Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144564.12 + phase: 0 
         (357 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

CB829575                                                               96  9e-21
TC16854                                                                33  0.096
AV417211                                                               30  0.81
TC15386 similar to UP|Q8W1N7 (Q8W1N7) CSLC7 (Fragment), partial ...    29  1.4
TC11414                                                                29  1.4
TC7929 similar to UP|GGH_ARATH (O65355) Gamma-glutamyl hydrolase...    28  1.8
BP034218                                                               28  1.8
TC14908 weakly similar to PIR|A86218|A86218 protein T27G7.18 [im...    28  1.8
TC13015                                                                28  2.4
AV780410                                                               28  3.1
TC16487 similar to UP|Q9LT09 (Q9LT09) 50S ribosomal protein L24,...    28  3.1
TC16579 weakly similar to UP|AAR82740 (AAR82740) SD09672p (Fragm...    28  3.1
BP066447                                                               27  4.0
AV407648                                                               27  4.0
AV417236                                                               27  5.3
TC14033 similar to GB|AAO63396.1|28950945|BT005332 At5g13570 {Ar...    27  6.9
TC14728 similar to UP|Q9LSL0 (Q9LSL0) Emb|CAB16809.1, partial (86%)    27  6.9
BU494245                                                               26  9.0
TC9029 similar to UP|Q7X9G7 (Q7X9G7) Alpha-L-arabinofuranosidase...    26  9.0

>CB829575 
          Length = 521

 Score = 95.9 bits (237), Expect = 9e-21
 Identities = 55/145 (37%), Positives = 83/145 (56%), Gaps = 6/145 (4%)
 Frame = +3

Query: 213 FSCISNPYMFKQLLELSVEELIAKGNSSLLARRNAASKLLDKVFRVRLGRGFYGECLGVR 272
           F+   NP +F QLL L ++ +I++G  S+  ++      L K + + LG G YG CL V 
Sbjct: 18  FNAQINPSVFDQLLSLDLDSVISQGVVSISHKQKLIDDCLSKSYEIALGNGAYGHCLAVH 197

Query: 273 AD-GNSNLSDEIGMLLSVKSASIGLRPIGAVIFM-----QRNNLKMCLRSSDNATDTSEV 326
           AD   S L  E+G  L+ KS    LR IGAV++          LK+ LRS DN  DT+ +
Sbjct: 198 ADTALSQLRSELGHQLATKSQKPKLRGIGAVVYKVPELENDQKLKISLRSVDN-EDTTPI 374

Query: 327 AKAYGGGGSASSSSFIIRMDEYNQW 351
           ++ +GGGG  ++SSF++  +E+ QW
Sbjct: 375 SQEFGGGGHRNASSFMLMCEEFEQW 449


>TC16854 
          Length = 568

 Score = 32.7 bits (73), Expect = 0.096
 Identities = 17/42 (40%), Positives = 25/42 (59%)
 Frame = -1

Query: 300 GAVIFMQRNNLKMCLRSSDNATDTSEVAKAYGGGGSASSSSF 341
           G V+ + RN L  CL S+  +TD+ + ++A GGG    S SF
Sbjct: 286 GEVVDLLRNIL--CLSSAKQSTDSEQTSRAVGGGEGLKSCSF 167


>AV417211 
          Length = 171

 Score = 29.6 bits (65), Expect = 0.81
 Identities = 13/28 (46%), Positives = 18/28 (63%)
 Frame = +1

Query: 41 VLYNYPSFSGAFSSLFAHLFHTRHNLPS 68
          +LY+YP   G F++L AHLF    +L S
Sbjct: 46 ILYHYPCPDGVFAALAAHLFFKATSLSS 129


>TC15386 similar to UP|Q8W1N7 (Q8W1N7) CSLC7 (Fragment), partial (22%)
          Length = 689

 Score = 28.9 bits (63), Expect = 1.4
 Identities = 20/55 (36%), Positives = 26/55 (46%), Gaps = 2/55 (3%)
 Frame = +3

Query: 70  SLPFSSVPSLAFSCYNIGLKICA--LKAFRHAICLTFFLQTNSLIIGFDHRKSVL 122
           S  FSS+P    SC   GL   A  LK  RH  C   FL   + I+G + R  ++
Sbjct: 168 SFLFSSLPRFVISCDGFGLNWGAGELKLIRHGNCFACFL---TPILGSESRLQLM 323


>TC11414 
          Length = 701

 Score = 28.9 bits (63), Expect = 1.4
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = -1

