BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144345.6 - phase: 0
(360 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14335 similar to UP|MEP1_SOYBN (P29136) Metalloendoproteinase ... 127 2e-30
TC8957 weakly similar to UP|Q9ZUJ5 (Q9ZUJ5) T2K10.2 protein, par... 67 4e-12
TC16638 weakly similar to UP|MEP1_SOYBN (P29136) Metalloendoprot... 59 1e-09
BP078780 48 2e-06
TC11606 weakly similar to UP|Q9LW29 (Q9LW29) Transport inhibitor... 30 0.48
BP080135 30 0.82
TC7989 similar to UP|Q43854 (Q43854) Peroxidase precursor , par... 29 1.4
TC14223 similar to UP|Q8GZV0 (Q8GZV0) Obtusifoliol-14-demethylas... 28 1.8
TC11210 similar to GB|AAP47478.1|32331901|AY164904 RTNLB7 {Oryza... 28 3.1
BP067048 27 6.9
TC13660 similar to UP|Q9SU83 (Q9SU83) Nucleotide pyrophosphatase... 26 9.1
CB828118 26 9.1
>TC14335 similar to UP|MEP1_SOYBN (P29136) Metalloendoproteinase 1 precursor
(SMEP1) , partial (9%)
Length = 1574
Score = 127 bits (320), Expect = 2e-30
Identities = 110/369 (29%), Positives = 169/369 (44%), Gaps = 22/369 (5%)
Frame = +3
Query: 10 FLFLLIIVNTTLSSSFPTLSQFAKPLEYIKKTTEAADYIWDKKIKKLVEDLKDKNLKNIK 69
FLFLL+ SSF + +I E+ K+I ++ + K +K
Sbjct: 60 FLFLLV-------SSFFLFVDVSVSAPFINLAPESGLLTRGKQILSIIGRIPSKYTSTVK 218
Query: 70 PSLPSQLPKY----KGVDQIKQYLSDFGYLEQSGPFNNTLDQETVL-----ALKTYQRYF 120
P K KG++ IK+YL GY++ N+ D + A+ YQ+ F
Sbjct: 219 VIPPQNTIKVGDSVKGLENIKKYLDALGYIKDLVLALNSNDTDKFTEDLKPAIMEYQKNF 398
Query: 121 N------IQQDTLSEILQHIALPRCGVPDRILKYNLTNDISFPKGNQWFPKGTKNLTYGF 174
N I Q+ + ++Q PRCGVPD + N + P W+ K L+Y F
Sbjct: 399 NLPVTGVIDQNLYNTLIQ----PRCGVPD----FTTLNVGTTPAFKPWWKSEKKELSYAF 554
Query: 175 DPRNKIPLDMTNVFRTALTQWSNTTRVLNFTETKSYDDANIKIGFYNITDDDGINDVAVG 234
P+N + D+ VFR A +WSN T LNFTET ++D ++IKI F G V
Sbjct: 555 HPQNNVSDDIRTVFREAFDRWSNETS-LNFTETATFDASDIKIAFVKWDGKGG--RVGAA 725
Query: 235 FTFIVLDSTNVKSGFITLDATKYWALPTEHRGFDLETAAMHQIGHLLGLEHSSDNKSIMY 294
+T + ++ +G + LD + + L MH+IGHLLGL HS + +SIMY
Sbjct: 726 YT-----NYSMHAGGMFLDIDEQYVLLN---------VVMHEIGHLLGLGHSYEKESIMY 863
Query: 295 PTILPSHQKNVQITDSDNLAIQKLY-------SSSTKANANSDDSSGCFKLFGSSSSLLI 347
P ++ ++ + TD D IQK+ +S++ + +D SSG G S
Sbjct: 864 PIVM---REKIDFTDDDRKRIQKVTGNSNNGGTSASSSGTTTDSSSG----GGHDSVWQR 1022
Query: 348 SLSIVFAFV 356
+L + FAF+
Sbjct: 1023NLILGFAFM 1049
>TC8957 weakly similar to UP|Q9ZUJ5 (Q9ZUJ5) T2K10.2 protein, partial (20%)
Length = 724
Score = 67.4 bits (163), Expect = 4e-12
Identities = 48/154 (31%), Positives = 67/154 (43%), Gaps = 26/154 (16%)
Frame = +1
Query: 79 YKGVDQIKQYLSDFGYLEQSGP--FNNTLDQETVLALKTYQRYFN--IQQDTLSEILQHI 134
Y G+ +K Y FGY+ + P F++ D A+KTYQ+ FN I D + L+ I
Sbjct: 265 YDGLSNLKSYFKRFGYIPHAPPSNFSDDFDDALEAAIKTYQKNFNLNITGDLDDKTLRQI 444
