BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144459.2 - phase: 0 /pseudo
(824 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19518 similar to GB|CAC05466.1|9955527|ATT5E8 potassium transp... 262 2e-70
CB828967 159 2e-39
AW719689 124 5e-29
AV425381 112 2e-25
BP042082 112 2e-25
AV427785 79 3e-15
TC10952 similar to UP|PT11_ARATH (O64769) Potassium transporter ... 73 2e-13
TC19918 weakly similar to UP|Q9AYN4 (Q9AYN4) High-affinity potas... 49 4e-06
BP058734 34 0.082
TC16036 homologue to GB|BAB02032.1|9294075|AB020749 cytosolic ch... 32 0.31
TC14667 homologue to UP|Q84P43 (Q84P43) Protein kinase Pti1, com... 30 2.0
TC17050 similar to UP|Q9LTG1 (Q9LTG1) Gb|AAD38661.1, partial (48%) 29 3.5
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 29 3.5
BP036242 28 5.9
TC10688 similar to UP|AAR10812 (AAR10812) Superoxide dismutase, ... 28 5.9
BP035812 28 7.7
AV776603 28 7.7
TC18053 weakly similar to GB|AAH54244.1|32484237|BC054244 MGC644... 28 7.7
TC8327 UP|VATL_PHAAU (O22552) Vacuolar ATP synthase 16 kDa prote... 28 7.7
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 28 7.7
>TC19518 similar to GB|CAC05466.1|9955527|ATT5E8 potassium transport
protein-like {Arabidopsis thaliana;} , partial (15%)
Length = 541
Score = 262 bits (669), Expect = 2e-70
Identities = 131/163 (80%), Positives = 142/163 (86%)
Frame = +3
Query: 662 FEQLLMESLEKFIRREAQERSLESDGDEDTELEDEYAGSRVLIAPNGSVYSLGVPLLADF 721
FEQLL+ESLEKF+RRE QERSLESDGDED+E EDEY+GSRVLIAPNGSVYSLG+PLLADF
Sbjct: 9 FEQLLIESLEKFVRREVQERSLESDGDEDSEQEDEYSGSRVLIAPNGSVYSLGIPLLADF 188
Query: 722 NESFMPSFEPSTSEEAGPPSPKPLVLDAEQLLERELSFIRNAKESGLVYLLGHGDIRARK 781
++ P E STSE +P V DAEQ LERELSFIR AKESG+VYLLGHGDIRARK
Sbjct: 189 TDTINPVLEASTSEVVNSTTPDHPVFDAEQGLERELSFIRKAKESGVVYLLGHGDIRARK 368
Query: 782 DSWFIKKLVINYFYAFLRKNCRRGVTNLSVPHSHLMQVGMTYM 824
DSWFIKKLVINYFYAFLRKNCRRG+T LSVPHS+LMQV MTYM
Sbjct: 369 DSWFIKKLVINYFYAFLRKNCRRGITTLSVPHSNLMQVSMTYM 497
>CB828967
Length = 526
Score = 159 bits (402), Expect = 2e-39
Identities = 75/164 (45%), Positives = 105/164 (63%)
Frame = +1
Query: 522 LWSITDGSWIILVFAAIMFFIMFIWNYGSKLKYETEVKQKLSPDLMRELGCNLGTIRAPG 581
L + G W+ LV + I IM++W+YG++ KY +++ K+S + LG +LG +R PG
Sbjct: 4 LIKVHQGGWVPLVLSFIFLVIMYVWHYGTRKKYSYDLRNKVSLKWLLALGPSLGIVRVPG 183
Query: 582 IGLLYNELVKGIPGIFGHFLTTLPAIHSMIIFVSIKYVPVAMVPQSERFLFRRVCQRSYH 641
IGL+Y EL GIP IF HF+T LPA H +++FV +K VPV V ERFL R C R Y
Sbjct: 184 IGLIYTELATGIPSIFSHFVTNLPAFHQVLVFVCVKSVPVPHVSSEERFLIGRACPRPYR 363
Query: 642 LFRCIARYGYKDARKENHQAFEQLLMESLEKFIRREAQERSLES 685
++RCI RYGYKD +K++ FE L++S+ +FI EA E S
Sbjct: 364 MYRCIVRYGYKDIKKDDGD-FENHLIQSIAEFIVMEAAESQFSS 492
