BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144389.8 - phase: 0
(468 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI418387 160 4e-40
TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase... 56 1e-08
TC9659 homologue to UP|Q9SEA8 (Q9SEA8) Salt-induced AAA-Type ATP... 49 2e-06
TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {... 48 3e-06
TC11760 homologue to UP|PRS4_ORYSA (P46466) 26S protease regulat... 38 0.004
TC9051 UP|PRS7_ARATH (Q9SSB5) 26S protease regulatory subunit 7 ... 36 0.015
TC14829 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulat... 35 0.026
AV422700 34 0.058
TC12379 homologue to UP|O04327 (O04327) Cell division protein Ft... 34 0.058
TC13255 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (41%) 33 0.13
AW719510 33 0.13
TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J... 32 0.17
AV421390 32 0.29
TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulat... 32 0.29
TC11791 UP|PRSA_LYCES (P54776) 26S protease regulatory subunit 6... 30 1.1
TC15037 similar to UP|O24457 (O24457) Pyruvate dehydrogenase E1 ... 29 1.4
AV420640 28 2.4
CN825587 28 3.2
TC14911 homologue to UP|Q93X55 (Q93X55) Peroxin 6 (Fragment), pa... 28 3.2
CN825067 28 3.2
>BI418387
Length = 479
Score = 160 bits (405), Expect = 4e-40
Identities = 83/156 (53%), Positives = 105/156 (67%)
Frame = +1
Query: 1 MVIFIYTPILLKIAILLFSIYAIHMIFETGLIHESTKLWRIIEDWFHVYQVFHVPELNDN 60
MV+F+ PIL+ + ++ + I + WR I+DWFHVYQ F VP LN
Sbjct: 52 MVLFLCNPILVLLTPFFLFLFLLLSI----------RWWRPIQDWFHVYQFFRVPLLNQT 201
Query: 61 MQHNTLYRKLSLYFHSLPSLQNSQLNNLVTSNTNQNDVVLTLAPNQTIHDHFLGATVSWF 120
MQHN LYR LSLY HSLPS+Q+S +NL+T T Q+DV+L+L PNQTIHDHFLGATVSW
Sbjct: 202 MQHNHLYRNLSLYLHSLPSIQDSDFSNLLTGPT-QHDVILSLGPNQTIHDHFLGATVSWS 378
Query: 121 NQTQPNRTFILRIRKFDKQRILRAYIQHIHAVVDEI 156
N+ F+L+IRK D++RILR Y+ HI AV EI
Sbjct: 379 NEP---TAFVLKIRKSDRRRILRPYLHHISAVAAEI 477
>TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase subunit
RPT2a, partial (69%)
Length = 916
Score = 56.2 bits (134), Expect = 1e-08
Identities = 47/167 (28%), Positives = 72/167 (42%), Gaps = 22/167 (13%)
Frame = +1
Query: 200 VKSDLESFLKGKQYYHRLGRLWKRSFLLYGSSGTGKSSFIAAMANFLSYDVYYIDLSRIS 259
+K +E L + Y +G + +LYG GTGK+ A+AN S +
Sbjct: 418 IKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTSATFLRV------ 579
Query: 260 TDSDLKSILLQTAPK--------------SIIVVEDLDRYLTEKSSTTV--------TSS 297
S+L L PK SI+ ++++D T++ T
Sbjct: 580 VGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHSGGEREIQRTML 759
Query: 298 GILNFMDGIWSGEERVMVFTMNSKENVDPNLLRPGRVDVHIHFPLCD 344
+LN +DG S + ++ N E++DP LLRPGR+D I FPL D
Sbjct: 760 ELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPD 900
>TC9659 homologue to UP|Q9SEA8 (Q9SEA8) Salt-induced AAA-Type ATPase,
partial (74%)
Length = 1060
Score = 48.5 bits (114), Expect = 2e-06
Identities = 46/172 (26%), Positives = 80/172 (45%), Gaps = 16/172 (9%)
Frame = +1
Query: 208 LKGKQYYHRLGRLWKRSFLLYGSSGTGKSSFIAAMANFLSYDVYYIDLSRISTD------ 261
