BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144474.2 - phase: 0
(201 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP041105 44 2e-05
TC14894 weakly similar to UP|Q9LD82 (Q9LD82) ESTs AU029648, part... 36 0.005
TC14508 similar to AAP86659 (AAP86659) 26S proteasome subunit RP... 29 0.49
AV766106 27 1.8
TC15384 weakly similar to UP|Q9LVB8 (Q9LVB8) HSR203J protein-lik... 27 3.2
TC11613 similar to UP|Q8W240 (Q8W240) GT-2 factor (Fragment), pa... 26 4.1
TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J... 26 4.1
TC15239 UP|Q852R1 (Q852R1) Serine palmitoyltransferase, partial ... 26 4.1
TC16603 similar to UP|Q7XJ86 (Q7XJ86) Glycinamide ribonucleotide... 25 7.0
TC7824 similar to UP|O04919 (O04919) Lipoxygenase , partial (58%) 25 7.0
TC8066 similar to UP|BAC81645 (BAC81645) Class1 chitinase (Fragm... 25 9.2
>BP041105
Length = 537
Score = 43.9 bits (102), Expect = 2e-05
Identities = 28/64 (43%), Positives = 35/64 (53%)
Frame = -3
Query: 135 EGGKKYGVVTFGGVASDEVVKEKVEKLRLCLEKDGFKVIGDFLLGRYNPPAITIPMFRTN 194
EGG V+ F G +++VV+EK + LR + KDG K LL RYN P T N
Sbjct: 526 EGGIA-AVIKFSGKPTEDVVREKEKTLRSNIMKDGLKPELGCLLARYNDPGRTWTFTMRN 350
Query: 195 EVLI 198
EVLI
Sbjct: 349 EVLI 338
>TC14894 weakly similar to UP|Q9LD82 (Q9LD82) ESTs AU029648, partial (84%)
Length = 982
Score = 35.8 bits (81), Expect = 0.005
Identities = 42/159 (26%), Positives = 72/159 (44%), Gaps = 7/159 (4%)
Frame = +3
Query: 11 ETPKYEILKTTQNYVIRKYAPSL-VAEITYDPSTFKGDKDGGFKVLVDYIGIFGKPQNTK 69
E P Y++++ Y IR+Y ++ ++ + GF L +YI + +N
Sbjct: 150 ECPSYDVIQVGNGYEIRRYNSTVWISNSPIQDISLVEATRTGFLRLFNYI----QGKNDY 317
Query: 70 TEKISMTTPVITKENKS------SSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEVP 123
++KI MT PV+++ + S SS ++ VP V N+ N P+ L V+
Sbjct: 318 SQKIEMTAPVLSEVSPSDGPFCESSFVVSFFVPKV-NQANPP-------PAKGLHVQRW- 470
Query: 124 KPIDERVVIREEGGKKYGVVTFGGVASDEVVKEKVEKLR 162
KP++ V +R+ FGG SD V E+ L+
Sbjct: 471 KPVN--VAVRQ----------FGGFVSDASVGEEAAALK 551
>TC14508 similar to AAP86659 (AAP86659) 26S proteasome subunit RPN5a,
partial (96%)
Length = 1957
Score = 29.3 bits (64), Expect = 0.49
Identities = 18/65 (27%), Positives = 35/65 (53%), Gaps = 9/65 (13%)
Frame = +2
Query: 115 MYLKVEEVPKPIDERVVIREEGG---------KKYGVVTFGGVASDEVVKEKVEKLRLCL 165
+Y+++E + I + I+EE G ++ V TFG +A E + +E++RLCL
Sbjct: 395 IYVEIERA-RLIKKLAKIKEEQGLIAEAADLMQEIAVETFGAMAKTEKIAFILEQVRLCL 571
Query: 166 EKDGF 170
++ +
Sbjct: 572 DRQDY 586
>AV766106
Length = 432
Score = 27.3 bits (59), Expect = 1.8
Identities = 17/64 (26%), Positives = 33/64 (51%)
Frame = +1
Query: 63 GKPQNTKTEKISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEV 122
G+ + KTEK + IT+ K + +M+ + +KN+++ + LPSM ++
Sbjct: 106 GQKFDFKTEKPNQNAYSITQN*KQRQKFSSMSKL*LRAQKNRVIAKKTPLPSMKNSGDDH 285
Query: 123 PKPI 126
KP+
Sbjct: 286 KKPL 297
>TC15384 weakly similar to UP|Q9LVB8 (Q9LVB8) HSR203J protein-like protein,
partial (21%)
Length = 1193
Score = 26.6 bits (57), Expect = 3.2
Identities = 27/121 (22%), Positives = 55/121 (45%), Gaps = 1/121 (0%)
Frame = +1
Query: 63 GKPQNTKTEKISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEV 122
G Q + + + + P + ++ SE + ++ N M+ + +LP + E
Sbjct: 631 GDKQVSNIQGLILVQPFFSGTLRTGSELRMVNDSHLSLCSNDML-WELSLPVGVNRDNEY 807
Query: 123 PKPIDERVVIREEGGKKYGV-VTFGGVASDEVVKEKVEKLRLCLEKDGFKVIGDFLLGRY 181
P +R E K+ G + G + D ++ +V +RL ++K+G +V+G F G Y
Sbjct: 808 CNPAVGNGPVRLEEIKRLGWRILVTGCSGDPLMDRQVGLVRL-MQKEGVRVVGHFTEGDY 984
