BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144481.8 + phase: 0 /pseudo
(119 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17684 70 9e-14
TC10426 similar to PIR|S22384|S22384 dihydrolipoamide dehydrogen... 33 0.013
TC10886 similar to UP|Q8GSM8 (Q8GSM8) Squalene monooxygenase 1 ... 32 0.037
TC14964 weakly similar to UP|SOX_ARATH (Q9SJA7) Potential sarcos... 31 0.048
TC14012 similar to UP|Q8GSM9 (Q8GSM9) Squalene monooxygenase 2 ... 30 0.11
TC13616 homologue to UP|Q93YZ7 (Q93YZ7) At2g31810/F20M17.15, par... 29 0.24
BF177814 28 0.53
TC17079 similar to UP|Q84UV7 (Q84UV7) SGT1, partial (38%) 27 1.2
TC9182 similar to AAR24677 (AAR24677) At3g62040, partial (86%) 27 1.2
TC18172 similar to UP|O81815 (O81815) Monooxygenase, partial (15%) 26 1.5
AV408622 25 2.6
CB827863 25 2.6
TC11728 25 2.6
TC10861 similar to GB|AAM65350.1|21655277|AY103298 At2g20587/At2... 25 4.5
TC18881 similar to UP|Q9FZ15 (Q9FZ15) Tuber-specific and sucrose... 24 5.9
AI967443 24 5.9
TC17189 homologue to UP|MAPK_PEA (Q06060) Mitogen-activated prot... 24 7.7
>TC17684
Length = 721
Score = 70.1 bits (170), Expect = 9e-14
Identities = 49/140 (35%), Positives = 68/140 (48%), Gaps = 28/140 (20%)
Frame = +1
Query: 2 QYDENGENQAWEAIGY-------------------HVDNDESTSNIQKERPLEKGIVEAM 42
Q DE+ +N +W+AIGY +N E Q PL KG+V
Sbjct: 178 QLDESEDNCSWKAIGYCGPDPEFKAQLKNHFLQGGQENNKEEDDAEQVIGPLYKGLVHLN 357
Query: 43 NEDDLSLAQSLSNKGLEIDI-----DANSNI----LKVKCDVVIVGSGCGGGVAAAVLAS 93
N D++ A +L G + + A+ NI L ++CD V+VGSG GGG+ A +LA
Sbjct: 358 NPRDIT-ADALRQFGFPVSVIRRKPKASPNISSPSLVIQCDAVVVGSGSGGGLIAGILAK 534
Query: 94 SGLKVLVLEKGNYFTPRDFS 113
+G KVLV EKG Y + S
Sbjct: 535 AGYKVLVKEKGGYTARNNLS 594
>TC10426 similar to PIR|S22384|S22384 dihydrolipoamide dehydrogenase
precursor - garden pea {Pisum sativum;}
, partial (31%)
Length = 512
Score = 33.1 bits (74), Expect = 0.013
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = +3
Query: 52 SLSNKGLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
S++++G D N DVV++G G GG VAA A GLK +EK
Sbjct: 114 SVASRGFASGSDQN--------DVVVIGGGPGGYVAAIKAAQLGLKTTCIEK 245
>TC10886 similar to UP|Q8GSM8 (Q8GSM8) Squalene monooxygenase 1 , partial
(27%)
Length = 1135
Score = 31.6 bits (70), Expect = 0.037
Identities = 16/39 (41%), Positives = 23/39 (58%)
Frame = +1
Query: 65 NSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
N+NI DV++VG+G G A L G +VLV+E+
Sbjct: 763 NANI-----DVIVVGAGVAGAALAHTLGKDGRQVLVIER 864
>TC14964 weakly similar to UP|SOX_ARATH (Q9SJA7) Potential sarcosine oxidase
, partial (33%)
Length = 612
Score = 31.2 bits (69), Expect = 0.048
