BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144502.7 - phase: 2
(168 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10393 304 6e-84
TC12218 211 4e-56
TC18373 92 3e-20
BP060308 85 7e-18
TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding pro... 45 5e-06
TC10187 similar to GB|AAM91395.1|22137100|AY133565 At4g14410/dl3... 39 4e-04
TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4... 35 0.009
CB827320 34 0.015
TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium t... 33 0.025
TC19923 30 0.21
TC13829 similar to UP|P93321 (P93321) Cdc2MsD protein, partial (... 30 0.28
TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPas... 28 1.1
TC18869 similar to UP|Q9SIR1 (Q9SIR1) At2g25320 protein, partial... 27 1.4
TC8264 similar to GB|AAK20861.1|13377784|AF333974 fasciclin-like... 27 2.4
BP030979 27 2.4
AV424690 27 2.4
BI418945 26 3.1
TC17658 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp prot... 26 3.1
BP034914 26 4.0
TC11985 similar to GB|AAF40452.1|7211981|F13M7 ESTs gb|N65605, g... 25 6.9
>TC10393
Length = 1063
Score = 304 bits (778), Expect = 6e-84
Identities = 151/168 (89%), Positives = 158/168 (93%)
Frame = +1
Query: 1 VRSESCAATSSKACREKLRRDRLNDKFIELGSILEPGRPAKTDKAAILIDAVRMVTQLRG 60
VRSESC+ATSSKACREKLRRD+LNDKF+ELGSILEPGRP KTDKAAILIDAVRMVTQLRG
Sbjct: 400 VRSESCSATSSKACREKLRRDKLNDKFVELGSILEPGRPPKTDKAAILIDAVRMVTQLRG 579
Query: 61 EAQKLKDANSGLQEKIKELKVEKNELRDEKQRLKAEKEKLEQQLKSMNAPPSFLPTPTAL 120
EAQK+KD N GLQEKIKELK EKNELRDEKQRLK EKE+LEQQLKSMNA PSF+P P AL
Sbjct: 580 EAQKMKDTNMGLQEKIKELKTEKNELRDEKQRLKTEKERLEQQLKSMNAQPSFMPPPQAL 759
Query: 121 PAAFAAQGQAHGNKLVPFISYPGVAMWQFMPPAAVDTSQDHVLRPPVA 168
PAAFAAQGQAHGNKLVPFISYPGVAMWQF+PPAA DTSQDH LRPPVA
Sbjct: 760 PAAFAAQGQAHGNKLVPFISYPGVAMWQFLPPAARDTSQDHELRPPVA 903
>TC12218
Length = 737
Score = 211 bits (538), Expect = 4e-56
Identities = 108/169 (63%), Positives = 136/169 (79%), Gaps = 1/169 (0%)
Frame = +2
Query: 1 VRSESCAATSSKACREKLRRDRLNDKFIELGSILEPGRPAKTDKAAILIDAVRMVTQLRG 60
+R+ES + + SKACREKLRRD+LN++F+EL SILEPGR KTDKAAI+ DAVR+VTQLR
Sbjct: 248 LRTES-SVSGSKACREKLRRDKLNERFLELSSILEPGRQPKTDKAAIISDAVRVVTQLRN 424
Query: 61 EAQKLKDANSGLQEKIKELKVEKNELRDEKQRLKAEKEKLEQQLKSMNAPPSFLPTPTAL 120
EA+KLK+ N+ LQEKIKELK EKNE+RDEK +LK +KEKLE+++K N P FLP A
Sbjct: 425 EAEKLKEMNNDLQEKIKELKAEKNEIRDEKNKLKLDKEKLEKKVKLRNVQPGFLPHADA- 601
Query: 121 PAAFAAQGQ-AHGNKLVPFISYPGVAMWQFMPPAAVDTSQDHVLRPPVA 168
A +G+ A +KL+P+I YPG+AMWQFMP A +DTS+DH+LRPPVA
Sbjct: 602 ----AVKGKGAASHKLIPYIGYPGIAMWQFMPSAVLDTSRDHLLRPPVA 736
>TC18373
Length = 437
Score = 92.4 bits (228), Expect = 3e-20
Identities = 42/71 (59%), Positives = 57/71 (80%)
