BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144513.9 + phase: 0
(261 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14653 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lec... 165 6e-42
AV423736 158 1e-39
TC9848 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lect... 128 9e-31
TC8152 101 1e-22
TC18680 81 2e-16
TC16811 63 6e-11
TC9742 similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin PP2-... 62 1e-10
TC8955 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lect... 57 4e-09
TC13194 weakly similar to UP|Q94EW1 (Q94EW1) Nictaba, partial (29%) 50 3e-07
AV772932 48 2e-06
AV423946 40 5e-04
CB828206 39 7e-04
TC8893 32 0.14
BP048367 31 0.25
AV771384 28 2.1
BP034385 28 2.1
TC13195 28 2.1
AV423739 27 2.7
AV415704 27 4.6
AW163944 26 6.1
>TC14653 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like
protein, partial (45%)
Length = 643
Score = 165 bits (418), Expect = 6e-42
Identities = 90/181 (49%), Positives = 117/181 (63%), Gaps = 6/181 (3%)
Frame = +2
Query: 2 ELLPEDCFAHILSFTSPLDVCRVSLLSSVVQSMADSDFLWEKFLPHKYQEIISR-----L 56
E LPE C A+ILS TSPLDV R++L+SS +S ADSD +W++FLP + IIS
Sbjct: 107 ENLPEGCIANILSLTSPLDVGRLALVSSAFRSAADSDAVWDRFLPDDFNSIISHSSSTAA 286
Query: 57 VDPPLPCSSKKELFARLC-KPSLIDDGNKMFSIEKTTGKICYLLSARQLSITFGNTSLYW 115
D SKK+L+ L P +I DG K F + GK CY+LSAR LSI +G+T YW
Sbjct: 287 ADSSSSFKSKKDLYLSLSHNPLIIGDGKKSFQL--VNGKKCYMLSARALSIVWGDTPRYW 460
Query: 116 SWKQVQGSRFAEAAELRTICWLEIKGSINSEMLSPKTMYGAYLKVKIADRAYGLDSLPSE 175
W + +RF+E AEL ++CWLEI+G IN++MLSP+TMYGAYL K + AYG + E
Sbjct: 461 RWISLPEARFSEVAELVSVCWLEIRGWINNKMLSPETMYGAYLVFKPSGGAYGFEFQSVE 640
Query: 176 V 176
V
Sbjct: 641 V 643
>AV423736
Length = 454
Score = 158 bits (399), Expect = 1e-39
Identities = 77/139 (55%), Positives = 99/139 (70%), Gaps = 1/139 (0%)
Frame = +2
Query: 4 LPEDCFAHILSFTSPLDVCRVSLLSSVVQSMADSDFLWEKFLPHKYQEIISRLVDPP-LP 62
LPEDC + ILS TSP D CR SL+SS ++S ADSD +W FLP Y++I+SR V+P L
Sbjct: 38 LPEDCVSEILSHTSPPDACRFSLVSSTLRSAADSDMVWRSFLPSDYEDIVSRAVNPSALQ 217
Query: 63 CSSKKELFARLCKPSLIDDGNKMFSIEKTTGKICYLLSARQLSITFGNTSLYWSWKQVQG 122
SS K+LF LC P L+D GNK F ++K +GK Y+LSAR LSIT+ + ++WSW+
Sbjct: 218 FSSYKQLFHALCSPLLLDGGNKSFKLDKLSGKKSYILSARDLSITWSSDPMFWSWRSNPE 397
Query: 123 SRFAEAAELRTICWLEIKG 141
SRF E AELRT+ WLEI+G
Sbjct: 398 SRFREVAELRTVNWLEIEG 454
>TC9848 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin
PP2-like protein, partial (19%)
Length = 394
Score = 128 bits (322), Expect = 9e-31
