BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144345.16 + phase: 0 /pseudo/partial
(221 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC13087 similar to UP|Q9FIF8 (Q9FIF8) Serine protease-like prote... 30 0.43
TC13701 similar to UP|Q9SIR4 (Q9SIR4) At2g25290 protein, partial... 29 0.73
TC17670 weakly similar to UP|WRK2_ARATH (Q9FG77) Probable WRKY t... 28 0.96
TC8557 similar to UP|Q9LK52 (Q9LK52) Dbj|BAA90629.1, complete 28 1.6
TC12549 similar to GB|AAO11541.1|27363244|BT002625 At1g79930/F19... 27 2.8
TC17617 weakly similar to UP|Q84SA8 (Q84SA8) Laccase (Fragment),... 27 2.8
TC9369 similar to UP|Q93X72 (Q93X72) Leucine-rich repeat resista... 27 3.6
TC10727 weakly similar to UP|Q15287 (Q15287) RNA-binding protein... 27 3.6
BP038775 27 3.6
BP060334 27 3.6
BP058134 27 3.6
TC8664 weakly similar to UP|Q9FKW9 (Q9FKW9) Alpha-mannosidase, p... 26 4.7
TC10337 similar to GB|AAL06978.1|15810105|AY056090 AT4g37830/T28... 26 4.7
TC14532 similar to UP|Q9FYA8 (Q9FYA8) SC35-like splicing factor ... 26 4.7
TC13537 similar to GB|AAP21222.1|30102608|BT006414 At3g06035 {Ar... 26 4.7
TC19504 similar to UP|Q9SV89 (Q9SV89) Isoleucine-tRNA ligase-lik... 26 6.2
BP058585 25 8.1
BP069981 25 8.1
TC8844 similar to UP|Q9FNI7 (Q9FNI7) Glucosyltransferase-like pr... 25 8.1
BP049869 25 8.1
>TC13087 similar to UP|Q9FIF8 (Q9FIF8) Serine protease-like protein, partial
(31%)
Length = 1036
Score = 29.6 bits (65), Expect = 0.43
Identities = 14/41 (34%), Positives = 25/41 (60%), Gaps = 2/41 (4%)
Frame = +1
Query: 121 RHTHICRILSIRSSHRFIISTLCRRASLK--CPSMRLPNR* 159
+H+H C++L R+ HR S++C+ + ++ P RL R*
Sbjct: 73 KHSHWCKLLRTRTRHRKRCSSICKDSCIQSMLPKPRLRRR* 195
>TC13701 similar to UP|Q9SIR4 (Q9SIR4) At2g25290 protein, partial (16%)
Length = 432
Score = 28.9 bits (63), Expect = 0.73
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +2
Query: 33 RR*RKIMLRG*SSLPRTTFRRWSLW 57
RR*R +MLR L + F+RW LW
Sbjct: 23 RR*RPMMLRSCLKLLQVNFKRWLLW 97
>TC17670 weakly similar to UP|WRK2_ARATH (Q9FG77) Probable WRKY
transcription factor 2 (WRKY DNA-binding protein 2),
partial (3%)
Length = 525
Score = 28.5 bits (62), Expect = 0.96
Identities = 12/31 (38%), Positives = 16/31 (50%)
Frame = +3
Query: 94 KLKWGWCQLEQVNQWLLLLLSMQLKCLRHTH 124
++KWG C + W L L + L CL TH
Sbjct: 201 QMKWGSCCQRENQMWSLCLNVVALTCLVETH 293
>TC8557 similar to UP|Q9LK52 (Q9LK52) Dbj|BAA90629.1, complete
Length = 1064
Score = 27.7 bits (60), Expect = 1.6
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = -1
Query: 173 KLGLLSLRYRCYMLSCFQLYSREGIVQLDRASLHLIRCLQ 212
++ L S RCY ++C++ ++ I+ +D L + + LQ
Sbjct: 980 QVALTSKHNRCYKVACYRYIHQQ*IIHIDTNKLQINKFLQ 861
>TC12549 similar to GB|AAO11541.1|27363244|BT002625 At1g79930/F19K16_11
{Arabidopsis thaliana;}, partial (25%)
Length = 677
Score = 26.9 bits (58), Expect = 2.8
Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 2/42 (4%)
