BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144516.12 - phase: 0
(105 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11633 27 0.70
TC14260 weakly similar to UP|AAR24673 (AAR24673) At5g62200, part... 26 0.91
TC16750 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehyd... 26 0.91
TC12503 homologue to UP|Q9ATD9 (Q9ATD9) BNLGHi233, partial (24%) 26 1.2
TC18417 weakly similar to UP|Q8W228 (Q8W228) Cytochrome P450, pa... 25 1.6
TC14630 25 2.0
TC19458 weakly similar to UP|Q9STE8 (Q9STE8) Chloroplast import-... 25 2.6
AV776172 23 5.9
CB827758 23 5.9
TC11563 23 5.9
TC8023 23 5.9
TC10541 similar to UP|CYSL_CAPAN (P31300) Cysteine synthase, chl... 23 5.9
AW720360 23 7.7
TC16292 similar to UP|O49260 (O49260) G5bf protein, partial (46%) 23 7.7
>TC11633
Length = 436
Score = 26.6 bits (57), Expect = 0.70
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1
Query: 63 SVAASNDKVTLAGYCPVSEDLEPCR 87
S + SND TL+ PV+E PCR
Sbjct: 97 SPSPSNDSPTLSDSSPVAESTVPCR 23
>TC14260 weakly similar to UP|AAR24673 (AAR24673) At5g62200, partial (51%)
Length = 788
Score = 26.2 bits (56), Expect = 0.91
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -3
Query: 71 VTLAGYCPVSEDLEPCRWEILP 92
+TL+G P+ DL RW+ILP
Sbjct: 471 ITLSGTQPLDPDLYR*RWQILP 406
>TC16750 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehydrogenase
(CAD) , partial (34%)
Length = 442
Score = 26.2 bits (56), Expect = 0.91
Identities = 12/38 (31%), Positives = 19/38 (49%)
Frame = -3
Query: 13 QPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKR 50
+P +S T N + S+ PTT PG G +++ R
Sbjct: 347 RPAPTISPTVNLVTSEPTSSTSPTTSCPGTMG*LDMPR 234
>TC12503 homologue to UP|Q9ATD9 (Q9ATD9) BNLGHi233, partial (24%)
Length = 957
Score = 25.8 bits (55), Expect = 1.2
Identities = 13/36 (36%), Positives = 16/36 (44%)
Frame = -1
Query: 53 NASVSVFEFGSVAASNDKVTLAGYCPVSEDLEPCRW 88
N S+ F V A + + TL G C S CRW
Sbjct: 750 NCSILSSFFNMVGAGHREETLEGSCARSS*CSDCRW 643
>TC18417 weakly similar to UP|Q8W228 (Q8W228) Cytochrome P450, partial (38%)
Length = 747
Score = 25.4 bits (54), Expect = 1.6
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = -3
Query: 63 SVAASNDKVTLAGYCPVSEDL 83
S AAS ++ T+AG CP +D+
Sbjct: 292 SFAASGEEATMAGTCPSFKDM 230
>TC14630
Length = 617
Score = 25.0 bits (53), Expect = 2.0
Identities = 14/44 (31%), Positives = 23/44 (51%), Gaps = 1/44 (2%)
Frame = -1
Query: 62 GSVAASNDK-VTLAGYCPVSEDLEPCRWEILPAVESNAPQFRVV 104
GS N++ V +G+C + +L RW + P S++ F VV
Sbjct: 362 GSTEPGNNRCVVKSGFC**NPELSATRWVLTPL*TSSSAVFLVV 231
>TC19458 weakly similar to UP|Q9STE8 (Q9STE8) Chloroplast import-associated
channel homolog (AT3g46740/T6H20_230), partial (10%)
Length = 469
Score = 24.6 bits (52), Expect = 2.6
Identities = 14/49 (28%), Positives = 24/49 (48%), Gaps = 8/49 (16%)
Frame = -3
Query: 7 RKGKNSQPEAKLS--------TTENAAKNGGASAVKPTTEAPGMNGAVE 47
R+G+ S PEA +S T+ +++ G +++ E G G VE
Sbjct: 326 RRGEESGPEASISVASAAAEPTSTSSSAGGASTSASARGEEGGERGGVE 180
>AV776172
Length = 433
Score = 23.5 bits (49), Expect = 5.9
Identities = 11/25 (44%), Positives = 12/25 (48%)
Frame = +1
Query: 14 PEAKLSTTENAAKNGGASAVKPTTE 38
P A STT A G + PTTE
Sbjct: 316 PPASRSTTRTRASPGTGTRTSPTTE 390
>CB827758
Length = 485
Score = 23.5 bits (49), Expect = 5.9
Identities = 11/34 (32%), Positives = 15/34 (43%)
Frame = +2
Query: 7 RKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAP 40
R+G + T AK G S+ KPT +P
Sbjct: 50 RRG*RRSKRRSSTATSQ*AKRSGLSSTKPTRNSP 151
>TC11563
Length = 470
Score = 23.5 bits (49), Expect = 5.9
Identities = 7/16 (43%), Positives = 11/16 (68%)
Frame = +2
Query: 86 CRWEILPAVESNAPQF 101
C WE+L ++ + PQF
Sbjct: 47 CTWELLSYIQDSFPQF 94
>TC8023
Length = 1089
Score = 23.5 bits (49), Expect = 5.9
Identities = 15/57 (26%), Positives = 24/57 (41%), Gaps = 4/57 (7%)
Frame = +3
Query: 8 KGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKN----ASVSVFE 60
KGK Q EAK + K+ G + + A+E+ + N ++S FE
Sbjct: 249 KGKTKQAEAKQGQPSKSKKSKGEKGKCLISSSSACQVAMEIAKSPNRQD*VTISTFE 419
>TC10541 similar to UP|CYSL_CAPAN (P31300) Cysteine synthase, chloroplast
precursor (O-acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) (CSase B) (CS-B) (OAS-TL
B) , partial (25%)
Length = 568
Score = 23.5 bits (49), Expect = 5.9
Identities = 22/73 (30%), Positives = 32/73 (43%), Gaps = 4/73 (5%)
Frame = +3
Query: 18 LSTTENAAKNGG----ASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAASNDKVTL 73
LSTT + A +VKP TE G+N A +V + + V+ V K ++
Sbjct: 249 LSTTTRVSSPSSVVCKAVSVKPPTEIEGLNIADDVTQLIGKTPMVYLNNIV-----KGSV 413
Query: 74 AGYCPVSEDLEPC 86
A E +EPC
Sbjct: 414 ANIAAKLEIMEPC 452
>AW720360
Length = 544
Score = 23.1 bits (48), Expect = 7.7
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +1
Query: 24 AAKNGGASAVKPTTEAPG 41
AA N ASA+ P T +PG
Sbjct: 391 AAANSAASAMVPLTPSPG 444
>TC16292 similar to UP|O49260 (O49260) G5bf protein, partial (46%)
Length = 671
Score = 23.1 bits (48), Expect = 7.7
Identities = 8/18 (44%), Positives = 13/18 (71%)
Frame = +1
Query: 82 DLEPCRWEILPAVESNAP 99
+L+PC +LP +ES +P
Sbjct: 37 ELQPC*RVVLPQIESRSP 90
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.127 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,762,005
Number of Sequences: 28460
Number of extensions: 16414
Number of successful extensions: 102
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 102
length of query: 105
length of database: 4,897,600
effective HSP length: 81
effective length of query: 24
effective length of database: 2,592,340
effective search space: 62216160
effective search space used: 62216160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 47 (22.7 bits)
Medicago: description of AC144516.12