Identities = 62/120 (51%), Positives = 84/120 (69%), Gaps = 2/120 (1%)
Frame = +1
Query: 1 MELLPEDCFAHILSFTSPLDVCRVSLLSSVVQSMADSDFLWEKFLPHKYQEIISRLVDPP 60
+E LPE+C + ILS TSP D CR S+LSS ++S A+SD LW FLP Y +IISR ++P
Sbjct: 31 IETLPEECVSEILSHTSPPDACRFSMLSSTLRSAANSDMLWRSFLPSDYSDIISRALNPL 210
Query: 61 L--PCSSKKELFARLCKPSLIDDGNKMFSIEKTTGKICYLLSARQLSITFGNTSLYWSWK 118
SS K+LF LC P L+D G +F ++K++GK Y+LSAR+LSIT+ + LYW+WK
Sbjct: 211 FLNSSSSFKDLFKALCNPLLLDGGTMIFKLDKSSGKKSYILSARELSITWSSDPLYWTWK 390
>TC8152
Length = 715
Score = 101 bits (251), Expect = 1e-22
Identities = 52/165 (31%), Positives = 88/165 (52%)
Frame = +1
Query: 4 LPEDCFAHILSFTSPLDVCRVSLLSSVVQSMADSDFLWEKFLPHKYQEIISRLVDPPLPC 63
LPE C A IL + P +C+++ L+ ++ + +DF+WE LP Y+ I+ ++
Sbjct: 199 LPESCVALILGYADPPQICQLATLNRAFRAASSADFVWESKLPANYRAIVRKIFTDFPSD 378
Query: 64 SSKKELFARLCKPSLIDDGNKMFSIEKTTGKICYLLSARQLSITFGNTSLYWSWKQVQGS 123
SSK++ +A LC+ + +D G K ++K+TGKIC LS + LSIT + YW + S
Sbjct: 379 SSKRDTYAALCRLNTLDGGTKKAWLDKSTGKICMCLSFKGLSITGIDDRRYWKDIHTEES 558
Query: 124 RFAEAAELRTICWLEIKGSINSEMLSPKTMYGAYLKVKIADRAYG 168
RF A L+ W ++ G + E P Y + ++ + + G
Sbjct: 559 RFGTVAYLQQTWWFQVDGEV--EFPFPAGTYSLFFRLHLGRASKG 687
>TC18680
Length = 492
Score = 80.9 bits (198), Expect = 2e-16
Identities = 36/104 (34%), Positives = 62/104 (59%)
Frame = +1
Query: 4 LPEDCFAHILSFTSPLDVCRVSLLSSVVQSMADSDFLWEKFLPHKYQEIISRLVDPPLPC 63
+PE+C A + +P ++C ++ L+ + A +D +WE LP Y++++ L
Sbjct: 181 IPENCVARVFLHLTPPEICNLARLNRAFRGAASADSVWETKLPSNYKDLLHALPPERFQN 360
Query: 64 SSKKELFARLCKPSLIDDGNKMFSIEKTTGKICYLLSARQLSIT 107
SKK++FA L +P DDGNK +++ TG++C +SA+ LSIT
Sbjct: 361 LSKKDIFALLSRPQPFDDGNKEVWLDRVTGRVCMSISAKALSIT 492
>TC16811
Length = 509
Score = 62.8 bits (151), Expect = 6e-11
Identities = 25/92 (27%), Positives = 57/92 (61%), Gaps = 1/92 (1%)
Frame = +2
Query: 4 LPEDCFAHILSFTSPLDVCRVSLLSSVVQSMADSDFLWEKFLPHKYQEIISRLV-DPPLP 62
+PE+C + +L P ++C+++ ++ + +DFLWE LP Y+ ++++++ + L
Sbjct: 233 VPENCISSLLMSLDPQEICKLARVNKAFHRASSADFLWESKLPPGYKFLVNKVLGEEKLG 412
Query: 63 CSSKKELFARLCKPSLIDDGNKMFSIEKTTGK 94
+KKE++A+LC+P+ D G K +++ + +
Sbjct: 413 SMTKKEIYAKLCQPNFFDGGAKEIWLDRCSAQ 508
>TC9742 similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin PP2-like
protein, partial (20%)
Length = 794
Score = 61.6 bits (148), Expect = 1e-10
Identities = 43/123 (34%), Positives = 66/123 (52%), Gaps = 23/123 (18%)
Frame = +1
Query: 158 LKVKIADRAYGLDSLPSEVSIEVGN-YKSQENVYIRSQSKRNG-KAYCHCNCEH------ 209
