BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144515.9 + phase: 0
(207 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP065719 39 5e-04
TC17405 similar to UP|Q940D1 (Q940D1) At1g64110/F22C12_22, parti... 32 0.079
TC14279 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycop... 32 0.079
AU251550 31 0.13
TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycop... 31 0.13
AV407673 29 0.51
BP085994 29 0.67
AV427333 28 0.87
TC17551 weakly similar to UP|AAR24662 (AAR24662) At5g26960, part... 25 1.1
TC10164 weakly similar to UP|O24099 (O24099) MtN12 protein (Frag... 28 1.1
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 28 1.5
TC12772 UP|Q9LMV5 (Q9LMV5) F5M15.20, partial (13%) 27 1.9
AU251673 27 1.9
AW164126 27 1.9
BP071106 27 1.9
AV427911 27 2.5
AV426322 27 3.3
CN825388 27 3.3
BP043633 27 3.3
TC8260 similar to UP|Q94ES8 (Q94ES8) Nodule extensin (Fragment),... 27 3.3
>BP065719
Length = 567
Score = 39.3 bits (90), Expect = 5e-04
Identities = 22/76 (28%), Positives = 39/76 (50%)
Frame = +3
Query: 2 EDAAEWYTSLSKDDVHTFDELAAAFKSHYGFNTRLKPNREFLRSLSQKKEESFREYAQRW 61
++A W+T+L+ VHT+ +L F + F K + + L S+ +K ES +Y R+
Sbjct: 315 KNAFTWFTTLAPRSVHTWAQLERIFHEQF-FRGECKVSXKDLASVKRKPAESIDDYLNRF 491
Query: 62 RGAAARITPALDEEEM 77
R +R + E E+
Sbjct: 492 RMLKSRCFTHVSEHEL 539
>TC17405 similar to UP|Q940D1 (Q940D1) At1g64110/F22C12_22, partial (10%)
Length = 522
Score = 32.0 bits (71), Expect = 0.079
Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 4/41 (9%)
Frame = +3
Query: 164 INAAQMPPSYPYVPYS----QHPFFPPFYHQYPLPPGQNSL 200
I A +PP YP P+S Q+P F +Q+ +P G+ SL
Sbjct: 57 IAAISLPPQYPVYPFSLNLLQNPLNHSFSYQFLIPFGEKSL 179
>TC14279 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycoprotein
(HRGP) (Fragment), partial (57%)
Length = 941
Score = 32.0 bits (71), Expect = 0.079
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 170 PPSYPYVPYSQHPFFPPFYHQYPLPPGQNSLFPFYF 205
PP P Y + P PP+Y++ P PP + P+Y+
Sbjct: 31 PPPVPKPYYYKSPPPPPYYYKSPPPPSPSPPPPYYY 138
Score = 30.0 bits (66), Expect = 0.30
Identities = 14/38 (36%), Positives = 20/38 (51%), Gaps = 2/38 (5%)
Frame = +1
Query: 170 PPSYPYV--PYSQHPFFPPFYHQYPLPPGQNSLFPFYF 205
PP Y Y P H PP+Y++ P PP + P+Y+
Sbjct: 121 PPPYYYQSPPPPSHSPPPPYYYKSPPPPSPSPPPPYYY 234
Score = 27.7 bits (60), Expect = 1.5
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = +1
Query: 170 PPSYPYV---PYSQHPFFPPFYHQYPLPPGQNSLFPFYF 205
PP Y Y P S P PP+Y++ P PP + P+Y+
Sbjct: 169 PPPYYYKSPPPPSPSPP-PPYYYKSPPPPSPSPPPPYYY 282
Score = 26.2 bits (56), Expect = 4.3
Identities = 15/39 (38%), Positives = 20/39 (50%), Gaps = 3/39 (7%)
Frame = +1
Query: 170 PPSYPYV---PYSQHPFFPPFYHQYPLPPGQNSLFPFYF 205
PP Y Y P S P PP+Y++ P PP P+Y+
Sbjct: 217 PPPYYYKSPPPPSPSPP-PPYYYKSPPPPSPIPHPPYYY 330
>AU251550
Length = 329
Score = 31.2 bits (69), Expect = 0.13
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = +2
Query: 170 PPSYPYVPYSQHPFFPPFYHQYPLPP 195
PP P PY+ P PPF H +P PP
Sbjct: 140 PPGPPRDPYAPAPPPPPFGHHHPPPP 217
>TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycoprotein
DZ-HRGP precursor, partial (8%)
Length = 584
Score = 31.2 bits (69), Expect = 0.13
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = +1
Query: 162 APINAAQMPPSYPYVPYSQHPFFPPFYHQYPLPP 195
AP + P YP +PY QH F+PP P PP
Sbjct: 172 APPYGMEFSP-YPSLPYPQHQFYPPPMAPPPPPP 270
>AV407673
Length = 427
Score = 29.3 bits (64), Expect = 0.51
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -3
Query: 171 PSYPYVPYSQHPFFPPFYHQY 191
P +P +HPFFPPF+ Y
Sbjct: 197 PLFPTFQNYRHPFFPPFFFSY 135
>BP085994
Length = 377
Score = 28.9 bits (63), Expect = 0.67
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +1
Query: 169 MPPSYPYVPYSQHPFFPPFYHQYPLP 194
+PP YP VP+ HP F H +PLP
Sbjct: 145 IPPPYPAVPF*LHPIFSSRPH-HPLP 219
>AV427333
Length = 387
Score = 28.5 bits (62), Expect = 0.87
Identities = 15/33 (45%), Positives = 17/33 (51%)
Frame = +3
Query: 170 PPSYPYVPYSQHPFFPPFYHQYPLPPGQNSLFP 202
PPS P P S HPF PP H P P + + P
Sbjct: 225 PPSSPPPP-SSHPFTPPPPHVRPPPSPHHPITP 320
>TC17551 weakly similar to UP|AAR24662 (AAR24662) At5g26960, partial (22%)
Length = 505
Score = 24.6 bits (52), Expect(2) = 1.1
Identities = 11/24 (45%), Positives = 13/24 (53%)
Frame = +1
Query: 179 SQHPFFPPFYHQYPLPPGQNSLFP 202
S H F PP H LP + +LFP
Sbjct: 118 SHHHFLPP*RHHSRLPHPRPTLFP 189
Score = 21.9 bits (45), Expect(2) = 1.1
Identities = 10/27 (37%), Positives = 13/27 (48%)
Frame = +3
Query: 161 VAPINAAQMPPSYPYVPYSQHPFFPPF 187
V+PI PP P P ++ PPF
Sbjct: 51 VSPIQTTPPPPPPPPSPNHRNNLPPPF 131
>TC10164 weakly similar to UP|O24099 (O24099) MtN12 protein (Fragment),
partial (66%)
Length = 566
Score = 28.1 bits (61), Expect = 1.1
Identities = 14/37 (37%), Positives = 18/37 (47%), Gaps = 4/37 (10%)
Frame = +3
Query: 163 PINAAQMPPSYPYVPYSQHPFF----PPFYHQYPLPP 195
PI+ + PP Y PY +P F PPF + PP
Sbjct: 66 PISHSPPPPGQTYPPYIPNPIFLSPPPPFPISHSPPP 176
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 27.7 bits (60), Expect = 1.5
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -1
Query: 163 PINAAQMPPSYPYVPYSQHPFFPPFYHQYPLPP 195
P+ + PPS PY P + P PP YP PP
Sbjct: 527 PLP*REPPPSPPYPPSRRPP*PPPPPPPYPPPP 429
>TC12772 UP|Q9LMV5 (Q9LMV5) F5M15.20, partial (13%)
Length = 465
Score = 27.3 bits (59), Expect = 1.9
Identities = 15/34 (44%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Frame = +2
Query: 40 REFLRSLSQ-KKEESFREYAQRWRGAAARITPAL 72
REFL SL + KKE + + A GAAAR++ ++
Sbjct: 53 REFLSSLRERKKEREYHDNAIESGGAAARVSDSI 154
>AU251673
Length = 413
Score = 27.3 bits (59), Expect = 1.9
Identities = 25/116 (21%), Positives = 56/116 (47%), Gaps = 6/116 (5%)
Frame = +2
Query: 4 AAEWYTSLSK-----DDVHTFDELAAAFKSHY-GFNTRLKPNREFLRSLSQKKEESFREY 57
A +WY L++ T+ + +A F + + + R R+F R L Q + + EY
Sbjct: 56 ARDWYNVLTRAKPVGSPPWTWADFSAEFMNRFLPQSVRDGFVRDFER-LEQAEGMTVSEY 232
Query: 58 AQRWRGAAARITPALDEEEMTQTFLKTLKKDYVERMIIAAPNNFSEMVTMGTRLEE 113
+ + + + L EEE + F++ LK+ + ++ + + SE++++ +E+
Sbjct: 233 SAHFTHLSRYVPYPLLEEERVKRFVRGLKEYLFKSVVGSKSSTLSEVLSLALLVEQ 400