Query: 204 IGLSEWRSRFSCISNPYMFKQLLEL 228
           I L +WRS+  CI++ Y+FKQ   L
Sbjct: 473 IHLIKWRSQTCCITSNYLFKQYTRL 399


>TC7929 similar to UP|GGH_ARATH (O65355) Gamma-glutamyl hydrolase precursor
            (Gamma-Glu-X carboxypeptidase) (Conjugase) (GH) ,
           partial (83%)
          Length = 1299

 Score = 28.5 bits (62), Expect = 1.8
 Identities = 13/37 (35%), Positives = 21/37 (56%)
 Frame = -1

Query: 10  PPCVEIGYRSLRSDAALEAIAKASEDKVPNIVLYNYP 46
           PPCVE   R ++   +L+AI + + D   N +L + P
Sbjct: 900 PPCVEFPMRPIQRRFSLDAIGRQTLDSYVNALLKHKP 790


>BP034218 
          Length = 516

 Score = 28.5 bits (62), Expect = 1.8
 Identities = 13/37 (35%), Positives = 21/37 (56%)
 Frame = +1

Query: 10  PPCVEIGYRSLRSDAALEAIAKASEDKVPNIVLYNYP 46
           PPCVE   R ++   +L+AI + + D   N +L + P
Sbjct: 217 PPCVEFPMRPIQRRFSLDAIGRQTLDSYVNALLKHKP 327


>TC14908 weakly similar to PIR|A86218|A86218 protein T27G7.18 [imported] -
           Arabidopsis thaliana {Arabidopsis thaliana;}, partial
           (68%)
          Length = 1219

 Score = 28.5 bits (62), Expect = 1.8
 Identities = 17/48 (35%), Positives = 25/48 (51%), Gaps = 3/48 (6%)
 Frame = -3

Query: 58  HLFHTRHNLPSL-SLPFSSVPSLAF--SCYNIGLKICALKAFRHAICL 102
           HL  +  +LPSL  LP S   SL +      IGL +C +  + H +C+
Sbjct: 503 HLKRSSSSLPSLMELPLSQDVSLCWRGQMMTIGLCLCLVHHYSHIVCM 360


>TC13015 
          Length = 528

 Score = 28.1 bits (61), Expect = 2.4
 Identities = 13/32 (40%), Positives = 20/32 (61%)
 Frame = +1

Query: 35  DKVPNIVLYNYPSFSGAFSSLFAHLFHTRHNL 66
           DK+P I++ NYPS  G  S L   ++  RH++
Sbjct: 271 DKMPRILMVNYPS*CG--SELLIEVWQARHHV 360


>AV780410 
          Length = 548

 Score = 27.7 bits (60), Expect = 3.1
 Identities = 24/95 (25%), Positives = 38/95 (39%)
 Frame = +2

Query: 39  NIVLYNYPSFSGAFSSLFAHLFHTRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAFRH 98
           N+V+  Y   S    +L+   FHTR   PS+   ++S+    FS +              
Sbjct: 152 NVVVVVYEMVSRGSHNLYTSRFHTRKLSPSIITFYTSL-IFGFSIF-------------- 286

Query: 99  AICLTFFLQTNSLIIGFDHRKSVLSQIPSTNECPE 133
                FF    + II  D    +LSQ   +++ PE
Sbjct: 287 ----VFFFYVRNTIIDEDQPHPLLSQQSLSHQVPE 379


>TC16487 similar to UP|Q9LT09 (Q9LT09) 50S ribosomal protein L24, complete
          Length = 934

 Score = 27.7 bits (60), Expect = 3.1
 Identities = 15/59 (25%), Positives = 28/59 (47%)
 Frame = -3

Query: 192 RHWSLPDIKPFNIGLSEWRSRFSCISNPYMFKQLLELSVEELIAKGNSSLLARRNAASK 250
           RHW    +   +IG   W   F+C   P+M   LL++ + ++    N ++L   +  +K
Sbjct: 428 RHW----VTNLHIGGMNWG--FNCKDAPFMTLPLLDMLLYQIFTLNNDTILRANDTLNK 270


>TC16579 weakly similar to UP|AAR82740 (AAR82740) SD09672p (Fragment),
           partial (5%)
          Length = 705

 Score = 27.7 bits (60), Expect = 3.1
 Identities = 15/62 (24%), Positives = 26/62 (41%), Gaps = 8/62 (12%)
 Frame = +3

Query: 127 STNECPENIMINLN--------HEKSSSRAVYEYFTDKHEDIKTSNGVVPSLVDSKDKGR 178
           ST+ CP+  +   N        HE    +  ++    +H+     NG+ PS+   KD+  
Sbjct: 474 STDPCPQVTVQETNEQENASEKHEHQCEKQEHQCEKQEHQSDSAQNGIEPSVATDKDEVM 653