Frame = +1
Query: 2 RSESCAATSSKACREKLRRDRLNDKFIELGSILEPGRPAKTDKAAILIDAVRMVTQLRGE 61
R++SC +KACREK RR++LN++F EL S+LEPGRP KTDK AIL DA+R+++QL+ E
Sbjct: 220 RTDSCCKAGTKACREKQRREKLNERFCELSSVLEPGRPVKTDKPAILDDAIRVLSQLKTE 399
Query: 62 AQKLKDANSGL 72
AQ+LK+ N L
Sbjct: 400 AQELKETNEKL 432
>BP060308
Length = 354
Score = 84.7 bits (208), Expect = 7e-18
Identities = 39/58 (67%), Positives = 45/58 (77%)
Frame = -3
Query: 111 PSFLPTPTALPAAFAAQGQAHGNKLVPFISYPGVAMWQFMPPAAVDTSQDHVLRPPVA 168
PSFLP + LP AF A G++L+P I YPGV+MWQF+P AAVDTSQDHVLRPPVA
Sbjct: 352 PSFLPHHSTLPTAFPAPRHVVGSQLMPIIGYPGVSMWQFLPSAAVDTSQDHVLRPPVA 179
>TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding protein PG2
(Fragment), partial (30%)
Length = 705
Score = 45.4 bits (106), Expect = 5e-06
Identities = 28/96 (29%), Positives = 49/96 (50%)
Frame = +2
Query: 16 EKLRRDRLNDKFIELGSILEPGRPAKTDKAAILIDAVRMVTQLRGEAQKLKDANSGLQEK 75
E+ RR++LN +F L +++ +K DKA++L DA+ +T+L+ + Q + +GLQ++
Sbjct: 203 ERQRREKLNQRFYALRAVVP--NVSKMDKASLLGDAISYITELKTKLQSSESDKTGLQKQ 376
Query: 76 IKELKVEKNELRDEKQRLKAEKEKLEQQLKSMNAPP 111
+K E EK +Q S PP
Sbjct: 377 F--------------DAMKKELEKTSEQSSSPTPPP 442
>TC10187 similar to GB|AAM91395.1|22137100|AY133565 At4g14410/dl3245w
{Arabidopsis thaliana;}, partial (10%)
Length = 444
Score = 39.3 bits (90), Expect = 4e-04
Identities = 15/25 (60%), Positives = 19/25 (76%)
Frame = +2
Query: 144 VAMWQFMPPAAVDTSQDHVLRPPVA 168
+ MWQ++P +A DTS DH LRPP A
Sbjct: 8 IPMWQYLPSSARDTSHDHELRPPAA 82
>TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4240w
{Arabidopsis thaliana;}, partial (29%)
Length = 604
Score = 34.7 bits (78), Expect = 0.009
Identities = 23/69 (33%), Positives = 38/69 (54%)
Frame = +3
Query: 16 EKLRRDRLNDKFIELGSILEPGRPAKTDKAAILIDAVRMVTQLRGEAQKLKDANSGLQEK 75
E+ RR++LN +F L +++ +K DKA++L DA+ +T LQ+K
Sbjct: 426 ERQRREKLNQRFYALRAVVP--NISKMDKASLLGDAITHITD--------------LQKK 557
Query: 76 IKELKVEKN 84
IK L+ EK+
Sbjct: 558 IKLLETEKD 584
>CB827320
Length = 522
Score = 33.9 bits (76), Expect = 0.015
Identities = 26/99 (26%), Positives = 47/99 (47%), Gaps = 1/99 (1%)
Frame = +1
Query: 7 AATSSKACREKLRRDRLNDKFIELGSILEPGRPAKTDKAAILIDAVRMVTQLRGEAQKLK 66
+A S E+ RR +LN++ L S++ +K DKA+I+ DA+ + L + + ++
Sbjct: 217 SAASKNIVSERNRRKKLNERLFALRSVVP--NISKMDKASIIKDAIEYIEHLHEQEKIIQ 390
Query: 67 DANSGLQEKI-KELKVEKNELRDEKQRLKAEKEKLEQQL 104
L+ I K + + E L K+K +QL
Sbjct: 391 AEIMELESGIPKNMNNPSYDFDQELPVLLRSKKKRTEQL 507
>TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium
transporter, partial (34%)
Length = 1007
Score = 33.1 bits (74), Expect = 0.025
Identities = 19/54 (35%), Positives = 34/54 (62%)
Frame = +1