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +3
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFS 113
DV+I+G+G G A A GLK L+LE+ ++ R S
Sbjct: 42 DVIIIGAGVIGSATAYQAAKRGLKTLLLEQFDFLHHRGSS 161
>TC14012 similar to UP|Q8GSM9 (Q8GSM9) Squalene monooxygenase 2 , partial
(13%)
Length = 480
Score = 30.0 bits (66), Expect = 0.11
Identities = 15/41 (36%), Positives = 21/41 (50%)
Frame = +2
Query: 63 DANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
+ S L DV+IVG+G G A L G +V V+E+
Sbjct: 278 ECGSEKLDGDADVIIVGAGVAGSALAHTLGKDGRRVHVIER 400
>TC13616 homologue to UP|Q93YZ7 (Q93YZ7) At2g31810/F20M17.15, partial (9%)
Length = 440
Score = 28.9 bits (63), Expect = 0.24
Identities = 13/30 (43%), Positives = 18/30 (59%)
Frame = -3
Query: 78 VGSGCGGGVAAAVLASSGLKVLVLEKGNYF 107
V GCGGG V+A SG +V ++G +F
Sbjct: 267 VSGGCGGGEGEFVVAESGGRVRY*DEGRWF 178
>BF177814
Length = 453
Score = 27.7 bits (60), Expect = 0.53
Identities = 24/64 (37%), Positives = 32/64 (49%), Gaps = 11/64 (17%)
Frame = +3
Query: 43 NEDDLSLAQSLSNKGLEIDIDANSNIL---KVKCDVVIVGSG--------CGGGVAAAVL 91
+EDDL LS+ G + D + S +L K K ++ SG CGGGVA AV
Sbjct: 9 HEDDLK--SILSSVGADADDEKLSFLLSEVKGKDLAEVIASGREKLSSVPCGGGVAVAVS 182
Query: 92 ASSG 95
A+ G
Sbjct: 183AAPG 194
>TC17079 similar to UP|Q84UV7 (Q84UV7) SGT1, partial (38%)
Length = 676
Score = 26.6 bits (57), Expect = 1.2
Identities = 17/44 (38%), Positives = 26/44 (58%), Gaps = 1/44 (2%)
Frame = +1
Query: 23 ESTSNIQKERPLEKGIVEAMNEDDLSLAQSLSNKGLEID-IDAN 65
E+T+ + + LEK EA +DD SLA L + + +D I+AN
Sbjct: 82 ETTTTV---KDLEKKAKEAFIDDDFSLAVDLYTEAINLDPINAN 204
>TC9182 similar to AAR24677 (AAR24677) At3g62040, partial (86%)
Length = 1260
Score = 26.6 bits (57), Expect = 1.2
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 3 YDENGENQAW-EAIGYHVDNDESTSNIQKERPLEK 36
Y E+G A +A+GY DNDE + + P EK
Sbjct: 417 YIEHGTTMAGLKALGYEFDNDEFHAYVHGRLPYEK 521
>TC18172 similar to UP|O81815 (O81815) Monooxygenase, partial (15%)
Length = 597
Score = 26.2 bits (56), Expect = 1.5
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = +2
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEKGN 105
++VIVG G G A L +K LVLE+ +
Sbjct: 89 EIVIVGGGICGLATALALHRKSIKSLVLERSD 184
>AV408622
Length = 415
Score = 25.4 bits (54), Expect = 2.6
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -1
Query: 62 IDANSNILKVKCDVVIVGSGCGGG 85
+ AN++ LK V++G+G GGG
Sbjct: 193 VGANASSLKTVAMAVVIGNGDGGG 122
>CB827863
Length = 571
Score = 25.4 bits (54), Expect = 2.6
Identities = 24/76 (31%), Positives = 34/76 (44%)
Frame = +3
Query: 19 VDNDESTSNIQKERPLEKGIVEAMNEDDLSLAQSLSNKGLEIDIDANSNILKVKCDVVIV 78
VD +ES N+ K ++E L Q L N G E+ + L VK D +++
