BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144477.10 - phase: 0 /pseudo
(200 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14593 similar to UP|Q8H1U3 (Q8H1U3) CDC26, partial (31%) 356 2e-99
AU251635 28 1.4
BP062454 28 1.4
TC10531 similar to UP|Q96UV7 (Q96UV7) Endo-1,4-beta-xylanase , ... 27 1.8
TC19366 27 1.8
TC14083 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor... 27 3.1
BE122585 27 3.1
TC20215 similar to AAS10104 (AAS10104) MYB transcription factor,... 26 5.3
AV767982 25 7.0
TC15958 similar to GB|AAP37838.1|30725632|BT008479 At4g24220 {Ar... 25 9.1
>TC14593 similar to UP|Q8H1U3 (Q8H1U3) CDC26, partial (31%)
Length = 1112
Score = 356 bits (913), Expect = 2e-99
Identities = 172/200 (86%), Positives = 189/200 (94%)
Frame = +2
Query: 1 MEVEVKLRLPNADSYHRVTTLLSPFHVITHRQHNLFFDGAGSELSSRRAILRLRFYGDDE 60
MEVEVKLRLPNA+++ +VTTLL+PFHV THRQHNLFFDGA +ELSS+RA+LRLRFY DD
Sbjct: 251 MEVEVKLRLPNAEAHRQVTTLLAPFHVTTHRQHNLFFDGAAAELSSKRAVLRLRFYNDDS 430
Query: 61 RCVVSLKAKAVLVDGVSRVEEDEEDLDPKIGRDCVDEPGKLGLVESRIMGRVKEEFGVVG 120
RCVVSLKAKAVLVDGVSRVEEDEEDLDPK+GRDCV EPGKLG VESRI+GRVKEEFGV G
Sbjct: 431 RCVVSLKAKAVLVDGVSRVEEDEEDLDPKVGRDCVAEPGKLGSVESRILGRVKEEFGVAG 610
Query: 121 ENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKEN 180
ENGFVGLGGF+NVRNVY+WK LKLEVDET FDFGTL+EIECES+DPEEAKR+LE+ LKEN
Sbjct: 611 ENGFVGLGGFRNVRNVYEWKDLKLEVDETMFDFGTLYEIECESADPEEAKRLLEDLLKEN 790
Query: 181 GIDYSYSVASKFAIFRAGKL 200
GIDYSYSVASKFAIFR+GKL
Sbjct: 791 GIDYSYSVASKFAIFRSGKL 850
>AU251635
Length = 383
Score = 27.7 bits (60), Expect = 1.4
Identities = 20/91 (21%), Positives = 43/91 (46%), Gaps = 3/91 (3%)
Frame = +3
Query: 110 GRVKEEFGVVGENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEA 169
G + FG+ G VGL + + + + +++D F+ F + E +P+E
Sbjct: 15 GSIVAVFGL----GTVGLAVAEGAKAAGASRIIGIDIDSNKFERAKNFGVT-EFINPKEH 179
Query: 170 KRILEEF---LKENGIDYSYSVASKFAIFRA 197
++ +++ L + G+DYS+ ++ RA
Sbjct: 180 EKPIQQVIVDLTDGGVDYSFECIGNVSVMRA 272
>BP062454
Length = 568
Score = 27.7 bits (60), Expect = 1.4
Identities = 15/39 (38%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = +2
Query: 12 ADSYHRVTTLLSPFHVITHRQHNLF--FDGAGSELSSRR 48
A+ +H+ TTL+ P + +H+QH LF F S L +R
Sbjct: 92 ANLHHKQTTLVPPNSLHSHQQHLLFHSFPFCSSPLDEQR 208
>TC10531 similar to UP|Q96UV7 (Q96UV7) Endo-1,4-beta-xylanase , partial
(6%)
Length = 387
Score = 27.3 bits (59), Expect = 1.8
Identities = 20/54 (37%), Positives = 26/54 (48%)
Frame = -3
Query: 117 GVVGENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAK 170
GVV E G +GLGG + V G L+ D H D+ E + DPE A+
Sbjct: 307 GVVVELGELGLGGVEGV-------GTGLDEDPYHEDYEAG*EEDGGEEDPEGAE 167
>TC19366
Length = 453
Score = 27.3 bits (59), Expect = 1.8
Identities = 14/30 (46%), Positives = 19/30 (62%)
Frame = -3
Query: 6 KLRLPNADSYHRVTTLLSPFHVITHRQHNL 35
+LRL N DS H +TT SP H + + H+L
Sbjct: 118 QLRLGNQDSVHHLTTPASPTHHV--QNHHL 35
>TC14083 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor, partial
(92%)
Length = 1693
Score = 26.6 bits (57), Expect = 3.1
Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +1
Query: 130 FKNVRNVYDWKGLK-LEVDETHFDFGTLFEIECESSDPEEAKRILEE 175
FK+ ++Y + LK + ++ GTLF+ + DPE AK++ EE
Sbjct: 1015 FKDDPDLYVYPKLKYVGIELWQVKSGTLFDNVLITDDPEYAKQVAEE 1155
>BE122585
Length = 297
Score = 26.6 bits (57), Expect = 3.1
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +3
Query: 119 VGENGFVGLGGFKNVRNVYDWKGLK 143
+ G GL FKN+ V DW GL+
Sbjct: 3 IRHEGKYGLNFFKNLLRVLDWMGLR 77
>TC20215 similar to AAS10104 (AAS10104) MYB transcription factor, partial
(35%)
Length = 464
Score = 25.8 bits (55), Expect = 5.3
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +2
Query: 167 EEAKRILEEFLKENGIDYSYSVASKFAIFRAGK 199
EE +IL +F+K+NG S+ + R GK
Sbjct: 83 EEEDKILTDFIKQNGEGSWKSLPKNAGLLRCGK 181
>AV767982
Length = 508
Score = 25.4 bits (54), Expect = 7.0
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +1
Query: 9 LPNADSYHRVTTLLSPFHVITHRQHNLF 36
+ N S+ R+ S FHVI HR+ F
Sbjct: 352 MSNIKSFQRIQIRFSLFHVILHREVERF 435
>TC15958 similar to GB|AAP37838.1|30725632|BT008479 At4g24220 {Arabidopsis
thaliana;}, partial (52%)
Length = 800
Score = 25.0 bits (53), Expect = 9.1
Identities = 33/105 (31%), Positives = 46/105 (43%), Gaps = 8/105 (7%)
Frame = -2
Query: 43 ELSSRRAILRLRFYGDDERCVVSLKAKAVL-VDGVSRVEEDEEDLDPKIGRDCVDEPGKL 101
E+ R ++ L + ER V L A V + RV +D G + V E G +
Sbjct: 676 EILRERRLVELDLADELERADVVLTAGLERHVAEIGRVGDD--------GAEDVSEHGAV 521
Query: 102 GLVESRIMGRVK----EEFGVVGENGFVGLG--GFKNVR-NVYDW 139
L R V E+ G VGE+G +GLG G +V +V DW
Sbjct: 520 DLAILRFGVAVGPGDVEDVGDVGEDGELGLGVVGIGDVALDVVDW 386
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.141 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,992,288
Number of Sequences: 28460
Number of extensions: 37852
Number of successful extensions: 197
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of query: 200
length of database: 4,897,600
effective HSP length: 86
effective length of query: 114
effective length of database: 2,450,040
effective search space: 279304560
effective search space used: 279304560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144477.10