BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144516.4 + phase: 0
(360 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP038942 129 1e-40
TC16587 weakly similar to UP|Q9JGS5 (Q9JGS5) PORF1, partial (3%) 81 2e-16
BP045743 80 4e-16
TC18339 weakly similar to GB|AAF21246.1|6648604|AF048705 ubiquit... 33 0.097
BP036655 33 0.097
TC11985 similar to GB|AAF40452.1|7211981|F13M7 ESTs gb|N65605, g... 32 0.17
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 32 0.17
AV764893 31 0.28
TC11270 similar to UP|Q9LM88 (Q9LM88) F2D10.15, partial (9%) 31 0.28
TC8557 similar to UP|Q9LK52 (Q9LK52) Dbj|BAA90629.1, complete 31 0.28
TC12573 similar to UP|Q9CB03 (Q9CB03) F17L21.25 (AT1G27460/F17L2... 31 0.37
AV409070 30 0.63
BP043430 30 0.63
BP064526 30 0.63
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 29 1.1
TC19735 similar to UP|Q8NJM5 (Q8NJM5) Probable ATP-dependent RNA... 29 1.1
BP055303 29 1.1
AV409550 29 1.4
AV416769 29 1.4
TC13932 similar to UP|Q38901 (Q38901) Cytosolic cyclophilin (Pe... 29 1.4
>BP038942
Length = 617
Score = 129 bits (324), Expect(2) = 1e-40
Identities = 77/120 (64%), Positives = 90/120 (74%), Gaps = 13/120 (10%)
Frame = +3
Query: 51 SFSLSTNFPTGPTFKLSYTPTTTSSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSLS--- 107
SFSLSTNFPTGP+FKL+Y+PT SS PFSLSLKSGLG GSP HSPL FSAN SLS
Sbjct: 258 SFSLSTNFPTGPSFKLAYSPTA-SSLPPFSLSLKSGLGTSGSPRHSPLTFSANLSLSLSP 434
Query: 108 SLSSSTPLLPSFSLHFKPQFGHFSLHKTVLSDSSNTNPLSV--SPQIE--------KGFV 157
+ ++ TP +PSF LHFKPQFGHFSL+KTV SD NT+P+S+ +PQ + KGFV
Sbjct: 435 TTNTFTP-VPSFFLHFKPQFGHFSLNKTVFSD-PNTDPVSLPTAPQPDHRESQXGRKGFV 608
Score = 53.9 bits (128), Expect(2) = 1e-40
Identities = 28/52 (53%), Positives = 34/52 (64%), Gaps = 1/52 (1%)
Frame = +2
Query: 1 MKLSLKFQNNN-ENQQTPQTQIMTAKVPITIFNNPLLSTITATNNYSTSDFS 51
MKLSLKF + E Q TQIMTAKVPIT+ NNP +S + AT + S+ S
Sbjct: 95 MKLSLKFHGDGTEEDQQHSTQIMTAKVPITVLNNPFISAVKATTTTANSESS 250
>TC16587 weakly similar to UP|Q9JGS5 (Q9JGS5) PORF1, partial (3%)
Length = 706
Score = 81.3 bits (199), Expect = 2e-16
Identities = 50/135 (37%), Positives = 77/135 (57%)
Frame = +3
Query: 1 MKLSLKFQNNNENQQTPQTQIMTAKVPITIFNNPLLSTITATNNYSTSDFSFSLSTNFPT 60
MK SLKF+ + Q+ P + AKVP+++ P S I A ++ + + +L+T F +
Sbjct: 195 MKASLKFR---DEQKKP---LFRAKVPLSVLGMPFQSGIVAGDS---KELTLNLATFFQS 347
Query: 61 GPTFKLSYTPTTTSSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSLSSLSSSTPLLPSFS 120
GP+ + SY P + + PFS+ +K+G G FGSP ++ S F+L + S P F
Sbjct: 348 GPSIRASYRPNDSKN--PFSVVVKTGTGPFGSPLSGSMLMSCEFNLLARSGG----PLFM 509
Query: 121 LHFKPQFGHFSLHKT 135
LHFKP+FG F+ KT
Sbjct: 510 LHFKPRFGDFTFKKT 554
>BP045743
Length = 512
Score = 80.5 bits (197), Expect = 4e-16
Identities = 50/96 (52%), Positives = 61/96 (63%), Gaps = 13/96 (13%)
Frame = -1
Query: 276 MKIDLENVEKENKEMKRVLDEMKMGVS--------KGKVSKQ-----KHAGESVQSWGSN 322