+K Q++ R W R+FLLYG GTGKS A+A + + S + +
Sbjct: 523 VKFPQFFTGKRRPW-RAFLLYGPPGTGKSYLAKAVATEADSTFFSVSSSDLGSKWMGESE 699
Query: 262 ---SDLKSILLQTAPKSIIVVEDLDRYL------TEKSSTTVTSSGILNFMDGIWSGEER 312
S+L + ++AP SII V+++D E ++ + +L M G+ + +++
Sbjct: 700 KLVSNLFQMARESAP-SIIFVDEIDSLCGQRGEGNESEASRRIKTELLVQMQGVGNNDQK 876
Query: 313 VMVF-TMNSKENVDPNLLRPGRVDVHIHFPLCDFSSFKTLASNYLGVKDHKL 363
V+V N+ +D + R R D I+ PL D + + + +LG H L
Sbjct: 877 VLVLAATNTPYALDQAIRR--RFDKRIYIPLPDVKARQHMFKVHLGDTPHNL 1026
>TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {Arabidopsis
thaliana;}, partial (87%)
Length = 1385
Score = 48.1 bits (113), Expect = 3e-06
Identities = 44/175 (25%), Positives = 75/175 (42%), Gaps = 18/175 (10%)
Frame = +3
Query: 185 STFETITMETDLKNRVKSDLESFLKGKQYYHRLGRLWKRSFLLYGSSGTGKSSFIAAMAN 244
ST++ I +K +E +K + + LG + LLYG GTGK+ A+A+
Sbjct: 318 STYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 497
Query: 245 FLSYDVYYIDLSRISTD---------SDLKSILLQTAPKSIIVVEDLDRYLTEK------ 289
+ S + +L + + AP SII ++++D + +
Sbjct: 498 HTDCTFIRVSGSELVQKYIGEGSRMVRELFVMAREHAP-SIIFMDEIDSIGSARMESGSG 674
Query: 290 ---SSTTVTSSGILNFMDGIWSGEERVMVFTMNSKENVDPNLLRPGRVDVHIHFP 341
S T +LN +DG + + ++ N + +D LLRPGR+D I FP
Sbjct: 675 XGDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDQALLRPGRIDRKIEFP 839
>TC11760 homologue to UP|PRS4_ORYSA (P46466) 26S protease regulatory subunit
4 homolog (TAT-binding protein homolog 2), partial (27%)
Length = 555
Score = 37.7 bits (86), Expect = 0.004
Identities = 19/39 (48%), Positives = 28/39 (71%)
Frame = +3
Query: 309 GEERVMVFTMNSKENVDPNLLRPGRVDVHIHFPLCDFSS 347
G+ +V++ T +S E++DP LLRPGR+D I FPL D +
Sbjct: 6 GDVKVILAT-HSIESLDPALLRPGRIDRKIEFPLPDIKT 119
>TC9051 UP|PRS7_ARATH (Q9SSB5) 26S protease regulatory subunit 7 (26S
proteasome subunit 7) (26S proteasome AAA-ATPase subunit
RPT1a) (Regulatory particle triple-A ATPase subunit 1a),
partial (46%)
Length = 867
Score = 35.8 bits (81), Expect = 0.015
Identities = 18/49 (36%), Positives = 27/49 (54%)
Frame = +2
Query: 299 ILNFMDGIWSGEERVMVFTMNSKENVDPNLLRPGRVDVHIHFPLCDFSS 347
I+N +DG + ++ N + +DP LLRPGR+D + F L D S
Sbjct: 194 IVNQLDGFDARGNIKVLMATNRPDTLDPALLRPGRLDRKVEFGLPDLES 340
>TC14829 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulatory subunit
6B homolog, partial (28%)
Length = 662
Score = 35.0 bits (79), Expect = 0.026
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +2
Query: 314 MVFTMNSKENVDPNLLRPGRVDVHIHFPLCD 344
++ N + +DP LLRPGR+D I FPL D
Sbjct: 20 VIMATNRADTLDPALLRPGRLDRKIEFPLPD 112
>AV422700
Length = 472
Score = 33.9 bits (76), Expect = 0.058
Identities = 14/27 (51%), Positives = 18/27 (65%)
Frame = +2
Query: 216 RLGRLWKRSFLLYGSSGTGKSSFIAAM 242
+LG W R LLYG GTGK+S + A+
Sbjct: 290 KLGLKWPRGLLLYGPPGTGKTSLVRAV 370
>TC12379 homologue to UP|O04327 (O04327) Cell division protein FtsH isolog,
partial (15%)
Length = 505
Score = 33.9 bits (76), Expect = 0.058
