BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144513.18 + phase: 0
(107 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15503 similar to UP|O04018 (O04018) F7G19.1 protein, partial (... 94 5e-21
BP038767 31 0.028
TC9450 similar to GB|AAM16172.1|20334832|AY094016 At2g39720/T5I7... 30 0.047
TC15031 weakly similar to UP|Q69107 (Q69107) LAT protein, partia... 29 0.11
TC14473 similar to UP|Q8H1Y2 (Q8H1Y2) 20S proteasome alpha 6 sub... 29 0.14
TC15102 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, ... 28 0.18
TC8380 27 0.40
AV409919 27 0.40
TC17191 homologue to UP|Q9SL38 (Q9SL38) At2g18090 protein, parti... 27 0.40
TC12962 similar to AAQ89629 (AAQ89629) At3g50330, partial (27%) 27 0.40
TC8908 similar to UP|Q8LAN0 (Q8LAN0) ATP-dependent Clp protease ... 27 0.52
TC11199 27 0.68
BP037240 26 0.89
TC17661 weakly similar to UP|Q09085 (Q09085) Hydroxyproline-rich... 26 0.89
BP041113 26 0.89
TC18341 similar to UP|Q25936 (Q25936) Major merozoite surface an... 26 1.2
TC7876 homologue to UP|GBLP_SOYBN (Q39836) Guanine nucleotide-bi... 25 1.5
BI420016 25 1.5
TC19682 similar to UP|O65169 (O65169) Glutaredoxin I, partial (54%) 25 1.5
TC14875 similar to UP|Q9VNX6 (Q9VNX6) CG7421-PA, partial (4%) 25 1.5
>TC15503 similar to UP|O04018 (O04018) F7G19.1 protein, partial (45%)
Length = 590
Score = 93.6 bits (231), Expect = 5e-21
Identities = 60/108 (55%), Positives = 73/108 (67%), Gaps = 13/108 (12%)
Frame = +1
Query: 1 MATCFRVPISMPTSMPASST-----RFTPTR-----LRTLRTVNAISKSKIP---FNPND 47
MAT RVP ++PTS+P SS+ + P+R LR+ +VNA +++KIP NPND
Sbjct: 52 MATYLRVP-AVPTSIPTSSSSSNSQQSLPSRRRCRNLRSFCSVNA-TRAKIPTPHLNPND 225
Query: 48 PFLSKLASVAESSPETLFNPSSSPDNLPFLDIFDSPQLMATPAQVSNS 95
PFLSKLASVA SSPE L N + D P+LDIFDSP LMATPAQV S
Sbjct: 226 PFLSKLASVAASSPEMLLNRPVNSDTPPYLDIFDSPTLMATPAQVERS 369
>BP038767
Length = 561
Score = 31.2 bits (69), Expect = 0.028
Identities = 20/53 (37%), Positives = 25/53 (46%), Gaps = 2/53 (3%)
Frame = +2
Query: 23 TPTRLRTLRTVN--AISKSKIPFNPNDPFLSKLASVAESSPETLFNPSSSPDN 73
TPTR N S S +P P P S +S + +PET NP +S DN
Sbjct: 266 TPTRRSEPAATNNKTPSPSPLPKKPPSPSPSTRSSAKKKTPETPTNPDASLDN 424
>TC9450 similar to GB|AAM16172.1|20334832|AY094016 At2g39720/T5I7.2
{Arabidopsis thaliana;}, partial (22%)
Length = 646
Score = 30.4 bits (67), Expect = 0.047
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +3
Query: 12 PTSMPASSTRFTPTRLRTLRTVNAISK-SKIPFNPNDPFLSKLASVAESSP 61
PT+ PAS R TPT R T+ ++ KIPF P + A+ E++P
Sbjct: 120 PTAKPASLKRSTPTNHRITLTLTVSARYLKIPFLPGG*VSAADAATPEAAP 272
>TC15031 weakly similar to UP|Q69107 (Q69107) LAT protein, partial (27%)
Length = 997
Score = 29.3 bits (64), Expect = 0.11
Identities = 24/90 (26%), Positives = 38/90 (41%), Gaps = 5/90 (5%)
Frame = +3
Query: 12 PTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLA-----SVAESSPETLFN 66
P+S P+SS+ F+P+ +S S NPN +SK + SV+ SS +
Sbjct: 492 PSSPPSSSSLFSPS----------VSSSTFVSNPNPSPISKASTASGPSVSSSSSSSSSG 641
Query: 67 PSSSPDNLPFLDIFDSPQLMATPAQVSNSN 96
PS + P SP P +++
Sbjct: 642 PSPNSSGYPSSAAGTSPPSPIKPISAESTS 731
>TC14473 similar to UP|Q8H1Y2 (Q8H1Y2) 20S proteasome alpha 6 subunit,
partial (86%)
Length = 1302
Score = 28.9 bits (63), Expect = 0.14
Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 5/63 (7%)
Frame = +1
Query: 12 PTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPE-----TLFN 66
PTS +STR TP L T R + + + +P+ P L+ ++S A P T N
Sbjct: 262 PTSSSPASTRLTPNSLLTRRRSSRLMTTSASPSPDSP-LTAVSSPATCDPSVSTTLTPMN 438