MK DLENVEKENKEMKRVL+EMK+GVS G V K+ Q W S+
Sbjct: 509 MKRDLENVEKENKEMKRVLEEMKVGVSTSYREASINGNVEKRLSPPLGEVSSEFQRWRSS 330
Query: 323 KNGRKENEKKQEIKPQNIVVSDFESELEKAIKAAAA 358
++ R ENEKKQ K + V SD E+EL+KAIKAA++
Sbjct: 329 QSDRPENEKKQPDKSPS-VASDLEAELQKAIKAASS 225
>TC18339 weakly similar to GB|AAF21246.1|6648604|AF048705 ubiquitin-specific
protease {Arabidopsis thaliana;}, partial (12%)
Length = 500
Score = 32.7 bits (73), Expect = 0.097
Identities = 20/39 (51%), Positives = 21/39 (53%)
Frame = -3
Query: 68 YTPTTTSSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSL 106
YT T SSSLP SLSL L L SP L S +F L
Sbjct: 156 YTSTPLSSSLPLSLSLSLSLSLSLSPPQPSLQVSLSFLL 40
>BP036655
Length = 556
Score = 32.7 bits (73), Expect = 0.097
Identities = 22/65 (33%), Positives = 34/65 (51%), Gaps = 1/65 (1%)
Frame = -3
Query: 250 ERVEEMKKDEWNNDDNCEGNLQVLKEMKIDLENVEKENKEMKRVLDEMKMGVSK-GKVSK 308
E VEE D+ NDD + ++ E +I+ E E+E +E E +MGV K + S+
Sbjct: 353 EVVEEWSDDDDINDDESDDEVEEESETEIETEKEEEEEEECGVENVEDEMGVKKFNRPSR 174
Query: 309 QKHAG 313
K +G
Sbjct: 173 CKESG 159
Score = 26.9 bits (58), Expect = 5.3
Identities = 26/83 (31%), Positives = 39/83 (46%)
Frame = +1
Query: 31 FNNPLLSTITATNNYSTSDFSFSLSTNFPTGPTFKLSYTPTTTSSSLPFSLSLKSGLGLF 90
F P+ S+ +T + S+S SFS+S + ++TSSSL SL S L
Sbjct: 193 FFTPISSSTFSTPHSSSSSSSFSVSISVSLS---------SSTSSSLSSSLISSSSL--- 336
Query: 91 GSPNHSPLVFSANFSLSSLSSST 113
HS S++ + SS S S+
Sbjct: 337 ----HSSTTSSSSSTTSSSSPSS 393
>TC11985 similar to GB|AAF40452.1|7211981|F13M7 ESTs gb|N65605, gb|N38087,
gb|T20485, gb|T13726, gb|N38339, gb|F15440 and gb|N97201
come from this gene. {Arabidopsis thaliana;}, partial
(88%)
Length = 1334
Score = 32.0 bits (71), Expect = 0.17
Identities = 32/104 (30%), Positives = 49/104 (46%), Gaps = 3/104 (2%)
Frame = -3
Query: 41 ATNNYSTSDFSFSLSTNFPTG--PTFKLSYTPTTTSSSLPFSLSL-KSGLGLFGSPNHSP 97
A N++S S L+ + +G P+ +L ++++ L FSL+L S F NH
Sbjct: 792 ADNSFSPLPQSTGLAADGSSGGNPSLRLLSALSSSNFCLIFSLALLSSSFSAFL*ANHRF 613
Query: 98 LVFSANFSLSSLSSSTPLLPSFSLHFKPQFGHFSLHKTVLSDSS 141
L S++F +S +S + SFSL F S LS SS
Sbjct: 612 LSLSSSFLFASSNSLPIRILSFSLSLSILFSSSSFFLRALSLSS 481
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 32.0 bits (71), Expect = 0.17
Identities = 22/55 (40%), Positives = 30/55 (54%)
Frame = -3
Query: 59 PTGPTFKLSYTPTTTSSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSLSSLSSST 113
P P+F P+T++SSLP S S S SP+ S S++ S SS SSS+
Sbjct: 418 PLAPSF-----PSTSTSSLPSSSSSSSSSSSSSSPSSSSSSSSSSSSSSSSSSSS 269
>AV764893
Length = 539
Score = 31.2 bits (69), Expect = 0.28
Identities = 23/83 (27%), Positives = 39/83 (46%)
Frame = -2
Query: 249 LERVEEMKKDEWNNDDNCEGNLQVLKEMKIDLENVEKENKEMKRVLDEMKMGVSKGKVSK 308
+E E+++KDE N N N + + ++ + K + ++K D MK V
Sbjct: 520 VEVTEKVEKDEENVQGNVSSNDLKTQVISVN-KRKRKRSAQVKEKTDPMK---EDNGVRA 353
Query: 309 QKHAGESVQSWGSNKNGRKENEK 331