>AW719689
Length = 576
Score = 124 bits (312), Expect = 5e-29
Identities = 68/183 (37%), Positives = 111/183 (60%)
Frame = +1
Query: 101 IVLAFQTLGVVFGDVGTSPLYTFSVMFRKAPINDNEDILGALSLVLYTLILIPFLKYVLV 160
++LA++TLGVVFG + TSPLY + M +P ED LG S++ +TL LI +KY +
Sbjct: 34 LILAYRTLGVVFGGLVTSPLYVYPSMPLHSPTE--EDYLGIYSIIFWTLTLIGVVKYANI 207
Query: 161 VLWANDDGEGGTFALYSLICRNAKVNLLPNQLPSDARISGFRLKVPSAELERSLKLKERL 220
+ A+D GEGGTFA+YSL+CR+ + +LP++ + G L + E L +
Sbjct: 208 AIKADDHGEGGTFAVYSLLCRHFNIGILPSKQQQQQQQLG--LNSMTRGTETHTWLAKLF 381
Query: 221 ESSFTLKKILLLLVLAGTSMVIANGVVTPAMSVLSSVNGLKVGVDAIQQDEVVMISVACL 280
E S +++LL + + GT M+I +G++TPA+SVLS+++G++ + + V +S L
Sbjct: 382 ERSVVARRVLLFVAMLGTCMLIGDGILTPAISVLSAMDGVRAPFPKVSKSLVEALSAGXL 561
Query: 281 VVL 283
+ L
Sbjct: 562 IFL 570
>AV425381
Length = 328
Score = 112 bits (281), Expect = 2e-25
Identities = 50/105 (47%), Positives = 71/105 (67%)
Frame = +2
Query: 517 FFSSVLWSITDGSWIILVFAAIMFFIMFIWNYGSKLKYETEVKQKLSPDLMRELGCNLGT 576
+FS+VL+ I G W L A IM++W+YG+ +YE E+ K+S + LG +LG
Sbjct: 8 YFSAVLFKIDQGGWAPLAIAGAFLIIMYVWHYGTVKRYEFEMHSKVSMAWILGLGPSLGL 187
Query: 577 IRAPGIGLLYNELVKGIPGIFGHFLTTLPAIHSMIIFVSIKYVPV 621
+R PG+GL+Y EL G+P IF HF+T LPAIHS+++FV KY+PV
Sbjct: 188 VRVPGVGLVYTELASGVPHIFSHFITNLPAIHSVLVFVCEKYLPV 322
>BP042082
Length = 521
Score = 112 bits (281), Expect = 2e-25
Identities = 59/134 (44%), Positives = 90/134 (67%), Gaps = 2/134 (1%)
Frame = +3
Query: 101 IVLAFQTLGVVFGDVGTSPLYTFSVMF--RKAPINDNEDILGALSLVLYTLILIPFLKYV 158
+ LA+Q+LGVV+GD+ TSPLY + F + D+E+I G LS + +T +I KYV
Sbjct: 120 LTLAYQSLGVVYGDLSTSPLYVYKTTFSGKLRLKEDDEEIFGVLSFIFWTFTIIALFKYV 299
Query: 159 LVVLWANDDGEGGTFALYSLICRNAKVNLLPNQLPSDARISGFRLKVPSAELERSLKLKE 218
+V+ A+D+GEGGTFALYSL+CR+A++++LPNQ P+DA++S + + S + +S LK
Sbjct: 300 FIVMSADDNGEGGTFALYSLLCRHARLSILPNQQPTDAQLSSYATE-NSEDTWQSSFLKM 476
Query: 219 RLESSFTLKKILLL 232
E +K LL+
Sbjct: 477 FFEKHPRFQKGLLI 518
>AV427785
Length = 254
Score = 79.0 bits (193), Expect = 3e-15
Identities = 36/82 (43%), Positives = 58/82 (69%)
Frame = +2
Query: 444 YIPVINWFLLAVSLVFVCTISSIDEIGNAYGIAELGVMMMTTILVTLVMLLIWQMHIIIV 503
YIP INW L+ + + + ++IGNAYG A + VM++TT+L+ L+MLL+W+ H I+V
Sbjct: 2 YIPDINWILMILCIAVTAGFKNQNQIGNAYGTAVVIVMLVTTLLMILIMLLVWRCHWILV 181
Query: 504 MSFLGVFLGLELVFFSSVLWSI 525
++F G+ L +E +FS+VL+ I
Sbjct: 182 VAFTGLSLIVECTYFSAVLFKI 247
>TC10952 similar to UP|PT11_ARATH (O64769) Potassium transporter 11
(AtPOT11), partial (9%)
Length = 632
Score = 72.8 bits (177), Expect = 2e-13
Identities = 42/121 (34%), Positives = 66/121 (53%), Gaps = 4/121 (3%)
Frame = +1
Query: 707 NGSVYSLGVPLLADFNESFMPSFEPS----TSEEAGPPSPKPLVLDAEQLLERELSFIRN 762