Query: 67 PSS 69
P S
Sbjct: 439 PLS 447
>TC15102 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, partial
(68%)
Length = 1097
Score = 28.5 bits (62), Expect = 0.18
Identities = 28/93 (30%), Positives = 37/93 (39%)
Frame = +1
Query: 3 TCFRVPISMPTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPE 62
TC P S PT P SS TP R+ T KSK + P AS A ++P
Sbjct: 265 TCLLAPASPPT--PKSSHSSTPPPSRSSSTT---PKSKNATSLTAPSCPPPASPAATAPA 429
Query: 63 TLFNPSSSPDNLPFLDIFDSPQLMATPAQVSNS 95
P + P N S TP+ +S++
Sbjct: 430 PSKTPPNPPTNSSKPSSCKSSLPNTTPSSLSSA 528
>TC8380
Length = 926
Score = 27.3 bits (59), Expect = 0.40
Identities = 26/83 (31%), Positives = 33/83 (39%), Gaps = 2/83 (2%)
Frame = -2
Query: 25 TRLRTLRTVNAISKSKIPFNPNDPFL--SKLASVAESSPETLFNPSSSPDNLPFLDIFDS 82
TR+R ++ N S P NPNDP L S L P P + IF +
Sbjct: 346 TRMREIKEKNRAS----PLNPNDPLLIKSSLLHCCRIPPPPPPPPVTGSAGSGCTSIFTA 179
Query: 83 PQLMATPAQVSNSNFSFHFLQIE 105
L + SN + F FL IE
Sbjct: 178 ELL-----EESNISICFFFLWIE 125
>AV409919
Length = 423
Score = 27.3 bits (59), Expect = 0.40
Identities = 14/32 (43%), Positives = 17/32 (52%)
Frame = +3
Query: 42 PFNPNDPFLSKLASVAESSPETLFNPSSSPDN 73
P N + P SKL SP F+PSSSP +
Sbjct: 111 PANASKPRTSKLPPPPLPSPTLYFSPSSSPSS 206
>TC17191 homologue to UP|Q9SL38 (Q9SL38) At2g18090 protein, partial (5%)
Length = 598
Score = 27.3 bits (59), Expect = 0.40
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 3 TCFRVPISMPTSMPASSTRFTPTRLRTLR 31
+CFR P+S PTS+P + R +LR
Sbjct: 160 SCFRNPLSRPTSLPLQNDTVGDCRCWSLR 246
>TC12962 similar to AAQ89629 (AAQ89629) At3g50330, partial (27%)
Length = 545
Score = 27.3 bits (59), Expect = 0.40
Identities = 21/86 (24%), Positives = 38/86 (43%), Gaps = 7/86 (8%)
Frame = +2
Query: 23 TPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNPSSSPDNLPFLDIFDS 82
TP + + T+N+ N ND + + SPE+ P+S +N + F
Sbjct: 5 TPNLIS*MNTINSA-------NLNDDKMHTMPMWMHHSPES---PASHGNNNNTVTSFPP 154
Query: 83 PQLMATPAQVSNSN-------FSFHF 101
PQ+ ++ ++N+N F+ HF
Sbjct: 155 PQIFSSATSINNNNPPTPWSTFTHHF 232
>TC8908 similar to UP|Q8LAN0 (Q8LAN0) ATP-dependent Clp protease
proteolytic subunit (ClpP4), partial (35%)
Length = 598
Score = 26.9 bits (58), Expect = 0.52
Identities = 23/78 (29%), Positives = 31/78 (39%), Gaps = 6/78 (7%)
Frame = +1
Query: 17 ASSTRFTPTRLRTL------RTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNPSSS 70
+SS F+P+R+RTL + N S P + P L + S F P S
Sbjct: 79 SSSLPFSPSRIRTLFSKPPTSSFNPKPSSPSPCHRTTPLKCILTASPNHSKNANFEPESL 258
Query: 71 PDNLPFLDIFDSPQLMAT 88
+ F SPQ AT
Sbjct: 259 KPRITFQ--MSSPQTPAT 306
>TC11199
Length = 743
Score = 26.6 bits (57), Expect = 0.68
Identities = 23/83 (27%), Positives = 32/83 (37%)
Frame = +1
Query: 8 PISMPTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNP 67
P S S PAS + T T +L P +P ++ + SSP T+ +P
Sbjct: 160 PSSARPSSPASQSHSTTTSATSLA----------PKSPRSKRSARRTTRTSSSPSTISDP 309
Query: 68 SSSPDNLPFLDIFDSPQLMATPA 90
SS N P L TP+
Sbjct: 310 SSPTSNPS-----SPPSLTPTPS 363
>BP037240
Length = 484
Score = 26.2 bits (56), Expect = 0.89
Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
Frame = +3
Query: 29 TLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETL--FNPSSSPDNLPF 76
TLRT +++ ++ +P P+ P + SSP + F PSSSP PF
Sbjct: 186 TLRT-HSLLQTPLPNLPSPP------PTSTSSPSLILPFKPSSSPPTFPF 314
>TC17661 weakly similar to UP|Q09085 (Q09085) Hydroxyproline-rich
glycoprotein (HRGP) (Fragment), partial (8%)
Length = 417
Score = 26.2 bits (56), Expect = 0.89
Identities = 22/84 (26%), Positives = 32/84 (37%)