Frame = +2
Query: 90 TRRKKLKWGW--CQLEQVNQWLLLLLSMQLKCLRHTHICRIL 129
+RRKKLK+ QLE+V +W L++L L + C++L
Sbjct: 122 SRRKKLKFQLPKSQLEKVPRWKLMMLLHHLAPMTMMLTCKML 247
>TC17617 weakly similar to UP|Q84SA8 (Q84SA8) Laccase (Fragment), partial
(53%)
Length = 1139
Score = 26.9 bits (58), Expect = 2.8
Identities = 12/36 (33%), Positives = 24/36 (66%), Gaps = 4/36 (11%)
Frame = +3
Query: 108 WLLLLLSMQLKCLRHTHIC----RILSIRSSHRFII 139
WL LL+++ +KCL +H+ I+++ ++HR I+
Sbjct: 48 WLHLLINLVVKCLIISHLVV*SFMIINMHNTHRHIL 155
>TC9369 similar to UP|Q93X72 (Q93X72) Leucine-rich repeat resistance
protein-like protein, partial (70%)
Length = 859
Score = 26.6 bits (57), Expect = 3.6
Identities = 15/29 (51%), Positives = 17/29 (57%)
Frame = -3
Query: 158 R*NNRCRFNNNNKISKLGLLSLRYRCYML 186
R* RF ++ IS L LL LRYRC L
Sbjct: 239 R*RRSGRFASSLGISPLKLLKLRYRCVKL 153
>TC10727 weakly similar to UP|Q15287 (Q15287) RNA-binding protein
(RNA-binding protein S1, serine-rich domain), partial
(15%)
Length = 932
Score = 26.6 bits (57), Expect = 3.6
Identities = 15/37 (40%), Positives = 23/37 (61%), Gaps = 6/37 (16%)
Frame = -1
Query: 93 KKLKWGWCQLEQVNQWLLLLL------SMQLKCLRHT 123
++ KW CQL +++W++LLL S+Q K RHT
Sbjct: 884 EQAKWIHCQL--LHKWIILLLKKKNLISIQHKLTRHT 780
>BP038775
Length = 524
Score = 26.6 bits (57), Expect = 3.6
Identities = 11/27 (40%), Positives = 14/27 (51%)
Frame = -3
Query: 97 WGWCQLEQVNQWLLLLLSMQLKCLRHT 123
W WC + LLL+ + CLRHT
Sbjct: 225 WDWCPGQIYPSLLLLVCVLLSTCLRHT 145
>BP060334
Length = 339
Score = 26.6 bits (57), Expect = 3.6
Identities = 20/78 (25%), Positives = 33/78 (41%), Gaps = 14/78 (17%)
Frame = +2
Query: 121 RHTHICRILSIRSSHRFIISTLCRRASLKCPSMRLPNR*NNRCRFN-------------- 166
+ + I I+ +SS+R ++ + S C S +R* +R F
Sbjct: 89 KRSRISGIVKSKSSNRILVDEDEWKCSYSCESFLNSSR*TSRNHFQHIKSHCL*QWPALP 268
Query: 167 NNNKISKLGLLSLRYRCY 184
NNN I+ L + RY C+
Sbjct: 269 NNNMITLLHTEAWRYMCW 322
>BP058134
Length = 545
Score = 26.6 bits (57), Expect = 3.6
Identities = 12/35 (34%), Positives = 20/35 (56%)
Frame = +2
Query: 109 LLLLLSMQLKCLRHTHICRILSIRSSHRFIISTLC 143
L LLS+ L+ L+ HIC +L+ + ++ LC
Sbjct: 413 LFFLLSIYLELLKMIHICELLTSSK*IKLMMKMLC 517
>TC8664 weakly similar to UP|Q9FKW9 (Q9FKW9) Alpha-mannosidase, partial
(12%)
Length = 586
Score = 26.2 bits (56), Expect = 4.7
Identities = 16/48 (33%), Positives = 25/48 (51%)
Frame = -1
Query: 93 KKLKWGWCQLEQVNQWLLLLLSMQLKCLRHTHICRILSIRSSHRFIIS 140
+K ++ W Q +Q N+W L L L+ L +C + SS F+IS
Sbjct: 418 RKYEFPWDQAQQQNRWFLQLHP*LLEVL---GLCPLQPSMSSSSFLIS 284
>TC10337 similar to GB|AAL06978.1|15810105|AY056090 AT4g37830/T28I19_110
{Arabidopsis thaliana;}, partial (73%)
Length = 617
Score = 26.2 bits (56), Expect = 4.7
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +1
Query: 117 LKCLRHTHICRILSIRSSHRFIISTL 142
++ LRHTHIC I + R+SH ++ L
Sbjct: 262 MRSLRHTHIC-ISATRNSHGVLMGYL 336