Identities = 25/95 (26%), Positives = 42/95 (43%), Gaps = 11/95 (11%)
Frame = +1
Query: 258 ISTDSDLKSILLQTAPK---SIIVVEDLDRYLTEKS--------STTVTSSGILNFMDGI 306
+S L L Q A + S++ +++LD E+ T + +L +DG
Sbjct: 37 LSLSLSLSRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDATLNQLLVCLDGF 216
Query: 307 WSGEERVMVFTMNSKENVDPNLLRPGRVDVHIHFP 341
E + + + N + +DP L+RPGR D I P
Sbjct: 217 EGRGEVITIASTNRPDILDPALVRPGRFDRKIFIP 321
>TC13255 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (41%)
Length = 532
Score = 32.7 bits (73), Expect = 0.13
Identities = 40/147 (27%), Positives = 66/147 (44%), Gaps = 16/147 (10%)
Frame = +1
Query: 212 QYYHRLGRLWKRSFLLYGSSGTGKSSFIAAMANFLSYDVYYIDLSRIST----DSD-LKS 266
+Y+ L WK LL+G GTGK+ A+A + I S + + DS+ L
Sbjct: 7 KYFTGLLSPWK-GILLFGPPGTGKTMLAKAVATECKTTFFNISASSVVSKWRGDSEKLVK 183
Query: 267 ILLQTA---PKSIIVVEDLDRYL-------TEKSSTTVTSSGILNFMDGIWSGEERVMVF 316
+L Q A S I ++++D + +E ++ + +L MDG+ +E V V
Sbjct: 184 VLFQLARHHAPSTIFLDEIDAIISQRGEARSEHEASRRLKTELLIQMDGLTRTDELVFVL 363
Query: 317 -TMNSKENVDPNLLRPGRVDVHIHFPL 342
N +D +LR R++ I PL
Sbjct: 364 AATNLPWELDAAMLR--RLEKRILVPL 438
>AW719510
Length = 358
Score = 32.7 bits (73), Expect = 0.13
Identities = 17/39 (43%), Positives = 24/39 (60%)
Frame = -2
Query: 309 GEERVMVFTMNSKENVDPNLLRPGRVDVHIHFPLCDFSS 347
G +V++ T N + +DP LLRPGR+D + F L D S
Sbjct: 354 GNIKVLMAT-NRPDTLDPALLRPGRLDRKVEFGLPDLES 241
>TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J8.110 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(29%)
Length = 771
Score = 32.3 bits (72), Expect = 0.17
Identities = 29/125 (23%), Positives = 58/125 (46%), Gaps = 17/125 (13%)
Frame = +3
Query: 223 RSFLLYGSSGTGKSSFIAAMANFLSYDVYYIDLSRISTD---------SDLKSILLQTAP 273
R LL+G GTGK+ A+AN + +S I++ L S+ + AP
Sbjct: 306 RGILLFGPPGTGKTMLAXAIANEAGASFINVSMSTITSKWFGEDEKNVRALFSLAAKVAP 485
Query: 274 KSIIVVEDLDRYLTEKS------STTVTSSGILNFMDGIWS--GEERVMVFTMNSKENVD 325
+II V+++D L +++ + + + DG+ + GE+ +++ N ++D
Sbjct: 486 -TIIFVDEVDSMLGQRTRVGEHEAMRKIKNEFMTHWDGLLTAPGEQILVLAATNRPFDLD 662
Query: 326 PNLLR 330
++R
Sbjct: 663 EAIIR 677
>AV421390
Length = 409
Score = 31.6 bits (70), Expect = 0.29
Identities = 20/74 (27%), Positives = 35/74 (47%)
Frame = +2
Query: 179 VPFTHPSTFETITMETDLKNRVKSDLESFLKGKQYYHRLGRLWKRSFLLYGSSGTGKSSF 238
+P + TF+ + D K ++ +E +L+ + RLG + LL G+ GTGK+
Sbjct: 44 MPEKNVKTFKDVKGCDDAKQELEEVVE-YLRNPAKFTRLGGKLPKGILLTGAPGTGKTLL 220
Query: 239 IAAMANFLSYDVYY 252
A+A +Y
Sbjct: 221 AKAIAGEAGVPFFY 262
>TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulatory subunit
6B homolog, partial (65%)
Length = 901
Score = 31.6 bits (70), Expect = 0.29
Identities = 17/48 (35%), Positives = 25/48 (51%)
Frame = +3
Query: 197 KNRVKSDLESFLKGKQYYHRLGRLWKRSFLLYGSSGTGKSSFIAAMAN 244
K ++ +E L + Y ++G R LLYG GTGK+ A+AN
Sbjct: 525 KQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKAVAN 668
>TC11791 UP|PRSA_LYCES (P54776) 26S protease regulatory subunit 6A homolog
(TAT-binding protein homolog 1) (TBP-1)
