BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144516.7 - phase: 0
(261 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15211 similar to UP|Q9ZRD9 (Q9ZRD9) GMFP4 (Fragment), partial ... 180 2e-46
AW720075 144 1e-35
AV408321 138 8e-34
TC15210 similar to UP|Q9ZRD9 (Q9ZRD9) GMFP4 (Fragment), partial ... 112 5e-26
TC13605 homologue to UP|Q9ZRD9 (Q9ZRD9) GMFP4 (Fragment), partia... 50 9e-11
TC16748 similar to GB|AAO64917.1|29029076|BT005982 At4g28770 {Ar... 48 1e-06
TC13554 46 6e-06
AV766067 37 0.003
TC20146 weakly similar to UP|Q940P5 (Q940P5) At2g20740/F5H14.29,... 35 0.017
AV411760 33 0.065
TC14083 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor... 31 0.25
AU251384 27 2.7
AV426158 27 2.7
TC9990 similar to UP|Q9FJW0 (Q9FJW0) RuvB DNA helicase-like prot... 27 3.5
AV423931 27 4.6
TC10598 weakly similar to GB|AAM78036.1|21928015|AY125526 At2g01... 27 4.6
BP037361 26 6.1
TC9403 similar to UP|Q9LXC9 (Q9LXC9) Inorganic pyrophosphatase-l... 26 7.9
TC11198 similar to UP|Q9LJT8 (Q9LJT8) Non-race specific disease ... 26 7.9
TC8131 similar to UP|Q9XFM6 (Q9XFM6) Progesterone-binding protei... 26 7.9
>TC15211 similar to UP|Q9ZRD9 (Q9ZRD9) GMFP4 (Fragment), partial (94%)
Length = 531
Score = 180 bits (457), Expect = 2e-46
Identities = 88/109 (80%), Positives = 95/109 (86%)
Frame = +2
Query: 153 IFEALLFLLALIVRAVNRPADYDSDDELINPRQQVRQPLLNNRQAGPPSGLPVAGTTDQR 212
+FE LLFLLALIVRA NRPADYDSD+E INPRQQ RQPLLN R AGP +G P +GT DQR
Sbjct: 2 VFEVLLFLLALIVRASNRPADYDSDEEFINPRQQARQPLLN-RPAGPATGPPASGTLDQR 178
Query: 213 PNRNDAWSTRMREKYGLDTSEFTYNPSESQRFQQANSQPTEEKSRCTIM 261
P+RNDAWSTRMREKYGLDTSEFTYNPSES RFQ N+ PTEE+SRCTIM
Sbjct: 179 PSRNDAWSTRMREKYGLDTSEFTYNPSESPRFQPVNAPPTEERSRCTIM 325
>AW720075
Length = 436
Score = 144 bits (364), Expect = 1e-35
Identities = 70/100 (70%), Positives = 79/100 (79%)
Frame = +1
Query: 2 VCCRGFWECLLKLLNFILTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSGDS 61
+ CRGFWECLLKL NFI+T+ GL +VGYGIYLLV F KAS D TP +SP S D
Sbjct: 148 MACRGFWECLLKLFNFIMTLIGLTMVGYGIYLLVEFIKASGSD----DDTPAVSPASDDQ 315
Query: 62 ALIQFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLF 101
ALIQ GRPML+A SLS++IFDKLPKAWFIYLFIG+G VLF
Sbjct: 316 ALIQLGRPMLLAXSLSESIFDKLPKAWFIYLFIGIGVVLF 435
>AV408321
Length = 348
Score = 138 bits (348), Expect = 8e-34
Identities = 70/102 (68%), Positives = 80/102 (77%), Gaps = 1/102 (0%)
Frame = +1
Query: 1 MVC-CRGFWECLLKLLNFILTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSG 59
M C CRG WECLLKLLNF+LT+ GLA+VGYGIYLLV F+KASD+ T +S VS
Sbjct: 64 MACACRGCWECLLKLLNFLLTLVGLAMVGYGIYLLVEFSKASDN-------TLSVSTVSD 222
Query: 60 DSALIQFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLF 101
D L+Q GRPMLMAVSLS++ FD LPKAWFIYLFIG+G VLF
Sbjct: 223 DQTLVQLGRPMLMAVSLSNSFFDDLPKAWFIYLFIGIGVVLF 348
>TC15210 similar to UP|Q9ZRD9 (Q9ZRD9) GMFP4 (Fragment), partial (54%)
Length = 523
Score = 112 bits (281), Expect = 5e-26
Identities = 51/61 (83%), Positives = 56/61 (91%)
Frame = +1
Query: 201 SGLPVAGTTDQRPNRNDAWSTRMREKYGLDTSEFTYNPSESQRFQQANSQPTEEKSRCTI 260
+G P +GT DQRP+RNDAWSTRMREKYGLDTSEFTYNPSESQRFQQ N+ PTEE+SRCTI
Sbjct: 4 TGPPASGTLDQRPSRNDAWSTRMREKYGLDTSEFTYNPSESQRFQQVNAPPTEERSRCTI 183
Query: 261 M 261
M
Sbjct: 184 M 186