Sbjct: 66 VDPNESVENV-------KALLEVETSVPLQKQQLLFN-GKEVRNPEKLSALGVKDDDLLM 221
Query: 79 GSGCGGGVAAAVLASS 94
GGG AAA S+
Sbjct: 222 MVSGGGGGAAASSGSA 269
>TC11728
Length = 832
Score = 25.4 bits (54), Expect = 2.6
Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 4/64 (6%)
Frame = +2
Query: 2 QYDENGENQAWEAIGYHVDNDESTSNIQKERPLEKGIVEAMNEDDLSLA----QSLSNKG 57
QY + +A A YH DES + K R + G+V E LS+ + KG
Sbjct: 71 QYVKASGKEAIVAGFYHEGYDESLLMLMKRRGVHSGLVVKGEEGALSMTTRSRSGNTTKG 250
Query: 58 LEID 61
L ++
Sbjct: 251 LPVN 262
>TC10861 similar to GB|AAM65350.1|21655277|AY103298 At2g20587/At2g20587
{Arabidopsis thaliana;}, partial (35%)
Length = 533
Score = 24.6 bits (52), Expect = 4.5
Identities = 15/61 (24%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = -3
Query: 24 STSNIQKERPLEKGIVEAMNEDDLSL---AQSLSNKGLEIDIDANSNILKVKCDVVIVGS 80
ST N++ + E+G++EAM D +++ N L + D ++ V+ + ++G
Sbjct: 213 STGNLRVDWDCERGLLEAMRNSDERADLPSEATRNADLAAEHDREIDLTAVEA-IFVIGV 37
Query: 81 G 81
G
Sbjct: 36 G 34
>TC18881 similar to UP|Q9FZ15 (Q9FZ15) Tuber-specific and sucrose-responsive
element binding factor, partial (28%)
Length = 346
Score = 24.3 bits (51), Expect = 5.9
Identities = 10/34 (29%), Positives = 16/34 (46%)
Frame = +3
Query: 2 QYDENGENQAWEAIGYHVDNDESTSNIQKERPLE 35
Q+ G Q WE +G+H + + + E LE
Sbjct: 237 QHHHQGACQVWEQVGHHSSHPQRPHR*RCEESLE 338
>AI967443
Length = 435
Score = 24.3 bits (51), Expect = 5.9
Identities = 17/65 (26%), Positives = 28/65 (42%), Gaps = 11/65 (16%)
Frame = +2
Query: 3 YDENGENQAWEAIGYHVDNDES---------TSNIQKERPLEKGI--VEAMNEDDLSLAQ 51
+DE+ + Q+W I +V N S N Q E LE+ ++ + E+ L +
Sbjct: 95 FDEHXKEQSWRNIDLNVPNQHSRDLSSDSNDNHNDQLEDELERSTQQLDQLKEEVCQLME 274
Query: 52 SLSNK 56
L K
Sbjct: 275 GLEEK 289
>TC17189 homologue to UP|MAPK_PEA (Q06060) Mitogen-activated protein kinase
homolog D5 , partial (30%)
Length = 513
Score = 23.9 bits (50), Expect = 7.7
Identities = 9/16 (56%), Positives = 12/16 (74%)
Frame = -1
Query: 79 GSGCGGGVAAAVLASS 94
GSGCGGG A+ + S+
Sbjct: 195 GSGCGGGAASDMSVSA 148
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.134 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,726,927
Number of Sequences: 28460
Number of extensions: 18655
Number of successful extensions: 111
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 110
length of query: 119
length of database: 4,897,600
effective HSP length: 79
effective length of query: 40
effective length of database: 2,649,260
effective search space: 105970400
effective search space used: 105970400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 49 (23.5 bits)
Medicago: description of AC144481.8