BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144541.1 - phase: 0
(124 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19834 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein ... 222 1e-59
TC20261 similar to UP|R22C_ARATH (Q9FE58) 60S ribosomal protein ... 179 9e-47
BP085563 30 0.12
TC9663 similar to UP|Q9FFK7 (Q9FFK7) Signal recognition particle... 27 0.78
TC10870 similar to GB|CAA77438.1|2924285|CHNTXX Ycf2 protein {Ni... 25 3.0
TC8750 weakly similar to UP|Q39821 (Q39821) SDL12A, partial (10%) 25 3.9
BP082957 25 5.1
TC8032 similar to UP|Q9M0B6 (Q9M0B6) Nucleotide sugar epimerase-... 25 5.1
AV772649 24 6.6
TC9306 similar to UP|Q42922 (Q42922) Annexin (Fragment), partial... 24 8.7
>TC19834 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein L22-2,
partial (94%)
Length = 430
Score = 222 bits (566), Expect = 1e-59
Identities = 109/118 (92%), Positives = 115/118 (97%)
Frame = +2
Query: 7 AGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTREKSKI 66
+GAKGKKK +F IDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSV+V+REKSKI
Sbjct: 32 SGAKGKKKATSFVIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVTVSREKSKI 211
Query: 67 TVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEGEEED 124
TVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDR++YELRYFNIAENEGEEED
Sbjct: 212 TVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRNVYELRYFNIAENEGEEED 385
>TC20261 similar to UP|R22C_ARATH (Q9FE58) 60S ribosomal protein L22-3,
partial (80%)
Length = 352
Score = 179 bits (455), Expect = 9e-47
Identities = 89/99 (89%), Positives = 93/99 (93%)
Frame = +2
Query: 1 MSRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVT 60
MSRG AAGAKGKKK TFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGD VS+
Sbjct: 56 MSRGVAAGAKGKKKAVTFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDVVSIA 235
Query: 61 REKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVI 99
R+K+KI+VTSDSNFSKRYLKYLTKKYLKKHNVRDWLR I
Sbjct: 236 RDKNKISVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRXI 352
>BP085563
Length = 338
Score = 30.0 bits (66), Expect = 0.12
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +2
Query: 59 VTREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVI 99
++ KSK DS FS+ ++L KY+ K N R W + +
Sbjct: 5 ISNLKSKFMRWQDSIFSRNLKRHLYNKYISKLNPRFWAQTL 127
>TC9663 similar to UP|Q9FFK7 (Q9FFK7) Signal recognition particle receptor
beta subunit-like protein, partial (44%)
Length = 612
Score = 27.3 bits (59), Expect = 0.78
Identities = 12/35 (34%), Positives = 20/35 (56%)
Frame = -1
Query: 80 KYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFN 114
K+ TKK KHN+ WL + + +++LRY +
Sbjct: 387 KFNTKKSKFKHNIHLWLHIFSD----ELFQLRYLS 295
>TC10870 similar to GB|CAA77438.1|2924285|CHNTXX Ycf2 protein {Nicotiana
tabacum;}, complete
Length = 6897
Score = 25.4 bits (54), Expect = 3.0
Identities = 12/31 (38%), Positives = 16/31 (50%)
Frame = +1
Query: 11 GKKKGATFTIDCAKPVEDKIMDIASLEKFLQ 41
GKKK A D P+E ++ +I L F Q
Sbjct: 3232 GKKKHAFLERDTISPIESRVFNILILNDFPQ 3324
>TC8750 weakly similar to UP|Q39821 (Q39821) SDL12A, partial (10%)
Length = 588
Score = 25.0 bits (53), Expect = 3.9
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +2
Query: 72 SNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELR 111
S +L +L KYL+ + + + I S DRSI ELR
Sbjct: 41 SMMGSEHLAWLLSKYLEDWIIVNRIPAIQSFIDRSIRELR 160
>BP082957
Length = 230
Score = 24.6 bits (52), Expect = 5.1
Identities = 9/39 (23%), Positives = 21/39 (53%)
Frame = -3
Query: 73 NFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELR 111
+F+ R L + K+ + ++ WL + N+ +Y+L+
Sbjct: 225 SFNSRVLNFTIKRVIYRNGDDSWLGCMYQNRRVHVYDLK 109
>TC8032 similar to UP|Q9M0B6 (Q9M0B6) Nucleotide sugar epimerase-like
protein, partial (97%)
Length = 2230
Score = 24.6 bits (52), Expect = 5.1
Identities = 27/98 (27%), Positives = 39/98 (39%), Gaps = 20/98 (20%)
Frame = +3
Query: 4 GSAAGAKGKKKGAT----FTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGAL-----G 54
G + G+ GKKKGA F + PV + +L L+ +KV K + G
Sbjct: 1140 GKSTGSGGKKKGAAPYRIFNLGNTSPV-----TVPTLVSILEGHLKVKAKRNVVDMPGNG 1304
Query: 55 D------SVSVTREKSKITVTSD-----SNFSKRYLKY 81
D ++S R + T+D F K YL Y
Sbjct: 1305 DVPFTHANISSARRELGYKPTTDLQTGLKKFVKWYLSY 1418
>AV772649
Length = 461
Score = 24.3 bits (51), Expect = 6.6
Identities = 16/43 (37%), Positives = 20/43 (46%), Gaps = 1/43 (2%)
Frame = +2
Query: 57 VSVTREKSKITVTSDSNFSKRYLKYL-TKKYLKKHNVRDWLRV 98
+ V + T T +K K L T KY KKHN+ LRV
Sbjct: 113 IGVASRANMSTATGRRTQAKGLRKILPTNKYNKKHNILK*LRV 241
>TC9306 similar to UP|Q42922 (Q42922) Annexin (Fragment), partial (59%)
Length = 662
Score = 23.9 bits (50), Expect = 8.7
Identities = 11/31 (35%), Positives = 19/31 (60%)
Frame = -3
Query: 43 RIKVGGKAGALGDSVSVTREKSKITVTSDSN 73
R + G+ + SV ++K K+T+T+DSN
Sbjct: 117 RRNLQGRHCVIKISVKKKKKKKKVTLTNDSN 25
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.312 0.131 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,422,572
Number of Sequences: 28460
Number of extensions: 12351
Number of successful extensions: 55
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 55
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 55
length of query: 124
length of database: 4,897,600
effective HSP length: 79
effective length of query: 45
effective length of database: 2,649,260
effective search space: 119216700
effective search space used: 119216700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 50 (23.9 bits)
Medicago: description of AC144541.1