BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144459.6 - phase: 0
(1105 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF177509 68 9e-12
BP030148 60 2e-09
TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-li... 45 5e-05
BP049972 43 3e-04
AV766619 42 4e-04
BP036178 41 0.001
TC17953 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17... 40 0.003
AV415611 39 0.004
BP083536 38 0.008
TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), par... 37 0.022
TC18180 weakly similar to GB|AAF04891.1|6175165|ATAC011437 Mutat... 34 0.11
AV765466 33 0.25
TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete 32 0.55
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 32 0.72
AV406521 31 0.94
TC9034 31 1.2
TC18767 31 1.2
TC19569 similar to UP|AAR11301 (AAR11301) Lectin-like receptor k... 30 1.6
TC9399 similar to UP|GBF_DICDI (P36417) G-box binding factor (GB... 30 2.1
AV778804 30 2.1
>BF177509
Length = 472
Score = 67.8 bits (164), Expect = 9e-12
Identities = 46/161 (28%), Positives = 71/161 (43%), Gaps = 2/161 (1%)
Frame = +1
Query: 586 DLGAWEWLMAVEPKKWCKHALPFYS--KCDVLMNXISESLNATILSARDQPIISMAEWIR 643
+ G W+ + P+ W A ++ + L I ESLN IL A PII M E IR
Sbjct: 1 EFGTRYWIRRIPPRLW---ATAYFEGQRFGHLTANIVESLNTWILEASGLPIIQMMECIR 171
Query: 644 HYLMRRMTTSATKLQKWQHNVMPMPRKRLDKEITLAAHWRSTWSGIGEQFQVMHTYNRQQ 703
LM +W ++P + + + + A ++ + E + H +
Sbjct: 172 RQLMTWFNERRETSMQWASILVPSAERCVAEALERARTYQVLRANEAEFEVISHEGSN-- 345
Query: 704 FIVDIAKRSCSCNFWEIVGIPCRHAVSALGKRKQRPEMFVD 744
IVDI R C C W++ G+PC HAV+AL +Q F +
Sbjct: 346 -IVDIRNRCCLCRGWQLYGLPCAHAVAALLSCRQNVHRFTE 465
>BP030148
Length = 398
Score = 60.1 bits (144), Expect = 2e-09
Identities = 40/131 (30%), Positives = 66/131 (49%), Gaps = 2/131 (1%)
Frame = +3
Query: 514 RYVFISDQQKGLLSVFDEMFDTIDHRLCLRHLYANFKKKFGGGSQIRDLMMGAAKATYYQ 573
R +SD+QKG++ + F T H C+RHL +F+K+F + + +L+ AA
Sbjct: 18 RLTILSDRQKGIVDGVEANFPTAFHGFCMRHLSDSFRKEF-NNTMLVNLLWEAAHVLTVI 194
Query: 574 GWLEKMNELKKIDLGAWEWLMAVEPKKWCKHALPFY--SKCDVLMNXISESLNATILSAR 631
+ K+ E+++I A W+ + P+ W A ++ + L I ESL+ IL A
Sbjct: 195 EFESKILEIEEISQDAAYWIRRIPPRLW---ATAYFEGQRFGHLTANIVESLDTWILDAS 365
Query: 632 DQPIISMAEWI 642
PI+ M E I
Sbjct: 366 GLPIVQMTECI 398
>TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-like
transposase {Arabidopsis thaliana;} , partial (12%)
Length = 588
Score = 45.4 bits (106), Expect = 5e-05
Identities = 29/89 (32%), Positives = 44/89 (48%), Gaps = 10/89 (11%)
Frame = +3
Query: 752 YALCYSFAISPINGMDMWPEV--------EAPELL--PPNYKNGPGRPRKLRIREFDENG 801
Y YS I PI +W E+ +A ++L PP PGRPRK R+R E+
Sbjct: 27 YRKTYSQTIHPIPDKSLWKELSEGDENAAKADQVLINPPKSLRPPGRPRKKRVRA--EDR 200