>AW164126
Length = 387
Score = 27.3 bits (59), Expect = 1.9
Identities = 14/44 (31%), Positives = 21/44 (46%), Gaps = 1/44 (2%)
Frame = +2
Query: 163 PINAAQMPPSYPYVPYSQHPF-FPPFYHQYPLPPGQNSLFPFYF 205
P + + PP Y P P PP+Y++ P PP P+Y+
Sbjct: 194 PPSPSPPPPYYYKSPPPPSPVPHPPYYYKSPPPPSPIPHPPYYY 325
Score = 25.4 bits (54), Expect = 7.4
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = +2
Query: 171 PSYPYVPYSQHPFFPPFYHQYPLPPGQNSLFPFYF 205
P Y P P P+Y++ P PP + P+Y+
Sbjct: 125 PYYYKSPPPPSPSPKPYYYKSPPPPSPSPPPPYYY 229
Score = 25.4 bits (54), Expect = 7.4
Identities = 12/36 (33%), Positives = 17/36 (46%)
Frame = +2
Query: 170 PPSYPYVPYSQHPFFPPFYHQYPLPPGQNSLFPFYF 205
PP P P PP+Y++ P PP P+Y+
Sbjct: 191 PPPSPSPP-------PPYYYKSPPPPSPVPHPPYYY 277
>BP071106
Length = 402
Score = 27.3 bits (59), Expect = 1.9
Identities = 10/25 (40%), Positives = 13/25 (52%)
Frame = +3
Query: 170 PPSYPYVPYSQHPFFPPFYHQYPLP 194
P +PY+ QHP PP+ P P
Sbjct: 276 PAIHPYIWGGQHPLIPPYGTPVPYP 350
>AV427911
Length = 348
Score = 26.9 bits (58), Expect = 2.5
Identities = 13/41 (31%), Positives = 18/41 (43%)
Frame = +1
Query: 162 APINAAQMPPSYPYVPYSQHPFFPPFYHQYPLPPGQNSLFP 202
+P + P YP P H + P YH P PP + +P
Sbjct: 193 SPPPPPKKPYKYPSPPPPVHVYPKPTYHSPPPPPKKPYKYP 315
>AV426322
Length = 323
Score = 26.6 bits (57), Expect = 3.3
Identities = 12/31 (38%), Positives = 19/31 (60%)
Frame = +3
Query: 17 HTFDELAAAFKSHYGFNTRLKPNREFLRSLS 47
HTF L+++F + + FN L+ FLR L+
Sbjct: 72 HTFSHLSSSFTNTFPFNFSLQTILHFLRLLA 164
>CN825388
Length = 721
Score = 26.6 bits (57), Expect = 3.3
Identities = 19/65 (29%), Positives = 26/65 (39%), Gaps = 1/65 (1%)
Frame = +1
Query: 140 HKKKETEVGMVSAGAGQSMATVAPINAAQMPPSYPYVPYSQHPFF-PPFYHQYPLPPGQN 198
H+ E +V S A + P + Q+PP + P+F P YP P
Sbjct: 76 HEGSNREGSIVKCSLSSSSAHLFPFSCLQLPPPLVLLQGPPKPYFLIPISFPYP-NPLLY 252
Query: 199 SLFPF 203
LFPF
Sbjct: 253 LLFPF 267
>BP043633
Length = 542
Score = 26.6 bits (57), Expect = 3.3
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +1
Query: 170 PPSYPYVPYSQHPFFPPFYH 189
PP P P+ HP FPP H
Sbjct: 469 PPPPPRPPHPPHPQFPPHSH 528
>TC8260 similar to UP|Q94ES8 (Q94ES8) Nodule extensin (Fragment), partial
(79%)
Length = 1017
Score = 26.6 bits (57), Expect = 3.3
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3
Query: 170 PPSYPYVPYSQHPFFPPFYHQYPLP 194
PP P+ PY ++P PP H+YP P
Sbjct: 267 PPPPPHKPY-KYPSPPPPPHKYPHP 338
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.132 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,443,965
Number of Sequences: 28460
Number of extensions: 44312
Number of successful extensions: 455
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 425
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 452
length of query: 207
length of database: 4,897,600
effective HSP length: 86
effective length of query: 121
effective length of database: 2,450,040
effective search space: 296454840
effective search space used: 296454840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144515.9