BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144516.3 + phase: 0
(664 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10797 weakly similar to PIR|C86287|C86287 F9L1.24 protein - Ar... 93 1e-19
AV769427 90 1e-18
BP038206 83 2e-16
AW428655 75 3e-14
AV408976 63 2e-10
BP078300 63 2e-10
BP053178 61 7e-10
TC11222 60 1e-09
AV776899 50 9e-07
TC18596 30 1.2
AW164129 29 2.1
TC15401 UP|CAE45587 (CAE45587) Vacuolar proton-ATPase subunit-li... 29 2.8
TC15402 UP|CAE45587 (CAE45587) Vacuolar proton-ATPase subunit-li... 29 2.8
BP041056 28 3.6
TC9405 homologue to UP|Q84V96 (Q84V96) Aldehyde dehydrogenase 1 ... 28 3.6
BP062378 28 4.7
TC14612 similar to UP|PAL1_SOYBN (P27991) Phenylalanine ammonia-... 28 6.2
AV766852 28 6.2
TC19125 UP|GBA1_LOTJA (P49082) Guanine nucleotide-binding protei... 28 6.2
BP033178 28 6.2
>TC10797 weakly similar to PIR|C86287|C86287 F9L1.24 protein - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (10%)
Length = 657
Score = 93.2 bits (230), Expect = 1e-19
Identities = 43/99 (43%), Positives = 70/99 (70%)
Frame = +3
Query: 551 EFEEYESQSSNNTGKSELDTYLDELRMPLSQEFDVLAFWKERSRRSPNLARMACDILSIP 610
+F+ Y ++S+ KSELD YL+E +P +FDVL +WK + P L++MA DILSIP
Sbjct: 9 DFDAYIMETSSQQMKSELDQYLEESLLPRVPDFDVLGWWKINQVKYPTLSKMARDILSIP 188
Query: 611 ITTVASESAFSIGARVVNRYRSSMKDDSVQALLCARSWL 649
++TV SES F ++ +++YRSS++ ++V+AL+C + W+
Sbjct: 189 VSTVPSESFFDRKSQEMDQYRSSLRPEAVEALVCTQDWM 305
>AV769427
Length = 492
Score = 89.7 bits (221), Expect = 1e-18
Identities = 38/99 (38%), Positives = 70/99 (70%)
Frame = -1
Query: 551 EFEEYESQSSNNTGKSELDTYLDELRMPLSQEFDVLAFWKERSRRSPNLARMACDILSIP 610
+F+ Y ++S+ K ELD YL+E +P +FDVL +WK + P L++MA DIL++P
Sbjct: 474 DFDVYIMETSSQQMKCELDQYLEESLLPRVSDFDVLGWWKLNKIKYPTLSKMARDILAVP 295
Query: 611 ITTVASESAFSIGARVVNRYRSSMKDDSVQALLCARSWL 649
+++V ++S F ++ +++YRSS++ ++V+AL+CA+ W+
Sbjct: 294 VSSVPADSVFDTKSKEMDQYRSSLRPETVEALVCAKDWM 178
>BP038206
Length = 563
Score = 82.8 bits (203), Expect = 2e-16
Identities = 49/162 (30%), Positives = 84/162 (51%), Gaps = 9/162 (5%)
Frame = -3
Query: 78 KLLTCESGSGTSHLKRHVRSC---SKTIKNHDVGEMMIDVEGKLRKKK-----FDPMANR 129
K L+ S +GT+HL+ HV C + HD G+ + + K++ +D ++
Sbjct: 558 KKLSAISKNGTNHLRNHVAKCV*MKIQTRGHDKGQAFLMPKASQGKQELGAGAYDADNSK 379
Query: 130 EFLARIMITHGAPFNMVEWKVFREYQKFLNDDCVFVSRNTIAKEILNVYRDEKQKLKSQL 189
LA +I H P ++V+ F+ Y L +RNTI KE++ +Y +K + L
Sbjct: 378 SQLACAIILHDYPLSIVDHIGFQRYSNSLQPLFQVPTRNTIKKEVIKIYEFQKTCVMKML 199
Query: 190 AQIRGRVCLTSDCWTACSNE-GYISLTAHYVNVNWKLESKIL 230
GRV +TSD WT+ + + GY ++T H ++ W L+S++L
Sbjct: 198 DSHEGRVAITSDMWTSNNQKRGYTTVTTHCIDSTWTLQSRVL 73
>AW428655
Length = 403
Score = 75.5 bits (184), Expect = 3e-14
Identities = 30/92 (32%), Positives = 58/92 (62%)
Frame = +2
Query: 406 MCEILKPFFTITNLISGSSYPTSNLYFGEIWKIECLIRSYLTSEDLLIQKMAENMKVKFD 465
+C LKP + N+++ +++PT+ +F E+WK++ + + +ED I + + M+ K D
Sbjct: 14 VCTYLKPLYDAANILTPTTHPTAITFFHEVWKLQLDMSRAVMNEDPFIGNLTKPMQEKID 193