+ A ESV+S G +NG + K
Sbjct: 352 KLSADESVKSTGFRQNGSRRKSK 284
>TC11270 similar to UP|Q9LM88 (Q9LM88) F2D10.15, partial (9%)
Length = 735
Score = 31.2 bits (69), Expect = 0.28
Identities = 35/100 (35%), Positives = 47/100 (47%), Gaps = 7/100 (7%)
Frame = -3
Query: 33 NPLLSTITATNNYST--SDFSFSLSTNFPTGPTFKLSYTPTTTSSSLPFSLSLKSGLGLF 90
+PL ST + +T S S S S++FP + + T +++SSS PFS S S
Sbjct: 580 SPLESTFLPFTSTTTTPSSSSSSSSSSFPCSSS---TTTSSSSSSSSPFSSSSSSSCACS 410
Query: 91 --GSPNHSPLVFSANFSLSSLSSSTP---LLPSFSLHFKP 125
P V S++ S SS SSSTP S S F P
Sbjct: 409 TRDLPRVGCGVCSSSSSSSSSSSSTPWFSFSTSLSFFFLP 290
>TC8557 similar to UP|Q9LK52 (Q9LK52) Dbj|BAA90629.1, complete
Length = 1064
Score = 31.2 bits (69), Expect = 0.28
Identities = 35/98 (35%), Positives = 48/98 (48%), Gaps = 1/98 (1%)
Frame = -2
Query: 53 SLSTNFPTGPTFKLSYTPTTTSSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSLSSLSSS 112
SL F + + + TT+SS L F+L +GL G P S SA+F+LSS S S
Sbjct: 787 SLMNRFSALASPRAWFLNTTSSSHLLFTLK-DAGLFSNGFPRRS----SASFTLSSSSCS 623
Query: 113 TPLLPSFSLHFKPQFGHFSLHK-TVLSDSSNTNPLSVS 149
+ SFS S K +V S SS+++ LS S
Sbjct: 622 SRSFRSFSSALSFFICSSSASKASVSSSSSSSSSLSSS 509
>TC12573 similar to UP|Q9CB03 (Q9CB03) F17L21.25 (AT1G27460/F17L21_26),
partial (5%)
Length = 609
Score = 30.8 bits (68), Expect = 0.37
Identities = 16/52 (30%), Positives = 19/52 (35%)
Frame = +1
Query: 123 FKPQFGHFSLHKTVLSDSSNTNPLSVSPQIEKGFVPETNGSCSGWQNLNLEP 174
F+ F+ H L S SP EK V +G C NLN P
Sbjct: 4 FRVDSASFTWHSLNLDSQSTVLEFEDSPLAEKSLVARDSGDCGASSNLNCSP 159
>AV409070
Length = 357
Score = 30.0 bits (66), Expect = 0.63
Identities = 22/53 (41%), Positives = 25/53 (46%)
Frame = +2
Query: 66 LSYTPTTTSSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSLSSLSSSTPLLPS 118
L+ +PT SSSLPF SP+ SP SA FS SS P PS
Sbjct: 62 LTTSPTHLSSSLPF------------SPSPSPARISALFSKPPTSSFNPKSPS 184
>BP043430
Length = 493
Score = 30.0 bits (66), Expect = 0.63
Identities = 21/47 (44%), Positives = 25/47 (52%)
Frame = +3
Query: 72 TTSSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSLSSLSSSTPLLPS 118
++SSSL SLSL S L S + S S +F SSLS P L S
Sbjct: 240 SSSSSLNSSLSLPSSSSLLSSSSSSSSSTSLSFPSSSLSLPLPSLSS 380
>BP064526
Length = 533
Score = 30.0 bits (66), Expect = 0.63
Identities = 16/35 (45%), Positives = 21/35 (59%)
Frame = -3
Query: 51 SFSLSTNFPTGPTFKLSYTPTTTSSSLPFSLSLKS 85
+FS+ +FP P+ S TPTTT S PF LK+
Sbjct: 297 NFSIRMSFPPPPSIMASQTPTTT--SFPFIKLLKN 199
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 29.3 bits (64), Expect = 1.1
Identities = 36/117 (30%), Positives = 56/117 (47%)
Frame = +1
Query: 2 KLSLKFQNNNENQQTPQTQIMTAKVPITIFNNPLLSTITATNNYSTSDFSFSLSTNFPTG 61
KL KF +NN N++ ++ +A V ++ L +A+++ S+S S SLS+
Sbjct: 178 KLESKFVHNNTNRR----RLDSANV---VYFFLFLGGCSASSSSSSSSSS-SLSS----- 318
Query: 62 PTFKLSYTPTTTSSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSLSSLSSSTPLLPS 118
S TP+++SSS P S S S S SP S++ SS + P PS
Sbjct: 319 -----SSTPSSSSSSPPSSSSSSSSSSSSSSSWSSPSSSSSSSPFSSGPAPAPGPPS 474
>TC19735 similar to UP|Q8NJM5 (Q8NJM5) Probable ATP-dependent RNA helicase,
partial (5%)
Length = 565
Score = 29.3 bits (64), Expect = 1.1
Identities = 22/78 (28%), Positives = 38/78 (48%)
Frame = +3
Query: 267 EGNLQVLKEMKIDLENVEKENKEMKRVLDEMKMGVSKGKVSKQKHAGESVQSWGSNKNGR 326
E NLQ ++ EN K+ KE K D+ + V+ ++HA E + G+N +
Sbjct: 129 EDNLQ--SSPMVETEN-NKKKKEKKNKHDKKR----PRDVANEEHAEEEEEQIGNNSDRD 287
Query: 327 KENEKKQEIKPQNIVVSD 344
E+ KK++ K + V +
Sbjct: 288 GESSKKKKKKKHKVEVEE 341
>BP055303
Length = 515
Score = 29.3 bits (64), Expect = 1.1
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = -1
Query: 18 QTQIMTAKVPITIFNNPLLSTITATNNYSTSDFSFSLSTNFPTGPTFKLSYTPTTTSSSL 77
QTQ +T + P+ +T T + + STS S+S NFP P+ + + T S +
Sbjct: 419 QTQALTLPSACNVQTPPITTTCTGSASSSTSPTGVSVS-NFPNSPSGSSTVSSWTGGSGI 243
>AV409550
Length = 413
Score = 28.9 bits (63), Expect = 1.4
Identities = 21/56 (37%), Positives = 30/56 (53%), Gaps = 7/56 (12%)
Frame = +3
Query: 66 LSYTPTTTSSSLPFS--LSLKSGLGLFGS-----PNHSPLVFSANFSLSSLSSSTP 114
L+ TPTTTS+S+P ++L S L S P+ SP + + SS SS+P
Sbjct: 210 LTLTPTTTSTSIPLPAIMALTSPAWLTPSETSARPSSSPTASGSALASSSAPSSSP 377
>AV416769
Length = 265
Score = 28.9 bits (63), Expect = 1.4
Identities = 23/54 (42%), Positives = 30/54 (54%), Gaps = 1/54 (1%)
Frame = +2
Query: 105 SLSSLSSSTPLLPSFSLHFKPQFGHFSLHKTVLSDSSNTNPLSVSPQI-EKGFV 157
+L+SLS S+PLLP SL KP +LS + +PLS+S EK FV
Sbjct: 38 TLTSLSLSSPLLPPLSLSLKPFL--------LLSLLTPLSPLSLSRNSPEKEFV 175
>TC13932 similar to UP|Q38901 (Q38901) Cytosolic cyclophilin
(Peptidyl-prolyl cis-trans isomerase) (PPIase)
(Rotamase) , partial (96%)
Length = 580
Score = 28.9 bits (63), Expect = 1.4
Identities = 29/94 (30%), Positives = 41/94 (42%), Gaps = 2/94 (2%)
Frame = -1
Query: 32 NNPLLSTITATNNYSTSDFSFSLSTNFPTGPTFKLSYTPTTTSSSLPFSLSLKSGLGLFG 91
NN STI ++ +T FS L P PTF +S+T S++ P + S G+F
Sbjct: 577 NNFQFSTICPQSSITT--FSDVLP---PPEPTFSISFTTLYPSTTFPNTTCFPSSHGVFA 413
Query: 92 --SPNHSPLVFSANFSLSSLSSSTPLLPSFSLHF 123
N P V F++ + P FSL F
Sbjct: 412 VHMKN*EPFVLGPAFAMERI----PGPVCFSLKF 323
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.310 0.128 0.366
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,440,118
Number of Sequences: 28460
Number of extensions: 108527
Number of successful extensions: 758
Number of sequences better than 10.0: 78
Number of HSP's better than 10.0 without gapping: 726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 749
length of query: 360
length of database: 4,897,600
effective HSP length: 91
effective length of query: 269
effective length of database: 2,307,740
effective search space: 620782060
effective search space used: 620782060
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC144516.4