+G+ S V L +S +P+ PS T + +G S V EL FI +
Sbjct: 49 HGNTASSNVDLSISSVDSILPARSPSNVHVTFQSSGHGSHNTEV--------DELEFINS 204
Query: 763 AKESGLVYLLGHGDIRARKDSWFIKKLVINYFYAFLRKNCRRGVTNLSVPHSHLMQVGMT 822
+++G+V++LG+ +RAR+DS F KK+ ++Y YAFLRK CR ++PH L+ VG
Sbjct: 205 CRDAGVVHILGNTVVRARRDSRFYKKIAVDYIYAFLRKVCREHSVIFNIPHESLLNVGQV 384
Query: 823 Y 823
+
Sbjct: 385 F 387
>TC19918 weakly similar to UP|Q9AYN4 (Q9AYN4) High-affinity potassium
transporter, partial (5%)
Length = 423
Score = 48.5 bits (114), Expect = 4e-06
Identities = 20/36 (55%), Positives = 29/36 (80%)
Frame = +2
Query: 788 KLVINYFYAFLRKNCRRGVTNLSVPHSHLMQVGMTY 823
K+V+NY Y+FLRKN R+G L++P + L++VGMTY
Sbjct: 2 KIVVNYAYSFLRKNFRQGENLLAIPRNRLLKVGMTY 109
>BP058734
Length = 453
Score = 34.3 bits (77), Expect = 0.082
Identities = 15/50 (30%), Positives = 31/50 (62%)
Frame = +3
Query: 490 LVMLLIWQMHIIIVMSFLGVFLGLELVFFSSVLWSITDGSWIILVFAAIM 539
L++LL+ M +++++SFLG+ G ++ F +LW S +L+ A++
Sbjct: 252 LLLLLLLLMMVLLLLSFLGLLGGFLMLIFLVILWLWLSASGFLLLHHALL 401
>TC16036 homologue to GB|BAB02032.1|9294075|AB020749 cytosolic chaperonin,
delta-subunit {Arabidopsis thaliana;} , partial (14%)
Length = 575
Score = 32.3 bits (72), Expect = 0.31
Identities = 20/49 (40%), Positives = 29/49 (58%), Gaps = 1/49 (2%)
Frame = +1
Query: 501 IIVMSFLGVFLGLEL-VFFSSVLWSITDGSWIILVFAAIMFFIMFIWNY 548
II++SFL + L L + V FS I D W++L+FA + IM IW +
Sbjct: 283 IIMLSFLALILKLLVYVPFSDTG*VIFDLLWLVLLFANLDVLIMCIWEF 429
>TC14667 homologue to UP|Q84P43 (Q84P43) Protein kinase Pti1, complete
Length = 1591
Score = 29.6 bits (65), Expect = 2.0
Identities = 16/44 (36%), Positives = 23/44 (51%), Gaps = 3/44 (6%)
Frame = -2
Query: 320 YDSSVFRAFNPIHIYYFFARNSTKAWYSLGG---CLLCATGSEA 360
YD S AF+P + + + A S+GG C+ C TG+EA
Sbjct: 393 YDPSPINAFDPKLSVFSLSSSKDNAGTSIGGGAFCVSCLTGAEA 262
>TC17050 similar to UP|Q9LTG1 (Q9LTG1) Gb|AAD38661.1, partial (48%)
Length = 1011
Score = 28.9 bits (63), Expect = 3.5
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
Frame = -2
Query: 397 LFFFCSK--LIASRTMTTATFSCIKQSTALGCFPR 429
L+ FC +AS + ATFSC K T + C P+
Sbjct: 494 LYLFCGSNASLASLSSKHATFSCFKSVTCVLCMPK 390
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 28.9 bits (63), Expect = 3.5
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = +2
Query: 33 QEDEDPSEIEDYDASDMRHQSMFDSEDEDNAE 64
++DED E ED D D + D +DED+ E
Sbjct: 776 EDDEDGDEDEDEDDDDDEEEEDDDDDDEDDEE 871
>BP036242
Length = 525
Score = 28.1 bits (61), Expect = 5.9
Identities = 13/29 (44%), Positives = 18/29 (61%)
Frame = +3
Query: 34 EDEDPSEIEDYDASDMRHQSMFDSEDEDN 62
+DED E++DY ASD+ EDED+
Sbjct: 276 DDEDAEELDDYGASDL------SDEDEDD 344