>TC13605 homologue to UP|Q9ZRD9 (Q9ZRD9) GMFP4 (Fragment), partial (33%)
Length = 399
Score = 49.7 bits (117), Expect(2) = 9e-11
Identities = 21/21 (100%), Positives = 21/21 (100%)
Frame = +3
Query: 218 AWSTRMREKYGLDTSEFTYNP 238
AWSTRMREKYGLDTSEFTYNP
Sbjct: 3 AWSTRMREKYGLDTSEFTYNP 65
Score = 32.3 bits (72), Expect(2) = 9e-11
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = +2
Query: 242 QRFQQANSQPTEEKSRCTIM 261
Q Q NSQPTEE+SRC IM
Sbjct: 77 QGSSQVNSQPTEERSRCAIM 136
>TC16748 similar to GB|AAO64917.1|29029076|BT005982 At4g28770 {Arabidopsis
thaliana;}, partial (36%)
Length = 442
Score = 48.1 bits (113), Expect = 1e-06
Identities = 31/108 (28%), Positives = 52/108 (47%)
Frame = +1
Query: 3 CCRGFWECLLKLLNFILTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSGDSA 62
CC +LK LNF+ G++I+ Y +++L + D + P P IS
Sbjct: 79 CCHVSLAFVLKFLNFLQAFVGVSILLYSVWMLNQW-----DHRVPQPPLPHIS------- 222
Query: 63 LIQFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCIA 110
P +++ +++ LP WFIY F+GVG ++ ++ GCIA
Sbjct: 223 -----LPRIVSAINVNSL--NLPAPWFIYAFMGVGILMCCITFLGCIA 345
>TC13554
Length = 441
Score = 46.2 bits (108), Expect = 6e-06
Identities = 30/70 (42%), Positives = 40/70 (56%), Gaps = 1/70 (1%)
Frame = +3
Query: 170 RPADYDSDDELINPRQQVRQPLLNNRQAGPPSGLPV-AGTTDQRPNRNDAWSTRMREKYG 228
R AD+D +DE + R + +PLLN PPSG P +G D R N +D WS+RMREK
Sbjct: 27 RRADFDYEDEY-DVRVRSWEPLLN-----PPSGQPSGSGQVDNRGNPSDIWSSRMREKVR 188
Query: 229 LDTSEFTYNP 238
+ T + P
Sbjct: 189 I*TMVISQAP 218
>AV766067
Length = 350
Score = 37.0 bits (84), Expect = 0.003
Identities = 20/40 (50%), Positives = 27/40 (67%)
Frame = -3
Query: 190 PLLNNRQAGPPSGLPVAGTTDQRPNRNDAWSTRMREKYGL 229
PLLN Q+G PSG +G + R N + WS+RM++KYGL
Sbjct: 348 PLLNP-QSGHPSG---SGKVNNRGNPSPIWSSRMKKKYGL 241
>TC20146 weakly similar to UP|Q940P5 (Q940P5) At2g20740/F5H14.29, partial
(45%)
Length = 417
Score = 34.7 bits (78), Expect = 0.017
Identities = 25/106 (23%), Positives = 44/106 (40%)
Frame = +1
Query: 5 RGFWECLLKLLNFILTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSGDSALI 64
R + +LKL+N ++ + GLA++ Y +++ + + +
Sbjct: 115 RSCMQSILKLVNSVMGMAGLAMILYSAWMMRVRQRQMGE--------------------L 234
Query: 65 QFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCIA 110
FG SDN P WFIY +G+G V++C G +A
Sbjct: 235 PFGHD-------SDN-----PPPWFIYTLLGLGVTFCVITCPGHVA 336
>AV411760
Length = 420
Score = 32.7 bits (73), Expect = 0.065
Identities = 14/26 (53%), Positives = 16/26 (60%)
Frame = -2
Query: 89 FIYLFIGVGAVLFVVSCFGCIAFLFF 114
F LF+GV A F VSCF +F FF
Sbjct: 335 FFSLFLGVSATFFFVSCFSSSSFTFF 258
>TC14083 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor, partial
(92%)
Length = 1693
Score = 30.8 bits (68), Expect = 0.25
Identities = 16/53 (30%), Positives = 25/53 (46%)
Frame = -2
Query: 71 LMAVSLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCIAFLFFDKSWKEEIP 123
L+ +S+ D +D + WF L + VLFV+ F + D W E+ P
Sbjct: 867 LLGISIWDRFWDVIIPLWF--LIFRIRNVLFVIPVFWLLGLRILDFLWWEKTP 715
>AU251384
Length = 364
Score = 27.3 bits (59), Expect = 2.7
Identities = 9/19 (47%), Positives = 12/19 (62%)
Frame = -1
Query: 102 VVSCFGCIAFLFFDKSWKE 120
V+ CFGC + +SWKE
Sbjct: 124 VIGCFGCQTYSHSSRSWKE 68
>AV426158
Length = 274
Score = 27.3 bits (59), Expect = 2.7
Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Frame = +1