Frame = +3
Query: 13 TSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNPSSSPD 72
T+MP SS FT + T T ++ PF+ P + S++ P + PD
Sbjct: 54 TTMPFSSLTFTLSNSSTTPTWTTANQPTPPFHTLQPTPPQTFSISLHKPHLFRIHCTKPD 233
Query: 73 NLPFLDIFDSPQLMATPAQVSNSN 96
L L D A N+N
Sbjct: 234 PLAPLPKHDPTFQPAIGGNDDNNN 305
>BP041113
Length = 503
Score = 26.2 bits (56), Expect = 0.89
Identities = 25/79 (31%), Positives = 34/79 (42%), Gaps = 11/79 (13%)
Frame = -2
Query: 10 SMPTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETL----F 65
S P S P+SST+ PT + + + +SKS P D S+P TL F
Sbjct: 211 STPHSNPSSSTQANPTPSQLMPSAMKVSKS----TPRD-----------STPHTLKYNTF 77
Query: 66 NP-------SSSPDNLPFL 77
+P S P+ PFL
Sbjct: 76 SP*TD*KACSICPEEYPFL 20
>TC18341 similar to UP|Q25936 (Q25936) Major merozoite surface antigen
(Fragment), partial (5%)
Length = 497
Score = 25.8 bits (55), Expect = 1.2
Identities = 17/62 (27%), Positives = 27/62 (43%)
Frame = +2
Query: 10 SMPTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNPSS 69
S P +S +RF P+ + S K +NP+ P S V E + + + NP +
Sbjct: 80 SSPLGFNSSPSRFKPSSPLS-------SPFKGRYNPDRPLSSVTEEVPEKAKDGVMNPPT 238
Query: 70 SP 71
P
Sbjct: 239 PP 244
>TC7876 homologue to UP|GBLP_SOYBN (Q39836) Guanine nucleotide-binding
protein beta subunit-like protein, complete
Length = 1379
Score = 25.4 bits (54), Expect = 1.5
Identities = 25/81 (30%), Positives = 34/81 (41%), Gaps = 23/81 (28%)
Frame = +1
Query: 12 PTSMPASSTRFTPTRLRTLR-------------TVNAISKSKIPFNP----------NDP 48
PT PA+++ TPT RTL TV++ S + +P P + P
Sbjct: 223 PTVSPAAASPVTPTLFRTLSSHLMASSPSPAHGTVSSASGTLLPAPPPAASSVTPRTSSP 402
Query: 49 FLSKLASVAESSPETLFNPSS 69
LS L +V S P PSS
Sbjct: 403 SLSPLTTV-RSCPPPATAPSS 462
>BI420016
Length = 559
Score = 25.4 bits (54), Expect = 1.5
Identities = 19/63 (30%), Positives = 31/63 (49%)
Frame = +2
Query: 8 PISMPTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNP 67
P+S P+S P+ T +P + + S P +P P S + SSP +LF+P
Sbjct: 305 PLS-PSSPPSPPTPPSPPPSPAPNPTPSATGSTTPSSPLTP-----TSASLSSPSSLFSP 466
Query: 68 SSS 70
+S+
Sbjct: 467 AST 475
>TC19682 similar to UP|O65169 (O65169) Glutaredoxin I, partial (54%)
Length = 551
Score = 25.4 bits (54), Expect = 1.5
Identities = 9/16 (56%), Positives = 14/16 (87%)
Frame = -1
Query: 22 FTPTRLRTLRTVNAIS 37
F+PTR+R + TVNA++
Sbjct: 116 FSPTRIRKITTVNAVA 69
>TC14875 similar to UP|Q9VNX6 (Q9VNX6) CG7421-PA, partial (4%)
Length = 1240
Score = 25.4 bits (54), Expect = 1.5
Identities = 20/69 (28%), Positives = 28/69 (39%), Gaps = 1/69 (1%)
Frame = +1
Query: 8 PISMPTSMPASST-RFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFN 66
P++ PT+ P+SST P+ L T + S S SSP T +
Sbjct: 202 PLTPPTTSPSSSTSTAAPSASAPLTTTPTTATST---------TSPRRQTPSSSPSTTAS 354
Query: 67 PSSSPDNLP 75
P S+P P
Sbjct: 355 PRSTPSPSP 381
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.129 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,874,170
Number of Sequences: 28460
Number of extensions: 24353
Number of successful extensions: 237
Number of sequences better than 10.0: 100
Number of HSP's better than 10.0 without gapping: 236
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 236
length of query: 107
length of database: 4,897,600
effective HSP length: 83
effective length of query: 24
effective length of database: 2,535,420
effective search space: 60850080
effective search space used: 60850080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)
Medicago: description of AC144513.18