Query: 802 SRMRRQGVAYRCTKCDQFGHNQRRCKSDV 830
R++R C++C+Q GH + C + +
Sbjct: 201 GRVKR---VVHCSRCNQTGHFRTTCAAPI 278
>BP049972
Length = 496
Score = 42.7 bits (99), Expect = 3e-04
Identities = 28/108 (25%), Positives = 44/108 (39%), Gaps = 19/108 (17%)
Frame = -1
Query: 742 FVDDYYSRTKYALCYSFAISPINGMDMWPEVEAPE-------------------LLPPNY 782
F + ++ Y YS I PI W E+ + + PP
Sbjct: 490 FTESCFTVATYRKTYSQTIHPIPDRSFWNELSIEDANANKAVEDANASQSIEIVINPPKS 311
Query: 783 KNGPGRPRKLRIREFDENGSRMRRQGVAYRCTKCDQFGHNQRRCKSDV 830
PGRPRK R+R E+ R++R C++C+Q GH + C + +
Sbjct: 310 LRPPGRPRKKRVRA--EDRGRVKR---VVHCSRCNQTGHFRTTCAAPI 182
>AV766619
Length = 412
Score = 42.4 bits (98), Expect = 4e-04
Identities = 27/99 (27%), Positives = 43/99 (43%), Gaps = 6/99 (6%)
Frame = +2
Query: 509 IGQDKRYVFISDQQKGLLSVFDEMFDTIDHRLCLRHLYANFKKKFGG--GSQIRDLMMG- 565
I + F+++ Q GL E+F+ H CLR+L K G + R M+
Sbjct: 2 ISTSEPITFVANCQNGLKKSLSEIFEKCHHSYCLRYLAEKLNKDLKGQFSHEARRFMIND 181
Query: 566 ---AAKATYYQGWLEKMNELKKIDLGAWEWLMAVEPKKW 601
AA A + + + +K I A+ W+M EP+ W
Sbjct: 182 FYAAAYAPKQETFERSVENMKGISSEAFNWVMQNEPEHW 298
>BP036178
Length = 561
Score = 41.2 bits (95), Expect = 0.001
Identities = 28/86 (32%), Positives = 39/86 (44%), Gaps = 11/86 (12%)
Frame = -2
Query: 752 YALCYSFAISPINGMDMWPEV-EAPE----------LLPPNYKNGPGRPRKLRIREFDEN 800
Y + YS I PI W E E E + PP + PGRP+K +R EN
Sbjct: 560 YRMAYSQIIIPIPDRSQWREHGEGAEGVGGARVDIVIRPPKTRRPPGRPKKKVLRV--EN 387
Query: 801 GSRMRRQGVAYRCTKCDQFGHNQRRC 826
R +R +C +C GH+Q++C
Sbjct: 386 FKRPKR---IVQCGRCHMLGHSQKKC 318
>TC17953 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;}, partial (6%)
Length = 527
Score = 39.7 bits (91), Expect = 0.003
Identities = 19/57 (33%), Positives = 31/57 (54%)
Frame = +3
Query: 774 APELLPPNYKNGPGRPRKLRIREFDENGSRMRRQGVAYRCTKCDQFGHNQRRCKSDV 830
A + PP K PGRP+ + D ++RQ +C+KC + GHN++ CK+ +
Sbjct: 27 AVTVTPPPTKRPPGRPKMKPVESID----MIKRQ---LQCSKCKELGHNKKTCKNSL 176
>AV415611
Length = 219
Score = 38.9 bits (89), Expect = 0.004
Identities = 12/28 (42%), Positives = 20/28 (70%)
Frame = +3
Query: 705 IVDIAKRSCSCNFWEIVGIPCRHAVSAL 732
+VDI + CSC W++ G+PC HA++ +
Sbjct: 108 VVDIDRWECSCKTWQLTGVPCCHAIAVI 191
>BP083536
Length = 377
Score = 38.1 bits (87), Expect = 0.008
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = +1
Query: 711 RSCSCNFWEIVGIPCRHAVSALGKR 735
++C CN WE+V IPCRHA++ + R
Sbjct: 301 KACCCNLWELVEIPCRHALAGVHYR 375
>TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), partial (76%)
Length = 1277
Score = 36.6 bits (83), Expect = 0.022
Identities = 29/125 (23%), Positives = 47/125 (37%)
Frame = +2
Query: 833 PEAAKRKRKTPRRKNTSTATEPMQTDPMQAEVAAATEPMQAEVEAGTEPMQTEPIQTDPM 892