Query: 1 MVCCRGFWECLLKLLNFILTVT--GLAIVGYGIYLLV 35
M+CC F CL+ L++ I+ VT G I G G L+
Sbjct: 163 MICCTRFLTCLVILISSIILVTLDGKIISGRGRLSLI 273
>TC9990 similar to UP|Q9FJW0 (Q9FJW0) RuvB DNA helicase-like protein
(AT5g67630/K9I9_20), partial (39%)
Length = 642
Score = 26.9 bits (58), Expect = 3.5
Identities = 15/54 (27%), Positives = 23/54 (41%)
Frame = +1
Query: 194 NRQAGPPSGLPVAGTTDQRPNRNDAWSTRMREKYGLDTSEFTYNPSESQRFQQA 247
+R+ PP +RP R AW+ + R ++G NP R Q+A
Sbjct: 229 SRRRHPPDD*GRQNRRSRRPPRRPAWNRQDRHRHGYGQVPRPRNPLRHDRRQRA 390
>AV423931
Length = 390
Score = 26.6 bits (57), Expect = 4.6
Identities = 19/61 (31%), Positives = 25/61 (40%), Gaps = 1/61 (1%)
Frame = -3
Query: 195 RQAGPPSGLPVA-GTTDQRPNRNDAWSTRMREKYGLDTSEFTYNPSESQRFQQANSQPTE 253
RQ P L A G D RP++ND T R + E + N Q S+P E
Sbjct: 349 RQKEPGEALSGAFGGADPRPSKNDERDTTSRARSVSGEEETSGNDKNMDLHCQGRSRPPE 170
Query: 254 E 254
+
Sbjct: 169 K 167
>TC10598 weakly similar to GB|AAM78036.1|21928015|AY125526
At2g01100/F23H14.7 {Arabidopsis thaliana;}, partial
(33%)
Length = 902
Score = 26.6 bits (57), Expect = 4.6
Identities = 20/84 (23%), Positives = 40/84 (46%)
Frame = +3
Query: 172 ADYDSDDELINPRQQVRQPLLNNRQAGPPSGLPVAGTTDQRPNRNDAWSTRMREKYGLDT 231
+DYDSDDE + R R + +++ S R + S R +K+ ++
Sbjct: 417 SDYDSDDE--SKRTSRRSHRKHKKRSHYDS---------DHEKRKEKLSKRKTKKWSSES 563
Query: 232 SEFTYNPSESQRFQQANSQPTEEK 255
S+F+ + SES+ ++ + ++K
Sbjct: 564 SDFSGDESESRSEEERRRKRKQKK 635
>BP037361
Length = 524
Score = 26.2 bits (56), Expect = 6.1
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = +1
Query: 3 CCRGFWECLLKLLNFILTVTGLAIVGYGIYLLVAF 37
CC W CLL+ I + LA+ G + L + F
Sbjct: 220 CCIRMWHCLLRRRRRIFLLLRLALEGCWLRLRLCF 324
>TC9403 similar to UP|Q9LXC9 (Q9LXC9) Inorganic pyrophosphatase-like
protein , partial (45%)
Length = 819
Score = 25.8 bits (55), Expect = 7.9
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -3
Query: 3 CCRGFWECLLKLLNFI 18
CC G W+ LL ++N I
Sbjct: 232 CCEGSWKMLLNIINII 185
>TC11198 similar to UP|Q9LJT8 (Q9LJT8) Non-race specific disease resistance
protein-like, partial (17%)
Length = 882
Score = 25.8 bits (55), Expect = 7.9
Identities = 13/33 (39%), Positives = 14/33 (42%)
Frame = +3
Query: 37 FAKASDDDKPDISPTPDISPVSGDSALIQFGRP 69
FA AS P P +P SGDS RP
Sbjct: 297 FASASPSSSTTTPPAPSATPPSGDSTRATRRRP 395
>TC8131 similar to UP|Q9XFM6 (Q9XFM6) Progesterone-binding protein-like,
partial (67%)
Length = 1227
Score = 25.8 bits (55), Expect = 7.9
Identities = 18/56 (32%), Positives = 26/56 (46%)
Frame = -2
Query: 19 LTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSGDSALIQFGRPMLMAV 74
+TVT + IV YLL+ S P P+ISPV S+ F + L ++
Sbjct: 566 VTVTVVLIVPTFTYLLMNLYSQSCKASSSNEPRPEISPVRSFSSKDIFAKARLASL 399
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.141 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,844,699
Number of Sequences: 28460
Number of extensions: 67404
Number of successful extensions: 541
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 536
length of query: 261
length of database: 4,897,600
effective HSP length: 88
effective length of query: 173
effective length of database: 2,393,120
effective search space: 414009760
effective search space used: 414009760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144516.7