BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144541.4 - phase: 0
(412 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18914 278 9e-76
BI418768 213 6e-56
AW164008 42 2e-04
TC14527 similar to PIR|F86340|F86340 protein F2D10.34 [imported]... 40 7e-04
TC9277 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {Ara... 39 0.002
TC9362 similar to PIR|F86340|F86340 protein F2D10.34 [imported] ... 38 0.003
TC15641 38 0.003
TC9115 similar to PIR|T00425|T00425 photolyase/blue-light recept... 37 0.005
AV779072 35 0.023
BP076839 35 0.023
AV419203 35 0.030
TC15481 similar to UP|Q9LUR1 (Q9LUR1) RING zinc finger protein-l... 35 0.030
TC11796 similar to GB|AAP12870.1|30017273|BT006221 At4g35840 {Ar... 35 0.030
AV422284 34 0.051
TC14982 similar to UP|Q9FPI2 (Q9FPI2) At1g17620, partial (32%) 33 0.067
TC12569 UP|Q40221 (Q40221) Protein containing C-terminal RING-fi... 33 0.067
TC12446 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2... 33 0.067
TC16693 weakly similar to PIR|T51842|T51842 RING-H2 finger prote... 33 0.067
TC11970 similar to UP|Q9FQZ7 (Q9FQZ7) Avr9/Cf-9 rapidly elicited... 33 0.067
AV765793 33 0.087
>TC18914
Length = 524
Score = 278 bits (712), Expect = 9e-76
Identities = 134/172 (77%), Positives = 145/172 (83%), Gaps = 1/172 (0%)
Frame = +1
Query: 145 EHSAVSSGSHPCPYIAYVGPVHPSTSNSGGTVSEVSNFNHWNARPIHGDMSTSYTVPAVV 204
EH+AVSS SHPCPYIAY+GP+HPSTSNSGGTVSE SNF HWN P DM TSYT PAV
Sbjct: 7 EHTAVSSASHPCPYIAYIGPIHPSTSNSGGTVSEDSNFPHWNGPPRPSDMPTSYTYPAVD 186
Query: 205 LHYHSWDHHSSHFSSGSSHLGAADQPSVSQSNQRPTR-GSEATRSGSYMHPYPVGHSSVA 263
LHYHSW+HHSSHFSS +S LG ADQPSVS QRP R GSE RSGS++HP+ VGHSS A
Sbjct: 187 LHYHSWEHHSSHFSSATSRLGVADQPSVSAGTQRPARVGSEVPRSGSFVHPFLVGHSSGA 366
Query: 264 RAGNSAASSMIPPYPGSNARARDRVEALQAYYHQQQPPNSTTVRPPVASSAR 315
RAGNS ASSMIPPYPGSNARARDRV+ALQAYY QQPPNSTT+R PVASS+R
Sbjct: 367 RAGNSVASSMIPPYPGSNARARDRVQALQAYYQPQQPPNSTTMRTPVASSSR 522
>BI418768
Length = 546
Score = 213 bits (541), Expect = 6e-56
Identities = 98/126 (77%), Positives = 107/126 (84%), Gaps = 1/126 (0%)
Frame = +3
Query: 5 IDDGDGDTRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQC 64
+D GDG K SV CSICLE+V+DNGDRS+AKL CGHQFHLDCIGSAFN KGAMQC
Sbjct: 144 VDGGDGGGA--KGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQC 317
Query: 65 PNCRKIEKGQWLYANGSRSYPEFNMEDWTRDDDVYDLSYSEM-SLGVHWCPFGNFAQLPS 123
PNCRKIEKGQWLYANG RSYPEFNM+DWT D+D+YDLSYSE+ S GVHWCPFGN A+LPS
Sbjct: 318 PNCRKIEKGQWLYANGGRSYPEFNMDDWTYDEDLYDLSYSELQSFGVHWCPFGNLARLPS 497
Query: 124 SFEERE 129
FEE E
Sbjct: 498 PFEEGE 515
>AW164008
Length = 351
Score = 42.0 bits (97), Expect = 2e-04
Identities = 20/51 (39%), Positives = 32/51 (62%)
Frame = +3
Query: 18 SDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
SD+V C+IC +++ +S +L CGHQ+H DCI +++ + CP CR
Sbjct: 183 SDTVACAICKDLMGVG--QSAKRLPCGHQYHGDCIVPWLSSRNS--CPVCR 323
>TC14527 similar to PIR|F86340|F86340 protein F2D10.34 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(42%)
Length = 1095
Score = 40.0 bits (92), Expect = 7e-04
Identities = 21/60 (35%), Positives = 32/60 (53%)
Frame = +2
Query: 11 DTRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRKI 70
D+ R+ + C+ICL D GD QCGH FH+ CI + + + CP+CR++
Sbjct: 473 DSSPREWLATTECAICLAEF-DAGDEIRVLPQCGHGFHVGCIDTWLGSHSS--CPSCRQV 643
>TC9277 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {Arabidopsis
thaliana;}, partial (79%)
Length = 1297
Score = 38.9 bits (89), Expect = 0.002