P AAK K K + + A + P AAAT+ A + +P
Sbjct: 506 PAAAKSKPKPKAKAKPAAAATKAKAKPAPKSKAAATKTTAKAKPAAAAKPKAKP----AA 673
Query: 893 QAEVAAGTKPMQAEVEAGTEPMQPEVEAGTQPMHTASTQDPPRHTTKIVKVKNSEAPFKI 952
+A+ AA K + A +A P +P+ +A ++ + P K K + P K
Sbjct: 674 KAKPAAKAKAVAAPAKAKASPAKPKAKAKSKTAPRMNVNPPVPKAKPAAKAKPAARPAKA 853
Query: 953 QKVVT 957
+ T
Sbjct: 854 SRTST 868
>TC18180 weakly similar to GB|AAF04891.1|6175165|ATAC011437 Mutator-like
transposase {Arabidopsis thaliana;} , partial (4%)
Length = 513
Score = 34.3 bits (77), Expect = 0.11
Identities = 29/93 (31%), Positives = 44/93 (47%), Gaps = 8/93 (8%)
Frame = +3
Query: 743 VDDYYSR----TKYALCYSFAISPINGMDMWPEVEAPE----LLPPNYKNGPGRPRKLRI 794
V DY SR Y L YS ++PI M + PE + + + PP + PGRP R
Sbjct: 6 VYDYCSRYCTTESYRLTYSERVNPIPIMAV-PESKDSQPVVTVTPPFTRRPPGRPATKRY 182
Query: 795 REFDENGSRMRRQGVAYRCTKCDQFGHNQRRCK 827
+ + ++R+ C++C GHN+ CK
Sbjct: 183 ----VSQNIVKRE---LHCSRCKGLGHNKSTCK 260
>AV765466
Length = 599
Score = 33.1 bits (74), Expect = 0.25
Identities = 21/74 (28%), Positives = 37/74 (49%), Gaps = 2/74 (2%)
Frame = -2
Query: 713 CSCNFWEIVGIPCRHAVSALGKR--KQRPEMFVDDYYSRTKYALCYSFAISPINGMDMWP 770
C C +E GI CRH+++ L + K+ P+ +V D + R Y + + + P
Sbjct: 592 CDCRLFEFRGILCRHSLAILSQERVKEVPDRYVLDRW-RKNMRRKYVYVKTSYCVQHLKP 416
Query: 771 EVEAPELLPPNYKN 784
++E ELL +K+
Sbjct: 415 QMERLELLCNQFKS 374
>TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete
Length = 999
Score = 32.0 bits (71), Expect = 0.55
Identities = 40/169 (23%), Positives = 65/169 (37%)
Frame = +1
Query: 853 EPMQTDPMQAEVAAATEPMQAEVEAGTEPMQTEPIQTDPMQAEVAAGTKPMQAEVEAGTE 912
EP + +Q + +P EV A +P AEV A +P E + T
Sbjct: 139 EPTEVTKIQETIPEPEQPAATEVAAPEQPA-----------AEVPAPAEPATEEPKEETT 285
Query: 913 PMQPEVEAGTQPMHTASTQDPPRHTTKIVKVKNSEAPFKIQKVVTSEAANALPEKIVTEF 972
E E T+ TQDP ++V+N E VT EA PE++ E
Sbjct: 286 EEPKETETETEAPVAPETQDP-------LEVENKE--------VTEEAK---PEEVKPET 411
Query: 973 YEALPESQLETSLGPDVFDDLDDSVLAAITDQMFEAAEKEFNVVNVVKS 1021
+ +++ + P + + + A + ++ A E VV V K+
Sbjct: 412 EKTEEKTEEPKTEEPAATTETESA--APVEEENKPAEEAPVEVVPVEKT 552
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 31.6 bits (70), Expect = 0.72
Identities = 18/40 (45%), Positives = 23/40 (57%)
Frame = +3
Query: 121 DEEDVMVSTDDDSGKFVGFDDSEDERTTALEDGFEDVEVE 160
DE+D DDD G+ DD +DE E+G E+VEVE
Sbjct: 291 DEDDDDDDDDDDDGEEEEDDDDDDEE----EEGSEEVEVE 398
>AV406521
Length = 267
Score = 31.2 bits (69), Expect = 0.94
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 4/45 (8%)
Frame = +1
Query: 485 LAFGVVETECKESWKWFIQLLMEDIGQDKRYV----FISDQQKGL 525
LAFGVV+ E ++W WF L + + + F+SD Q+G+
