKYAEFLPSLLKGNYVQTRPPP 110
P + PP P R P T P +++ P L ++ QT PP
Sbjct: 16 PSLRHHAPPPFPPHHLPSTRPHKPNTPIRKTPHQFRRRTRHSMHHPPHLPSHFQQTHPPQ 195
Query: 111 PRPEKLPAGFRADLSCVFHQGAPGHDVERCYALRNAVQ 148
P P L R L H R ALR+ Q
Sbjct: 196 PHPPPLLRRLRRRLQT--------HPRHRLLALRH*AQ 285
>TC19044 similar to UP|Q9LJH9 (Q9LJH9) Gb|AAD25781.1, partial (16%)
Length = 620
Score = 28.9 bits (63), Expect = 0.47
Identities = 22/70 (31%), Positives = 27/70 (38%)
Frame = +1
Query: 26 LHELRKEMTALRAKVEELTNRVSSLMVTKDLPQFQQRPQPPRQPSFTQQPRQQAPRTRFD 85
LH L + R +R SL + +PQ P P +PSF P Q R R
Sbjct: 229 LHVLHHHLRRPRQSHLRHPDRHGSLR--RRVPQHPHLPHQPHRPSFPVSPPQHRRRRRST 402
Query: 86 PIPMKYAEFL 95
P P FL
Sbjct: 403 PPPPPLFFFL 432
>BP062816
Length = 510
Score = 28.5 bits (62), Expect = 0.61
Identities = 18/68 (26%), Positives = 28/68 (40%), Gaps = 4/68 (5%)
Frame = -1
Query: 63 PQPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLP---SLLKGNYVQTRPPPPRP-EKLPA 118
PQP + + PR P + D + + P + G+ + PPPP P E
Sbjct: 447 PQPNHRHKHSHHPRSMTPASPSDLVCQL*TQSAPIRRRSVTGSQLPPPPPPPPPSESAAT 268
Query: 119 GFRADLSC 126
+ +LSC
Sbjct: 267 STKEELSC 244
>TC8445 similar to UP|O80910 (O80910) At2g38410 protein, partial (7%)
Length = 841
Score = 28.5 bits (62), Expect = 0.61
Identities = 18/52 (34%), Positives = 24/52 (45%)
Frame = +1
Query: 60 QQRPQPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLPSLLKGNYVQTRPPPP 111
Q +PQP QP QQP+ Q P+ + +F + Y T PPPP
Sbjct: 145 QSQPQPQSQPQHIQQPQPQ-PQLQHIQQSQPQPQFQNQHFQ--YPGTYPPPP 291
Score = 24.6 bits (52), Expect = 8.9
Identities = 17/57 (29%), Positives = 26/57 (44%), Gaps = 1/57 (1%)
Frame = +1
Query: 57 PQFQQRPQPPRQPSFTQQPRQQA-PRTRFDPIPMKYAEFLPSLLKGNYVQTRPPPPR 112
P FQ PQP Q Q QQ+ P+ + P ++ + P L ++Q P P+
Sbjct: 82 PHFQPHPQPQLQSQPQPQHIQQSQPQPQSQPQHIQQPQPQPQL---QHIQQSQPQPQ 243
>TC10343 similar to UP|Q9SZZ1 (Q9SZZ1) Fibrillarin-like protein (Fibrillarin
2) (AtFib2), partial (85%)
Length = 1116
Score = 28.5 bits (62), Expect = 0.61
Identities = 23/62 (37%), Positives = 25/62 (40%)
Frame = -2
Query: 56 LPQFQQRPQPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLPSLLKGNYVQTRPPPPRPEK 115
LP F P PPR P PR P P+P P LKG + PPP P
Sbjct: 236 LPPFIPPPLPPRPP-----PRPPLPPP--PPLPP------PRALKGVPLPPPLPPPSPLP 96
Query: 116 LP 117
LP
Sbjct: 95 LP 90
>TC12132 similar to UP|AAR14275 (AAR14275) Predicted protein, partial (14%)
Length = 698
Score = 28.5 bits (62), Expect = 0.61
Identities = 14/46 (30%), Positives = 20/46 (43%)
Frame = +1
Query: 80 PRTRFDPIPMKYAEFLPSLLKGNYVQTRPPPPRPEKLPAGFRADLS 125
P DP P +++F +L G PPP +P P F +S
Sbjct: 316 PGQGHDPFPSSFSKFNNALTAGLLNPMSPPPDKPRSSPTLFEMMVS 453
>TC11992 similar to UP|Q9QY28 (Q9QY28) Espin (Fragment), partial (4%)
Length = 560
Score = 27.7 bits (60), Expect = 1.0
Identities = 30/124 (24%), Positives = 49/124 (39%), Gaps = 10/124 (8%)
Frame = +2
Query: 13 LAQTHKMDQLEQELHELRKEMTALRAKVEELTNRVSSLMV---------TKDLPQFQQRP 63
+A T ++++ + + + A+ + + +T SS+ T DL F++RP
Sbjct: 35 IAPTTRVEKTKSSSFNPKLGLEAMSPRTQAVTPTNSSIKTLNGNCGSAATLDLHPFRKRP 214
Query: 64 QPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLPSLLKGNYVQTRPPPP-RPEKLPAGFRA 122
+ P+ S P+P PSLL + PPPP RP P A
Sbjct: 215 KIPKDESLEF------------PLP-------PSLLLSSPPPPPPPPPPRPTPSPPSPPA 337
Query: 123 DLSC 126
SC
Sbjct: 338 KESC 349
>TC11373 similar to UP|Q944G2 (Q944G2) Mevalonate kinase, partial (28%)