Identities = 21/52 (40%), Positives = 26/52 (49%)
Frame = +3
Query: 17 SSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
S D + CS+CLE V N L C HQFH +CI +G CP C+
Sbjct: 864 SDDELTCSVCLEQV--NVGDILRSLPCLHQFHANCIDPWLRQQGT--CPVCK 1007
>TC9362 similar to PIR|F86340|F86340 protein F2D10.34 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(58%)
Length = 591
Score = 38.1 bits (87), Expect = 0.003
Identities = 20/48 (41%), Positives = 26/48 (53%)
Frame = +1
Query: 23 CSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRKI 70
C+ICL + GD QCGH FH+ CI + K CP+CR+I
Sbjct: 328 CAICLTEFAA-GDEIRVLPQCGHGFHVSCIDAWL--KSHSSCPSCRQI 462
>TC15641
Length = 525
Score = 38.1 bits (87), Expect = 0.003
Identities = 22/52 (42%), Positives = 26/52 (49%)
Frame = +2
Query: 17 SSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
SSD PC IC E + NG+ L CGH+FH CI K CP C+
Sbjct: 185 SSDPEPCCICQEQYA-NGE-IIGSLDCGHEFHTKCIKQWLVQKNL--CPICK 328
>TC9115 similar to PIR|T00425|T00425 photolyase/blue-light receptor (PHR2)
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (55%)
Length = 1018
Score = 37.4 bits (85), Expect = 0.005
Identities = 40/142 (28%), Positives = 56/142 (39%), Gaps = 5/142 (3%)
Frame = +1
Query: 206 HYHSWDHHSSHFSSGSSHLGAADQPSVSQSNQRPTRGSEATRSGSYMHPYPVGHSSV--- 262
H+HS HHS S SS + + N PTR ++S P P HS
Sbjct: 142 HHHSQ*HHSPFHSPPSSPSSNPNSHHLLPHNNNPTR----SKSQPKPPPSPTSHSPPPPH 309
Query: 263 ARAGNSAASSMIPPYPGSNARARDRVEALQAYYHQQQPPNSTTVRPPVASSARRSSGHSR 322
R N ++ PP P A P +T+ P S+A SSG +
Sbjct: 310 PRRPNPPSNPPSPPTP-------------SAPLTP*APTAPSTLPTPPHSAAPPSSGSAT 450
Query: 323 SAPLAS--LSSPPDQSGSYIYL 342
++ A+ LS+PP + S YL
Sbjct: 451 TSASATTRLSTPPTTNRSQFYL 516
>AV779072
Length = 531
Score = 35.0 bits (79), Expect = 0.023
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = -1
Query: 19 DSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCI 52
D+ PCS+C E D D L CGH +H CI
Sbjct: 390 DAEPCSVCQEEYDDGDD--IGSLYCGHFYHTGCI 295
>BP076839
Length = 392
Score = 35.0 bits (79), Expect = 0.023
Identities = 19/57 (33%), Positives = 25/57 (43%), Gaps = 2/57 (3%)
Frame = -3
Query: 14 HRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGS--AFNAKGAMQCPNCR 68
H S++ C ICL D + +L CGH FH C+ NA CP C+
Sbjct: 387 HMLSAEDAECCICLSAYDDGVE--LRQLPCGHHFHCACVDKWLCMNA----TCPLCK 235
>AV419203
Length = 393
Score = 34.7 bits (78), Expect = 0.030
Identities = 16/51 (31%), Positives = 24/51 (46%)
Frame = +1
Query: 18 SDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
+D C +CLE + + L C H FH++CI ++CP CR
Sbjct: 241 TDCSECPVCLEEFHEGNE--VRGLPCAHNFHVECIDEWLRLN--VKCPRCR 381
>TC15481 similar to UP|Q9LUR1 (Q9LUR1) RING zinc finger protein-like,
partial (32%)
Length = 706
Score = 34.7 bits (78), Expect = 0.030
Identities = 20/69 (28%), Positives = 35/69 (49%)
Frame = +1
Query: 23 CSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRKIEKGQWLYANGSR 82
C++CL ++G+ +C H FH DCI F++ CP CR + + + N ++
Sbjct: 424 CAVCLSEF-ESGETGRVLPKCKHAFHTDCIDMWFHSHST--CPLCRTLVEPE--PENQTQ 588
Query: 83 SYPEFNMED 91
+ PE + D
Sbjct: 589 TRPELVLVD 615
>TC11796 similar to GB|AAP12870.1|30017273|BT006221 At4g35840 {Arabidopsis
thaliana;}, partial (86%)
Length = 1114
Score = 34.7 bits (78), Expect = 0.030
Identities = 20/59 (33%), Positives = 27/59 (44%)
Frame = +1
Query: 11 DTRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRK 69
D S + V CS+CL+ G+ + C H FHL CI G+ CP CR+