>TC10688 similar to UP|AAR10812 (AAR10812) Superoxide dismutase, partial
(91%)
Length = 822
Score = 28.1 bits (61), Expect = 5.9
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = +2
Query: 706 PNGSVYSLGVPLLADFNESFMPSFEPSTSEEAGPPSPKP 744
P S +SL +D PS P TSE + PP+P P
Sbjct: 59 PTRSSHSLLTHHSSDHPSPVPPSNSPPTSEPSHPPNPSP 175
>BP035812
Length = 572
Score = 27.7 bits (60), Expect = 7.7
Identities = 14/53 (26%), Positives = 24/53 (44%)
Frame = +1
Query: 13 EEDDPGSALSFDSTESRWVFQEDEDPSEIEDYDASDMRHQSMFDSEDEDNAEM 65
EED + DS S W + D E ++ D + + + D + +D+ EM
Sbjct: 412 EEDSNEDSNMDDSAVSSWDGTDSNDLDEEQESDGENQEYATALDVDFDDSVEM 570
>AV776603
Length = 359
Score = 27.7 bits (60), Expect = 7.7
Identities = 15/40 (37%), Positives = 20/40 (49%)
Frame = -1
Query: 302 LFIWFCSLAGNGVYNLVKYDSSVFRAFNPIHIYYFFARNS 341
L W CS G + L Y S V ++P ++YF RNS
Sbjct: 131 LIFWHCSNQGTPLQKL*LY-SIVRTGYHPNRLFYFSMRNS 15
>TC18053 weakly similar to GB|AAH54244.1|32484237|BC054244 MGC64450 protein
{Xenopus laevis;} , partial (20%)
Length = 704
Score = 27.7 bits (60), Expect = 7.7
Identities = 12/39 (30%), Positives = 25/39 (63%), Gaps = 1/39 (2%)
Frame = +1
Query: 657 ENHQAFE-QLLMESLEKFIRREAQERSLESDGDEDTELE 694
E ++AF+ + + EK++RRE + S +D ED++++
Sbjct: 529 ERNEAFDFNVALSDHEKYVRREHDKESGNADAAEDSQID 645
>TC8327 UP|VATL_PHAAU (O22552) Vacuolar ATP synthase 16 kDa proteolipid
subunit , complete
Length = 889
Score = 27.7 bits (60), Expect = 7.7
Identities = 14/57 (24%), Positives = 28/57 (48%)
Frame = +1
Query: 439 FMGQIYIPVINWFLLAVSLVFVCTISSIDEIGNAYGIAELGVMMMTTILVTLVMLLI 495
F G P + A +LVF C ++ + G+A +GVM ++ ++V +++
Sbjct: 109 FSGDETAPFFGFLGAAAALVFSCMGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVM 279
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 27.7 bits (60), Expect = 7.7
Identities = 11/30 (36%), Positives = 18/30 (59%), Gaps = 3/30 (10%)
Frame = -2
Query: 365 LCYFSVRSVQPIIGLFGSSCIPYGT---PC 391
LCYFS++S+ P++ F + + T PC
Sbjct: 620 LCYFSIQSINPLLSFFCFTLLSINTSLSPC 531
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.141 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,312,434
Number of Sequences: 28460
Number of extensions: 212650
Number of successful extensions: 1574
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 1545
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1569
length of query: 824
length of database: 4,897,600
effective HSP length: 98
effective length of query: 726
effective length of database: 2,108,520
effective search space: 1530785520
effective search space used: 1530785520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC144459.2