Query: 182 N 182
+
Sbjct: 985 H 987
>TC11613 similar to UP|Q8W240 (Q8W240) GT-2 factor (Fragment), partial (26%)
Length = 731
Score = 26.2 bits (56), Expect = 4.1
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +1
Query: 59 IGIFGKPQN-TKTEKISMTTPVITKENKSSSEKIAMTVPVVTNEK 102
+G G P+ T++ + S +T +K+ S KIA VP+ TN K
Sbjct: 178 LGTTGMPRGATRSGRTSTSTSRRSKKATSRGVKIARHVPIFTNLK 312
>TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J8.110 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(29%)
Length = 771
Score = 26.2 bits (56), Expect = 4.1
Identities = 21/79 (26%), Positives = 35/79 (43%)
Frame = +3
Query: 83 ENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDERVVIREEGGKKYGV 142
ENK+ +EK +PV + + +P E K I V+ E G
Sbjct: 51 ENKNXTEK---AIPVTKKDGENPTPAKAEVPD-----NEFEKRIRPEVIPANEIG----- 191
Query: 143 VTFGGVASDEVVKEKVEKL 161
VTFG + + + +KE +++L
Sbjct: 192 VTFGDIGAMDEIKESLQEL 248
>TC15239 UP|Q852R1 (Q852R1) Serine palmitoyltransferase, partial (81%)
Length = 1896
Score = 26.2 bits (56), Expect = 4.1
Identities = 33/156 (21%), Positives = 61/156 (38%), Gaps = 14/156 (8%)
Frame = +2
Query: 59 IGIFGKPQNTKTEKISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLK 118
IG GK E + + T I + ++ + + + +++T P+ +L
Sbjct: 563 IGAVGKTGRGVRELLGVDTADIDIMMGTFTKSFGSCGGYIAGSQELIQYLKYTCPA-HLY 739
Query: 119 VEEVPKPIDE------RVVIREEGGKKYGVVTFGGVASDEVVKEKVEKLRLCLEKDGFKV 172
V P + +V++ E+G + G ++E R L+K GF+V
Sbjct: 740 ATSVSPPAAQQIISSIKVILGEDGSNR-------GAQKLAKIRENSNFFRSELQKMGFEV 898
Query: 173 IGD-----FLLGRYNP---PAITIPMFRTNEVLIPV 200
+GD + YNP PA + R N ++ V
Sbjct: 899 LGDNDSPVMPIMLYNPAKIPAFSRECLRQNVAVVTV 1006
>TC16603 similar to UP|Q7XJ86 (Q7XJ86) Glycinamide ribonucleotide
transformylase , partial (34%)
Length = 603
Score = 25.4 bits (54), Expect = 7.0
Identities = 13/42 (30%), Positives = 23/42 (53%)
Frame = +1
Query: 95 VPVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDERVVIREEG 136
VPV+ N+ + + + L VE V ++RVV+R++G
Sbjct: 154 VPVLANDTAEELAARVLREEHQLYVEAVEALCEDRVVLRKDG 279
>TC7824 similar to UP|O04919 (O04919) Lipoxygenase , partial (58%)
Length = 1675
Score = 25.4 bits (54), Expect = 7.0
Identities = 20/92 (21%), Positives = 43/92 (46%), Gaps = 7/92 (7%)
Frame = +2
Query: 60 GIFGKPQNTKT-EKISMTTPVITKENKSSSEKIAM-----TVPVVTNEKNKMVTMQFTLP 113
G++G +T T E+I + +T + ++K+ + T+ + N T +
Sbjct: 104 GVYGDHTSTITKEQIELNLDGLTTDQAIQNKKLFLLDHHDTIIPYLRQINSTTTKAYASR 283
Query: 114 S-MYLKVEEVPKPIDERVVIREEGGKKYGVVT 144
+ ++LK + KP+ + + G +YGVV+
Sbjct: 284 TILFLKSDGTLKPVAIELSLPNPQGDQYGVVS 379
>TC8066 similar to UP|BAC81645 (BAC81645) Class1 chitinase (Fragment),
complete
Length = 1392
Score = 25.0 bits (53), Expect = 9.2
Identities = 18/51 (35%), Positives = 25/51 (48%)
Frame = -3
Query: 150 SDEVVKEKVEKLRLCLEKDGFKVIGDFLLGRYNPPAITIPMFRTNEVLIPV 200
S+ V +V LE +VIGD + + PA+ P F T VL+PV
Sbjct: 352 SNSVSHRRVPLGEEILELVDLEVIGDGQVATVDGPALAFPAFVT--VLLPV 206
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.137 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,606,350
Number of Sequences: 28460
Number of extensions: 28452
Number of successful extensions: 146
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of query: 201
length of database: 4,897,600
effective HSP length: 86
effective length of query: 115
effective length of database: 2,450,040
effective search space: 281754600
effective search space used: 281754600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144474.2