Sbjct: 574 DNNADASGERVSCSVCLQDFQ-LGETVRSLPHCHHMFHLPCIDKWLFRHGS--CPLCRR 741
>AV422284
Length = 471
Score = 33.9 bits (76), Expect = 0.051
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +2
Query: 19 DSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCI 52
D V C++CL + D G+ +C H FH+DCI
Sbjct: 368 DPVECAVCLSELED-GETGRVLPKCNHTFHVDCI 466
>TC14982 similar to UP|Q9FPI2 (Q9FPI2) At1g17620, partial (32%)
Length = 1036
Score = 33.5 bits (75), Expect = 0.067
Identities = 33/117 (28%), Positives = 43/117 (36%), Gaps = 3/117 (2%)
Frame = +3
Query: 219 SGSSHLGAADQPSVS---QSNQRPTRGSEATRSGSYMHPYPVGHSSVARAGNSAASSMIP 275
S ++ AA PS S S+ + S S S P S + NS S+ P
Sbjct: 162 STAAPAAAASAPSASG*SSSSSSSSSSSAVLASPSTSSTAPTTPPSPSPPSNSPTSTSPP 341
Query: 276 PYPGSNARARDRVEALQAYYHQQQPPNSTTVRPPVASSARRSSGHSRSAPLASLSSP 332
P P PPNST+ PP + R + HS + PL S SP
Sbjct: 342 PQPS--------------------PPNSTSPSPP--QTPTRKTSHSPTYPLPSPYSP 446
>TC12569 UP|Q40221 (Q40221) Protein containing C-terminal RING-finger,
complete
Length = 2092
Score = 33.5 bits (75), Expect = 0.067
Identities = 18/53 (33%), Positives = 25/53 (46%)
Frame = +2
Query: 16 KSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
+ D C ICLE + D K CGH +H++CI + K CP C+
Sbjct: 1763 QGQDEGSCVICLEEYKNMDDVGTLKT-CGHDYHVNCIKKWLSMK--KLCPICK 1912
>TC12446 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (38%)
Length = 550
Score = 33.5 bits (75), Expect = 0.067
Identities = 18/45 (40%), Positives = 24/45 (53%)
Frame = +2
Query: 23 CSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNC 67
CSICLE + D+ + C H+FHL CI + + QCP C
Sbjct: 203 CSICLEDLCDSEPSTVTG--CKHEFHLQCILEW--CQRSSQCPMC 325
>TC16693 weakly similar to PIR|T51842|T51842 RING-H2 finger protein RHA2a
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (35%)
Length = 442
Score = 33.5 bits (75), Expect = 0.067
Identities = 17/51 (33%), Positives = 22/51 (42%)
Frame = +2
Query: 19 DSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRK 69
D C +CL + +L+C H FH DC+ A CP CRK
Sbjct: 221 DDTECRVCLSEFEEG--EKVRRLKCKHTFHKDCLDKWLQECWA-TCPLCRK 364
>TC11970 similar to UP|Q9FQZ7 (Q9FQZ7) Avr9/Cf-9 rapidly elicited protein
132, partial (24%)
Length = 789
Score = 33.5 bits (75), Expect = 0.067
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = +1
Query: 25 ICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
+CL + D G+++ +C H FH+DCI F + CP CR
Sbjct: 4 VCLSEL-DEGEKARLLPKCNHGFHVDCIDMWFQSHST--CPLCR 126
>AV765793
Length = 592
Score = 33.1 bits (74), Expect = 0.087
Identities = 17/53 (32%), Positives = 25/53 (47%)
Frame = -3
Query: 34 GDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRKIEKGQWLYANGSRSYPE 86
G+++ +C H FH+DCI F + CP CR + NGS + E
Sbjct: 578 GEKARLLPKCNHGFHVDCIDMWFQSHST--CPLCRNPVASESCKPNGSSNAEE 426
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.128 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,954,680
Number of Sequences: 28460
Number of extensions: 154846
Number of successful extensions: 1139
Number of sequences better than 10.0: 158
Number of HSP's better than 10.0 without gapping: 1071
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1126
length of query: 412
length of database: 4,897,600
effective HSP length: 92
effective length of query: 320
effective length of database: 2,279,280
effective search space: 729369600
effective search space used: 729369600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 56 (26.2 bits)
Medicago: description of AC144541.4