Sbjct: 1 LAFGVVDVENDDNWTWFRSELHKALEASTESMPQMGFLSDGQRGI 135
>TC9034
Length = 583
Score = 30.8 bits (68), Expect = 1.2
Identities = 21/68 (30%), Positives = 29/68 (41%), Gaps = 2/68 (2%)
Frame = +1
Query: 780 PNYKNGPGRPRKLRIREFDENG--SRMRRQGVAYRCTKCDQFGHNQRRCKSDVQNPEAAK 837
P K PG R R R E S R+ +C C++FGH+ C+ D +K
Sbjct: 43 PTLKRLPGWERINRKRGVTEGPTVSHEARRSHRVKCANCEEFGHDSWECQRD-----KSK 207
Query: 838 RKRKTPRR 845
R+ K R
Sbjct: 208 RQEKVTVR 231
>TC18767
Length = 1004
Score = 30.8 bits (68), Expect = 1.2
Identities = 14/43 (32%), Positives = 23/43 (52%), Gaps = 2/43 (4%)
Frame = +2
Query: 810 AYRCTKCDQFGHNQRRCKSDVQN--PEAAKRKRKTPRRKNTST 850
A RC C + H+ R C N +A+++RK+ R +N S+
Sbjct: 158 ASRCFNCGSYNHSLRECSRPRDNVAVNSARKQRKSRRNQNVSS 286
>TC19569 similar to UP|AAR11301 (AAR11301) Lectin-like receptor kinase 1;1,
partial (16%)
Length = 486
Score = 30.4 bits (67), Expect = 1.6
Identities = 25/122 (20%), Positives = 42/122 (33%), Gaps = 14/122 (11%)
Frame = +1
Query: 833 PEAAKRKRKTPRRKNTSTATEP-----MQTDPMQAEVAAATEPMQAEVEAGTEPMQTEPI 887
P +++ +P TST T P + +P A + P + E + P +
Sbjct: 79 PPPSQQPTHSPSTSPTSTTTPPPSPTKVTENPPTAPSTSTKSPTSSASEEPSPPNPSTST 258
Query: 888 QTDPMQAEVAA---------GTKPMQAEVEAGTEPMQPEVEAGTQPMHTASTQDPPRHTT 938
P + + TKP A T P+ T P +++ PP TT
Sbjct: 259 TQQPKHSPTSPPASPSPSPPSTKPPTAMASPSTSPLSATKSHPTPPAAPSASSTPPPTTT 438
Query: 939 KI 940
+
Sbjct: 439 SL 444
>TC9399 similar to UP|GBF_DICDI (P36417) G-box binding factor (GBF),
partial (3%)
Length = 718
Score = 30.0 bits (66), Expect = 2.1
Identities = 15/49 (30%), Positives = 25/49 (50%)
Frame = +3
Query: 4 FDVVFHHGGEFVMDKRIFYRGGVQTVVSGLESNNWGVCDIQNTVASWGY 52
F + HH G+FV + R+ Y V++ E++ W + + VA GY
Sbjct: 510 FKAIIHHSGQFVEEPRLHY-VDVKSNDVHAETDRWSFFECLDVVAEVGY 653
>AV778804
Length = 573
Score = 30.0 bits (66), Expect = 2.1
Identities = 14/49 (28%), Positives = 26/49 (52%)
Frame = +1
Query: 823 QRRCKSDVQNPEAAKRKRKTPRRKNTSTATEPMQTDPMQAEVAAATEPM 871
+ R +S + + K+K++ P+ +N Q + MQ+ AAA EP+
Sbjct: 130 KNRVESWKEKDKKKKKKKEAPKAENEMPIPPEQQMEEMQSTEAAAAEPL 276
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.134 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,661,409
Number of Sequences: 28460
Number of extensions: 238410
Number of successful extensions: 1295
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 1264
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1289
length of query: 1105
length of database: 4,897,600
effective HSP length: 100
effective length of query: 1005
effective length of database: 2,051,600
effective search space: 2061858000
effective search space used: 2061858000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144459.6