Query: 16 EKLRRDRLNDKFIELGSILEPGRPAKTDKAAILIDAVRMVTQLRGEAQKLKDAN 69
E+ RR++L+ I L +++ PG K DKA++L DA++ V L+ + L++ N
Sbjct: 43 ERRRREKLSQSLIALAALI-PGLK-KMDKASVLGDAIKYVKVLKERLRLLEEQN 198
>TC19923
Length = 700
Score = 30.0 bits (66), Expect = 0.21
Identities = 28/111 (25%), Positives = 47/111 (42%), Gaps = 23/111 (20%)
Frame = +1
Query: 81 VEKNELRDEKQRLKAEKEKLEQQLKS--------MNAPP----------SFLPTPTALPA 122
++KNEL++E L+ + EKL+ ++++ +NAPP +F +P
Sbjct: 19 MKKNELKEENCSLETQIEKLQGEIQARVAQSKPDLNAPPHLELEPPEQTNFPGQSIQVPT 198
Query: 123 AFAAQGQAHGNKLVPF-----ISYPGVAMWQFMPPAAVDTSQDHVLRPPVA 168
Q +VPF ++P + + MP A SQ H P A
Sbjct: 199 MEPTLPQGPAVLVVPFRPDLQTAFPVPNISELMPKPAPVVSQPHARYPTPA 351
>TC13829 similar to UP|P93321 (P93321) Cdc2MsD protein, partial (43%)
Length = 515
Score = 29.6 bits (65), Expect = 0.28
Identities = 14/58 (24%), Positives = 28/58 (48%)
Frame = +3
Query: 64 KLKDANSGLQEKIKELKVEKNELRDEKQRLKAEKEKLEQQLKSMNAPPSFLPTPTALP 121
KL++ G + I++ + E+ +R + + ++ + + PP LP P ALP
Sbjct: 87 KLRETGEGRRRHIRQSLQSQREIHRPSRRSQENPSRDGRRRRPSDRPPRSLPPPDALP 260
>TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPase subunit
RPT4a, partial (49%)
Length = 648
Score = 27.7 bits (60), Expect = 1.1
Identities = 25/71 (35%), Positives = 32/71 (44%)
Frame = +2
Query: 39 PAKTDKAAILIDAVRMVTQLRGEAQKLKDANSGLQEKIKELKVEKNELRDEKQRLKAEKE 98
P TD A DAVR T + +KL LQ K EL+ +R+ Q K E
Sbjct: 38 PTMTDAAE---DAVRRRTAVADYRKKL------LQHK--ELESRVRSVRENLQASKKEYN 184
Query: 99 KLEQQLKSMNA 109
K E LKS+ +
Sbjct: 185 KTEDDLKSLQS 217
>TC18869 similar to UP|Q9SIR1 (Q9SIR1) At2g25320 protein, partial (8%)
Length = 381
Score = 27.3 bits (59), Expect = 1.4
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = +1
Query: 62 AQKLKDANSGLQEKIKELKVEKNELRDEKQRLKAEKEKLEQQL 104
+++ K+ +QE +L+ ++E DEK +L AEK+ L+ +L
Sbjct: 4 SREKKELTEQVQEIESQLEWLRSERDDEKVKLSAEKKVLQDRL 132
>TC8264 similar to GB|AAK20861.1|13377784|AF333974 fasciclin-like
arabinogalactan-protein 9 {Arabidopsis thaliana;},
partial (38%)
Length = 942
Score = 26.6 bits (57), Expect = 2.4
Identities = 20/65 (30%), Positives = 27/65 (40%)
Frame = -3
Query: 101 EQQLKSMNAPPSFLPTPTALPAAFAAQGQAHGNKLVPFISYPGVAMWQFMPPAAVDTSQD 160
E+ MN P P PT LPA + A G+ PF GV+ + D ++D
Sbjct: 760 ERAPMQMNPSPRTNPKPTFLPATPLSFWLAAGDGAPPFFPELGVSSEGVVSDDLGDGAED 581
Query: 161 HVLRP 165
L P
Sbjct: 580 GDLAP 566
>BP030979
Length = 510
Score = 26.6 bits (57), Expect = 2.4
Identities = 26/94 (27%), Positives = 38/94 (39%), Gaps = 6/94 (6%)
Frame = +3
Query: 30 LGSILEPGRPAKTDKAAILIDA-VRMVTQLRGEAQKLKD-ANSGLQEKIKELKVEKN--- 84
+ ++L P R AK AA L + + + GE L A EK + + K
Sbjct: 9 IAAVLRPQREAKKRAAAALCELRPKKKRVVXGELTNLSSPAEKPTSEKRRRVGKSKAPPP 188