Length = 572
Score = 27.7 bits (60), Expect = 1.0
Identities = 17/65 (26%), Positives = 25/65 (38%)
Frame = -3
Query: 54 KDLPQFQQRPQPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLPSLLKGNYVQTRPPPPRP 113
++ P P PP +P + I +P G+ V+T+PPPP P
Sbjct: 324 EEFPNLTSTPVPPISAMIHWKPHDSSSVITLSII-------VPDSNVGSNVRTQPPPPAP 166
Query: 114 EKLPA 118
L A
Sbjct: 165 VNLEA 151
>TC15341 similar to UP|Q9M0H8 (Q9M0H8) Predicted proline-rich protein,
partial (33%)
Length = 1002
Score = 27.7 bits (60), Expect = 1.0
Identities = 33/137 (24%), Positives = 56/137 (40%), Gaps = 24/137 (17%)
Frame = +1
Query: 5 NLSAHMQTLAQTHKMDQLEQELHELRKEMTALR------------------------AKV 40
+L H+Q + ++ ++ + +QEL E +KE+ L+ +K
Sbjct: 346 SLEKHLQEVHRSVQILRDKQELAETQKELAKLQLAQKESSSSSHSQSNEDKSPSTTDSKK 525
Query: 41 EELTNRVSSLMVTKDLPQFQQRPQPPRQPSFTQQPRQQAPRTRFDPIPMKYAEFLPSLLK 100
+ + S+ + LP Q PQP QP+ Q QAP P + A ++PS
Sbjct: 526 ADNASDASNQQLALALPN-QIAPQP--QPAAAQ---PQAPAPNVTQAPQQPAYYIPSTPL 687
Query: 101 GNYVQTRPPPPRPEKLP 117
QT P+ + LP
Sbjct: 688 PGNTQTVAQLPQNQYLP 738
>BP056955
Length = 581
Score = 27.7 bits (60), Expect = 1.0
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +2
Query: 57 PQFQQRPQPPRQPSFTQQPRQQ 78
PQ Q +PQP QP QP+QQ
Sbjct: 191 PQPQPQPQPQPQPQSQPQPQQQ 256
Score = 25.8 bits (55), Expect = 4.0
Identities = 15/37 (40%), Positives = 20/37 (53%)
Frame = +2
Query: 42 ELTNRVSSLMVTKDLPQFQQRPQPPRQPSFTQQPRQQ 78
+L VSS+ + PQ Q +PQP QP QP+ Q
Sbjct: 143 DLFTTVSSIY-HRSQPQPQPQPQPQPQPQPQSQPQPQ 250
>TC10660 similar to UP|Q8H5M9 (Q8H5M9) OJ1047_A06.31 protein, partial (12%)
Length = 417
Score = 27.3 bits (59), Expect = 1.4
Identities = 19/64 (29%), Positives = 26/64 (39%), Gaps = 5/64 (7%)
Frame = -3
Query: 56 LPQFQQRPQPPRQPSFTQQPRQQ----APRTRFDP-IPMKYAEFLPSLLKGNYVQTRPPP 110
+P P P PS+ P + APR R P LPS ++ + PPP
Sbjct: 205 VPALPHAPPPLAAPSYAPPPLLRISPPAPRWRTPSHAPPSCPSLLPSPVRQERGPSPPPP 26
Query: 111 PRPE 114
P P+
Sbjct: 25 PPPQ 14
>TC11087 weakly similar to UP|Q8GS92 (Q8GS92) OJ1513_F02.5 protein
(OJ1118_G09.13 protein), partial (6%)
Length = 689
Score = 26.9 bits (58), Expect = 1.8
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +2
Query: 66 PRQPSFTQQPRQQAPRTRFDPIP 88
P+ PS +QPR+ P R P+P
Sbjct: 200 PQHPSLQEQPRRPPPTLRRPPLP 268
>TC12260
Length = 320
Score = 26.6 bits (57), Expect = 2.3
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +3
Query: 60 QQRPQPPRQPSFTQQPRQQAP 80
Q R QPPRQPS TQ +P
Sbjct: 180 QPR*QPPRQPS*TQMAESPSP 242
>AU088929
Length = 720
Score = 25.4 bits (54), Expect = 5.2
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = -3
Query: 108 PPPPRPEKLPAGFRADLSCVFHQGAPGHDVERC 140
PP + P+GF A+ C+ APG + +C
Sbjct: 562 PPRIAAKNKPSGFNANXDCII---APGRSLVQC 473
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.132 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,882,999
Number of Sequences: 28460
Number of extensions: 47309
Number of successful extensions: 519
Number of sequences better than 10.0: 85
Number of HSP's better than 10.0 without gapping: 486
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 505
length of query: 167
length of database: 4,897,600
effective HSP length: 84
effective length of query: 83
effective length of database: 2,506,960
effective search space: 208077680
effective search space used: 208077680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144591.1