Query: 179 VE 180
            E
Sbjct: 654 AE 659


>BP066447 
          Length = 320

 Score = 27.3 bits (59), Expect = 4.0
 Identities = 24/85 (28%), Positives = 33/85 (38%), Gaps = 3/85 (3%)
 Frame = -2

Query: 201 PFNIGLSEWRSRFSCISNPYMFKQLLELSVEELIAKGNSSLLARRNAASKLLDKVFRVRL 260
           PF + +  +RSRF+  + P  F     +SV  L      SL+            VF   L
Sbjct: 301 PFTVSIRRFRSRFAVFNFPQNFWXFQSISVFLLRIDSCFSLIGFWGLVHWWEVSVFGE*L 122

Query: 261 ---GRGFYGECLGVRADGNSNLSDE 282
              G  F G+C G   DG     +E
Sbjct: 121 QFGGVWFLGKCRGFLRDGYKQCGEE 47


>AV407648 
          Length = 430

 Score = 27.3 bits (59), Expect = 4.0
 Identities = 11/24 (45%), Positives = 16/24 (65%)
 Frame = +1

Query: 38 PNIVLYNYPSFSGAFSSLFAHLFH 61
          PN+V  + PSFS + SS  +H F+
Sbjct: 52 PNVVSQSLPSFSSSSSSFLSHKFN 123


>AV417236 
          Length = 399

 Score = 26.9 bits (58), Expect = 5.3
 Identities = 14/21 (66%), Positives = 16/21 (75%)
 Frame = +1

Query: 65  NLPSLSLPFSSVPSLAFSCYN 85
           NLP L LPF  +PSLAFS Y+
Sbjct: 112 NLPLL-LPFQLLPSLAFSFYH 171


>TC14033 similar to GB|AAO63396.1|28950945|BT005332 At5g13570 {Arabidopsis
           thaliana;}, partial (52%)
          Length = 583

 Score = 26.6 bits (57), Expect = 6.9
 Identities = 14/53 (26%), Positives = 25/53 (46%), Gaps = 5/53 (9%)
 Frame = -1

Query: 59  LFHTRHNLPSLSLPFSSVPSLAFSCYNI-----GLKICALKAFRHAICLTFFL 106
           +FHT +N+     P S +P   F  + +      +K C  + F +  C+ FF+
Sbjct: 454 IFHTSNNIK----PHSLLPKNNFKVFILVKKF*NIKTCFFQDFSYGTCMIFFI 308


>TC14728 similar to UP|Q9LSL0 (Q9LSL0) Emb|CAB16809.1, partial (86%)
          Length = 1142

 Score = 26.6 bits (57), Expect = 6.9
 Identities = 15/39 (38%), Positives = 19/39 (48%)
 Frame = -2

Query: 67  PSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAICLTFF 105
           P  SL  SS  +   SC N+G  I  L  +   +CLT F
Sbjct: 658 PGASLVNSSYAASNASCQNLGNLILRLLVYTSHMCLTTF 542


>BU494245 
          Length = 482

 Score = 26.2 bits (56), Expect = 9.0
 Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 4/45 (8%)
 Frame = -2

Query: 48  FSGAFSSLFAHLFHTRHNLPS----LSLPFSSVPSLAFSCYNIGL 88
           F  AFS+   H+FH  H +      LS P     +LAF+   +GL
Sbjct: 268 FGCAFSNCHLHIFHGHHAILQRFLLLSAPVVEELALAFAGERLGL 134


>TC9029 similar to UP|Q7X9G7 (Q7X9G7) Alpha-L-arabinofuranosidase, partial
           (73%)
          Length = 1636

 Score = 26.2 bits (56), Expect = 9.0
 Identities = 13/29 (44%), Positives = 18/29 (61%)
 Frame = -1

Query: 117 HRKSVLSQIPSTNECPENIMINLNHEKSS 145
           HRK+ L+  PST + P N+M NL    +S
Sbjct: 865 HRKAFLNHSPSTKQPPGNLM-NLGFRSAS 782


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.320    0.135    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,423,325
Number of Sequences: 28460
Number of extensions: 92002
Number of successful extensions: 545
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 542
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 542
length of query: 357
length of database: 4,897,600
effective HSP length: 91
effective length of query: 266
effective length of database: 2,307,740
effective search space: 613858840
effective search space used: 613858840
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)


Medicago: description of AC144564.12