L + ++ R YGLDS PSEVS V N + VY+ +++++N + Y H
Sbjct: 235 LIMNVSHRGYGLDSAPSEVSSTVANNVMHTKKVYLWNKNEKNMCEKYTLFRGFHRDVARE 414
Query: 210 ---------------NDEWLEIKLGSFYTEKVQVQEVRMCLKEVKGVHLKGGLIVDGIEL 254
+D W+EI++G F+ + +EV++ + EV G LKGGLIV+GIE+
Sbjct: 415 TLIIQDQINSGPLKRDDGWMEIEVGEFFCDGEIDEEVKVSVMEV-GYQLKGGLIVEGIEV 591
Query: 255 RPK 257
RPK
Sbjct: 592 RPK 600
Score = 40.8 bits (94), Expect = 2e-04
Identities = 16/33 (48%), Positives = 27/33 (81%)
Frame = +2
Query: 84 KMFSIEKTTGKICYLLSARQLSITFGNTSLYWS 116
++F ++K++GK Y+LSAR+LSIT+ + LYW+
Sbjct: 137 QIFKLDKSSGKKSYILSARELSITWSSDPLYWT 235
>TC8955 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like
protein, partial (12%)
Length = 693
Score = 56.6 bits (135), Expect = 4e-09
Identities = 27/50 (54%), Positives = 35/50 (70%)
Frame = +2
Query: 208 EHNDEWLEIKLGSFYTEKVQVQEVRMCLKEVKGVHLKGGLIVDGIELRPK 257
E D WLE++LG F+ E + +EV M + EVKG KGGL+V GIE+RPK
Sbjct: 47 ERADGWLEMELGEFFCEGGEDREVEMAVCEVKGGDWKGGLVVQGIEIRPK 196
>TC13194 weakly similar to UP|Q94EW1 (Q94EW1) Nictaba, partial (29%)
Length = 471
Score = 50.4 bits (119), Expect = 3e-07
Identities = 21/48 (43%), Positives = 35/48 (72%)
Frame = +1
Query: 210 NDEWLEIKLGSFYTEKVQVQEVRMCLKEVKGVHLKGGLIVDGIELRPK 257
+D WLEI++G F ++ +E++M + E++G K GLI++GIE+RPK
Sbjct: 40 SDGWLEIEMGEFSNSSLEDEELQMSVIEIQGYSSKSGLILEGIEVRPK 183
>AV772932
Length = 481
Score = 47.8 bits (112), Expect = 2e-06
Identities = 24/48 (50%), Positives = 36/48 (75%)
Frame = -3
Query: 210 NDEWLEIKLGSFYTEKVQVQEVRMCLKEVKGVHLKGGLIVDGIELRPK 257
+D W+EI+LG F++ V E++M L+E+ G LKGGL+ +GIE+RPK
Sbjct: 476 DDGWMEIELGEFFSGGGDV-EIKMGLREM-GYQLKGGLVFEGIEVRPK 339
>AV423946
Length = 426
Score = 39.7 bits (91), Expect = 5e-04
Identities = 13/54 (24%), Positives = 31/54 (57%)
Frame = +1
Query: 4 LPEDCFAHILSFTSPLDVCRVSLLSSVVQSMADSDFLWEKFLPHKYQEIISRLV 57
+PE C + + P D+C+++ ++ + +DF+WE LP Y+ + ++++
Sbjct: 241 IPESCISSLFMNLDPPDICKLARVNRAFHRASSADFVWESKLPPSYKFLANKIL 402
>CB828206
Length = 466
Score = 39.3 bits (90), Expect = 7e-04
Identities = 17/48 (35%), Positives = 33/48 (68%)
Frame = +1
Query: 210 NDEWLEIKLGSFYTEKVQVQEVRMCLKEVKGVHLKGGLIVDGIELRPK 257
+D LEI++G F+ ++ +E+++ + EV + K GL ++GIE+RP+
Sbjct: 61 SDG*LEIEMGEFFNSGLEDEELQLSILEVNSRNWKSGLHLEGIEVRPR 204
>TC8893
Length = 509
Score = 31.6 bits (70), Expect = 0.14
Identities = 15/46 (32%), Positives = 23/46 (49%), Gaps = 1/46 (2%)
Frame = +1
Query: 212 EWLEIKLGSFYTEKVQVQ-EVRMCLKEVKGVHLKGGLIVDGIELRP 256
EW +G F E+ V ++ L ++ H KGGL +DG + P