Query: 466 KYWSDYNVVLAVGAVLDPTKKFNFLKFAYEKL 497
KYW D N++LA+ V+DP K ++F++ K+
Sbjct: 194 KYWRDCNLILALAVVMDPRFKMKLVEFSFTKI 289
>AV408976
Length = 340
Score = 62.8 bits (151), Expect = 2e-10
Identities = 37/101 (36%), Positives = 58/101 (56%), Gaps = 1/101 (0%)
Frame = +2
Query: 235 MEP-PHSGRDLALKVLEMLDDWGIEKKIFSITLDNASANNSMANFLKEHLSLSNSLLLDG 293
MEP P S ++ V L DW IE ++FSIT ++A + +++ N L+ LS+ N L+L+G
Sbjct: 20 MEPYPDSDSAISHAVAVCLSDWNIEGRLFSITCNHALSESALGN-LRSLLSVKNPLILNG 196
Query: 294 EFFHIRCSAHILNLIVQDGLKVVSDALHKIRQSVAYVRVTE 334
+ C A L+ + D L V + KIR SV +V+ +E
Sbjct: 197 QLLVGHCIARTLSNVANDLLSAVEGIVKKIRDSVKFVKTSE 319
>BP078300
Length = 401
Score = 62.8 bits (151), Expect = 2e-10
Identities = 28/47 (59%), Positives = 36/47 (76%)
Frame = -2
Query: 603 ACDILSIPITTVASESAFSIGARVVNRYRSSMKDDSVQALLCARSWL 649
A DIL+IPI+TVASES FS RV+N YRS + D+S++AL+C WL
Sbjct: 400 AADILAIPISTVASESTFSA*GRVINEYRSKLNDESIEALICGGDWL 260
>BP053178
Length = 529
Score = 60.8 bits (146), Expect = 7e-10
Identities = 29/57 (50%), Positives = 38/57 (65%)
Frame = -1
Query: 595 RSPNLARMACDILSIPITTVASESAFSIGARVVNRYRSSMKDDSVQALLCARSWLHG 651
R P L+R+A DIL IP++TVASESAFS G RV+N +RSS+ +C W+ G
Sbjct: 529 RLPMLSRIARDILXIPVSTVASESAFSTGGRVLNDFRSSLTPKMADMSICTPDWMKG 359
>TC11222
Length = 557
Score = 59.7 bits (143), Expect = 1e-09
Identities = 26/47 (55%), Positives = 39/47 (82%)
Frame = +2
Query: 602 MACDILSIPITTVASESAFSIGARVVNRYRSSMKDDSVQALLCARSW 648
+A D+L++P++TVASESAFS RV+N YRSS+ +V+AL+CA++W
Sbjct: 5 IAKDVLAMPVSTVASESAFSTRGRVLNNYRSSLSPKTVEALVCAQNW 145
>AV776899
Length = 396
Score = 50.4 bits (119), Expect = 9e-07
Identities = 25/79 (31%), Positives = 39/79 (48%), Gaps = 1/79 (1%)
Frame = +2
Query: 23 NAQEVSSDPKDKKRKGKAKAKDKSKGKALTSDVWLYLVKVGIVD-GVEKCRCKACHKLLT 81
N D ++++ + K + KALTSDVW +G D G+++ +C C
Sbjct: 158 NVDADMEDEENEEPESLVLEKGTKRPKALTSDVWQKFTIIGRGDDGIQRAKCNGCRSEYK 337
Query: 82 CESGSGTSHLKRHVRSCSK 100
C GTS L+RH + C+K
Sbjct: 338 CGGDYGTSTLRRHAKVCTK 394
>TC18596
Length = 577
Score = 30.0 bits (66), Expect = 1.2
Identities = 21/63 (33%), Positives = 33/63 (52%), Gaps = 7/63 (11%)
Frame = +1
Query: 416 ITNLISGSSYPTS---NLYFGEIWKIECLIRSYLTSEDLLI----QKMAENMKVKFDKYW 468
+T +I SY S N +FG I+CLIRS T++ +I ++ A +K+K K
Sbjct: 94 LTKIIKRISYVESKCKNYHFGHQMVIDCLIRSRETTKPFVIIPSLRRPATKIKIKIKKED 273
Query: 469 SDY 471
D+
Sbjct: 274 MDH 282
>AW164129
Length = 372
Score = 29.3 bits (64), Expect = 2.1
Identities = 15/43 (34%), Positives = 23/43 (52%), Gaps = 3/43 (6%)
Frame = -2
Query: 45 KSKGKALTSDVWLYLVKVGIVDGVEKCRCK---ACHKLLTCES 84
+S L S+ + V +VDG E C+C+ +CH LL +S
Sbjct: 227 QSHDPPLKSEALSFNFSVKVVDGSEGCKCRCSWSCHPLLLTDS 99
>TC15401 UP|CAE45587 (CAE45587) Vacuolar proton-ATPase subunit-like protein,
partial (86%)
Length = 2417
Score = 28.9 bits (63), Expect = 2.8
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -3
Query: 639 VQALLCARSWLHGFEGISSIIFTI 662
+QALLCAR W GF +SS + +
Sbjct: 1698 LQALLCARKWRIGFCTVSSFVLLL 1627