Query: 135 ALPRCGVPDRI----------------------LKYNLTNDISFPKGNQWFPKGTKNLTY 172
LPRCGV D I L ++ + + G P G + LTY
Sbjct: 445 MLPRCGVADIINGTTTMNAAGNQDETASHGDSNLHFHTVSHYALFPGQPRGPAGKQELTY 624
Query: 173 GFDPRNKIPLDMTNVFRTALTQWSNTTRVLNFTE 206
F P N + + +VF A +WS T L F E
Sbjct: 625 AFYPGNSLSDAVKSVFTGAFARWSEVT-TLTFRE 723
>TC16638 weakly similar to UP|MEP1_SOYBN (P29136) Metalloendoproteinase 1
precursor (SMEP1) , partial (29%)
Length = 621
Score = 59.3 bits (142), Expect = 1e-09
Identities = 35/89 (39%), Positives = 50/89 (55%), Gaps = 6/89 (6%)
Frame = +2
Query: 247 SGFITLDATKYWALPTE------HRGFDLETAAMHQIGHLLGLEHSSDNKSIMYPTILPS 300
+G LDA + W + + DLE+ A+H+IGHLLGL HSS ++IM+PTI S
Sbjct: 23 NGRFHLDAAEDWVVSGDVSKSALATAVDLESVAVHEIGHLLGLGHSSVEEAIMFPTI-SS 199
Query: 301 HQKNVQITDSDNLAIQKLYSSSTKANANS 329
K V + + D IQ LY ++ N +S
Sbjct: 200 RTKKVVLAEDDIKGIQYLYGTNPSFNGSS 286
>BP078780
Length = 516
Score = 48.1 bits (113), Expect = 2e-06
Identities = 51/173 (29%), Positives = 84/173 (48%), Gaps = 14/173 (8%)
Frame = +3
Query: 2 MKLYQFEFFLFLLII----VNTTLSSSF---PTLSQFAKPL-EYIKKTTEA--ADYIWDK 51
M++ FLFLLI+ V+ ++S SF P L +K + +Y+K+ + +W+K
Sbjct: 30 MRISSHTSFLFLLILSFLLVDVSVSISFAEAPGLLTKSKQIPQYLKQVIASK*TSNVWNK 209
Query: 52 KIKKLVEDLKDKNLKNIKPSLPSQLPKYKGVDQIKQYLSDFGYLEQS--GPFNNTLDQET 109
+K L K + + +I G+ Q++ YL FGYL + F N +
Sbjct: 210 -LKSLFNGPKAR-IGDI----------VDGLSQVQDYLDLFGYLNSTLHSNFTNEYTENA 353
Query: 110 VLALKTYQRYFNIQ-QDTLSEILQHI-ALPRCGVPDRILKYNLTNDISFPKGN 160
A++ +Q+ +N+Q L L H+ + PRCGVPD I N T ++ K N
Sbjct: 354 KSAIEEFQKNYNLQVTGQLDNNLYHVLSQPRCGVPDII---NGTTTMNSRKAN 503
>TC11606 weakly similar to UP|Q9LW29 (Q9LW29) Transport inhibitor
response-like protein, partial (18%)
Length = 892
Score = 30.4 bits (67), Expect = 0.48
Identities = 27/132 (20%), Positives = 53/132 (39%), Gaps = 14/132 (10%)
Frame = -2
Query: 236 TFIVLDSTNVKSGFITLDATKY----------WALPTEHRGFDLETAAMHQIGHLLGLEH 285
T I+L + G+++ + T L +E RGF + + G+L
Sbjct: 861 TVILLSLNSSSKGYLSANTTSQERISAPQFAGTKLKSEKRGFPF-SVTLRMFGNLRVKIS 685
Query: 286 SSDNKSIMYPTILPSHQK----NVQITDSDNLAIQKLYSSSTKANANSDDSSGCFKLFGS 341
T+L H ++Q ++++ +++ T++N +S FK+FGS
Sbjct: 684 GETE*QFPMNTLLLDHLSALYHSLQTSETELRSLRDFTMPRTRSNTSSGKGEAWFKMFGS 505
Query: 342 SSSLLISLSIVF 353
S + L +F
Sbjct: 504 GDSFFLILHSMF 469
>BP080135
Length = 519
Score = 29.6 bits (65), Expect = 0.82
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +3
Query: 15 IIVNTTLSSSFPTLSQFAKPLEYIKKTTEAADYIWDKKIKK 55
++V + SS F TLS++A+P ++A WD++IK+
Sbjct: 327 LLVGSCGSSIFATLSKWARPSALFPCRSQAIPAHWDERIKR 449
>TC7989 similar to UP|Q43854 (Q43854) Peroxidase precursor , partial (85%)
Length = 1352