Sbjct: 82 EWAFYHVGDFAIERPNVSTNIKFSLAQIDCTHTKGGLCIDGAVICP 219
>BP048367
Length = 205
Score = 30.8 bits (68), Expect = 0.25
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = -1
Query: 240 GVHLKGGLIVDGIELRPK 257
G LKGGLI++G+E+RPK
Sbjct: 202 GYPLKGGLIIEGLEVRPK 149
>AV771384
Length = 359
Score = 27.7 bits (60), Expect = 2.1
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 3/43 (6%)
Frame = -3
Query: 17 SPLD---VCRVSLLSSVVQSMADSDFLWEKFLPHKYQEIISRL 56
SP D VCR L+ + Q S F W F+PH +E++ +L
Sbjct: 198 SPRDWV*VCR-HLMYLITQGRTASPFAWNNFIPH-VKEVVKKL 76
>BP034385
Length = 481
Score = 27.7 bits (60), Expect = 2.1
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +2
Query: 4 LPEDCFAHILSFTSPLDVCRVSLLSSVVQSMADSDFLWEK 43
+P + F HIL F S D+ S + + + A + LW +
Sbjct: 362 IPPELFHHILKFLSSEDLISCSAVCTFLNYAASDEALWRR 481
>TC13195
Length = 482
Score = 27.7 bits (60), Expect = 2.1
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = +3
Query: 111 TSLYWSWKQVQGSRFAEAAE 130
TS +W WKQ GS A AE
Sbjct: 357 TSTFWKWKQSNGSSEASGAE 416
>AV423739
Length = 465
Score = 27.3 bits (59), Expect = 2.7
Identities = 13/50 (26%), Positives = 26/50 (52%)
Frame = +1
Query: 4 LPEDCFAHILSFTSPLDVCRVSLLSSVVQSMADSDFLWEKFLPHKYQEII 53
LP+ IL P +C+++ + ++ SD LWE+ + K+ ++I
Sbjct: 106 LPDLALECILERLPPSALCQMAGVCRSLRESCVSDHLWERHMKQKWGKVI 255
>AV415704
Length = 355
Score = 26.6 bits (57), Expect = 4.6
Identities = 12/42 (28%), Positives = 19/42 (44%)
Frame = +1
Query: 4 LPEDCFAHILSFTSPLDVCRVSLLSSVVQSMADSDFLWEKFL 45
LP+D I S D+C + S + + SD +WE +
Sbjct: 10 LPQDVTFKITSLLQVRDLCALGCCSRFCRDLCFSDCIWESLV 135
>AW163944
Length = 434
Score = 26.2 bits (56), Expect = 6.1
Identities = 13/39 (33%), Positives = 24/39 (61%)
Frame = -1
Query: 144 NSEMLSPKTMYGAYLKVKIADRAYGLDSLPSEVSIEVGN 182
N++ L+PK++ G L ++ +G +SLP + + VGN
Sbjct: 404 NTKTLTPKSVKGPSLSLESIHHIHGSNSLPPSM-LSVGN 291
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.135 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,092,924
Number of Sequences: 28460
Number of extensions: 72003
Number of successful extensions: 385
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 377
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 381
length of query: 261
length of database: 4,897,600
effective HSP length: 88
effective length of query: 173
effective length of database: 2,393,120
effective search space: 414009760
effective search space used: 414009760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144513.9