(Mg(2+)-dependent ATPase 1) (LEMA-1), partial (29%)
Length = 574
Score = 29.6 bits (65), Expect = 1.1
Identities = 13/36 (36%), Positives = 23/36 (63%), Gaps = 1/36 (2%)
Frame = +2
Query: 307 WSGEERVMVFTMNSKENV-DPNLLRPGRVDVHIHFP 341
+S ++R+ V ++ ++ DP L+R GR+D I FP
Sbjct: 5 FSSDDRIKVIAATNRADILDPALMRSGRLDRKIEFP 112
>TC15037 similar to UP|O24457 (O24457) Pyruvate dehydrogenase E1 alpha
subunit (At1g01090/T25K16_8) , partial (20%)
Length = 499
Score = 29.3 bits (64), Expect = 1.4
Identities = 13/43 (30%), Positives = 29/43 (67%)
Frame = +2
Query: 11 LKIAILLFSIYAIHMIFETGLIHESTKLWRIIEDWFHVYQVFH 53
L+ + + +++A+H+++ETGL+ L+R +E WF V +++
Sbjct: 293 LQFCMEVLNLHAVHLVYETGLV-----LFR-LEIWFLVSYIYY 403
>AV420640
Length = 300
Score = 28.5 bits (62), Expect = 2.4
Identities = 14/48 (29%), Positives = 21/48 (43%)
Frame = -1
Query: 156 IEKQGNRDLRFYMNASDFGPWRFVPFTHPSTFETITMETDLKNRVKSD 203
+EK GN D+R NA PF H T+ + L N +++
Sbjct: 168 VEKDGNSDMRCVFNAISTATITIHPFCHEIICATVRVAVILNNLARAE 25
>CN825587
Length = 663
Score = 28.1 bits (61), Expect = 3.2
Identities = 18/42 (42%), Positives = 25/42 (58%), Gaps = 1/42 (2%)
Frame = +2
Query: 295 TSSGILNFMDGIWSGEERVMVFTMNSKENV-DPNLLRPGRVD 335
T + +L MDG +SG V+V ++ +V D LLRPGR D
Sbjct: 104 TINQLLTEMDG-FSGNSGVIVLAATNRPDVLDSALLRPGRFD 226
>TC14911 homologue to UP|Q93X55 (Q93X55) Peroxin 6 (Fragment), partial (32%)
Length = 1418
Score = 28.1 bits (61), Expect = 3.2
Identities = 16/54 (29%), Positives = 31/54 (56%), Gaps = 1/54 (1%)
Frame = +3
Query: 297 SGILNFMDGIW-SGEERVMVFTMNSKENVDPNLLRPGRVDVHIHFPLCDFSSFK 349
S +L +DG+ S ++ ++ N + +DP LLRPGR D ++ + +S++
Sbjct: 600 SQMLAEIDGLSDSSQDLFIIGASNRPDLIDPALLRPGRFDKLLYVGVNSDASYR 761
>CN825067
Length = 687
Score = 28.1 bits (61), Expect = 3.2
Identities = 28/130 (21%), Positives = 58/130 (44%), Gaps = 10/130 (7%)
Frame = +1
Query: 223 RSFLLYGSSGTGKSSFIAAMANFLSYDVYYI---DLSRISTDSDLK-SILLQTAPKS--- 275
R+ L YG GTGK+ +A D + D++ + + + K L + KS
Sbjct: 193 RNMLFYGPPGTGKTMAARELARKSGLDYALMTGGDVAPLGSQAVTKIHELFDWSKKSKRG 372
Query: 276 -IIVVEDLDRYLTEKSSTTVTSS--GILNFMDGIWSGEERVMVFTMNSKENVDPNLLRPG 332
++ +++ D +L E++ T ++ + LN + + + +V + + D +
Sbjct: 373 LLLFIDEADAFLCERNKTYMSEAQRSALNALLFRTGDQSKDIVLALATNRPGDLDSAVAD 552
Query: 333 RVDVHIHFPL 342
R+D + FPL
Sbjct: 553 RIDEVLEFPL 582
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.138 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,843,215
Number of Sequences: 28460
Number of extensions: 104187
Number of successful extensions: 633
Number of sequences better than 10.0: 55
Number of HSP's better than 10.0 without gapping: 629
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 629
length of query: 468
length of database: 4,897,600
effective HSP length: 94
effective length of query: 374
effective length of database: 2,222,360
effective search space: 831162640
effective search space used: 831162640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC144389.8