Score = 28.9 bits (63), Expect = 1.4
Identities = 22/76 (28%), Positives = 35/76 (45%), Gaps = 8/76 (10%)
Frame = +3
Query: 278 GHLLGLEHSSDNKSIMYPTILP----SHQKNVQIT----DSDNLAIQKLYSSSTKANANS 329
GH +G+ H S +YP+ P + +KN+++T ++DN + L S +T N
Sbjct: 642 GHTIGISHCSSFTDRLYPSKDPVMDQTFEKNLKLTCPASNTDNTTVLDLRSPNTFDNKYY 821
Query: 330 DDSSGCFKLFGSSSSL 345
D LF S L
Sbjct: 822 VDLMNRQGLFFSDQDL 869
>TC14223 similar to UP|Q8GZV0 (Q8GZV0) Obtusifoliol-14-demethylase, complete
Length = 1917
Score = 28.5 bits (62), Expect = 1.8
Identities = 17/59 (28%), Positives = 29/59 (48%), Gaps = 5/59 (8%)
Frame = +3
Query: 283 LEHSSDNK----SIMYPTI-LPSHQKNVQITDSDNLAIQKLYSSSTKANANSDDSSGCF 336
L H DN S+++P + +P+H++ Q + +K+ S AN + DD CF
Sbjct: 699 LFHDLDNGMLPISVLFPYLPIPAHKRRDQARKKLSEIFEKIIVSRKSANKSEDDMLQCF 875
>TC11210 similar to GB|AAP47478.1|32331901|AY164904 RTNLB7 {Oryza sativa
(japonica cultivar-group);} , partial (7%)
Length = 602
Score = 27.7 bits (60), Expect = 3.1
Identities = 14/26 (53%), Positives = 17/26 (64%)
Frame = -3
Query: 9 FFLFLLIIVNTTLSSSFPTLSQFAKP 34
F LFLL + T SSFP+ SQF+ P
Sbjct: 120 FPLFLLCFSSPTPCSSFPSSSQFSPP 43
>BP067048
Length = 372
Score = 26.6 bits (57), Expect = 6.9
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = +1
Query: 274 MHQIGHLLGLEHSSDNKSIMYPTILPSHQKNVQITDSDNLAIQKLYSSSTKANANSDDSS 333
M ++ + + S +KS MY TI S+Q+N T ++ +S+ N SS
Sbjct: 172 MEELSNAIFSRPLSSHKSNMYDTISKSYQRNTTTTFQQPSSLSSC*RASSXHN*EPSMSS 351
Query: 334 G 334
G
Sbjct: 352 G 354
>TC13660 similar to UP|Q9SU83 (Q9SU83) Nucleotide pyrophosphatase-like
protein , partial (19%)
Length = 425
Score = 26.2 bits (56), Expect = 9.1
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = +2
Query: 10 FLFLLIIVNTTLSSSFPTLSQFAKPL 35
F FL + +++LSSS P LS A+PL
Sbjct: 140 FAFLFLSSSSSLSSSPPLLSTTARPL 217
>CB828118
Length = 535
Score = 26.2 bits (56), Expect = 9.1
Identities = 15/51 (29%), Positives = 25/51 (48%), Gaps = 4/51 (7%)
Frame = -2
Query: 273 AMHQIGHLLGLEHSSDNKSIMYPTILPSHQKNVQIT----DSDNLAIQKLY 319
++H L L H N + PT+ P+HQ + + ++D L I +LY
Sbjct: 438 SLHNSSFLHQLHHQ*HNFLLSRPTLHPNHQHSAKDLIVS*ENDTLPISELY 286
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,703,151
Number of Sequences: 28460
Number of extensions: 72458
Number of successful extensions: 412
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 402
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 406
length of query: 360
length of database: 4,897,600
effective HSP length: 91
effective length of query: 269
effective length of database: 2,307,740
effective search space: 620782060
effective search space used: 620782060
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC144345.6