>TC15402 UP|CAE45587 (CAE45587) Vacuolar proton-ATPase subunit-like protein,
complete
Length = 2423
Score = 28.9 bits (63), Expect = 2.8
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -3
Query: 639 VQALLCARSWLHGFEGISSIIFTI 662
+QALLCAR W GF +SS + +
Sbjct: 2034 LQALLCARKWRIGFCTVSSFVLLL 1963
>BP041056
Length = 298
Score = 28.5 bits (62), Expect = 3.6
Identities = 10/29 (34%), Positives = 18/29 (61%)
Frame = -2
Query: 620 FSIGARVVNRYRSSMKDDSVQALLCARSW 648
F R ++ YRSS+ +++AL+C + W
Sbjct: 294 FDTEIRKMDSYRSSLDSPTLEALICTKDW 208
>TC9405 homologue to UP|Q84V96 (Q84V96) Aldehyde dehydrogenase 1 precursor
, partial (73%)
Length = 1249
Score = 28.5 bits (62), Expect = 3.6
Identities = 11/26 (42%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
Frame = +1
Query: 200 SDCW-TACSNEGYISLTAHYVNVNWK 224
SDCW T+C + G + H++N WK
Sbjct: 862 SDCWCTSCKSHGCGQASIHWIN*YWK 939
>BP062378
Length = 342
Score = 28.1 bits (61), Expect = 4.7
Identities = 13/32 (40%), Positives = 23/32 (71%)
Frame = -1
Query: 427 TSNLYFGEIWKIECLIRSYLTSEDLLIQKMAE 458
++NLY I+ ECL+R++ S+D +I+K+ E
Sbjct: 210 SNNLYMKFIFHEECLLRNFF-SDDTVIKKLCE 118
>TC14612 similar to UP|PAL1_SOYBN (P27991) Phenylalanine ammonia-lyase 1 ,
partial (43%)
Length = 1049
Score = 27.7 bits (60), Expect = 6.2
Identities = 14/37 (37%), Positives = 22/37 (58%), Gaps = 2/37 (5%)
Frame = +3
Query: 515 KLFSEYIKNGIPSNLSSSQVQPSYGG--GTRITSSSY 549
+L ++ NG+PSNL++S+ Y G G I +SY
Sbjct: 66 ELVDDHYNNGLPSNLTASRNPSLYYGFKGAEIAMASY 176
>AV766852
Length = 492
Score = 27.7 bits (60), Expect = 6.2
Identities = 18/66 (27%), Positives = 30/66 (45%)
Frame = +2
Query: 155 QKFLNDDCVFVSRNTIAKEILNVYRDEKQKLKSQLAQIRGRVCLTSDCWTACSNEGYISL 214
Q+FL V + N I KE+ D+ Q K Q+ Q++ C A ++ +
Sbjct: 299 QRFLRPVLVHLGNNQIDKEV-----DQAQNGKEQVEQVQMVSVDIVPC*PASTSSNILGC 463
Query: 215 TAHYVN 220
T HY++
Sbjct: 464 TIHYLD 481
>TC19125 UP|GBA1_LOTJA (P49082) Guanine nucleotide-binding protein alpha-1
subunit (GP-alpha-1), complete
Length = 1597
Score = 27.7 bits (60), Expect = 6.2
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = +2
Query: 646 RSWLHGFEGISSIIF 660
R W+H FEG+S++IF
Sbjct: 692 RKWIHLFEGVSAVIF 736
>BP033178
Length = 524
Score = 27.7 bits (60), Expect = 6.2
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +2
Query: 292 DGEFFHIRCSAHILNLIVQD 311
DG++FH+ CS +IL L ++D
Sbjct: 329 DGKYFHV*CSGYILILHIRD 388
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.134 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,807,854
Number of Sequences: 28460
Number of extensions: 162484
Number of successful extensions: 842
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 837
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 839
length of query: 664
length of database: 4,897,600
effective HSP length: 96
effective length of query: 568
effective length of database: 2,165,440
effective search space: 1229969920
effective search space used: 1229969920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144516.3