>TC14532 similar to UP|Q9FYA8 (Q9FYA8) SC35-like splicing factor SCL30a, 30a
kD, partial (71%)
Length = 1073
Score = 26.2 bits (56), Expect = 4.7
Identities = 8/19 (42%), Positives = 13/19 (68%)
Frame = -1
Query: 81 RRRGMVMDTTRRKKLKWGW 99
RRRG+++ T R + + W W
Sbjct: 572 RRRGIIVTTARARSVAWWW 516
>TC13537 similar to GB|AAP21222.1|30102608|BT006414 At3g06035 {Arabidopsis
thaliana;} , partial (19%)
Length = 765
Score = 26.2 bits (56), Expect = 4.7
Identities = 20/73 (27%), Positives = 30/73 (40%)
Frame = +3
Query: 54 WSLWEPVLRKPSGMELLCLRKLNLLQVRRRGMVMDTTRRKKLKWGWCQLEQVNQWLLLLL 113
W W P+L P+ + L+C+ L++L + V +R GWC L L L
Sbjct: 327 WPNWNPLLCFPTTLILMCM--LSILTIPST-QVQGLAQR---MIGWCLFSLPTHPLELSL 488
Query: 114 SMQLKCLRHTHIC 126
L C+ C
Sbjct: 489 LKHLLCMLGLSFC 527
>TC19504 similar to UP|Q9SV89 (Q9SV89) Isoleucine-tRNA ligase-like protein
, partial (11%)
Length = 491
Score = 25.8 bits (55), Expect = 6.2
Identities = 11/32 (34%), Positives = 17/32 (52%)
Frame = -1
Query: 104 QVNQWLLLLLSMQLKCLRHTHICRILSIRSSH 135
++ +W LL L M+ CLR +L +R H
Sbjct: 140 RIREWCLLALGMRSPCLRKLPPLLMLLVRFQH 45
>BP058585
Length = 395
Score = 25.4 bits (54), Expect = 8.1
Identities = 14/39 (35%), Positives = 22/39 (55%)
Frame = -3
Query: 44 SSLPRTTFRRWSLWEPVLRKPSGMELLCLRKLNLLQVRR 82
S+L + F+ WSL ++ S M +LC+RK+ L R
Sbjct: 306 SALFKKRFKMWSLLWGLILS*SLMIILCMRKIILFMFFR 190
>BP069981
Length = 409
Score = 25.4 bits (54), Expect = 8.1
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1
Query: 119 CLRHTHICRILSIRSSHRFI 138
C HTHI ILS +SSH I
Sbjct: 166 CSYHTHILVILS*KSSHNSI 225
>TC8844 similar to UP|Q9FNI7 (Q9FNI7) Glucosyltransferase-like protein
(AT5g22740/MDJ22_16), partial (55%)
Length = 1300
Score = 25.4 bits (54), Expect = 8.1
Identities = 20/65 (30%), Positives = 30/65 (45%), Gaps = 7/65 (10%)
Frame = -3
Query: 96 KWGWCQ----LEQVNQWLLLLLSMQLKCLRHTHICRILSIRSSHRFIIS---TLCRRASL 148
+WGW + LE +W ++ L +T+ + S +SSH F S RR L
Sbjct: 275 RWGWLKW*NFLEAKKEW*RIVQISVLSQTHYTNTTLLCSSKSSHYFAHSLGYNEHRRMPL 96
Query: 149 KCPSM 153
CPS+
Sbjct: 95 -CPSI 84
>BP049869
Length = 532
Score = 25.4 bits (54), Expect = 8.1
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = -3
Query: 181 YRCYMLSCFQLYSREGIVQLDRAS 204
+RC LSC L++R G RAS
Sbjct: 266 FRCSRLSCRWLFTRSGFCSRKRAS 195
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.343 0.149 0.500
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,886,886
Number of Sequences: 28460
Number of extensions: 83842
Number of successful extensions: 1062
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 1058
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1062
length of query: 221
length of database: 4,897,600
effective HSP length: 87
effective length of query: 134
effective length of database: 2,421,580
effective search space: 324491720
effective search space used: 324491720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144345.16