Query: 85 -ELRDEKQRLKAEKEKLEQQLKSMNAPPSFLPTP 117
E RD+ Q + + L+SM PS P P
Sbjct: 189 PEKRDDPQMCLPYVSDIYEYLRSMEVDPSKRPLP 290
>AV424690
Length = 416
Score = 26.6 bits (57), Expect = 2.4
Identities = 19/78 (24%), Positives = 31/78 (39%)
Frame = +2
Query: 75 KIKELKVEKNELRDEKQRLKAEKEKLEQQLKSMNAPPSFLPTPTALPAAFAAQGQAHGNK 134
KIK + + + ++ ++ APP+ P+PT Q Q +
Sbjct: 47 KIKNFQPHQELETCSNEHVRPKRSSFHSSKPIFPAPPTPTPSPTHF---IRPQFQP*SS- 214
Query: 135 LVPFISYPGVAMWQFMPP 152
PFIS+P +W F P
Sbjct: 215 --PFISFPQCWLWHFPSP 262
>BI418945
Length = 523
Score = 26.2 bits (56), Expect = 3.1
Identities = 19/71 (26%), Positives = 30/71 (41%)
Frame = +2
Query: 58 LRGEAQKLKDANSGLQEKIKELKVEKNELRDEKQRLKAEKEKLEQQLKSMNAPPSFLPTP 117
LRG K KD L ++K+ + E+N+L L ++L+ L S + S P
Sbjct: 125 LRGSVMKEKDEELALFLEMKKREKERNDL------LLLNSDELDSPLGSNPSTSSIFNIP 286
Query: 118 TALPAAFAAQG 128
+ P G
Sbjct: 287 ASAPIPVRKNG 319
>TC17658 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp protease
ATP-binding subunit clpA homolog, chloroplast precursor
, partial (33%)
Length = 908
Score = 26.2 bits (56), Expect = 3.1
Identities = 28/76 (36%), Positives = 42/76 (54%), Gaps = 10/76 (13%)
Frame = +1
Query: 43 DKAAILIDA----VRMV-TQLRGEAQKL-KDANSGLQEK---IKELKVEK-NELRDEKQR 92
DKA LID VR+ QL EA++L K+ ++EK ++ EK ELRD++
Sbjct: 76 DKAIDLIDEAGSRVRLQHAQLPEEARELDKEVRLIIKEKEESVRNQDFEKAGELRDKEMD 255
Query: 93 LKAEKEKLEQQLKSMN 108
LKA+ L ++ K M+
Sbjct: 256 LKAQISALVEKGKEMS 303
>BP034914
Length = 544
Score = 25.8 bits (55), Expect = 4.0
Identities = 12/28 (42%), Positives = 15/28 (52%)
Frame = +1
Query: 110 PPSFLPTPTALPAAFAAQGQAHGNKLVP 137
PP+ + TPTALP A + A G P
Sbjct: 100 PPTPIRTPTALPGAAVRRSAAEGAMEAP 183
>TC11985 similar to GB|AAF40452.1|7211981|F13M7 ESTs gb|N65605, gb|N38087,
gb|T20485, gb|T13726, gb|N38339, gb|F15440 and gb|N97201
come from this gene. {Arabidopsis thaliana;}, partial
(88%)
Length = 1334
Score = 25.0 bits (53), Expect = 6.9
Identities = 10/23 (43%), Positives = 17/23 (73%)
Frame = +2
Query: 77 KELKVEKNELRDEKQRLKAEKEK 99
+ELK ++ ELR+ ++ K E+EK
Sbjct: 458 EELKAKQQELRERARKKKEEEEK 526
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.130 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,411,423
Number of Sequences: 28460
Number of extensions: 28613
Number of successful extensions: 178
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of query: 168
length of database: 4,897,600
effective HSP length: 84
effective length of query: 84
effective length of database: 2,506,960
effective search space: 210584640
effective search space used: 210584640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144502.7